"
Index.Transformation
From 2013.igem.org
(Difference between revisions)
Donna (Talk | contribs)
(Created page with "<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> Mix the two component...")
Newer edit →
(Created page with "<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> Mix the two component...")
Newer edit →
Revision as of 18:15, 26 September 2013
1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water.
Mix the two components and put the EP tube in the icy water for half an hour.
Next put them in the 42℃ water for 90 secs.
Inject the LB solution into the EP tube and culture them at least 40 mins.
Paste them in the plate.
