http://2013.igem.org/wiki/index.php?title=Special:Contributions/0sec8&feed=atom&limit=50&target=0sec8&year=&month=2013.igem.org - User contributions [en]2024-03-29T13:40:21ZFrom 2013.igem.orgMediaWiki 1.16.5http://2013.igem.org/Team:Queens_Canada/TeamTeam:Queens Canada/Team2013-09-28T00:17:32Z<p>0sec8: </p>
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<!-----MEET THE TEAM------><br />
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QGEM 2013 PRESENTS:<br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
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<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
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<span class="black">Here's a little bit </span><span class="rose">about us</span><br />
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<div class="span3 offset1 text-left"><br />
<div class="content" style="text-align:justify;">Queen's University is located in Kingston, Ontario, Canada's first capital in 1841. Their iGEM team is composed of dedicated students who have devoted their summer to working with synthetic biology, toiling away in labs, promoting the field to others in outreach, and having a blast along the way. Our project this year initially began as an idea to remove foot odour. It eventually evolved to a much broader idea involving mosquitoes, malaria, and smelly feet! To find out more about what we've been up to, click over to our Project and Parts pages!</div><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black"> Meet the </span><span class="rose">Team</div></div><br />
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<div class="name"><strong>HENRY BARRON</strong></div><br />
Henry is a third-year student studying Life Sciences. He dreams of a future that is inspiring and exciting. He also loves art but can't draw or paint. These two things have led him to synthetic biology.<br />
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<div class="name"><strong>JAMES KIM</strong></div><br />
James is a second-year student studying Life Sciences. As a scholar, gentleman as well as a boxer, you can likely find him holding doors open for damsels, pondering the mysteries of the universe, and performing spartan pushups (yes, they are as difficult as they sound).<br />
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<div class="name"><strong>MACKENZIE JAMIESON</strong></div><br />
Mack is a fourth-year Life Sciences student. His spirit animal is the dragon because his smouldering eyes could probably burn anyone to a crisp. His commitment to toned biceps is matched only by his love for synthetic biology, the canvas upon which he paints with powerful strokes of genius. </div><br />
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<div class="name"><strong>SYLVESTER CHIANG</strong></div><br />
Sylvester is a third-year student studying Biomedical Computing. He is aptly described as a combination of his predecessors, Sylvester the cat and Sylvester Stallone, an individual of persistence and impressive muscular structure.<br />
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<div class="name"><strong>CINDY ZHU</strong></div><br />
Cindy is a fourth-year Life Sciences and Psychology student. She is excited to join iGEM due to the infinite possibilities of synthetic biology, and to contribute to both labwork and creative projects. In her spare time, she enjoys reading Oliver Sacks, Youtubing Broadway musicals, and sangria.<br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Dylan.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Dylan_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Dylan.png'"/> <br />
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<div class="name"><strong>DYLAN PIETTE</strong></div><br />
Dylan entering his fourth-year in the Biochemistry program. He really enjoys building orphanages in third-world countries, raising money for the donation of livestock and when it all gets too much, Dylan will kick back in his lawn chair and work on his children's novel.<br />
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<div class="name"><strong>SIMON HUANG</strong></div><br />
Simon is a fourth-year Life Science student. Much like Charlie Sheen, it is very difficult to find him not winning. When he isn’t exploring the wonders of synthetic biology, he secretly trains to be a Tetris pro.<br />
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<div class="name"><strong>MITANGI PAREKH</strong></div><br />
Mitangi Parekh is going into her fifth-year of Biology and English and is ridiculously excited to be back with QGEM for yet another summer. Her passions include writing and drinking copious amounts of iced coffee. She also has a special bond with huskies; in a remote village in Siberia, she is known as The Husky Whisperer. <br />
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<div class="name"><strong>TONY ZHU</strong></div><br />
Tony is going into third year of Life Sciences at Queen's. He's allergic to mangoes but he eats them anyways.<br />
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<div class="name"><strong>STEPHANIE CHAN</strong></div><br />
Stephanie blends in with the high schoolers, but in reality she is a third-year Life Sciences student. When not doing plasmid preps or running PCR gels, Steph can be found in her natural habitat, Stauffer, battling her MCAT books. She is also a keen curator of cute cat captions.<br />
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<div class="name"><strong>ANDY SONG</strong></div><br />
Andy is entering his fourth-year as a Life Sciences major and hails from Nova Scotia, a land famous for Keith's and copious amounts of salt water. He enjoys playing sports, hanging out with friends, and playing Settlers of Catan. His academic interests include GI cell physiology, saccade movements, and of course synthetic biology.<br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Kyle.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Kyle_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Kyle.png'"/> <br />
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<div class="name"><strong>KYLE SUNDERLAND</strong></div><br />
Kyle is entering his third-year in the Biomedical Computing program at Queen's. With a strong interest in Neuroscience and prosthetic limbs, he figured iGEM would be a great way to combine his interests of biology and computing together. He hasn't looked back since. <br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Laura.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Laura.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Laura.png'"/><br />
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<div class="name"><strong>LAURA CHU</strong></div><br />
Laura is entering her third year of Life Sciences. She loves cooking, reading, and watching a good old Leafs game. She loves heavy metal music and has recently developed an addiction to Benedict Cumberbatch's cheekbones. She can often be found daydreaming about her next meal.<br />
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<div class="name"><strong>MADDY TONG</strong></div> <br />
Maddy is entering her third year in the Life Sciences program. Her days are often spent on the computer playing video games, working with Photoshop, and browsing aimlessly on websites. She also enjoys cooking, eating, and pipetting things in the lab.<br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">QGEM </span><span class="rose">Advisors</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid white"><br />
<div class="offset1 span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/chin-sang.png" style="width:250px;"/><br />
<a href="http://post.queensu.ca/~chinsang/"><h4>Dr. Ian Chin-Sang</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/bendena.png" style="width:250px;"/><br />
<a href="http://www.queensu.ca/biology/people/faculty/bendena.html"><h4>Dr. William Bendena</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/ko.png" style="width:250px;"/><br />
<a href="http://post.queensu.ca/~kok/"><h4>Dr. Kenton Ko</h4></a><br />
</div><br />
</div><br />
<div class="row-fluid white"><br />
<div class="offset1 span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/allingham.png" style="width:250px;"/><br />
<a href="http://www.chairs-chaires.gc.ca/chairholders-titulaires/profile-eng.aspx?profileID=2153"><h4>Dr. John Allingham</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/zechel.png" style="width:250px;"/><br />
<a href="http://www.chem.queensu.ca/people/faculty/Zechel/Group%20Members.htm"><h4>Dr. David Zechel</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/petkovich.png" style="width:250px;"/><br />
<a href="http://qcri.queensu.ca/Petkovich.html"><h4>Dr. Martin Petkovich</h4></a><br />
</div><br />
</div><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/TeamTeam:Queens Canada/Team2013-09-28T00:00:47Z<p>0sec8: </p>
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<div class="header-bg"><br />
<div class="header-text"><br />
QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/f/ff/Team_qgem.png" style="height:110px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
