20/09/13

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Miniprep from overnight broths

  • DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

SampleVolume ulConcentration ng/ul260/280260/230
X129013.51.861.38
X34313.62.121.34
X545162.081.64
X64419.12.041.59
B49311.11.731.28
B5879.51.991.34
B94313.12.141.43
B124314.91.991.23
F19217.61.911.15
F89317.61.851.70
F109112.41.991.30
F124118.52.061.71

Double digest of isolated DNA

  • Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
  • Gel to be run in the 23/09/13