9 September 2013

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Today we did colony pcr of streptavidin colony, using vr2 and vf2 primers.
We have run gel for sel 11 and 12, double bands were there so we did gel extraction.
Streptavidin colony was again inoculated to 2 plates with amp concentrations: 50 and 100 ug/ml
We have minipreps of inp, yfp, rbs, and now need to check them.
One of our supevisers Aliya did dot blot and showed that both antiCEA antibody and protein CEA work well.