Exeter/5 August 2013

From 2013.igem.org

(Difference between revisions)
(Digestion)
Line 39: Line 39:
! Part !! DNA (ul) !! dH20 (ul) !! Buffer (ul) !! BSA (ul) !! Enzyme 1 (ul) !! Enzyme 2 (ul) !! Enzyme 3 (ul)
! Part !! DNA (ul) !! dH20 (ul) !! Buffer (ul) !! BSA (ul) !! Enzyme 1 (ul) !! Enzyme 2 (ul) !! Enzyme 3 (ul)
|-
|-
-
| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
+
| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
|-
|-
-
| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
+
| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
|-
|-
-
| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || Xbal 0.5 || Pstl 0.5 || -
+
| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || <i>XbaI</i> 0.5 || <i>PstI</i> 0.5 || -
|-
|-
-
| AMP plasmid || 10 || 6 || 2.5 || 0.5 || EcoR1 0.5 || Pst1 0.5 || Dpnl 0.5
+
| AMP plasmid || 10 || 6 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || Dpnl 0.5
|-
|-
-
| RFP || 2.6 || 13.4 || 2.5 || 0.5 || EcoR1 0.5 || Pstl 0.5 || -  
+
| RFP || 2.6 || 13.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || -  
|}
|}
-
 
=== Ligation ===
=== Ligation ===

Revision as of 20:00, 1 October 2013

Exeter iGEM 2013 · Paint by Coli

Glycerol stocks

We made up 3 replicates of:

K592018

S05058

B0015


Nano Drop

Culture NanoDrop concentration (ng/ul) size
K592018 160.7 2526bp
S05058 118.3 717bp
RFP 96.1 1047bp
B0015 76.3 129bp


Digestion

Part DNA (ul) dH20 (ul) Buffer (ul) BSA (ul) Enzyme 1 (ul) Enzyme 2 (ul) Enzyme 3 (ul)
K592018 1.6 14.4 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
S05058 2.1 13.9 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
B0015 3.3 12.7 2.5 0.5 XbaI 0.5 PstI 0.5 -
AMP plasmid 10 6 2.5 0.5 EcoRI 0.5 PstI 0.5 Dpnl 0.5
RFP 2.6 13.4 2.5 0.5 EcoRI 0.5 PstI 0.5 -

Ligation

Two methods using 'standard' amounts of each digest and using equimolar amounts.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli