"

From 2013.igem.org

1>Bacteria must be kept in the -80’c circumstance dissolved in the glycerol, and the content of the glycerol is roughly 20% to 30%.
　　　Materials: 40% glycerol, bacteria in the fluid culture.

2>Absorb 600ul bacteria solution and 500ul 40% glycerol, and inject them into the 1.5ml EP tube.

3>Use the marker pen to put down the specific type and relevant resistance of the bacteria, then seal the tube and preserve in the -80’c.