User contributions
From 2013.igem.org
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- 00:51, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3) (top)
- 00:48, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 (→2 - Ligation of pSB1C3 and Pndh*) (top)
- 00:47, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 (→2 - Ligation of pSB1C3 and Pndh*)
- 00:45, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 (→1 - Digestion of pSB1C3 plasmid and PCR products : Pndh* by EcoRI/ PstI)
- 00:42, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/28 (→Lab work)
- 00:36, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/28 (→Lab work)
- 00:31, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/27 (→Lab work) (top)
- 00:29, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/26 (→Lab work) (top)
- 00:25, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/25 (→Labwork) (top)
- 00:22, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/24 (→Lab work) (top)
- 00:16, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/21 (→Lab work) (top)
- 00:04, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/20 (→Notebook : June 20) (top)
- 00:02, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/19 (→Notebook : June 19) (top)
- 00:01, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/18 (→Notebook : June 18) (top)
- 23:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 (→Saturday 20th April :) (top)
- 23:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 (→Week end Brainstorming : 20&21 April 2013)
- 23:53, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 (→Notebook : April 20)
- 17:47, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→1 - Results of liquid culture of Pfnr-RBS-Amil CP-Term in pSB1C3 in aerobic and anaerobic conditions)
- 17:44, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/31 (→1 - Results of culture of Pfnr with RBS-LacZ-Term in PSB1K3, Pfnr with RBS-LacZ-Term in PSB1C3 and NarK with RBS-LacZ-term in PSB3K3 in aerobic conditions)
- 17:42, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→7 - Electrophoresis of PCR Colony of Pndh* with RBS_AmilCP-Term in DH5α strain)
- 17:42, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→6 - Transformation of ligation of Pfnr with RBS-LacZ-Term in pSB3K3 and PnarK with RBS-LacZ-Term in pSB3K3 in DH5α strain)
- 17:41, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→7 - Electrophoresis of PCR Colony of Pfnr with RBS_AmilCP-Term in DH5α strain)
- 17:39, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→6 - Transformation of ligation of Pfnr with RBS-LacZ-Term in pSB3K3 and PnarK with RBS-LacZ-Term in pSB3K3 in DH5α strain)
- 17:37, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→Notebook : August 30)
- 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 (→1 - Culture of BBa_I732017, BBa_K592009)
- 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 (→2 - Culture of BBa_K1155000)
- 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3)
- 17:03, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3)
- 17:01, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→7 - Electrophoresis to check the digestion of BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI/PstI and plasmids already digested by SpeI and after digested by PstI)
- 16:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→2 - PCR Colony of strain DH5α containing plasmid pSB1C3 with Pndh* and RBS_AmilCP-Term)
- 16:53, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 (→6 - Electrophoresis to check the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI and EcoRI/SpeI)
- 16:50, 4 October 2013 (diff | hist) Team:Paris Saclay/Primers (→Primers)
- 16:49, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→2 - Colony PCR of Pfnr with RBS_AmilCP-Term in DH5α)
- 16:45, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Purification of colonies: NirB with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-AmilCP-Term in pSB1C3 by streaking colonies in aerobic or anaerobic conditions)
- 16:43, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Purification of colonies: NirB with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-Amil CP-Term in pSB1C3 by streaking colonies in aerobic or anaerobic conditions)
- 16:16, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 (→1 - Results of liquid culture of Pfnr-RBS-Amil CP-Term in pSB1C3 in aerobic and anaerobic conditions)
- 16:03, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Colony PCR of FNR, RBS-FNR and RBS-BphR2 in DH5α strain)
- 16:01, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/September/12 (→1 - Extraction of BBa_K1155000, BBa_K1155001, BBa_K1155002, BBa_K1155003, BBa_K1155004) (top)
- 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Colony PCR of FNR, RBS-FNR and RBS-BphR2 in DH5α)
- 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→3 - Gel purification of the digestion of BBa_J04450 by PstI/SpeI)
- 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→2 - Electrophoresis to check the digestion of BBa_J04450 by EcoRI/PstI)
- 15:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Digestion of BBa_J04450 by EcoRI/PstI)
- 15:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→6 - Electrophoresis to check the gel purification of the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
- 15:56, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→5 - Gel purification of the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
- 15:56, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→4 - Electrophoresis to check the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
