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From 2013.igem.org

Labbook |	Protocols |	Materials |

Contents 1 Antarctic Phosphatase 1.1 About 1.2 Working conditions 1.3 Supplier 1.4 References

Antarctic Phosphatase

About

Antarctic phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.^[1]

Working conditions

Buffer: 1X Antarctic Phosphatase buffer

Supplier

• NEB http://www.neb.com/nebecomm/products/productM0289.asp

References

[1] http://www.neb.com/nebecomm/products/productM0289.asp