Exeter/3 September 2013

From 2013.igem.org

(Difference between revisions)
Line 82: Line 82:
! Part !! Concentration (ng/ul)
! Part !! Concentration (ng/ul)
|-
|-
-
| gBlock 1 ||324.6
+
| gBlock 1 || 27.4
|-
|-
-
| gBlock 2 || 264.4
+
| gBlock 2 || 28.6
|-
|-
-
| gBlock 3 || 64.3
+
| gBlock 3 || 101.2
|-
|-
-
| gBlock 4 || 102.7
+
| gBlock 4 || 37.2
|-
|-
-
| gBlock 5 || 48.9
+
| gBlock 5 || 168.6
|-
|-
-
| gBlock 6 || 21.5
+
| gBlock 6 || 21.3
|-
|-
-
| gBlock 7 || 22.6
+
| gBlock 7 || 153.7
|-
|-
-
| gBlock 8 || 22.0
+
| gBlock 8 || 30.8
|-
|-
-
| gBlock 9 || 31.9
+
| gBlock 9 || 247.1
|-
|-
-
| gBlock 10 || 35.4
+
| gBlock 10 || 183.4
|-
|-
-
| gBlock 11 || 27.1
+
| gBlock 11 || 197.4
|-
|-
-
| gBlock 12 || 28.2
+
| gBlock 12 || 105.1
|-
|-
-
| gBlock 13 || 31.9
+
| gBlock 13 || 126.6
|-
|-
-
| gBlock 14 || 35.4
+
| gBlock 14 || 172.5
|-
|-
-
| gBlock 15 || 27.1
+
| gBlock 15 || 87.0
|-
|-
-
| OmpF + RBS A || 35.4
+
| OmpF + RBS A || 41.2
|-
|-
-
| OmpF + RBS B || 27.1
+
| OmpF + RBS B || 57.8
|-
|-
-
| OmpF + RBS C || 28.2
+
| OmpF + RBS C || 74.2
|-
|-
-
| OmpF + RBS D || 31.9
+
| OmpF + RBS D || 46.7
|-
|-
-
| K592011 B|| 35.4
+
| K592011 B || 159.0
|}
|}

Revision as of 14:49, 3 September 2013

Contents

Results of liquid cultures from yesterday

gBlocks

colours of our liquid cultures
gBlock Colour
1 No
2 No
3 Vibrant pink
4 No
5 Vibrant pink
6 No
7 Peach
8 No
9 Pink - not so vibrant
10 Vibrant pink
11 Vibrant pink
12 Pink - not so vibrant
13 Pink - not so vibrant
14 No
15 No


Part Did it work?
OmpF + RBS A ✓
OmpF + RBS B ✓
OmpF + RBS C ✓
OmpF + RBS D ✓
K592022 A ✗
K592022 B ✓
K592011 A ✗
K592011 B ✓
K864404 ✓


Miniprepped

  • OmpF + RBS A
  • OmpF + RBS B
  • OmpF + RBS C
  • OmpF + RBS D
  • K592011 B
  • gblocks 1 to 15

NanoDrop from miniprep

Part Concentration (ng/ul)
gBlock 1 27.4
gBlock 2 28.6
gBlock 3 101.2
gBlock 4 37.2
gBlock 5 168.6
gBlock 6 21.3
gBlock 7 153.7
gBlock 8 30.8
gBlock 9 247.1
gBlock 10 183.4
gBlock 11 197.4
gBlock 12 105.1
gBlock 13 126.6
gBlock 14 172.5
gBlock 15 87.0
OmpF + RBS A 41.2
OmpF + RBS B 57.8
OmpF + RBS C 74.2
OmpF + RBS D 46.7
K592011 B 159.0
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