| Risk Group 1: Agents are Not associated with disease in healthy adult humans. (Low risk) |
BSL-1
- Work is done on open bench tops and special containment
equipment is not required.
- Standard microbiological practices are observed.
- Biohazard signs should be posted.
|
Escherichia coli; K12 derivatives (DH5a, JH109, pBluescript, psi2) |
| Risk Group 2: Agents are associated with human disease which is rarely serious. There are often preventive or therapeutic interventions available. (Moderate risk) |
BSL-2
- All BSL-1 containment and practices plus the following:
- Laboratory access is restricted when experimental work is in progress
- Personnel have specific training in handling of agents
- Biological safety cabinets (BSC) or other physical containment devices are used for potential aerosol generation procedures
- Biohazard signs must be posted
- Specific PPE (personnel protective equipment) and entrance requirements
|
- Adenovirus all types; human
- All human blood contaminated specimens; HIV/SIV infected animals
- Human cell lines eg. HEK 293, HeLa
- Herpes Simplex Virus
- Rabies Virus
- Pseudorabies Virus
|
| Risk Group 2 or Risk Group agents; Protocol specific (Moderate to High risk) |
BSL-2+ ("Plus")
- All BSL-2 facilities, containment and practices PLUS BSL-3 practices including:
- Restricted Laboratory access
- Personnel have specific training in handling of agents
- All procedures are performed in biological safety cabinets (BSC)
- Biohazard signs must be posted
- Written safety policies provided by the investigator defining laboratory procedures, waste disposal, disinfection and medical surveillance
- Centrifuge safety cups must be used
|
- Lentiviral vectors, such as:
- SIV; proviral DNA
- HIV-1 infectious molecular clones
|
| Risk Group 3: Agents are associated with serious or lethal human disease for which preventive or therapeutic interventions MAY be available. (High risk) |
BSL-3
- NO current facilities exist to accommodate risk group 3 agents at iGEM
|
None; no current usage of this risk group classification at iGEM |
| Risk Group 4:Agents are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are NOT USUALLY available. (Extreme risk) |
BSL-4
- NO current facilities exist to accommodate risk group 4 agents at iGEM
|
None: no current usage of this risk group classification at iGEM |
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