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<br />
<div class="menuc" style="font-size:1.3em;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="tidal-wave"><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">Here's a little bit </span><span class="rose">about us</span><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="span3 offset1 text-left"><br />
<div class="content" style="text-align:justify;">Queen's University is located in Kingston, Ontario, a small city located on the eastern side of Canada. Their iGEM team is composed of dedicated students who have devoted their summer to working with synthetic biology, toiling away in labs, and, of course, having a blast. Our project this year initially began as an idea to remove foot odour. It eventually evolved to a much broader idea involving mosquitoes, malaria, and smelly feet! To find out more about what we've been up to, keep reading!</div><br />
</div><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black"> Meet the </span><span class="rose">Team</div></div><br />
<div class="row-fluid"><br />
<div class="span5 offset1 red"><br />
<div class="holder"><br />
<div class="left"><br />
<div class="photo-left"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/Henry.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Henry_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Henry.png'"/><br />
</div><br />
<div class="name"><strong>HENRY BARRON</strong></div><br />
<div style="text-align:justify;">Henry is a third-year student studying Life Sciences. He dreams of a future that is inspiring and exciting. He also loves art but can't draw or paint. These two things have led him to synthetic biology.</div><br />
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<div class="name"><strong>JAMES KIM</strong></div><br />
<div style="text-align:justify;">James is a second-year student studying Life Sciences. As a scholar, gentleman as well as a boxer, you can likely find him holding doors open for damsels, pondering the mysteries of the universe, and performing spartan pushups (yes, they are as difficult as they sound).</div><br />
</div><br />
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<div class="name"><strong>MACKENZIE JAMIESON</strong></div><br />
<div style="text-align:justify;">Mack is a fourth-year Life Sciences student. His spirit animal is the dragon because his smouldering eyes could probably burn anyone to a crisp. His commitment to toned biceps is matched only by his love for synthetic biology, the canvas upon which he paints with powerful strokes of genius.</div> </div><br />
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<div class="right"><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Syl.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Syl_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Syl.png'"/> <br />
</div><br />
<div class="name"><strong>SYLVESTER CHIANG</strong></div><br />
<div style="text-align:justify;">Sylvester is a third-year student studying Biomedical Computing. He is aptly described as a combination of his predecessors, Sylvester the cat and Sylvester Stallone, an individual of persistence and impressive muscular structure. </div><br />
</div><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Cindy.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Cindy_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Cindy.png'"/><br />
</div><br />
<div class="name"><strong>CINDY ZHU</strong></div><br />
<div style="text-align:justify;">Cindy is a fourth-year Life Sciences and Psychology student. She is excited to join iGEM due to the infinite possibilities of synthetic biology, and to contribute to both labwork and creative projects. In her spare time, she enjoys reading Oliver Sacks, Youtubing Broadway musicals, and sangria. </div><br />
</div><br />
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<div class="span5 yellow"><br />
<div class="holder"><br />
<div class="right"><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Dylan.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Dylan_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Dylan.png'"/> <br />
</div><br />
<div class="name"><strong>DYLAN PIETTE</strong></div><br />
<div style="text-align:justify;">Dylan entering his fourth-year in the Biochemistry program. He really enjoys building orphanages in third-world countries, raising money for the donation of livestock and when it all gets too much, Dylan will kick back in his lawn chair and work on his children's novel.</div><br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="span5 offset1 red"><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Simon.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Simon_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Simon.png'"/><br />
</div><br />
<div class="name"><strong>SIMON HUANG</strong></div><br />
<div style="text-align:justify;">Simon is a fourth-year Life Science student. Much like Charlie Sheen, it is very difficult to find him not winning. When he isn’t exploring the wonders of synthetic biology, he secretly trains to be a Tetris pro. </div><br />
</div><br />
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<div class="span5 blue"><br />
<div class="holder"><br />
<div class="right"><br />
<div class="photo-left"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/Mitangi.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Mitangi_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Mitangi.png'"/> <br />
</div><br />
<div class="name"><strong>MITANGI PAREKH</strong></div><br />
<div style="text-align:justify;">Mitangi Parekh is going into her fifth-year of Biology and English and is ridiculously excited to be back with QGEM for yet another summer. Her passions include writing and drinking copious amounts of iced coffee. She also has a special bond with huskies; in a remote village in Siberia, she is known as The Husky Whisperer. </div><br />
</div><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Tony.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Tony_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Tony.png'"/><br />
</div><br />
<div class="name"><strong>TONY ZHU</strong></div><br />
<div style="text-align:justify;">Tony is going into third year of Life Sciences at Queen's. He's allergic to mangoes but he eats them anyways.</div><br />
</div><br />
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</div><br />
<div class="name"><strong>STEPHANIE CHAN</strong></div><br />
<div style="text-align:justify;">Stephanie blends in with the high schoolers, but in reality she is a third-year Life Sciences student. When not doing plasmid preps or running PCR gels, Steph can be found in her natural habitat, Stauffer, battling her MCAT books. She is also a keen curator of cute cat captions. </div><br />
</div><br />
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<div class="row-fluid"><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Andy.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Andy_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Andy.png'"/><br />
</div><br />
<div class="name"><strong>ANDY SONG</strong></div><br />
<div style="text-align:justify;"> Andy is entering his fourth-year as a Life Sciences major and hails from Nova Scotia, a land famous for Keith's and copious amounts of salt water. He enjoys playing sports, hanging out with friends, and playing Settlers of Catan. His academic interests include GI cell physiology, saccade movements, and of course synthetic biology. </div><br />
</div><br />
</div><br />
</div><br />
<div class="span5 yellow"><br />
<div class="holder"><br />
<div class="right"><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Kyle.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Kyle_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Kyle.png'"/> <br />
</div><br />
<div class="name"><strong>KYLE SUNDERLAND</strong></div><br />
<div style="text-align:justify;">Kyle is entering his third-year in the Biomedical Computing program at Queen's. With a strong interest in Neuroscience and prosthetic limbs, he figured iGEM would be a great way to combine his interests of biology and computing together. He hasn't looked back since. </div><br />
</div><br />
</div><br />
</div><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Laura.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Laura.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/Laura.png'"/><br />
</div><br />
<div class="name"><strong>LAURA CHU</strong></div><br />
<div style="text-align:justify;">Laura is entering her third year of Life Sciences. She loves cooking, reading, and watching a good old Leafs game. She loves heavy metal music and has recently developed an addiction to Benedict Cumberbatch's cheekbones. She can often be found daydreaming about her next meal. </div><br />
</div><br />
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<img src="https://dl.dropboxusercontent.com/u/125588631/Maddy.png" onmouseover="this.src='https://dl.dropboxusercontent.com/u/125588631/Maddy_whim.png'" onmouseout="this.src='https://dl.dropboxusercontent.com/u/125588631/maddy.png'"/><br />
