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Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 3rd October.html
From 2013.igem.org
Target
ASDFThursday 3rd October
Race to Finish stuff
Plated new SirA, FprA, and FdxA transformations on CHL
Picked pSB1C3 CI and grew in LB broth
Z Score of BL21 dCysI pD002 pD003 with Gentamicin as positive hit control
Analytical Digestions of New Vectors (pAD1S, pAD2C, pAD3K, pAD4A)
Digest pSB1C3 CI with EcoRI and SpeI
Digest pSB3K3 with EcoRI and PstI
Digest pSB1A3 E0240 with XbaI and PstI
Ligation of CI promoter with GFP reporter and pSB3K3 backbone
Heat Shock Transformation of pSB3K3 CI GRP into NEBTurbo
PCR verify SirA, FprA, FdxA, and CI biobrick constructs
