Team:DTU-Denmark/Methods/Autoclaving

From 2013.igem.org

Autoclaving

Autoclaving in the lab of DTU building 208

  • Close the lid
  • Check if it is locked (the orange pointer should be turned vertical)
  • Wait until the condensation goes out before closing the valve
  • Let temperature reach 125 oC and pressure 20 psi
  • Wait for 15 min before switching off the power and wait an hour.