Lab 208

Main purpose

• Replating of TAT3-1a

Who was in the lab

Henrike, Helen, Ariadni, Kristian, Julia

Procedure

TAT3-1a replating

TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin.

Glucose plates (USER reaction)

Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.

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