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From 2013.igem.org

Contents 1 Miniprep from overnight broths 2 Nanodropping the isolated DNA 3 Double digest of isolated DNA 4 Running on a 1.5% gel

Miniprep from overnight broths

• DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

Sample	Volume ul	Concentration ng/ul	260/280	260/230
B5

Double digest of isolated DNA

• Digesting with EcorI-HF and PstI-HF to see if insert has been incorporated into DNA

Running on a 1.5% gel

• Gel pre-made on 18/09/13
• Running at 100V for 1.5 hours