20/09/13

From 2013.igem.org

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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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==Miniprep from overnight broths==
==Miniprep from overnight broths==
*DNA is isolated using Omega Miniprep kit and its protocol is followed
*DNA is isolated using Omega Miniprep kit and its protocol is followed

Revision as of 10:08, 20 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Contents

Miniprep from overnight broths

  • DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

SampleVolume ulConcentration ng/ul260/280260/230
B5

Double digest of isolated DNA

  • Digesting with EcorI-HF and PstI-HF to see if insert has been incorporated into DNA

Running on a 1.5% gel

  • Gel pre-made on 18/09/13
  • Running at 100V for 1.5 hours