Exeter/8 August 2013

From 2013.igem.org

(Difference between revisions)
(Liquid Cultures, Miniprepped)
 
Line 11: Line 11:
* [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
* [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
-
* [http://parts.igem.org/Part:BBa_R0082 R0082] (OmpF promoter) CAM
+
* [http://parts.igem.org/Part:BBa_R0082 R0082] (''ompC'' promoter) CAM
* DH5α 3 (Tom and Adam's control) TET
* DH5α 3 (Tom and Adam's control) TET
Line 63: Line 63:
| RFP || 42.9
| RFP || 42.9
|-
|-
-
| OmpF || 117.3
+
| ''ompC'' || 117.3
|-
|-
| DH5α 3 || 46.3
| DH5α 3 || 46.3

Latest revision as of 20:38, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Liquid Cultures, Miniprepped

  • [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
  • [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
  • [http://parts.igem.org/Part:BBa_R0082 R0082] (ompC promoter) CAM
  • DH5α 3 (Tom and Adam's control) TET
  • DH5α 5 (Tom and Adam's RBS + Cyan) TET
  • DH5α 1 (Tom and Adam's RBS + cph8) TET
  • [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
  • [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
  • [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + cph8) CAM
  • [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (RBS + promoter) CAM
  • [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
  • [http://parts.igem.org/Part:BBa_K322124 K322124] (RBS + cph8) CAM

( 3 replicates, #2, #3 and #4.)

NanoDropping Promoter + RBS Replicates

1 = 29.6ng/ul

1 = 29.2ng/ul

2 = 12.2ng/ul

2 = 12.5ng/ul

3 = 22.8ng/ul

3 = 23.6ng/ul

4 = 17.8ng/ul

4 = 17.5ng/ul


NanoDrop of liquid cultures

Liquid culture NanoDrop result (ng/ul)
Magenta 106.9
RFP 42.9
ompC 117.3
DH5α 3 46.3
S05058 67.1
Yellow 57.1
RBS + cph8 113.7
cph8 #4 49.2 and 48.8
cph8 #2 221.9 and 180.7
cph8 #3 70.8 and 70.8
Promoter and RBS 99.9
B0015 52.1
AMP plasmid 82.5


We sent off cph8 K322124 red light sensor for sequencing.


2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli