Team:XMU Software/html

2013/7/29 09:16:24	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/7/30 09:18:19	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/7/30 09:19:34	2m19 ai M500
2013/7/30 09:21:18	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/7/30 09:21:31	2M19 M500 AI
2013/7/30 09:22:23	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/7/30 09:23:12	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/7/31 09:23:55	Transformation

2013/8/1 09:24:42	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/1 09:25:15	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/1 09:25:39	AI2M19 1 M500 2 2 IS CORRECT

2013/8/5 21:45:25	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/5 21:45:55	PICK TWO STRAIN
2013/8/6 21:46:26	TRANSFORMATION
2013/8/7 21:50:17	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/7 21:50:56	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/7 21:52:12	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/7 21:52:52	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/8 21:54:37	THE BAND IS UGLY!
2013/8/9 22:05:07	1819(23) pbad2m19 1819(34)1 1819(34)2 1819(35)1 1819(35)2 C C C C C
2013/8/9 22:13:49	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/9 22:14:05	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:07

2013/8/7 21:47:35	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/7 21:48:21	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/7 21:48:47	2 M500 3
2013/8/9 22:02:31	1819(23) pbad2m19 1819(34)1 1819(34)2 1819(35)1 1819(35)2 C C C C C
2013/8/9 22:15:05	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/9 22:15:18	Cultivation Plasmid (A ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s Plasmid (B ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s Plasmid (C ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s
2013/8/10 22:16:21	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:45	PICK TWO STRAIN ONCE MORE

2013/8/8 21:57:12	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/8 22:08:20	CORRECT

2013/8/9 22:09:38	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/9 22:10:21	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/9 22:11:10	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/9 22:11:57	1819(23) 1819 (67)(89)(90) W W C C

2013/8/12 22:22:24	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/12 22:22:43	Cultivation Plasmid (A ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s Plasmid (B ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s Plasmid (C ) Strain antibiotics Medium Volume μL mL L Temperature ℃ Speed rpm rad/s
2013/8/13 23:21:13	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/13 23:21:47	pbad2m19(45)(67) ai2m19 18ai C C C W
2013/8/13 23:25:17	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/13 23:36:12	pbad2m19(45)(65) ai2m19 18ai C C C W

2013/8/15 23:41:42	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/15 23:44:36	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:43:57	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/15 23:47:13	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:52:24	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/15 23:54:43	A12M19,18AI
2013/8/15 23:55:52	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/8/15 23:56:29	Ligation Recommend Actual use A (insert) /μL B (vector) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/16 23:59:03	TRANSFORMATION AND DO THE LIGATION AGAIN
2013/8/17 23:59:37	18AI-AI2M19 1 2 3 4 18AI M ZX WWWWC
2013/8/19 00:07:27	18A-AI2M19 M 18A
2013/8/19 00:09:02	Ligation Recommend Actual use A (insert) /μL B (vector) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/20 00:13:32	18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456) W W W WCCCCC WCWWWC

2013/8/20 00:14:48	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/20 00:15:21	choose the photo you want to submit
2013/8/20 00:16:34	choose the photo you want to submit
2013/8/20 00:16:50	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL

2013/8/21 00:17:45	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/21 00:18:04	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good

2013/8/22 00:18:50	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/22 00:19:07	18o ai2m19 18o1 s d 18o2s C
2013/8/22 00:20:05	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/22 00:20:33	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL

2013/8/23 00:21:30	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/23 00:21:48	18o-ai2m19 1 2 3 M 18A 1 2 WCC C W

2013/8/23 00:23:21	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/23 00:24:04	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/23 00:24:54	WRONG AND USE THE PLASMID TO DO THE DIGESTION
2013/8/23 00:25:28	FAILD

2013/8/23 00:27:17	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/23 00:28:07	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/23 00:29:20	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/8/23 00:29:43	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/24 00:33:05	ALL WRONG
2013/8/25 00:30:23	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/25 00:30:39	TRANSFORMATION
2013/8/26 00:33:48	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/26 00:34:19	PCR-Reaction Reaction /μL dNTP ( Mixture（2.5 mM each） ) /μL Template /μL colony plasmid rTaq /μL H2O /μL
2013/8/26 00:31:30	ALL WRONG
2013/8/28 12:02:00	18O-AI2M19 ALL WRONG

2013/8/29 11:29:30	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:30:51	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:32:21	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:34:47	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:41:40	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:42:42	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/29 11:43:26	ptet 4 6 10 14 16 17
2013/8/29 11:44:18	DO THE LIGAION
2013/8/31 11:44:58	4.1 4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3 17.1 17.2 M 17.2 C C C C C C C W C C W W C W W W W W W PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21) C C C
2013/9/2 11:52:55	4.4 10.4 10.5 10.6 14.3 M 14.4 14.5 16.3 17.1 17.2 ALL WRONG

2013/8/3 18:01:51	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/3 18:02:03	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good
2013/8/3 18:03:36	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/3 18:03:49	OTHER SAMPLE M(500) PBAD WRONG AND DO THIS AGAIN TOMORROW