</div> <br />
</div><br />
<div class="name"><strong>MADDY TONG</strong></div> <br />
<div style="text-align:justify;">Maddy is entering her third year in the Life Sciences program. Her days are often spent on the computer playing video games, working with Photoshop, and browsing aimlessly on websites. She also enjoys cooking, eating, and pipetting things in the lab.</div><br />
</div><br />
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</div><br />
<br />
<div class="row-fluid white"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">QGEM </span><span class="rose">Advisors</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid white"><br />
<div class="offset1 span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/chin-sang.png" style="width:250px;"/><br />
<a href="http://post.queensu.ca/~chinsang/"><h4>Dr. Ian Chin-Sang</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/bendena.png" style="width:250px;"/><br />
<a href="http://www.queensu.ca/biology/people/faculty/bendena.html"><h4>Dr. William Bendena</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/ko.png" style="width:250px;"/><br />
<a href="http://post.queensu.ca/~kok/"><h4>Dr. Kenton Ko</h4></a><br />
</div><br />
</div><br />
<div class="row-fluid white"><br />
<div class="offset1 span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/allingham.png" style="width:250px;"/><br />
<a href="http://www.chairs-chaires.gc.ca/chairholders-titulaires/profile-eng.aspx?profileID=2153"><h4>Dr. John Allingham</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/zechel.png" style="width:250px;"/><br />
<a href="http://www.chem.queensu.ca/people/faculty/Zechel/Group%20Members.htm"><h4>Dr. David Zechel</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/petkovich.png" style="width:250px;"/><br />
<a href="http://qcri.queensu.ca/Petkovich.html"><h4>Dr. Martin Petkovich</h4></a><br />
</div><br />
</div><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/TeamTeam:Queens Canada/Team2013-09-27T23:53:23Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<img src="https://static.igem.org/mediawiki/2013/f/ff/Team_qgem.png" style="height:110px;" /><br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<span class="black">Here's a little bit </span><span class="rose">about us</span><br />
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<div class="content" style="text-align:justify;">Queen's University is located in Kingston, Ontario, a small city located on the eastern side of Canada. Their iGEM team is composed of dedicated students who have devoted their summer to working with synthetic biology, toiling away in labs, and, of course, having a blast. Our project this year initially began as an idea to remove foot odour. It eventually evolved to a much broader idea involving mosquitoes, malaria, and smelly feet! To find out more about what we've been up to, keep reading!</div><br />
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<div class="name"><strong>HENRY BARRON</strong></div><br />
Henry is a third-year student studying Life Sciences. He dreams of a future that is inspiring and exciting. He also loves art but can't draw or paint. These two things have led him to synthetic biology.<br />
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<div class="name"><strong>JAMES KIM</strong></div><br />
James is a second-year student studying Life Sciences. As a scholar, gentleman as well as a boxer, you can likely find him holding doors open for damsels, pondering the mysteries of the universe, and performing spartan pushups (yes, they are as difficult as they sound).<br />
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<div class="name"><strong>MACKENZIE JAMIESON</strong></div><br />
Mack is a fourth-year Life Sciences student. His spirit animal is the dragon because his smouldering eyes could probably burn anyone to a crisp. His commitment to toned biceps is matched only by his love for synthetic biology, the canvas upon which he paints with powerful strokes of genius. </div><br />
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<div class="name"><strong>SYLVESTER CHIANG</strong></div><br />
Sylvester is a third-year student studying Biomedical Computing. He is aptly described as a combination of his predecessors, Sylvester the cat and Sylvester Stallone, an individual of persistence and impressive muscular structure. <br />
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<div class="name"><strong>CINDY ZHU</strong></div><br />
Cindy is a fourth-year Life Sciences and Psychology student. She is excited to join iGEM due to the infinite possibilities of synthetic biology, and to contribute to both labwork and creative projects. In her spare time, she enjoys reading Oliver Sacks, Youtubing Broadway musicals, and sangria.<br />
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<div class="name"><strong>DYLAN PIETTE</strong></div><br />
Dylan entering his fourth-year in the Biochemistry program. He really enjoys building orphanages in third-world countries, raising money for the donation of livestock and when it all gets too much, Dylan will kick back in his lawn chair and work on his children's novel.<br />
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<div class="name"><strong>SIMON HUANG</strong></div><br />
Simon is a fourth-year Life Science student. Much like Charlie Sheen, it is very difficult to find him not winning. When he isn’t exploring the wonders of synthetic biology, he secretly trains to be a Tetris pro. <br />
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<div class="name"><strong>MITANGI PAREKH</strong></div><br />
Mitangi Parekh is going into her fifth-year of Biology and English and is ridiculously excited to be back with QGEM for yet another summer. Her passions include writing and drinking copious amounts of iced coffee. She also has a special bond with huskies; in a remote village in Siberia, she is known as The Husky Whisperer.<br />
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<div class="name"><strong>TONY ZHU</strong></div><br />
Tony is going into third year of Life Sciences at Queen's. He's allergic to mangoes but he eats them anyways.<br />
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<div class="name"><strong>STEPHANIE CHAN</strong></div><br />
Stephanie blends in with the high schoolers, but in reality she is a third-year Life Sciences student. When not doing plasmid preps or running PCR gels, Steph can be found in her natural habitat, Stauffer, battling her MCAT books. She is also a keen curator of cute cat captions. <br />
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<div class="name"><strong>ANDY SONG</strong></div><br />
Andy is entering his fourth-year as a Life Sciences major and hails from Nova Scotia, a land famous for Keith's and copious amounts of salt water. He enjoys playing sports, hanging out with friends, and playing Settlers of Catan. His academic interests include GI cell physiology, saccade movements, and of course synthetic biology. <br />
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<div class="name"><strong>KYLE SUNDERLAND</strong></div><br />
Kyle is entering his third-year in the Biomedical Computing program at Queen's. With a strong interest in Neuroscience and prosthetic limbs, he figured iGEM would be a great way to combine his interests of biology and computing together. He hasn't looked back since. <br />
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<div class="name"><strong>LAURA CHU</strong></div><br />
Laura is entering her third year of Life Sciences. She loves cooking, reading, and watching a good old Leafs game. She loves heavy metal music and has recently developed an addiction to Benedict Cumberbatch's cheekbones. She can often be found daydreaming about her next meal. <br />
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<div class="name"><strong>MADDY TONG</strong></div> <br />
Maddy is entering her third year in the Life Sciences program. Her days are often spent on the computer playing video games, working with Photoshop, and browsing aimlessly on websites. She also enjoys cooking, eating, and pipetting things in the lab.<br />
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<span class="black">QGEM </span><span class="rose">Advisors</span><br />
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<div class="offset1 span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/chin-sang.png" style="width:250px;"/><br />
<a href="http://post.queensu.ca/~chinsang/"><h4>Dr. Ian Chin-Sang</h4></a><br />
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<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/bendena.png" style="width:250px;"/><br />
<a href="http://www.queensu.ca/biology/people/faculty/bendena.html"><h4>Dr. William Bendena</h4></a><br />
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<a href="http://post.queensu.ca/~kok/"><h4>Dr. Kenton Ko</h4></a><br />
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<a href="http://www.chairs-chaires.gc.ca/chairholders-titulaires/profile-eng.aspx?profileID=2153"><h4>Dr. John Allingham</h4></a><br />
