http://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&feed=atom&action=historyExeter/3 July 2013 - Revision history2024-03-28T23:02:10ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=251903&oldid=prevFentwistle at 07:02, 30 September 20132013-09-30T07:02:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Made stock solutions of chloramphenicol, ampicillin and kanamycin==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Made stock solutions of chloramphenicol, ampicillin and kanamycin==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].</ins></div></td></tr>
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</table>Fentwistlehttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=58970&oldid=prevFelicityHeath: /* Making stock solutions of chloramphenicol, ampicillin and kanamycin */2013-08-07T14:45:39Z<p><span class="autocomment">Making stock solutions of chloramphenicol, ampicillin and kanamycin</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:45, 7 August 2013</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">Making </del>stock solutions of chloramphenicol, ampicillin and kanamycin==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Made </ins>stock solutions of chloramphenicol, ampicillin and kanamycin==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td></tr>
</table>FelicityHeathhttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=37343&oldid=prevFentwistle at 10:35, 22 July 20132013-07-22T10:35:06Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Making stock solutions of chloramphenicol, ampicillin and kanamycin<del class="diffchange diffchange-inline">'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==</ins>Making stock solutions of chloramphenicol, ampicillin and kanamycin<ins class="diffchange diffchange-inline">==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Weigh each antibiotic into an Eppendorf tube, then transfer into a 10 ml Falcon tube. Add 10 ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Weigh each antibiotic into an Eppendorf tube, then transfer into a 10 ml Falcon tube. Add 10 ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Making antibiotic LB agar plates<del class="diffchange diffchange-inline">'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==</ins>Making antibiotic LB agar plates<ins class="diffchange diffchange-inline">==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will be using the 200 ml Duran bottles of LB agar we made yesterday.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We will be using the 200 ml Duran bottles of LB agar we made yesterday.</div></td></tr>
</table>Fentwistlehttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=35986&oldid=prevAdamThomas at 10:20, 19 July 20132013-07-19T10:20:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Place the bottle in the microwave (with the lid off!) for approximately 5 minutes. Keep an eye on it, they bubble up very easily. Remove from the microwave every so often and swirl to help the agar dissolve.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Place the bottle in the microwave (with the lid off!) for approximately 5 minutes. Keep an eye on it, they bubble up very easily. Remove from the microwave every so often and swirl to help the agar dissolve.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For each 200 ml of LB agar, 200 <del class="diffchange diffchange-inline">ul </del>of antibiotic stock solution needs to be added. This can only be done when the agar has cooled sufficiently, approximately below <del class="diffchange diffchange-inline">50oC</del>. 200ml of agar should make about 8 plates of normal thickness.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For each 200 ml of LB agar, 200 <ins class="diffchange diffchange-inline">&micro;l </ins>of antibiotic stock solution needs to be added. This can only be done when the agar has cooled sufficiently, approximately below <ins class="diffchange diffchange-inline">50&deg;C</ins>. 200ml of agar should make about 8 plates of normal thickness.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td></tr>
</table>AdamThomashttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=32166&oldid=prevJoshMiddleton at 10:41, 16 July 20132013-07-16T10:41:36Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:41, 16 July 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Ampicillin, stock solution is <del class="diffchange diffchange-inline">100mg</del>/ml, so for 10ml we need <del class="diffchange diffchange-inline">1000mg </del>(<del class="diffchange diffchange-inline">1g</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Ampicillin, stock solution is <ins class="diffchange diffchange-inline">100 mg</ins>/ml, so for 10ml we need <ins class="diffchange diffchange-inline">1000 mg </ins>(<ins class="diffchange diffchange-inline">1 g</ins>)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Chloramphenicol, stock solution is <del class="diffchange diffchange-inline">35mg</del>/ml, so we need 350mg</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Chloramphenicol, stock solution is <ins class="diffchange diffchange-inline">35 mg</ins>/ml, so we need 350mg</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Kanamycin, stock solution is <del class="diffchange diffchange-inline">50mg</del>/ml, so we need <del class="diffchange diffchange-inline">500mg</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Kanamycin, stock solution is <ins class="diffchange diffchange-inline">50 mg</ins>/ml, so we need <ins