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Template:Kyoto/Notebook/Sep 28
From 2013.igem.org
Contents |
Sep 28
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | Spinach-DT+GAPDH+A1 |
| 3 | Spinach-DT+Spinach+C1 |
| 4 | Spinach-DT+gfp+E1 |
| 5 | Spinach-DT+aptamer+G1 |
| 6 | Pcon-tetR aptamer-DT+GADPH+A3 |
| 7 | Pcon-tetR aptamer-DT+Spinach+C3 |
| 8 | Pcon-tetR aptamer-DT+gfp+E3 |
| 9 | Pcon-tetR aptamer-DT+aptamer+G3 |
| 10 | Pcon-RBS-GFP-DT+GADPH+A5 |
| 11 | Pcon-RBS-GFP-DT+Spinach+C5 |
| 12 | Pcon-RBS-GFP-DT+gfp+E5 |
| 13 | Pcon-RBS-GFP-DT+aptamer+G5 |
| 14 | 100bp ladder |
| Lane | Sample |
|---|---|
| 1 | Pcon-antisense-Spinach-DT+GAPDH+A7 |
| 2 | Pcon-antisense-Spinach-DT+Spinach+C7 |
| 3 | Pcon-antisense-Spinach-DT+gfp+E7 |
| 4 | Pcon-antisense-Spinach-DT+aptamer+G7 |
| 5 | 100bp ladder |
| 6 | Pcon-Spinach-DT+GAPDH+A9 |
| 7 | Pcon-Spinach-DT+Spinach+C9 |
| 8 | Pcon-Spinach-DT+gfp+E9 |
| 9 | Pcon-Spinach-DT+aptamer+G9 |
Colony PCR
| Sample | base pair |
|---|---|
| 9/27 pT181 attenuatorC(C3)1 | 330 |
| 9/27 pT181 attenuatorC(C3)2 | 330 |
| 9/27 Pcon antisense-DT1 | 372 |
| 9/27 Pcon antisense-DT2 | 372 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 24s |
| Sample | base pair |
|---|---|
| 9/27 Pcon-RBS-GFP-DT (4&5) | 1158 |
| 9/27 Pcon-RBS-GFP-DT (4&5) | 1158 |
| 9/27 Pcon-RBS-GFP-DT (4&5) | 1158 |
| 9/27 Pcon-RBS-GFP-DT (4&5) | 1158 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 1min24s |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | Pcon-tetR aptamer-DT+GADPH+A3 |
| 3 | Pcon-antisense-Spinach-DT+GADPH+A7 |
| 4 | Spinach-DT+GADPH+A1 |
| 5 | Pcon-RBS-GFP-DT+GADPH+A5 |
| 6 | Pcon-Spinach-DT+GADPH+A9 |
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | Pcon-tetR aptamer-DT+gfp+E3 |
| 3 | Pcon-antisense-Spinach-DT+gfp+E7 |
| 4 | Spinach-DT+gfp+E1 |
| 5 | Pcon-RBS-GFP-DT+gfp+E5 |
| 6 | Pcon-Spinach-DT+gfp+E9 |
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | Pcon-tetR aptamer-DT+Spinach+C3 |
| 3 | Pcon-antisense-Spinach-DT+gfp+C7 |
| 4 | Spinach-DT+Spinach+C1 |
| 5 | Pcon-RBS-GFP-DT+Spinach+C5 |
| 6 | Pcon-Spinach-DT+Spinach+C9 |
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | Pcon-tetR aptamer-DT+aptamer+G3 |
| 3 | Pcon-antisense-Spinach-DT+aptamer+G7 |
| 4 | Spinach-DT+aptamer+G1 |
| 5 | Pcon-RBS-GFP-DT+aptamer+G5 |
| 6 | Pcon-Spinach-DT+aptamer+G9 |