2013/8/3 18:05:49	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/3 18:08:04	Plasmid Extraction       A260/280 ng/μL (A) (B) (C) Evaluate A260/280>1.8 and >200 ng/μL great fine A260/280<1.6 or <80 ng/μL not good

2013/8/3 18:10:41	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/3 18:11:50	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/3 19:45:37	OTHER SAMPLE M(500) PBAD
2013/8/3 18:13:13	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/8/3 18:13:35	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/3 18:14:18	TRANSFORMATION
2013/8/5 11:54:23	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/8 12:43:18	ANALYSE BY PCR 1819(23) pbad2m19 1819(34)1 1819(34)2 1819(35)1 1819(35)2 C W C C C C
2013/8/9 12:49:26	ANALYSE BY PCR PBAD 1 2 3 4 5 6 C C C

2013/8/10 22:17:31	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/8/10 22:17:54	Extract plasmid EX SP (A) (B) (C) Plasmid EcoR I /μL Xba I /μL Spe I /μL Pst I /μL H20 /μL
2013/8/10 22:18:10	PBAD M1000 2M19
2013/8/10 22:18:46	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/8/10 22:19:07	Ligation Recommend Actual use A (insert) /μL B (vector) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/10 22:19:36	Ligation Recommend Actual use A (insert) /μL B (vector) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/8/10 22:20:43	TRANSFORMATION

2013/8/13 23:25:39	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/13 23:28:09	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/13 23:28:51	pbad2m19(45)(65) ai2m19 18ai C C C W

2013/8/18 00:01:15	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/18 00:02:21	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/18 00:02:55	PCR-Reaction Reaction /μL dNTP ( Mixture（2.5 mM each） ) /μL Template /μL colony plasmid rTaq /μL H2O /μL
2013/8/18 00:05:05	line 1 and line2
2013/8/20 00:11:54	PCR-Reaction Reaction /μL dNTP ( Mixture（2.5 mM each） ) /μL Template /μL colony plasmid rTaq /μL H2O /μL
2013/8/20 00:10:09	18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456) W W W WCCCCC WCWWWC
2013/8/31 11:49:18	4.1 4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3 17.1 17.2 M 17.2 C C C C C C C W C C W W C W W W W W W PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21) C C C

2013/8/26 00:35:54	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/26 00:37:04	PCR-Reaction Reaction /μL dNTP ( Mixture（2.5 mM each） ) /μL Template /μL colony plasmid rTaq /μL H2O /μL
2013/8/26 00:40:19	pbad l4.l5
2013/8/27 00:42:13	TRANSFORMATIOIN
2013/8/27 00:42:54	choose the photo you want to submit
2013/8/28 11:26:54	pbad2m19 line4 5 (1-6) WWWWCC CCCCCC
2013/9/16 12:56:58	THE SEQUNCING SHOWS THE GENE KNOCKOUT IS SUCCESSFUL TRANSLATE IT INTO BL21
2013/9/16 12:58:29	PBAD2M19 LINE 4 IN BL21 123456 ALL CORRECT

2013/8/26 00:39:25	Template and Primers choose plasmid primer1 name plasmid name sequence location direction left right type position part-only sequence primer2 name length sequence backbone name direction left right length position
2013/8/26 00:40:04	PCR-Reaction Reaction /μL dNTP ( Mixture（2.5 mM each） ) /μL Template /μL colony plasmid rTaq /μL H2O /μL
2013/8/26 00:40:51	pbad l4.l5
2013/8/27 00:42:25	TRANSFORMATION
2013/8/28 11:27:57	pbad2m19 line4 5 (1-6) WWWWCC CCCCCC

2013/9/6 12:41:24	Start to Ligate Plasmids choose plasmid (A) plasmid name location type part-only sequence length backbone name length choose plasmid (B) plasmid name location type part-only sequence length backbone name length choose plasmid (C) plasmid name location type part-only sequence length backbone name length
2013/9/6 12:42:40	DIGESTE BY 5uL EcoRI AND PstI
2013/9/6 12:43:17	C3 LINE0 LINE1 LINE2 C W C W
2013/9/6 12:44:27	Electrophoretogram and recover Analyse Electrophoretogram A260/280 ng/μL bp (A) (B) (C) Possible reason: 1. The validity or contamination of gel extraction kit and DD water. 2. The validity of enzyme and buffer 3. Incomplete dissolution of agar gel
2013/9/6 12:44:44	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/9/7 12:48:34	0 AND 2
2013/9/7 12:49:01	Ligation Recommend Actual use A (vector) /μL B (insert) /μL ligase /μL Recommend Actual use A /μL B /μL C /μL ligase /μL H2O /μL
2013/9/7 12:49:19	LINE 1 IN C3 OTHER SAMPLE M C C
2013/9/9 12:50:45	ANALYSE LINE 0 1，2 M LINE 2 1，2