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Exeter/9 July 2013
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'''Our requested DNA has arrived from iGEM!''' | '''Our requested DNA has arrived from iGEM!''' | ||
The Parts sent over were... | The Parts sent over were... | ||
| - | - Magenta pigment coding (BBa_K592012, pSB1C3) | + | - Magenta pigment coding ([http://parts.igem.org/Part:BBa_K592012 BBa_K592012], pSB1C3) |
| - | - Green light sensor (BBa_K592001, pSB1C3) | + | - Green light sensor ([http://parts.igem.org/Part:BBa_K592001 BBa_K592001], pSB1C3) |
| - | - Yellow pigment coding (BBa_K592010, pSB1C3) | + | - Yellow pigment coding ([http://parts.igem.org/Part:BBa_K592010 BBa_K592010], pSB1C3) |
| - | - Cyan pigment coding (BBa_K322122, pSB1C3) | + | - Cyan pigment coding ([http://parts.igem.org/Part:BBa_K322122 BBa_K322122], pSB1C3) |
| - | - FixJ intermediate protein coding (BBa_K592005, pSB1C3) | + | - FixJ intermediate protein coding ([http://parts.igem.org/Part:BBa_K592005 BBa_K592005], pSB1C3) |
'''We also requested some extra parts from the Registry''' | '''We also requested some extra parts from the Registry''' | ||
| - | - 2 Genome Integration Kits (BBa_K510000 and BBa_K510012). We know we want to genome integrate some of our Parts at some point... | + | - 2 Genome Integration Kits ([http://parts.igem.org/Part:BBa_K510000 BBa_K510000] and [http://parts.igem.org/Part:BBa_K510012 BBa_K510012]). We know we want to genome integrate some of our Parts at some point... |
| - | - Alternative cyan pigments (BBa_K592011 just codes for the pigment but has no promoter, RBS or terminator. BBa_K864404 codes for the same pigment, but has a promoter and RBS. BBa_K592022 also has the same coding region and RBS, but an alternative promoter) | + | - Alternative cyan pigments ([http://parts.igem.org/Part:BBa_K592011 BBa_K592011] just codes for the pigment but has no promoter, RBS or terminator. [http://parts.igem.org/Part:BBa_K864404 BBa_K864404] codes for the same pigment, but has a promoter and RBS. [http://parts.igem.org/Part:BBa_K592022 BBa_K592022] also has the same coding region and RBS, but an alternative promoter) |
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We are transforming... | We are transforming... | ||
| - | - BBa_K608002, codes for a promoter and RBS | + | - [http://parts.igem.org/Part:BBa_K608002 BBa_K608002], codes for a promoter and RBS |
| - | - BBa_K592004, our blue light sensor | + | - [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], our blue light sensor |
| - | - BBa_B0015, a terminator | + | - [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator |
| - | - BBa_B0034, an RBS | + | - [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 BBa_B0034], an RBS |
| - | - K592006, the promoter that is activated by FixJ | + | - [http://parts.igem.org/Part:BBa_K592006 K592006], the promoter that is activated by FixJ |
We already have the following Parts as LB stab plates from the iGEM registry, so a transformation of these Parts is required: | We already have the following Parts as LB stab plates from the iGEM registry, so a transformation of these Parts is required: | ||
| - | - BBa_K592005, codes for our intermediate protein, FixJ | + | - [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], codes for our intermediate protein, FixJ |
| - | - BBa_K592010, our yellow pigment | + | - [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], our yellow pigment |
The transformation protocol from 4/7/13 was followed, no details from the method were changed. | The transformation protocol from 4/7/13 was followed, no details from the method were changed. | ||
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Liquid cultures were made of... | Liquid cultures were made of... | ||
| - | - BBa_K592012, our magenta pigment | + | - [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], our magenta pigment |
| - | - BBa_K592010, our yellow pigment | + | - [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], our yellow pigment |
| - | - BBa_K322122, our cyan pigment | + | - [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], our cyan pigment |
== (Next day, results from transformation) == | == (Next day, results from transformation) == | ||
