20/09/13

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Miniprep from overnight broths

  • DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

SampleVolume ulConcentration ng/ul260/280260/230
B5

Double digest of isolated DNA

  • Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
  • Gel to be run in the 23/09/13