</div><br />
<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/zechel.png" style="width:250px;"/><br />
<a href="http://www.chem.queensu.ca/people/faculty/Zechel/Group%20Members.htm"><h4>Dr. David Zechel</h4></a><br />
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<div class="span3 portrait"><img src="https://dl.dropboxusercontent.com/u/125588631/petkovich.png" style="width:250px;"/><br />
<a href="http://qcri.queensu.ca/Petkovich.html"><h4>Dr. Martin Petkovich</h4></a><br />
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QGEM 2013 PRESENTS:<br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
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<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">QGEM 2013 </span><span class="black">Sponsors</span></div><br />
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Queen's iGEM Team would like to thank all of our generous sponsors!<br />
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<a href ="http://www.queensu.ca/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Queen's University</span></a><br />
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We graciously thank the Department of Chemical Engineering, the Department of Biomedical and Molecular Sciences, the Department of Life Sciences and Biochemistry, the Department of Arts and Science, and the Department of Biology for their generous support of our team! In addition, we would like to thank the Faculty of Engineering and Applied Science for their continued support. <br />
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<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg"/><br />
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<a href ="http://www.pipetpeoplestore.com/index.cfm/fuseaction/home.home/index.htm" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Calibrate Inc.</span></a><br />
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Calibrate, Inc. provides pipette calibration, repair, and documentation services to scientific laboratories across North America. The largest and oldest independent pipette calibration and repair service in North America, Calibrate is a leader in quality in the industry and is known for delivering top-notch work and excellent customer service. <br />
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<img style="height:150px;text-align:center;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg"/><br />
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<a href ="http://qubitsystems.com/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Qubit Systems Inc.</span></a><br />
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<div class="offset1 span7 content" style="text-align:justify;"><br />
Qubit Systems Inc. specializes in the design of instrumentation for research and teaching in the biological sciences, with a scope ranging from plant physiology to human respirometry. Their aim is to provide educational establishments, research institutes and companies with innovative, cost-effective equipment for research, teaching, and industrial applications. Qubit also manufactures and sells a broad range of gas analyzers, environmental sensors, instruments for aquatic biology, and the world's most sophisticated range of fluorescence imaging systems for chlorophyll, GFP and other biologically important compounds.<br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;margin-left:30px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.conocophillips.ca/EN/Pages/index.aspx" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">ConocoPhillips</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
ConocoPhillips is a multinational energy corporation that explores for, produces, transports and markets crude oil, natural gas, natural gas liquids, liquefied natural gas and bitumen on a worldwide basis - energy that plays a foundational role in enabling global economic development and human progress. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg"/><br />
</div><br />
</div><br />
<br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Interested in </span><span class="rose">Sponsoring?</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;"><br />
Here at QGEM, we have a lot of passion for what we do. The tragedy though is that passion isn't exchangeable for reagents, which is something that we also need an abundance of. If you would like to show your support for our cause and sponsor us, download the sponsorship package and see what exactly it entails!<br />
</div> <br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span12" style="text-align:center;"><br />
<a href ="https://dl.dropboxusercontent.com/u/125588631/QGEM-FINAL.pdf"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/download.png" style="height:200px; float:center;"/><br />
</a><br />
</div><br />
<div class="row-fluid"><br />
<div class="span12" style="vertical-align:middle;text-align:center;"><br />
<h4>Money can't buy us love but it can buy us lab equipment.</h4><br />
<h5>Click here to download the sponsorship package in pdf format! We promise it's nifty.</h5><br />
</div><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:40px;margin-bottom:40px;"><br />
If you have any questions or inquiries about the sponsorship package or potential benefits, don't hesitate to email our project manager Henry Barron at henry.barron@qgemteam.com. <br />
</div><br />
</div><br />
<br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/SponsorshipTeam:Queens Canada/Sponsorship2013-09-27T23:49:55Z<p>0sec8: </p>
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">QGEM 2013 </span><span class="black">Sponsors</span></div><br />
</div></div><br />
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Queen's iGEM Team would like to thank all of our generous sponsors!<br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.queensu.ca/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Queen's University</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
We graciously thank the Department of Chemical Engineering, the Department of Biomedical and Molecular Sciences, the Department of Life Sciences and Biochemistry, the Department of Arts and Science, and the Department of Biology for their generous support of our team! In addition, we would like to thank the Faculty of Engineering and Applied Science for their continued support. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.pipetpeoplestore.com/index.cfm/fuseaction/home.home/index.htm" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Calibrate Inc.</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
Calibrate, Inc. provides pipette calibration, repair, and documentation services to scientific laboratories across North America. The largest and oldest independent pipette calibration and repair service in North America, Calibrate is a leader in quality in the industry and is known for delivering top-notch work and excellent customer service. <br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://qubitsystems.com/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Qubit Systems Inc.</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
Qubit Systems Inc. specializes in the design of instrumentation for research and teaching in the biological sciences, with a scope ranging from plant physiology to human respirometry. Their aim is to provide educational establishments, research institutes and companies with innovative, cost-effective equipment for research, teaching, and industrial applications. Qubit also manufactures and sells a broad range of gas analyzers, environmental sensors, instruments for aquatic biology, and the world's most sophisticated range of fluorescence imaging systems for chlorophyll, GFP and other biologically important compounds.<br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;margin-left:30px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.conocophillips.ca/EN/Pages/index.aspx" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">ConocoPhillips</span></a><br />
</di></div><br />
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<div class="offset1 span7 content" style="text-align:justify;"><br />
ConocoPhillips is a multinational energy corporation that explores for, produces, transports and markets crude oil, natural gas, natural gas liquids, liquefied natural gas and bitumen on a worldwide basis - energy that plays a foundational role in enabling global economic development and human progress. <br />
</div><br />
<div class="span4"><br />
<img style="height:200px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Interested in </span><span class="rose">Sponsoring?</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;"><br />
Here at QGEM, we have a lot of passion for what we do. The tragedy though is that passion isn't exchangeable for reagents, which is something that we also need an abundance of. If you would like to show your support for our cause and sponsor us, download the sponsorship package and see what exactly it entails!<br />
</div> <br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span12" style="text-align:center;"><br />
