12/09/13

From 2013.igem.org

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(→Gel Purification of the pGEM-T pasmid double digestions ran in the 11/09/13)
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==Gel Purification of the pGEM-T pasmid double digestions ran in the [[11/09/13]]==
==Gel Purification of the pGEM-T pasmid double digestions ran in the [[11/09/13]]==
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The ge was purified by using the The Zymoclean Gel DNA recovery Kit and its protocol was followed.
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'''Nanodrop Results'''
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{|border=1
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|'''Sample'''||'''volume'''||'''Concentration (ng/ul)'''||'''280/260||'''260/230'''
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|-
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|TODX||48.5||7.1||1.83||0.72
==Double Digest==
==Double Digest==
[[File:doubledigest_gelpurification_12092013.jpg]]
[[File:doubledigest_gelpurification_12092013.jpg]]

Revision as of 20:15, 13 September 2013

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Contents

Isolate plasmid from overnight culture

  • Using Omega mini prep kit protocol
  • Samples:
    • TODF 1,2,3,
    • TODX 1, 2, 3
    • TOBG 1, 2, 3,
    • RFP 1, 2, 3
sampleVolume (ul)Concentration (ng/ul)
TODF143120
TODF24398.8
TODX142399.3
TODX244442.2
TODX343417.5
TOBG143262.4
TOBG247319.2
TOBG341338.4
RFP14177.7
RFP24132.7
RFP34259

Glycerol stocks

  • Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
  • Supernatant was removed
  • Pellet resuspended in 375ul of HMFM
  • Samples were then frozen at -80

Gel Purification of the pGEM-T pasmid double digestions ran in the 11/09/13

The ge was purified by using the The Zymoclean Gel DNA recovery Kit and its protocol was followed.

Nanodrop Results

SamplevolumeConcentration (ng/ul)280/260260/230
TODX48.57.11.830.72

Double Digest

Doubledigest gelpurification 12092013.jpg

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