Saturday, 06 Jul 2013

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7/06/13 Primer Design Part II

Designed primers to isolate non-coding and coding sequence of kerA and add prefix and suffix

forward primer + xbaI site:

5' TATCTAGAGGTCTATTCATACTTTCGAA 3'

reverse primer + speI site (reverse complement):

5' ATTGATCATTGGACAACCTTCATCAG 3'

We still need to design additional primers that will include the secretion signal peptide (in case it needs to be secreted out of B. subtilis) and kerA (From start to stop codon).
These primers should include igem biobrick prefix and his tag (before stop codon) + suffix.

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