Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 19th September.html

From 2013.igem.org

Drug Screening

Thursday 19th September

PCR Verification of vectors

Vectors (pET FprA FdxA, pSB1c3 SirA, and pSB1c3 FprA) were used for colony PCR using DreamTaq. Primers used were:

pET FprA FdxA: MCSI F/R, MCSII F/R, FprA F/R, FdxA F/R

pSB1c3 SirA: SB-prep-3P-1/SB-prep-2Ea, SirA F/R

pSB1c3 FprA: SB-prep-3P-1/SB-prep-2Ea, FprA F/R