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Exeter/18 July 2013
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Revision as of 08:57, 22 July 2013 by Fentwistle (Talk | contribs)
NanoDrop data from yesterday's gel extraction
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- Yellow pigment (BBa_K592010) - 6.6ng/ul
- Magenta pigment (BBa_K592012) - 4.9ng/ul
- Green light sensor, CcaS (BBa_K592001) - 3.2ng/ul
- RFP control - 4.4ng/ul
- New Part 2, promoter and RBS added to FixJ - 2.1ng/ul
- Blue light sensor, YF1 (BBa_K592004) - 1.4ng/ul
- FixJ protein intermediate, (BBa_K592005) - 10.0ng/ul
- Kanamycin resistant plasmid - 2.0ng/ul
These concentrations are reasonablt low, so the SureClean protocol from 17/7/13 was implemented.
| Part | First concentration (ng/ul) | New concentration (ng/ul) |
|---|---|---|
| Yellow pigment | 6.6 | 168.7 |
| Magenta pigment | 4.9 | 154.6 |
| CcaS (green light sensor) | 3.2 | 81.1 |
| "New Part 2" (promoter, RBS and FixJ) | 2.1 | 207.6 |
| YF1 (blue light sensor) | 1.4 | 172.8 |
| FixJ | 10.0 | 155.8 |
Re-streak plating
We re-streak plated all of the plates that were more than 10 days old, which were:
- Magenta pigment (K592012)
- OmpR promoter
- RBS (B0034) PSB1A2
- Terminator (B0015) PSB1L3
- Blue sensor (K592004) PSB1C3
- Promoter + RBS (K608002) PSB1C3
- CcaS (K592001)
- RFP control
- FixJ intermediate (K592005)
- CcaR
- GFP
- Yellow pigment (K592010)
- NOTE : The GFP and yellow pigment plates got muddled up before they were labelled and had to be streaked the next day (19th July).
- NOTE : When the RFP control had been incubated over night it had turned a magenta/red colour.
TO BE CONTINUED
