Team:UT Dallas/week11

From 2013.igem.org

 9/13

<a name="h.xxtlb18w42ua"></a>Digestion

<a name="h.697azfuj3baj"></a>

<a href="#" name="38959a26c88a3357a15438e9d984f3c3778cb42d"></a><a href="#" name="140"></a><tbody></tbody>

<a name="h.xrogj5s7sqsq"></a>

<a name="h.cl73n6ngob0e"></a>i5-74-14

<a name="h.t8ub7ogf4yl6"></a>i5-74-15

<a name="h.jpsryet65y8"></a>i5-74-16

<a name="h.snj90stfx5zl"></a>i5-74-17

<a name="h.jk4kmjicn50p"></a>i5-25-7

<a name="h.2n9t3yx6c06l"></a>i5-25-7

<a name="h.xvbz2zrnjbwk"></a>DNA

<a name="h.c6jmm016bln3"></a>24

<a name="h.16lrc1qzyued"></a>30

<a name="h.2xdu7ifr8mdm"></a>40

<a name="h.r0blg5pgd7ky"></a>30

<a name="h.trix75fp4o82"></a>24

<a name="h.2s9n7nnm0yt"></a>40

<a name="h.3r2krqea5khz"></a>Buffer

<a name="h.jtwf5eeh1ciu"></a>5

<a name="h.1vmglhdmq7rc"></a>5

<a name="h.wbd6qrtihgp"></a>5

<a name="h.4sir9cuwwb6e"></a>5

<a name="h.4lwzl8ht7bun"></a>5

<a name="h.b2dqr94454ql"></a>5

<a name="h.e5uv7g5mheyi"></a>X

<a name="h.527w7eigph07"></a>1

<a name="h.y6xfz0l6mtq4"></a>1

<a name="h.i3n0xtqefjhh"></a>1

<a name="h.60uje82za5p5"></a>1

<a name="h.3ezt36b46s0g"></a>0

<a name="h.fdm26rb6tszc"></a>0

<a name="h.1bvpb1p6u2y8"></a>P

<a name="h.kpvkhfqtoi0q"></a>1

<a name="h.chnjvvinrjhl"></a>1

<a name="h.346x58nouytm"></a>1

<a name="h.ufvpdkym051h"></a>1

<a name="h.1na5ktpht0ix"></a>1

<a name="h.a6aswy5y5au2"></a>1

<a name="h.p4cuhtxjtior"></a>S

<a name="h.7vibx9gbwc4y"></a>0

<a name="h.h15vdfji96y1"></a>0

<a name="h.a8xy4d6v6e51"></a>0

<a name="h.2hnwmdqdgvqr"></a>0

<a name="h.ri2ufj1ox3y6"></a>1

<a name="h.f12x13dliocs"></a>1

<a name="h.gfzt1h8hkln2"></a>H2O

<a name="h.l72ij0ti43py"></a>19

<a name="h.k4eqpl9yrry"></a>13

<a name="h.khp3loo8q6xr"></a>3

<a name="h.nsz853jqnpi8"></a>13

<a name="h.amyap3hgmdit"></a>12

<a name="h.dg23j86vus9z"></a>3

<a name="h.tdlcd3ohy25t"></a>

<a name="h.qy3m67dtohvf"></a>1

<a name="h.pa0gypdvvg6v"></a>7

<a name="h.e3si8vynkt6w"></a>3

<a name="h.25ye3qjumbxi"></a>4

<a name="h.3qyimjpcq2lr"></a>5

<a name="h.pqwrj3u98077"></a>6

<a name="h.3avtjahfrnif"></a>

<a name="h.8jn55sfnpwm7"></a>Gel Extraction

<a name="h.sy87b0b3s1g0"></a>i5-80-1 → 9.8

<a name="h.oyisq1jins43"></a>

<a name="h.aoosmg8j3n23"></a>Picked from Stock

<a name="h.lcc3zcwulso5"></a>i5-25-7 x 2

<a name="h.c551m5gpzr9t"></a>

<a name="h.j7kii2rvfvjw"></a>Picking colonies from transformation listed on page 78

