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Revision as of 17:05, 27 October 2013

Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies from the previous experiment.

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current17:07, 27 October 2013Thumbnail for version as of 17:07, 27 October 2013725×351 (89 KB)Pk197 (Talk | contribs)
17:05, 27 October 2013Thumbnail for version as of 17:05, 27 October 2013742×375 (93 KB)Pk197 (Talk | contribs)
17:00, 27 October 2013Thumbnail for version as of 17:00, 27 October 2013763×401 (99 KB)Pk197 (Talk | contribs)
19:01, 27 September 2013Thumbnail for version as of 19:01, 27 September 2013800×446 (115 KB)Raafekie (Talk | contribs) (Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% A)

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