Index.Transformation
From 2013.igem.org
1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water.
Mix the two components and put the EP tube in the icy water for half an hour.
Next put them in the 42℃ water for 90 secs.
Inject the LB solution into the EP tube and culture them at least 40 mins.
Paste them in the plate.