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Exeter/18 July 2013
From 2013.igem.org
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(→NanoDrop data from yesterday's gel extraction) |
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==Re-streak plating== | ==Re-streak plating== | ||
| - | We re-streak plated | + | We re-streak plated all of the plates that were more than 10 days old: |
| + | |||
| + | Magenta pigment (K592012) | ||
| + | |||
| + | OmpR promoter | ||
| + | |||
| + | RBS (B0034) PSB1A2 | ||
| + | |||
| + | Terminator (B0015) PSB1L3 | ||
| + | |||
| + | Blue sensor (K592004) PSB1C3 | ||
| + | |||
| + | Promoter + RBS (K608002) PSB1C3 | ||
| + | |||
| + | CcaS (K592001) | ||
| + | |||
| + | RFP control | ||
| + | |||
| + | FixJ intermediate (K592005) | ||
| + | |||
| + | CcaR | ||
| + | |||
| + | GFP | ||
| + | |||
| + | Yellow pigment (K592010) | ||
| + | |||
| + | *NOTE : The GFP and yellow pigment plates got muddled up before they were labelled and had to be streaked the next day (19th July). | ||
| + | |||
| + | *Note : When the RFP control had been incubated over night it had turned a magenta/red colour. | ||
TO BE CONTINUED | TO BE CONTINUED | ||
Revision as of 10:16, 19 July 2013
NanoDrop data from yesterday's gel extraction
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- Yellow pigment (BBa_K592010) - 6.6ng/ul
- Magenta pigment (BBa_K592012) - 4.9ng/ul
- Green light sensor, CcaS (BBa_K592001) - 3.2ng/ul
- RFP control - 4.4ng/ul
- New Part 2, promoter and RBS added to FixJ - 2.1ng/ul
- Blue light sensor, YF1 (BBa_K592004) - 1.4ng/ul
- FixJ protein intermediate, (BBa_K592005) - 10.0ng/ul
- Kanamycin resistant plasmid - 2.0ng/ul
Re-streak plating
We re-streak plated all of the plates that were more than 10 days old:
Magenta pigment (K592012)
OmpR promoter
RBS (B0034) PSB1A2
Terminator (B0015) PSB1L3
Blue sensor (K592004) PSB1C3
Promoter + RBS (K608002) PSB1C3
CcaS (K592001)
RFP control
FixJ intermediate (K592005)
CcaR
GFP
Yellow pigment (K592010)
- NOTE : The GFP and yellow pigment plates got muddled up before they were labelled and had to be streaked the next day (19th July).
- Note : When the RFP control had been incubated over night it had turned a magenta/red colour.
TO BE CONTINUED