<a name="h.tmo8kgpxjz8w"></a>i5-78-1 → 0 colonies

<a name="h.frmhc7ompuru"></a>i5-78-2 → 10+ colonies

<a name="h.dirf1dabilro"></a>i5-78-3 → 20+ colonies

<a name="h.g0t4mikj7r65"></a>i5-78-4 → 20+ colonies

<a name="h.sunbh95uk317"></a>i5-78-5 → 100+ colonies → picked 2 colonies→ i5-80-4, i5-80-5

<a name="h.c8wwrznn4ic9"></a>i5-78-6 → 100+ colonies → picked 2 colonies → i5-80-6, i5-80-7

<a name="h.3pqwxu52qk6d"></a>

<a name="h.j6fn8wu17xrc"></a>9/14

<a name="h.gumnb8512hhl"></a>

<a href="#" name="45413af74c1bd6a0166152e04ee7d0e461cc17ee"></a><a href="#" name="141"></a><tbody></tbody>

<a name="h.4hck22vikzor"></a>

<a name="h.brpnz1g820oz"></a>i5-25-7

<a name="h.2mljkk72lda1"></a>i5-25-7

<a name="h.ppznxsjejzjw"></a>DNA

<a name="h.yw0wl1yab7h2"></a>40

<a name="h.c0ecg7qfslxy"></a>40

<a name="h.8df0cbljz1e0"></a>Buffer

<a name="h.luzdg2qr41nw"></a>5

<a name="h.3c303h8lninh"></a>5

<a name="h.faedzhp9ygto"></a>S

<a name="h.rqcgrbxvzj8r"></a>1

<a name="h.dzu79pbbt91w"></a>1

<a name="h.6zx51sihu1rm"></a>P

<a name="h.4iqnj5cy4goj"></a>1

<a name="h.3fv0l0w1qsri"></a>1

<a name="h.98wl9v8iur87"></a>H2O

<a name="h.fr6886llcbpr"></a>3

<a name="h.hfbysk98nnen"></a>3

<a name="h.6ior3m5ygdal"></a>Total

<a name="h.r3nfxuv8lny9"></a>50

<a name="h.uqkoh6fn7i41"></a>50

<a name="h.hx6sra3564qi"></a> 

<a name="h.ivdiui3qs3i5"></a>Miniprep and Glycerol Stock

<a name="h.m07yk96dfrp4"></a>i5-80-2 → i5-81-1        [BB-TT-pFru-RBS-GFP]        187.4

<a name="h.bcuqrki15tzr"></a>i5-80-3 → i5-81-2                (insert)                168.3

<a name="h.s7xbl5stmoti"></a>i5-80-4 → i5-81-3        [BB-CPM-RBS-FruR]        239.4

<a name="h.ldf5ka8738nm"></a>i5-80-5 → i5-81-4                (vector)        706.5

<a name="h.spj4ea4b6j7m"></a>i5-80-5 → i5-81-5        [BB-CPM-RBS-SCR]        161.6

<a name="h.69zqx0htzjml"></a>i5-80-7 → i5-81-6                                128.6

<a name="h.uyyjqdkf32qd"></a>i5-79-3 → i5-81-7        [BB-CPM-RBS-FN-TT-pFru-RBS-GFP]        169.7

<a name="h.ou1stpdj8423"></a>i5-79-4 → i5-81-8                                58.2

<a name="h.nahpqapx1l7u"></a>

<a name="h.wv6we9bajt5z"></a>Digestion

<a href="#" name="e5ce5afb962e8fbed8a4a184d265da3681a65302"></a><a href="#" name="142"></a><tbody></tbody>

<a name="h.by0grscfg47j"></a>

<a name="h.7rkbex67c0vm"></a>i5-81-1

<a name="h.ehnxd3z4v3al"></a>i5-81-2

<a name="h.xnr00fgr1jdg"></a>i5-81-3

<a name="h.pdye74dfvjqn"></a>i5-81-4

<a name="h.68w4zv70mk54"></a>DNA

<a name="h.4axkwcfkzh1h"></a>20

<a name="h.pusw0aw5em4c"></a>20

<a name="h.5plrzllwydwd"></a>15

<a name="h.xrtzac76by0j"></a>20

<a name="h.ob4qcfo9khhh"></a>Buffer

<a name="h.xw77sv2f2ix2"></a>5

<a name="h.2tz7f4t7xp43"></a>5

<a name="h.2518ew9f7r64"></a>5

<a name="h.hnwyveg03ka2"></a>5

<a name="h.hb3c90ho31tp"></a>S

<a name="h.jnxjpb26d3a0"></a>0

<a name="h.nh0ge282tphq"></a>0

<a name="h.wrdain8bmsi6"></a>2

<a name="h.az85w3c5hiri"></a>2

<a name="h.2pdtcwyeumdf"></a>P

<a name="h.o9znamwyi2tv"></a>2

<a name="h.6wbt6jvphoch"></a>2

<a name="h.tatemjpounx4"></a>2

<a name="h.wg1p7buyus6i"></a>2

<a name="h.rugjr5natiin"></a>X

<a name="h.5yb7uyd6vje3"></a>2

<a name="h.3ng70dujbppv"></a>2

<a name="h.tihtelotda84"></a>0

<a name="h.nlfglv2jdu9r"></a>0

<a name="h.9j2gcn866bka"></a>H2O

<a name="h.80o19953hdzf"></a>21

<a name="h.62p3lwt7252l"></a>21

<a name="h.k13f4tmwyc4h"></a>26

<a name="h.97j8xjefzo2h"></a>21

<a name="h.6kdfhihsweo1"></a>Total

<a name="h.ilcafciie9dv"></a>50

<a name="h.yiyw7omvecmb"></a>50

<a name="h.odacu13tynww"></a>50

<a name="h.z0zpggi6fpnj"></a>50

<a name="h.f0f5yje81z19"></a>

<a name="h.e8kalyx9q91x"></a>Test Digestion

<a name="h.o7wiy4vh2qoh"></a>

<a href="#" name="b6ff0e0c71a095be4b834042b35f3a90c6e87be3"></a><a href="#" name="143"></a><tbody></tbody>

<a name="h.sqx6hgkab3u4"></a>

<a name="h.218pnixhp0qm"></a>i5-81-7

<a name="h.nwc6qk1pujy0"></a>i5-81-8

<a name="h.gks26tewt75t"></a>DNA

<a name="h.c24vxbwrrvf1"></a>4

<a name="h.f1igfndlzkes"></a>8

<a name="h.fq5ybgqanzzu"></a>Buffer

<a name="h.a89iwc1ogma2"></a>2

<a name="h.3pxax62bijlm"></a>2

<a name="h.sxuicb5f9msf"></a>E

<a name="h.ixecvufmfnt9"></a>1.5

<a name="h.up2n3neki5vt"></a>1.5

<a name="h.c4odvjd7o3ue"></a>P

<a name="h.z1de3jw1d8nb"></a>1.5

<a name="h.3enyilks7mdu"></a>1.5

<a name="h.7f0412agoss3"></a>H2O

<a name="h.w6lqq84f281d"></a>11

<a name="h.mipv5nizzg9h"></a>7

<a name="h.dbpb6mws3wzt"></a>Total

<a name="h.e05ooajt8k9t"></a>20

<a name="h.u1mubpcdotpv"></a>20

<a name="h.93f2a2r5idxm"></a>

<a name="h.8neadol5bjhi"></a>Gel Extraction

<a name="h.s8s43cm55v8l"></a>i5-25-7 → L 7.8

<a name="h.o31h144lwk8r"></a>i5-25-7 → R 4.5        

<a name="h.ijkggq812b50"></a>

<a name="h.ou91ac5y0haa"></a>vector using L, insert: i5-80-1

<a name="h.cwqsgdtof8gi"></a>

<a name="h.6ciqd4tq3729"></a>Ligation

<a name="h.qwkf1085gajb"></a>

<a href="#" name="382430c9467d90f40362293baa3b5d8d76d29f5d"></a><a href="#" name="144"></a><tbody></tbody>

