03/09/13

From 2013.igem.org

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* The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest.
* The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest.
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== Result of Miniprep==
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Result of Miniprep Nano Drop
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{| border=1
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|2|| 40|| 62.3|| 1.88|| 2.02   
|2|| 40|| 62.3|| 1.88|| 2.02   
|}
|}
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==Double Digest of the pSB1C3 miniprep==
 +
Samples 1 and 2 were transfered to new eppendorf tubes
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*In each tube it was added
 +
**39ul of DNA (miniprep)
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**1ul of XbaI
 +
**2ul of SpeI
 +
**6ul of cut smart buffer
 +
**12ul of sterile water
 +
The samples were incubated at 37 degrees water buffer
 +
After the samples were incubated at 80 degrees to kill the enzymes

Revision as of 14:09, 3 September 2013

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Miniprep of cells

  • The pSB1C3 RFP cells that were incubated in luria broth overnight yesterday, were minipreped using the OMEGA bio-tek protocol, in order to isolate plasmid of interest.
Result of Miniprep Nano Drop
Samples volume (ul) Concentration (ng/ul)260/280260/230
1 40 56.21.89 1.95
2 40 62.3 1.88 2.02

Double Digest of the pSB1C3 miniprep

Samples 1 and 2 were transfered to new eppendorf tubes

  • In each tube it was added
    • 39ul of DNA (miniprep)
    • 1ul of XbaI
    • 2ul of SpeI
    • 6ul of cut smart buffer
    • 12ul of sterile water

The samples were incubated at 37 degrees water buffer After the samples were incubated at 80 degrees to kill the enzymes