Index.Transformation

From 2013.igem.org

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(Created page with "<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> Mix the two component...")
 
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<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br>
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<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular.Then refresh the competent cells in the 0℃ icy water. <br>
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Mix the two components and put the EP tube in the icy water for half an hour.<br>
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3.Mix the two components and put the EP tube in the icy water for half an hour.<br>
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Next put them in the 42℃ water for 90 secs.<br>
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3.Next put them in the 42℃ water for 90 secs.<br>
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Inject the LB solution into the EP tube and culture them at least 40 mins.<br>
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4.Inject the LB solution into the EP tube and culture them at least 40 mins.<br>
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Paste them in the plate.
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5.Paste them in the plate.

Latest revision as of 18:17, 26 September 2013

1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular.Then refresh the competent cells in the 0℃ icy water.
3.Mix the two components and put the EP tube in the icy water for half an hour.
3.Next put them in the 42℃ water for 90 secs.
4.Inject the LB solution into the EP tube and culture them at least 40 mins.
5.Paste them in the plate.