<a name="h.599bpisk6y7b"></a>

<a name="h.dmxqv342xgmk"></a>2:1

<a name="h.4s0lu5huwann"></a>1:1

<a name="h.69nkorokep3w"></a>0:1

<a name="h.tu4oesjuz1u"></a>Insert

<a name="h.siwsdpbt73fg"></a>11

<a name="h.bz6nzhu2m9g1"></a>7

<a name="h.h5nmbt2y6b04"></a>0

<a name="h.q9e4z9vrfgov"></a>Vector

<a name="h.yy138n42zwo9"></a>4

<a name="h.93t40edvzz7a"></a>7

<a name="h.5zwf5vocvr8q"></a>8

<a name="h.gt9v76irko2o"></a>Buffer

<a name="h.b8e47jhje66g"></a>2

<a name="h.3x3ernmuadm"></a>2

<a name="h.fzl0v5dpj05x"></a>2

<a name="h.m5z5krqilsck"></a>Ligase

<a name="h.32dki2312tkn"></a>2

<a name="h.mv2i70ij00ss"></a>2

<a name="h.4gya1ipar9p3"></a>2

<a name="h.hot8kd37bmy0"></a>H2O

<a name="h.3v11rcsj2d2g"></a>1

<a name="h.j6uuc86ijzh9"></a>2

<a name="h.o1gpxofy3ktk"></a>8

<a name="h.js2ecnrf2umy"></a>Total

<a name="h.vopppb9yat2h"></a>20

<a name="h.scta159bhw2w"></a>20

<a name="h.34loaomkp09"></a>20

<a name="h.y3jzbrn5zi4w"></a>

<a name="h.mpbiwsak0whq"></a>

<a name="h.nx4r1fx40gu6"></a>Redo Test Ligation

<a name="h.7z0zmnrnsu6m"></a>

<a href="#" name="2d6b4b98f4199a2d069d0c85aa3f146efab465f1"></a><a href="#" name="145"></a><tbody></tbody>

<a name="h.35b6m834k67g"></a>

<a name="h.9amhhnmihox9"></a>i5-81-7

<a name="h.d6h8007o1fi"></a>i5-81-8

<a name="h.ctw10sdnd5mn"></a>DNA

<a name="h.d32zn03awdxn"></a>4

<a name="h.x3a0s6ggkfrq"></a>8

<a name="h.3o04wh1xcbs3"></a>Buffer

<a name="h.xkzc8w6rgxan"></a>2

<a name="h.j5d0g3lrb6fk"></a>2

<a name="h.49ig798m7ubt"></a>X

<a name="h.odmazetintdd"></a>1.5

<a name="h.5m87n4nefv2u"></a>1.5

<a name="h.gg581ggdutz8"></a>P-Hf

<a name="h.7r7hcmlwyid7"></a>1.5

<a name="h.n1b31gr9iejq"></a>1.5

<a name="h.5hmr0i1eb15m"></a>H2O

<a name="h.c1ch3arjeku"></a>11

<a name="h.3e3yd7cjjgga"></a>7

<a name="h.jfld0te2ivy"></a>Total

<a name="h.ly0da8xrntu9"></a>20

<a name="h.yr3rrjegzawh"></a>20

<a name="h.o8ujjozau571"></a>

<a name="h.500h2xtbkdd3"></a>Digestion

<a name="h.qzxfrtypd3w"></a>

<a href="#" name="03810063b3d1401792b479aa69bb223f9991c0f9"></a><a href="#" name="146"></a><tbody></tbody>