class="diffchange diffchange-inline">500 mg</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Weigh each antibiotic into an Eppendorf tube, then transfer into a <del class="diffchange diffchange-inline">10ml </del>Falcon tube. Add <del class="diffchange diffchange-inline">10ml </del>ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Weigh each antibiotic into an Eppendorf tube, then transfer into a <ins class="diffchange diffchange-inline">10 ml </ins>Falcon tube. Add <ins class="diffchange diffchange-inline">10 ml </ins>ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Making antibiotic LB agar plates'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Making antibiotic LB agar plates'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We will be using the <del class="diffchange diffchange-inline">200ml </del>Duran bottles of LB agar we made yesterday.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We will be using the <ins class="diffchange diffchange-inline">200 ml </ins>Duran bottles of LB agar we made yesterday.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Place the bottle in the microwave (with the lid off!) for approximately 5 minutes. Keep an eye on it, they bubble up very easily. Remove from the microwave every so often and swirl to help the agar dissolve.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Place the bottle in the microwave (with the lid off!) for approximately 5 minutes. Keep an eye on it, they bubble up very easily. Remove from the microwave every so often and swirl to help the agar dissolve.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For each <del class="diffchange diffchange-inline">200ml </del>of LB agar, <del class="diffchange diffchange-inline">200ul </del>of antibiotic stock solution needs to be added. This can only be done when the agar has cooled sufficiently, approximately below 50oC. 200ml of agar should make about 8 plates of normal thickness.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For each <ins class="diffchange diffchange-inline">200 ml </ins>of LB agar, <ins class="diffchange diffchange-inline">200 ul </ins>of antibiotic stock solution needs to be added. This can only be done when the agar has cooled sufficiently, approximately below 50oC. 200ml of agar should make about 8 plates of normal thickness.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</div></td></tr>
</table>JoshMiddletonhttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=30958&oldid=prevFentwistle at 11:26, 15 July 20132013-07-15T11:26:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Making antibiotic LB agar plates'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Making antibiotic LB agar plates'''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We will be using the 200ml Duran bottles of LB agar we made yesterday.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Place the bottle in the microwave (with the lid off!) for approximately 5 minutes. Keep an eye on it, they bubble up very easily. Remove from the microwave every so often and swirl to help the agar dissolve.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For each 200ml of LB agar, 200ul of antibiotic stock solution needs to be added. This can only be done when the agar has cooled sufficiently, approximately below 50oC. 200ml of agar should make about 8 plates of normal thickness.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Add the antibiotic to the agar in a fume hood, swirl gently and pour each plate. The plates need to be left in the fume hood for ~20 minutes to solidify.</ins></div></td></tr>
</table>Fentwistlehttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=30950&oldid=prevFentwistle at 11:19, 15 July 20132013-07-15T11:19:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Kanamycin, stock solution is 50mg/ml, so we need 500mg</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Kanamycin, stock solution is 50mg/ml, so we need 500mg</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water. </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Weigh each antibiotic into an Eppendorf tube, then transfer into a 10ml Falcon tube. Add 10ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Making antibiotic LB agar plates'''</ins></div></td></tr>
</table>Fentwistlehttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=30949&oldid=prevFentwistle at 11:17, 15 July 20132013-07-15T11:17:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">- Ampicillin, stock solution is 100mg/ml, so for 10ml we need 1000mg (1g)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">- Chloramphenicol, stock solution is 35mg/ml, so we need 350mg</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">- Kanamycin, stock solution is 50mg/ml, so we need 500mg</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water.</ins></div></td></tr>
</table>Fentwistlehttp://2013.igem.org/wiki/index.php?title=Exeter/3_July_2013&diff=30942&oldid=prevFentwistle: Created page with "'''Making stock solutions of chloramphenicol, ampicillin and kanamycin''' Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We ..."2013-07-15T11:11:06Z<p>Created page with "'''Making stock solutions of chloramphenicol, ampicillin and kanamycin''' Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We ..."</p>
<p><b>New page</b></p><div>'''Making stock solutions of chloramphenicol, ampicillin and kanamycin'''<br />
<br />
Chloramphenicol and kanamycin are '''TOXIC''' so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).</div>Fentwistle