<a href ="https://dl.dropboxusercontent.com/u/125588631/QGEM-FINAL.pdf"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/download.png" style="height:200px; float:center;"/><br />
</a><br />
</div><br />
<div class="row-fluid"><br />
<div class="span12" style="vertical-align:middle;text-align:center;"><br />
<h4>Money can't buy us love but it can buy us lab equipment.</h4><br />
<h5>Click here to download the sponsorship package in pdf format! We promise it's nifty.</h5><br />
</div><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:40px;margin-bottom:40px;"><br />
If you have any questions or inquiries about the sponsorship package or potential benefits, don't hesitate to email our project manager Henry Barron at henry.barron@qgemteam.com. <br />
</div><br />
</div><br />
<br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/SponsorshipTeam:Queens Canada/Sponsorship2013-09-27T23:49:23Z<p>0sec8: </p>
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</div></div><br />
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<div class="offset1 span10 content"><br />
Queen's iGEM Team would like to thank all of our generous sponsors!<br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.queensu.ca/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Queen's University</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
We graciously thank the Department of Chemical Engineering, the Department of Biomedical and Molecular Sciences, the Department of Life Sciences and Biochemistry, the Department of Arts and Science, and the Department of Biology for their generous support of our team! In addition, we would like to thank the Faculty of Engineering and Applied Science for their continued support. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.pipetpeoplestore.com/index.cfm/fuseaction/home.home/index.htm" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Calibrate Inc.</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
Calibrate, Inc. provides pipette calibration, repair, and documentation services to scientific laboratories across North America. The largest and oldest independent pipette calibration and repair service in North America, Calibrate is a leader in quality in the industry and is known for delivering top-notch work and excellent customer service. <br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://qubitsystems.com/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Qubit Systems Inc.</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
Qubit Systems Inc. specializes in the design of instrumentation for research and teaching in the biological sciences, with a scope ranging from plant physiology to human respirometry. Their aim is to provide educational establishments, research institutes and companies with innovative, cost-effective equipment for research, teaching, and industrial applications. Qubit also manufactures and sells a broad range of gas analyzers, environmental sensors, instruments for aquatic biology, and the world's most sophisticated range of fluorescence imaging systems for chlorophyll, GFP and other biologically important compounds.<br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;margin-left:30px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.conocophillips.ca/EN/Pages/index.aspx" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">ConocoPhillips</span></a><br />
</di></div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="text-align:justify;"><br />
ConocoPhillips is a multinational energy corporation that explores for, produces, transports and markets crude oil, natural gas, natural gas liquids, liquefied natural gas and bitumen on a worldwide basis - energy that plays a foundational role in enabling global economic development and human progress. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Interested in </span><span class="rose">Sponsoring?</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;"><br />
Here at QGEM, we have a lot of passion for what we do. The tragedy though is that passion isn't exchangeable for reagents, which is something that we also need an abundance of. If you would like to show your support for our cause and sponsor us, download the sponsorship package and see what exactly it entails!<br />
</div> <br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span12" style="text-align:center;"><br />
<a href ="https://dl.dropboxusercontent.com/u/125588631/QGEM-FINAL.pdf"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/download.png" style="height:200px; float:center;"/><br />
</a><br />
</div><br />
<div class="row-fluid"><br />
<div class="span12" style="vertical-align:middle;text-align:center;"><br />
<h4>Money can't buy us love but it can buy us lab equipment.</h4><br />
<h5>Click here to download the sponsorship package in pdf format! We promise it's nifty.</h5><br />
</div><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:40px;margin-bottom:40px;"><br />
If you have any questions or inquiries about the sponsorship package or potential benefits, don't hesitate to email our project manager Henry Barron at henry.barron@qgemteam.com. <br />
</div><br />
</div><br />
<br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/SafetyTeam:Queens Canada/Safety2013-09-27T23:48:28Z<p>0sec8: </p>
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Keeping People </span><span class="rose">Safe</span></div><br />
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<div class="offset1 span10 content" style="text-align:justify;"><br />
Each team member on the Queen's iGEM 2013 team has received extensive lab safety and Workplace Hazardous Materials Information System (WHMIS) training as several hazardous compounds were encountered. These include ethidium bromide, a potential carcinogen and mutagen, and sodium azide, a potentially deadly chemical. Ethidium bromide was used to run gel electrophoresis while sodium azide was used in several mini chemotaxis assays to paralyze <i>Caenorhabditis elegans</i>. Proper protocols were followed in handling both chemicals, including the use of appropriate safety equipment and proper disposal techniques. As the research conducted this year is considered Level 1 work, all iGEM students worked in an officially approved Level 1 Biohazard Lab. All Canadian national biosafety standards and guidelines were followed. Furthermore, the project idea, methods and safety considerations were reviewed by two faculty advisors, Dr. Kenton Ko and Dr. Ian Chin-Sang, who are both members of the Biohazard Committee at Queen's University. <br />
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The bacterial strains used in the research are non-pathogenic <i>E. coli</i> strains, including K-12, XL1-Blue, and BL-21. These are rated as Biosafety Level 1 and do not pose a threat to laboratory workers, the general public or the environment. All measures outlined in the Material Safety Data Sheets (MSDS) and the biosafety regulations present at Queen's University were followed. These measures prevent our genetically modified E. coli strains from being introduced into the environment, and from posing any risk to security through malicious misuse by individuals, groups, or countries. <br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Application </span><span class="rose">Risks</span></div><br />
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<div class="offset1 span10 content" style="text-align:justify;"><br />
When changing foot odor into a compound that smells like bananas, it could have unpredictable effects such as luring insects or triggering allergic reactions. In order for this to become a commercial product, extensive testing with actual mosquitoes in regular outdoors environments as well as testing its effects on humans would be required. Since the product would require placing bacteria relatively close to human skin, such as the feet, precautions would need to be taken to ensure that the bacteria does not escape the product and colonize the shoes or feet instead. Further testing would be required to see if this would cause adverse effects.<br />
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<div class="offset1 span10 content" style="text-align:justify;"><br />
When working with the mosquito traps, they would have to be at optimal distance from human residences. If they are too far, then the impact would not be as great, but if they are too close, it may lure more mosquitoes to humans. With the idea and knowledge of making mosquito traps, people might get the idea of luring mosquitoes into traps and killing them. This could possibly cause the extinction of mosquitoes, which can potentially have adverse effects on the ecosystem.<br />
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The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br><br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our <a href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">Project</a> page for more details on yjgB and to see the data that we used to characterize this part.<br />
</div><br />
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<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
