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From 2013.igem.org

1>Absorb pluxRI2 CM+ from the solution, next, dilute it into 30ml with five kinds of concentrations, 1:10, 1:20, 1:30, 1:40, and 1:50, then inject 22ul CM into it.

2> Detect the luminosity of the bacteria using the spectrometer.
Fetch the bacteria from the incubator every half an hour, and absorb 3ml to detect the luminosity.