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12/08/13
From 2013.igem.org
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(→Running an agarose gel for sonication analysis) |
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==Running an agarose gel for sonication analysis== | ==Running an agarose gel for sonication analysis== | ||
| - | *To tubes | + | *To tubes 1-6, add 14ul of sterile water and 5ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading. |
| - | *Use 5ul of 1kb marker on | + | *Use 5ul of 1kb marker on both ends |
*Add buffer so that wells in the tray and the tray itself are covered | *Add buffer so that wells in the tray and the tray itself are covered | ||
| - | *Run at | + | *Run at 80V for an hour and a half (gel is 1.2%) |
Revision as of 10:48, 13 August 2013
Sonicating herring sperm DNA
- Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
- Vortex each dilution thoroughly and centrifuge briefly.
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Keep tubes on ice
Running an agarose gel for sonication analysis
- To tubes 1-6, add 14ul of sterile water and 5ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
- Use 5ul of 1kb marker on both ends
- Add buffer so that wells in the tray and the tray itself are covered
- Run at 80V for an hour and a half (gel is 1.2%)