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</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content" style="text-align:justify;"><br />
The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our <a href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">Project</a> page for more details on yjgB and to see the data that we used to characterize this part.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
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</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our <a href="https://2013.igem.org/Team:Queens_Canada/Project">Project</a> page for more details on yjgB and to see the data that we used to characterize this part.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
</div><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T23:45:47Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our <a href="https://2013.igem.org/Team:Queens_Canada/Project">Project</a> page for more details on yjgB and to see the data we used to characterize this part.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T23:45:35Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our <a href="https://2013.igem.org/Team:Queens_Canada/Project">Project page</a> for more details on yjgB and to see the data we used to characterize this part.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
</div><br />
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</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T23:44:43Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<a href="https://igem.org/Main_Page"><br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The yjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
<br> Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, yjgB could play a useful role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable. See our project page for more details on yjgB and to see the data we used to characterize this part.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
</div><br />
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</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T23:43:04Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
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<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
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<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="text-align:justify;"><br />
<div class="offset1 span7 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO<sub>2</sub>, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in <i>E. coli</i> could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br><br><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
To that end we started off with using PCR to extract the endogenous genes from <i>E. coli</i>. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and a RBS did not prove successful despite numerous tries. This ended our work with this gene. <br />
</div><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/f4/TnaA_gel.jpg" style="height:250px;"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content" style="text-align:justify;">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, <i>Asaia</i> sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T23:42:07Z<p>0sec8: </p>
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<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="text-align:justify;"><br />
<div class="offset1 span7 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO<sub>2</sub>, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in <i>E. coli</i> could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br><br><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
To that end we started off with using PCR to extract the endogenous genes from <i>E. coli</i>. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and a RBS did not prove successful despite numerous tries. This ended our work with this gene. <br />
</div><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/f4/TnaA_gel.jpg" style="height:250px;"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content" style="text-align:justify;">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, Asaia sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T23:39:55Z<p>0sec8: </p>
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</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="text-align:justify;"><br />
<div class="offset1 span7 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO<sub>2</sub>, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in E. coli could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br><br><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
To that end we started off with using PCR to extract the endogenous genes from E. coli. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and an RBS did not prove successful despite numerous tries. This ended our work with this gene. <br />
</div><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/f4/TnaA_gel.jpg" style="height:250px;"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content" style="text-align:justify;">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, Asaia sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T23:39:25Z<p>0sec8: </p>
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</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="text-align:justify;"><br />
<div class="offset1 span7 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO<sub>2</sub>, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in E. coli could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br><br><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
To that end we started off with using PCR to extract the endogenous genes from E. coli. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and an RBS did not prove successful despite numerous tries. This ended our work with this gene. <br />
</div><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/f4/TnaA_gel.jpg" style="height:250px;"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span style="line-height:50px"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content" style="text-align:justify;">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, Asaia sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T23:37:47Z<p>0sec8: </p>
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<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="text-align:justify;"><br />
<div class="offset1 span7 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO<sub>2</sub>, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in E. coli could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br><br><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
To that end we started off with using PCR to extract the endogenous genes from E. coli. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and an RBS did not prove successful despite numerous tries. This ended our work with this gene. <br />
</div><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/f4/TnaA_gel.jpg" style="height:250px;"/><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content" style="text-align:justify;">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, Asaia sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T23:31:13Z<p>0sec8: </p>
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<div class="header-bg"><br />
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10 content" style="text-align:justify;"><br />
Our idea of a mosquito repellent aims to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that mosquitoes infected with the malarial parasite, <i>Plasmodium falciparum</i>, are four times more attracted to foot odour than non-infected mosquitoes. By removing the volatile compounds that cause these smells, we hypothesize that this will effectively decrease the ability of mosquitoes to find humans. Our project thus focuses on creating a genetic and enzymatic pathway to remove those volatile compounds. It begins with the uptake of isovaleric acid, a known mosquito semiochemical that is present is foot odour, and converts it into banana smell. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria infection rates. <br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span8 content" style="text-align:justify;"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the <i>E. coli genome</i>, we designed PCR primers so that the AtoE gene could be replicated with the correct biobrick prefix and suffix. The size of the AtoE gene is 1323 bp, but with the addition of the standard biobrick cut sites (43bp), a 1.4kb band was expected and was seen on the gel of the PCR products (<i>on right</i>). <br />
</div><br />
<div class="span3"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span8 content" style="text-align:justify;"><br />
Carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (NPT) are endogenously expressed in <i>Nocardia iowensis</i>. NCAR is a highly non-specific carboxylic acid reductase that converts fatty acids such as isovaleric acid into aldehydes. NPT is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. NCAR was <a href="http://parts.igem.org/Part:BBa_K902062:Design">submitted</a> as a single part by Calgary iGEM 2012, but it is dependent on NPT for full function. We thus sought to link these two enzymes together to improve Calgary's part. <br />
