User contributions
From 2013.igem.org
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- 20:08, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/15 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Analysis of the sequencing resutls of the three promoters (sensing-module)''' <BR> The sequencing results were rather promising exce...")
- 20:04, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/14 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Making Buffers for the functional Assays''' <BR> In order to test the sensing module I wanted to let the bacteria grow in media with...")
- 19:59, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/13 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Innoculation of Bacteria with our constructs''' <BR> In order to characterize both the sensing and the effector plasmid I innoculate...")
- 19:57, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/12 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Test PCR for the constitutive promoter and the GelE insert''' <BR> I did a PCR of the MiniPrep and it showed that the plasmid did in...")
- 19:55, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/11 September 2013
- 19:53, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 19:52, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/10 September 2013
- 19:50, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/11 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of hya-construct (sensing), MMP2-construct (effector) and MMP9-construct (effector)''' <BR> .After the Minipreps of the innocuat...")
- 19:47, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/10 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''DpnI digest of the iGEM promoter and the GelE Backbone''' <BR> I digested the newly amplified and purified PCR products. The concent...")
- 19:39, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/9 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gibson Assembly of the hya-construct (sensing), the MMP2+GFP construct and the MMP9+GFP construct (effector)''' <BR> I did a Gibson ...")
- 19:32, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/8 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP9 insert and GelE insert''' <BR> I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave...")
- 19:15, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/7 September 2013
- 19:02, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/7 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Purification of PCR products of the three sensing constructs''' <BR> I purified the PCR products from the previous Day and the conce...")
- 18:54, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/6 September 2013
- 18:54, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/6 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR> I did a 50ul PCR reaction of the Backbone and...")
- 18:51, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/5 September 2013
- 18:48, 30 September 2013 (diff | hist) N Team:EPF Lausanne/Calendar/5 September 2013 (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM''' <BR> We used the same program as the day before in ...")
- 18:45, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 18:43, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
- 18:42, 30 September 2013 (diff | hist) Team:EPF Lausanne/Calendar/4 September 2013
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