<a name="h.1ke46k1op6u1"></a>

<a name="h.r6x2khqekpnp"></a>i5-72-5

<a name="h.z1n9evs9xvt2"></a>i5-71-16

<a name="h.jq78mxa7gbm6"></a>i5-68-6

<a name="h.qd6u6qd6g05e"></a>DNA

<a name="h.r6l2dswgjaki"></a>25

<a name="h.o413lfsfp5kc"></a>38

<a name="h.wjtjb0q8m5jk"></a>39

<a name="h.g4whjih1nad"></a>Buffer

<a name="h.k0tlgn4d7bm5"></a>5

<a name="h.63o1idtcv5vs"></a>5

<a name="h.xhglou9q7tlx"></a>5

<a name="h.q6n8b7vaviob"></a>E

<a name="h.916h1mq1ow1y"></a>2

<a name="h.twgzqdoze5ss"></a>2

<a name="h.d5v58tj5dl1r"></a>2

<a name="h.b3d81c3rmg3u"></a>X

<a name="h.lgxvv7pbvdsf"></a>2

<a name="h.5ws2wab38ijo"></a>0

<a name="h.2l84z2lc4rph"></a>0

<a name="h.o1czi5bij6cz"></a>S

<a name="h.n52alg14qs1t"></a>0

<a name="h.szg3q9dszkt0"></a>2

<a name="h.7xqqhigzqvxe"></a>2

<a name="h.si9wbtag9up6"></a>H2O

<a name="h.blqkay7gyd9l"></a>16

<a name="h.voi9lwur0k8"></a>3

<a name="h.mofcfbhmkmx3"></a>2

<a name="h.rc26rb85xkos"></a>Total

<a name="h.yougy8hhg854"></a>50

<a name="h.w2w4d3vsgt4x"></a>50

<a name="h.k7ov1wifh2en"></a>50

<a name="h.iuv4n364djwt"></a>

<a name="h.ndusri5vqehq"></a>1. [BBchlora+CPm-RBS-FRuR-TT-pfru-RBS-GFP]

<a name="h.nf10lidmuv2j"></a>2. i5-83-1 [BBchlora-TT]

<a name="h.5rytqt6atgy0"></a>3. i5-83-2 [Cpu-RBS-FRuR]

<a name="h.6xa5bspt0xhq"></a>4. i5-83-3 [pFru-RBS-GFP]

<a name="h.ci45e2a9zq6o"></a>

<a name="h.djzzbamu01f3"></a>Transformation

<a name="h.j6579u821p8y"></a>2→i5-83-4 → 2 colonies

<a name="h.ihjxgd4xk38y"></a>1 → i5-83-5 → 1 colony

<a name="h.6mpz9i11j1s8"></a>0 → i5-83-6 → 0 colonies

<a name="h.y9jhbdfmuhr8"></a>+ → 40 colonies

<a name="h.vzcuy73c2wye"></a>

<a name="h.vw5hti7d5ao4"></a>Gel Purification:

<a name="h.n69wgee6sqxe"></a>i5-83-1 → 12.8 cut with E/X

<a name="h.fqkmsy5iq02l"></a>i5-83-3 → 5.9 cut with E/S

<a name="h.vwa5lezoxrr"></a>

<a name="h.otpfmaz4cn8o"></a>9/15

<a name="h.35g5ogvo03sr"></a>Picked colonies

<a name="h.r5zu0ailagxz"></a>i5-83-4 → Picked 1 → i5-83-7

<a name="h.dkkcwoxqewoj"></a>i5-83-5 → Picked 1 → i5-83-8

<a name="h.g9hu4l5yua0v"></a>

<a name="h.4bb9c3at4ae9"></a>9/16

<a name="h.evgjm9lljowk"></a>Miniprep/ Nanodrop

<a name="h.4tyguv6rs66m"></a>i5-83-8 → 66.8

<a name="h.bvmf2quze6hh"></a>i5-22-5 → 80.9

<a name="h.tth99r8h1uli"></a>comD → 65.9

<a name="h.gj9axiepm6y9"></a>i5-59-2 → 127.5

<a name="h.w98q2nh34iu"></a>i5-83-7 → 50.0

<a name="h.m4w6qcx4qqqy"></a>i5-35-3 → 103.1

<a name="h.belse8j537ho"></a>comA → 36.5

<a name="h.223kd32u5bj5"></a>i5-22-6 → 269.2

<a name="h.8igupyca0uim"></a>comE→ 146.2

<a name="h.2m22bntac35w"></a>

<a name="h.fxm0tkxpx4iu"></a>Test Digestion

<a name="h.fawqia7fz2gr"></a>

<a href="#" name="70e2cf4d1d74e60b9ee923b6f3d375e5deee5ac3"></a><a href="#" name="147"></a><tbody></tbody>