<br><br />
</div><br />
<div class="span3"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
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</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
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<div class="row-fluid"><br />
<div class="offset1 span6 content" style="text-align:justify;"><br />
YjgB is a non-specific zinc-containing alcohol dehydrogenase endogenously expressed in <i>E. coli</i>. It reduces a number of different aldehydes to their corresponding alcohol using NADPH as a co-substrate. In this instance, we planned to use yjgB to reduce isoamyl aldehyde to isoamyl alcohol. We successful in amplifying yjgB by PCR, cloning it into pSB1C3 with a high constitutive expression cassette, and we saw very high protein at the predicted size of 36kDa on an SDS PAGE gel. We verified our biobrick before sending it into the registry and found the sequence to be an exact match for the putative sequence found on GenBank. Over the next week we will be using mass spectrometry and an NADPH activity assay to verify the amino acid sequence of the part and quantify its specific activity towards isobutyraldehyde. This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
</div><br />
<div class="span4"><img style = "height:300px;" src="https://static.igem.org/mediawiki/2013/0/06/Screen_Shot_2013-09-27_at_5.51.53_PM.png" style="height:500px;margin-top:30px;"></div></div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;text-align:justify;"><br />
Alcohol acetyl-transferase 1 (ATF1) (<a href="http://parts.igem.org/Part:BBa_J45014">BBa_J45014</a>) is an enzyme endogenous to <i>Saccharomyces cerevisiae</i> and was first used by MIT iGEM 2006. It catalyzes the conversion of isoamyl alcohol to isoamyl acetate, which has a banana smell. This will complete the neutralization of isovaleric acid. We received the ATF1 gene from the Biobrick registry where we transformed and cut the part to be placed in with other genes in our pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;text-align:justify;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;text-align:justify;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is naturally used to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals as either attractants or repellants with a GPCR system.<br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/OverviewTeam:Queens Canada/Project/Overview2013-09-27T23:23:49Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="tidal"><br />
<div class="row-fluid"><br />
<div class="offset1 span11" style="margin-top:40px; margin-bottom:40px;"><br />
<span style="line-height:50px"><span class="black">Biosynthesis and Breakdown of </span><span class="rose">Human Odour Compounds </span><span class="black">for the Behavioural Manipulation of </span><span class="rose">Malarial Mosquitoes</span></span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<div class="content" style="margin-bottom:40px;text-align:justify;"><br />
Mosquitoes can serve as vectors for a variety of diseases in endemic areas, including malaria, West Nile and dengue fever. The World Health Organization reported that there were 219 million cases of malaria in 2010 alone, 660,000 of which resulted in death (citation). Mosquitoes have been found to use several airborne chemical cues to locate a blood-meal, which act as ligands for its olfactory receptors. These chemical cues include a host’s skin emanations, exhaled air, and urine. In particular, the African mosquito <i>Anopheles gambiae</i> was shown to be four times more attracted to foot odour when infected with the malarial parasite, <i>Plasmodium falciparum</i> (Zwiebel and Takken, 2011). In our project, we chose to focus on isovaleric acid, which is a key component of foot odour and is produced by <i>Staphylococcus epidermidis</i>, a resident species of normal cutaneous flora.<br />
<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/OverviewTeam:Queens Canada/Project/Overview2013-09-27T23:17:04Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
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<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
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<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
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</div><br />
<br />
<div class="tidal"><br />
<div class="row-fluid"><br />
<div class="offset1 span11" style="margin-top:40px; margin-bottom:40px;"><br />
<span style="line-height:50px"><span class="black">Biosynthesis and Breakdown of </span><span class="rose">Human Odour Compounds </span><span class="black">for the Behavioural Manipulation of </span><span class="rose">Malarial Mosquitoes</span></span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<div class="content" style="margin-bottom:40px;text-align:justify;"><br />
Mosquitoes can serve as vectors for a variety of diseases in endemic areas, including malaria, West Nile and dengue fever. The World Health Organization reported that there were 219 million cases of malaria in 2010 alone, 660,000 of which resulted in death. Mosquitoes have been found to use several airborne chemical cues to locate a blood-meal, which act as ligands for its olfactory receptors. These chemical cues include a host’s skin emanations, exhaled air, and urine. In particular, the African mosquito <i>Anopheles gambiae</i> was shown to be four times more attracted to foot odour when infected with the malarial parasite, <i>Plasmodium falciparum</i> (Zwiebel and Takken, 2011). In our project, we chose to focus on isovaleric acid, which is a key component of foot odour and is produced by <i>Staphylococcus epidermidis</i>, a resident species of normal cutaneous flora (citation).<br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:40px;margin-bottom:40px;"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/e/e6/Qgem_info.png" style="overflow:hidden;" /><br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/OverviewTeam:Queens Canada/Project/Overview2013-09-27T23:05:23Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
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<br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<br />
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<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
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<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
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</div><br />
<br />
<div class="tidal"><br />
<div class="row-fluid"><br />
<div class="offset1 span11" style="margin-top:40px; margin-bottom:40px;"><br />
<span class="black">Biosynthesis and Breakdown of </span><span class="rose">Human Odour Compounds </span><span class="black">for the Behavioural Manipulation of </span><span class="rose">Malarial Mosquitoes</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<div class="content" style="margin-bottom:40px;"><br />
Mosquitoes can serve as vectors for a variety of diseases in endemic areas, including malaria, West Nile and dengue fever. The World Health Organization reported that there were 219 million cases of malaria in 2010 alone, 660,000 of which resulted in death. Mosquitoes have been found to use several airborne chemical cues to locate a blood-meal, which act as ligands for its olfactory receptors. These chemical cues include a host’s skin emanations, exhaled air, and urine. In particular, the African mosquito <i>Anopheles gambiae</i> was shown to be four times more attracted to foot odour when infected with the malarial parasite, <i>Plasmodium falciparum</i> (Zwiebel and Takken, 2011). In our project, we chose to focus on isovaleric acid, which is a key component of foot odour and is produced by <i>Staphylococcus epidermidis</i>, a resident species of normal cutaneous flora (citation).<br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:40px;margin-bottom:40px;"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/e/e6/Qgem_info.png" style="overflow:hidden;" /><br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:42:23Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea of a mosquito repellent aims to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that mosquitoes infected with the malarial parasite, <i>Plasmodium falciparum</i>, are four times more attracted to foot odour than non-infected mosquitoes. By removing the volatile compounds that cause these smells, we hypothesize that this will effectively decrease the ability of mosquitoes to find humans. Our project thus focuses on creating a genetic and enzymatic pathway to remove those volatile compounds. It begins with the uptake of isovaleric acid, a known mosquito semiochemical that is present is foot odour, and converts it into banana smell. