Exeter/12 August 2013

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Exeter iGEM 2013 · Paint by Coli

Mini Preps of liquid cultures that worked


NanoDrop

Culture NanoDrop concentration (ng/ul) ul to get 250ng
AMP plasmid 60.0 4.17
Yellow (K592010) 49.1 5.09
RBS + cph8 81.2 3.08
Terminators 27.9 8.96
RFP control 51.3 4.87
RBS + yellow (S05058) 56.7 4.41
OmpF 76.7 3.20
Promoter + RBS 62.6 3.99
Magenta 368.1 0.67
Magenta diluted 35.7 7.0


Culture ul to get 250ng 16ul - answer
AMP plasmid 4.17 11.83
Yellow (K592010) 5.09 10.91
B0015 8.96 7.04
RFP 4.87 11.13
RBS + yellow (S05058) 4.41 11.59
Magenta diluted 7.0 9.0


Culture DNA (pigment) + DNA (term) + DNA plasmid How much DNA is in there +T4 Water
Magenta 0.648 + 0.12 + 2.0 2.768 4.268 5.732
Yellow (K592010) 0.664 + 0.12 + 2.0 2.784 4.284 5.716
Yellow (S05058) 0.680 + 0.12 + 2.0 2.80 4.30 5.70
RFP 0.992 + 0.12 + 2.0 3.112 4.612 5.388


Gel of digest

Lane Content
1 1kb Gene Ruler
2 Yellow (S05058)
3 Magenta
4 Yellow (K592010)
5 RFP
6 AMP plasmid
7 B0015
8 1kb Gene Ruler
Image: 500 pixels

Unfortunately lanes 6 and 7 appear to have not worked. This may be due to human error, or a very low concentration of DNA being pipetted into the wells; the amount is insufficient to show a band.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli