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From 2013.igem.org

Update of methods

The list of the methods was slightly updated and the clusters were removed, since they turned out to be not very intuitive. In order to find methods, that are actually mentioned in abstracts we went through those of teams who were successful in either the new application track, experimental measurement aproches and other practice associated directions. The updated list is displayed in table 22.1.
After rerunning the postprocessing of our data sets the top 10 methods list actually contained 10 methods of which many were newly added.

Table 22.1: Updated methods list without clusters

Old	New	Removed
Fusion Proteins, Primer Design, preparation of DNA, Restriction Digestion, Insert preparation, cell fractionation, cell counting, PCR, interaction chromatography, purification, Gel extraction, Ligation, Transformation, FRET, DNA extraction, patch clamp, Northern Blot, Southern Blot, Bioinformatics, ELISA, Chromatography, flow cytometry, X-Ray-crystallography, NMR, Electron microscopy, Molecular dynamics, coimmunoprecipitation, Electrophoretic mobility shift assay, size determination, gel electrophoresis, macromolecule blotting and probing, immuno assays, phenotypic analysis, imaging, spectroscopy, spectrometry	traditional cloning, Gibson, bacterial artificial chromosome, protein interaction, FISH, assembly line, Sequencing, Microarray, computer simulation, drug production, encapsulation, genome deletion, mutagenesis, next generation sequencing, image processing, kill switch, Western Blot, Mass spectrometry, southwestern blot, fluorescence microscopy	cloning, DNA sequencing, DNA Microarray, arrays, Western blotting, southwestern blotting