12/09/13

From 2013.igem.org

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(Isolate plasmid from overnight culture)
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*Pellet resuspended in 375ul of HMFM
*Pellet resuspended in 375ul of HMFM
*Samples were then frozen at -80  
*Samples were then frozen at -80  
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==Gel Purification of the pGEM-T pasmid double digestions ran in the [[11/09/13]]==

Revision as of 20:09, 13 September 2013

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Contents

Isolate plasmid from overnight culture

  • Using Omega mini prep kit protocol
  • Samples:
    • TODF 1,2,3,
    • TODX 1, 2, 3
    • TOBG 1, 2, 3,
    • RFP 1, 2, 3
sampleVolume (ul)Concentration (ng/ul)
TODF143120
TODF24398.8
TODX142399.3
TODX244442.2
TODX343417.5
TOBG143262.4
TOBG247319.2
TOBG341338.4
RFP14177.7
RFP24132.7
RFP34259

Glycerol stocks

  • Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
  • Supernatant was removed
  • Pellet resuspended in 375ul of HMFM
  • Samples were then frozen at -80

Gel Purification of the pGEM-T pasmid double digestions ran in the 11/09/13

Double Digest

Doubledigest gelpurification 12092013.jpg