| - | BBa_K592004, blue light sensor - 94 colonies | + | [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor - 94 colonies |
| - | BBa_K608002, promoter and RBS - 112 colonies | + | [http://parts.igem.org/Part:BBa_K608002 BBa_K608002], promoter and RBS - 112 colonies |
| - | BBa_K592006, FixJ promoter - 73 colonies | + | [http://parts.igem.org/Part:BBa_K592006 BBa_K592006], FixJ promoter - 73 colonies |
| - | BBa_B0015, a terminator - 118 colonies | + | [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator - 118 colonies |
Our BBa_B0034, an RBS, had no colonies as we hadn't noted that this part was stored on pSB1A2, so plating it onto a chloramphenicol plate killed all of the colonies. A retry of this transformation will be undertaken tomorrow. | Our BBa_B0034, an RBS, had no colonies as we hadn't noted that this part was stored on pSB1A2, so plating it onto a chloramphenicol plate killed all of the colonies. A retry of this transformation will be undertaken tomorrow. | ||
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| + | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
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| + | </div> | ||
| + | </div> | ||
| + | {{:Team:Exeter/Template/Footer}} | ||
Latest revision as of 19:49, 2 October 2013
Our requested DNA has arrived from iGEM!
The Parts sent over were...
- Magenta pigment coding ([http://parts.igem.org/Part:BBa_K592012 BBa_K592012], pSB1C3)
- Green light sensor ([http://parts.igem.org/Part:BBa_K592001 BBa_K592001], pSB1C3)
- Yellow pigment coding ([http://parts.igem.org/Part:BBa_K592010 BBa_K592010], pSB1C3)
- Cyan pigment coding ([http://parts.igem.org/Part:BBa_K322122 BBa_K322122], pSB1C3)
- FixJ intermediate protein coding ([http://parts.igem.org/Part:BBa_K592005 BBa_K592005], pSB1C3)
We also requested some extra parts from the Registry
- 2 Genome Integration Kits ([http://parts.igem.org/Part:BBa_K510000 BBa_K510000] and [http://parts.igem.org/Part:BBa_K510012 BBa_K510012]). We know we want to genome integrate some of our Parts at some point...
- Alternative cyan pigments ([http://parts.igem.org/Part:BBa_K592011 BBa_K592011] just codes for the pigment but has no promoter, RBS or terminator. [http://parts.igem.org/Part:BBa_K864404 BBa_K864404] codes for the same pigment, but has a promoter and RBS. [http://parts.igem.org/Part:BBa_K592022 BBa_K592022] also has the same coding region and RBS, but an alternative promoter)
Afternoon
Transforming the BioBricks we will be utilising in the blue light module
We are transforming...
- [http://parts.igem.org/Part:BBa_K608002 BBa_K608002], codes for a promoter and RBS
- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], our blue light sensor
- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator
- [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 BBa_B0034], an RBS
- [http://parts.igem.org/Part:BBa_K592006 K592006], the promoter that is activated by FixJ
We already have the following Parts as LB stab plates from the iGEM registry, so a transformation of these Parts is required:
- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], codes for our intermediate protein, FixJ
- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], our yellow pigment
The transformation protocol from 4/7/13 was followed, no details from the method were changed.
Sequencing
We also sent our Cph8 plasmids off for sequencing.
Liquid cultures
Liquid cultures were made of...
- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], our magenta pigment
- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], our yellow pigment
- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], our cyan pigment
(Next day, results from transformation)
[http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor - 94 colonies
[http://parts.igem.org/Part:BBa_K608002 BBa_K608002], promoter and RBS - 112 colonies
[http://parts.igem.org/Part:BBa_K592006 BBa_K592006], FixJ promoter - 73 colonies
[http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator - 118 colonies
Our BBa_B0034, an RBS, had no colonies as we hadn't noted that this part was stored on pSB1A2, so plating it onto a chloramphenicol plate killed all of the colonies. A retry of this transformation will be undertaken tomorrow.
Take me back to the notebook.
With many thanks to our sponsors:
