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From 2013.igem.org

{ "date" : "2013-08-06", "author" : "viola", "title" : "3rd ligation", "content" : "today i saw the plates of the cloning that i did yesterday and tha control plate was a mess... there were too many colonies!!i will do some inocula this evening..i try to redo a new ligation adding the SAP after the digestion of EFE in pUC57. Meanwhile i will start a new overnight digestion trying to insert EFE in the PsB1C3 backbone of the part s04617.", "tags" : "s04617-EFE" }