17 August 2013

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Today we did ultrapurification of dsDNA from SELEX 11 that was PCR amplified yesterday (see protocols for details). We used 100 ul of DNA in order to get 50 ul of purified DNA. However, when the purified DNA was checked with PAGE, it was found that this sample is too big to enter the gel. Though, when dsDNA was checked after PCR amplification on PAGE clear 80bp bands appeared. Therefore it was decided to use not purified dsDNA for cloning reaction.