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From 2013.igem.org

{ "date" : "2013-06-12", "author" : "Gabriele", "title" : "Ligation and transformation", "content" : "Ligation of SAMsynthetase and pSB1C3 with and without RFP, so I prepared four samples:
1) pSB1C3 with RFP (Ctrl_1)
2) pSB1C3 with RFP + SAMsynthetase (1:2)
3) pSB1C3 without RFP (Ctrl_2)
4) pSB1C3 without RFP (1:2)
The samples were prepared and ligation was performed following the ligation protocol.

Transformation in NEB10β cells was performed following the transformation protocol.Plates in incubator ON at 37°C static, more than 16 hours.", "tags" : "SAMsynthetase" }