12/09/13

From 2013.igem.org

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*Samples were then frozen at -80  
*Samples were then frozen at -80  
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==Double Digest==
[[File:doubledigest_gelpurification_12092013.jpg]]
[[File:doubledigest_gelpurification_12092013.jpg]]

Revision as of 09:19, 13 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Isolate plasmid from overnight culture

  • Using Omega mini prep kit protocol
  • Samples:
    • TODF 1,2,3,
    • TODX 1, 2, 3
    • TOBG 1, 2, 3,
    • RFP 1, 2, 3


Glycerol stocks

  • Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
  • Supernatant was removed
  • Pellet resuspended in 375ul of HMFM
  • Samples were then frozen at -80


Double Digest

Doubledigest gelpurification 12092013.jpg