<a name="h.98eyga2fnpfw"></a>

<a name="h.pvn3yz6dsyn"></a>i5-83-7

<a name="h.cq5yc38ol786"></a>i5-83-8

<a name="h.ten4x1xyc76z"></a>DNA

<a name="h.jbqk6lk1hkjp"></a>4

<a name="h.to9n9o890ygo"></a>4

<a name="h.cp2hobovdn4w"></a>Buffer

<a name="h.dxhhi51wqru9"></a>2

<a name="h.wlis8axr3gu0"></a>2

<a name="h.nkmxfonstelu"></a>E

<a name="h.xxyskh2ep99"></a>1

<a name="h.e79ybpmwuyth"></a>1

<a name="h.lbfgodrmkc03"></a>S

<a name="h.umn4qya8cvhs"></a>1

<a name="h.sudow6ghtspl"></a>1

<a name="h.cjgm4776r07f"></a>H2O

<a name="h.t8f66m5ruttf"></a>12

<a name="h.e9z6bv68jtf"></a>12

<a name="h.wjtun28x2bds"></a>

<a name="h.nfhpaykzz2em"></a>9/17

<a name="h.4tv4sra7dk0m"></a>Miniprep

<a name="h.m4ak8gymwutk"></a>i5-30-1 → 144.6

<a name="h.er6m3csn3d0x"></a>i5-30-1 → 126.0

<a name="h.ifjp9kh99xt4"></a>i5-30-1 → 125.4

<a name="h.y3bofu9jfqan"></a>i5-71-16 → 127.2

<a name="h.9ayfduvl9bk"></a>i5-71-16 → 106.4

<a name="h.dnajds4xx0s1"></a>i5-71-16 → 259.4

<a name="h.bkh0ukgsam0h"></a>

<a name="h.fvum6vplxj8l"></a>Test Digestion

<a name="h.vglmp473kq9e"></a>

<a href="#" name="39a4fd8644497adcb57d7d44d413e6a03e28c9ca"></a><a href="#" name="148"></a><tbody></tbody>

<a name="h.hj4cga853w19"></a>

<a name="h.kwk3qkxhueum"></a>i5-81-2

<a name="h.b1jht5xrjrgr"></a>DNA

<a name="h.24w6w7w60n2b"></a>7

<a name="h.jjeptufat8d9"></a>Buffer

<a name="h.sgv0u8eyrtxs"></a>2

<a name="h.2vryue4rkzw8"></a>E

<a name="h.yishk35wdl6o"></a>1

<a name="h.hrrey0lrbfxp"></a>S

<a name="h.sgwkfnpcauz7"></a>1

<a name="h.la5yokz0x3xh"></a>H2O

<a name="h.vd8s20e7gpbo"></a>9

<a name="h.52im7r60sce"></a>Total

<a name="h.81k6r7ga3mlh"></a>20

<a name="h.ix1yk03ta3dj"></a>

<a name="h.fum15aqpbrpm"></a>Digestion

<a name="h.lvogx7qbtl3f"></a>

<a href="#" name="6c17164fee2dc694c053328c18829f01efce130b"></a><a href="#" name="149"></a><tbody></tbody>

<a name="h.ymqmq269760g"></a>

<a name="h.d21dxpj17uuf"></a>i5-71-16

<a name="h.jx5002fsv53x"></a>i5-81-2

<a name="h.fjl599drvdgi"></a>DNA

<a name="h.238g7moqstb0"></a>33

<a name="h.8y0l3ppdi9f7"></a>21

<a name="h.k87os99s9mwg"></a>Buffer

<a name="h.pd37y3idp1d5"></a>5

<a name="h.nstlsno3xsb1"></a>5

<a name="h.7rsliw3kho5x"></a>E

<a name="h.mmcrz28bc9pp"></a>2.5

<a name="h.c9mnudfllm5e"></a>2.5

<a name="h.jxnpb83pwjq0"></a>S

<a name="h.5juhc25a81gy"></a>2.5

<a name="h.ij69z3j9kom8"></a>0

<a name="h.33hf0hn6q5o1"></a>X

<a name="h.3ooxblkvc7ss"></a>0

<a name="h.ddnlnj6r29q2"></a>2.5

<a name="h.vhgiuokf5rsi"></a>H2O

<a name="h.p4dmlv1ykza9"></a>7

<a name="h.4nfulm49mak8"></a>19

<a name="h.318spdwi8zab"></a>Total

<a name="h.y7snmcdjvp1r"></a>50

<a name="h.o0yldz9ezpm2"></a>50

Retrieved from "http://2013.igem.org/Team:UT_Dallas/week11"