17 July 2013

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Today we decided to amplify DNA from SELEX 11 with PCR. In addition, this will be DNAs will contain A-overhangs in order to be suitable for TOPO TA cloning.
    PCR for making A-overhangs (total 50ul):
  • DNA template 10 - 100 ng
  • 10xPCR buffer 5ul
  • 50mM dNTPs 1ul each
  • Water add to final volume of 49ul
  • Taq Polymerase 1ul
Include a 7-30 min extension at 72degC after the last cycle