- 15:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→4 - Electrophoresis to check the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
- 15:51, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→1 - Purification of colony transformed with ligation : NirB with RBS-LacZ-Term in PSB1C3, Pfnr with RBS-Amil CP-Term in PSB1C3 by streaking in aerobic or anaerobic conditions)
- 15:49, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 (→3 - Digestion of BBa_K1155000, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
- 15:48, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/28 (→2 - Transformation of FNR, RBS-FNR and RBS-BphR2 in DH5α)
- 15:48, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 (→1 - Gibson assembly)
- 15:47, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/28 (→3 - Gibson assembly)
- 15:43, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/28 (/* 1 - Purification of colony transformed with ligation : NirB with RBS-LacZ-Term in pSB1C3, NirB with RBS-Amil CP-Term in pSB1C3, Pfnr with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-Amil CP-Term in pSB1C3 by streaking in aerobic or anaerobic conditions)
- 15:43, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/28 (/* 1 - Purification of colony transformed with ligation : NirB with RBS-LacZ-Term in pSB1C3, NirB with RBS-Amil CP-Term in pSB1C3, Pfnr with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-Amil CP-Term in pSB1C3 by streaking in aerobic or anaerobic conditions)
- 15:41, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/27 (→3 - Electrophoresis of the digestion of PSB1C3 by DnpI)
- 15:38, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/27 (→3 - Streak of NirB with RBS-Amil CP-Term in PSB1C3, Pfnr with RBS-LacZ-Term in PSB1C3 and Pfnr with RBS-Amil CP-Term in PSB1C3 to purify them)
- 15:36, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/26 (→2 - Denaturation of SpeI/PstI used for the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006)
- 15:34, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 (→2 - Electrophoresis of PCR product : BphR2 Part I)
- 15:34, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/22 (→1 - PCR of BphR2 Part I)
- 15:33, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/19 (→Meeting with Evry)
- 15:32, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 (→3 - PCR of BphR2 Part I)
- 15:31, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 (→10 - Glycerol stock of MG1655Z1 Δfnr::Km)
- 15:30, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 (→10 - Glycerol stock of MG1655Z1 Δfnr::Km)
- 15:28, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/9 (→1 - Tranduction of Km in MG1655Z1) (top)
- 15:27, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/9 (→1 - Tranduction of Km in MG1655Z1)
- 15:24, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/8 (→1 - Tranduction of Km in MG1655Z1)
- 15:18, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 (→4 - PCR of BphR2 Part I)
- 15:18, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/13 (→5 - Gel purification of PCR product : FNR Part I, FNR Part II, RBS-FNR Part I, BphR2 Part II, RBS-BphR2 Part I)
- 15:06, 4 October 2013 (diff | hist) Team:Paris Saclay/transduction (→Protocol : Transduction) (top)
- 15:01, 4 October 2013 (diff | hist) Team:Paris Saclay/transduction (→Protocol : Transduction)
- 14:59, 4 October 2013 (diff | hist) Team:Paris Saclay/digestion ligation (→Digestion and Ligation) (top)
- 14:46, 4 October 2013 (diff | hist) Team:Paris Saclay/digestion ligation (→Digestion and Ligation)
- 14:33, 4 October 2013 (diff | hist) Team:Paris Saclay/digestion ligation (→Digestion and Ligation)
- 14:23, 4 October 2013 (diff | hist) Team:Paris Saclay/ethanol (→Ethanol precipitation) (top)
- 14:12, 4 October 2013 (diff | hist) Team:Paris Saclay/ethanol (→Ethanol precipitation)
- 13:44, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis) (top)
- 13:42, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:40, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:39, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:22, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:18, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:18, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:17, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Gel Electrophoresis)
- 13:16, 4 October 2013 (diff | hist) Team:Paris Saclay/gel (→Electrophoresis on gel)
- 12:58, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly) (top)
- 12:57, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:57, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:57, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:56, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:55, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:53, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:50, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:49, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:49, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:48, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:48, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:41, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:40, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:37, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:33, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
- 12:32, 4 October 2013 (diff | hist) Team:Paris Saclay/gibson (→Gibson Assembly)
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