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria infection rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the <i>E. coli genome</i>, we designed PCR primers so that the AtoE gene could be replicated with the correct biobrick prefix and suffix. The size of the AtoE gene is 1323 bp, but with the addition of the standard biobrick cut sites (43bp), a 1.4kb band was expected and was seen on the gel of the PCR products (<i>on right</i>). <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (NPT) are endogenously expressed in <i>Nocardia iowensis</i>. NCAR is a highly non-specific carboxylic acid reductase that converts fatty acids such as isovaleric acid into aldehydes. NPT is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. NCAR was <a href="http://parts.igem.org/Part:BBa_K902062:Design">submitted</a> as a single part by Calgary iGEM 2012, but it is dependent on NPT for full function. We thus sought to link these two enzymes together to improve Calgary's part. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is a non-specific zinc-containing alcohol dehydrogenase endogenously expressed in <i>E. coli</i>. It reduces isoamyl aldehyde into isoamyl alcohol. This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (<a href="http://parts.igem.org/Part:BBa_J45014">BBa_J45014</a>) is an enzyme endogenous to <i>Saccharomyces cerevisiae</i> and was first used by MIT iGEM 2006. It catalyzes the conversion of isoamyl alcohol to isoamyl acetate, which has a banana smell. This will complete the neutralization of isovaleric acid. We received the ATF1 gene from the Biobrick registry where we transformed and cut the part to be placed in with other genes in our pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is naturally used to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals as either attractants or repellants with a GPCR system.<br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:32:46Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
</div><br />
</div><br />
<br />
<div class="menuc" style="font-size:1.3em;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea of a mosquito repellent aims to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that mosquitoes infected with the malarial parasite, <i>Plasmodium falciparum</i>, are four times more attracted to foot odour than non-infected mosquitoes. By removing the volatile compounds that cause these smells, we hypothesize that this will effectively decrease the ability of mosquitoes to find humans. Our project thus focuses on creating a genetic and enzymatic pathway to remove those volatile compounds. It begins with the uptake of isovaleric acid, a known mosquito semiochemical that is present is foot odour, and converts it into banana smell. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria infection rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (NPT) are endogenously expressed in <i>Nocardia iowensis</i>. NCAR is a highly non-specific carboxylic acid reductase that converts fatty acids such as isovaleric acid into aldehydes. NPT is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. NCAR was <a href="http://parts.igem.org/Part:BBa_K902062:Design">submitted</a> as a single part by Calgary iGEM 2012, but it is dependent on NPT for full function. We thus sought to link these two enzymes together to improve Calgary's part. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is a non-specific zinc-containing alcohol dehydrogenase endogenously expressed in <i>E. coli</i>. It reduces isoamyl aldehyde into isoamyl alcohol. This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (<a href="http://parts.igem.org/Part:BBa_J45014">BBa_J45014</a>) is an enzyme endogenous to <i>Saccharomyces cerevisiae</i> and was first used by MIT iGEM 2006. It catalyzes the conversion of isoamyl alcohol to isoamyl acetate, which has a banana smell. This will complete the neutralization of isovaleric acid. We received the ATF1 gene from the Biobrick registry where we transformed and cut the part to be placed in with other genes in our pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:23:01Z<p>0sec8: </p>
<hr />
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QGEM 2013 PRESENTS:<br />
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<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (NPT) are endogenously expressed in <i>Nocardia iowensis</i>. NCAR is a highly non-specific carboxylic acid reductase that converts fatty acids such as isovaleric acid into aldehydes. NPT is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. NCAR was <a href="http://parts.igem.org/Part:BBa_K902062:Design">submitted</a> as a single part by Calgary iGEM 2012, but it is dependent on NPT for full function. We thus sought to link these two enzymes together to improve Calgary's part. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is a non-specific zinc-containing alcohol dehydrogenase endogenously expressed in <i>E. coli</i>. It reduces isoamyl aldehyde into isoamyl alcohol. This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (<a href="http://parts.igem.org/Part:BBa_J45014">BBa_J45014</a>) is an enzyme endogenous to <i>Saccharomyces cerevisiae</i> and was first used by MIT iGEM 2006. It catalyzes the conversion of isoamyl alcohol to isoamyl acetate, which has a banana smell. This will complete the neutralization of isovaleric acid. We received the ATF1 gene from the Biobrick registry where we transformed and cut the part to be placed in with other genes in our pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br><br />
<br><br />
<br><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:22:26Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
</div><br />
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<br />
<div class="menuc" style="font-size:1.3em;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) and phosphopantetheinyl transferase (NPT) are endogenously expressed in <i>Nocardia iowensis</i>. NCAR is a highly non-specific carboxylic acid reductase that converts fatty acids such as isovaleric acid into aldehydes. NPT is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. NCAR was <a href="http://parts.igem.org/Part:BBa_K902062:Design">submitted</a> as a single part by Calgary iGEM 2012, but it is dependent on NPT for full function. We thus sought to link these two enzymes together to improve Calgary's part. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is a non-specific zinc-containing alcohol dehydrogenase endogenously expressed in <i>E. coli</i>. It reduces isoamyl aldehyde into isoamyl alcohol. This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (<a href="http://parts.igem.org/Part:BBa_J45014">BBa_J45014</a>) is an enzyme endogenous to <i>Saccharomyces cerevisiae</i> and was first used by MIT iGEM 2006. It catalyzes the conversion of isoamyl alcohol to isoamyl acetate, which has a banana smell. This will complete the neutralization of isovaleric acid. We received the ATF1 gene from the Biobrick registry where we transformed and cut the part to be placed in with other genes in our pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:11:00Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
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<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
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<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
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<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is non-specific zinc-containing alcohol dehydrogenase endogenously expressed in E. coli. It will reduce isoamyl aldehyde into isoamyl alcohol.This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:60px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (BBa_J45014) is a enzyme endogenous to Saccharomyces cerevisiae which converts isoamyl alcohol to isoamyl acetate. This will complete the neutralization of isovaleric acid. This enzyme was first used by MIT iGEM 2006. We received the ATF gene from the biobrick registry where we transformed and cut the part to be placed in with the other genes in the pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:40px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. The graph to the right shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between <i>C. elegans</i> motility response to the control and test samples. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:08:07Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
</div><br />
</div><br />
<br />
<div class="menuc" style="font-size:1.3em;"><br />
<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
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<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
</div><br />
<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
</div><br />
<div class="span4"><br />
<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content"><br />
Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is non-specific zinc-containing alcohol dehydrogenase endogenously expressed in E. coli. It will reduce isoamyl aldehyde into isoamyl alcohol.This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:40px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (BBa_J45014) is a enzyme endogenous to Saccharomyces cerevisiae which converts isoamyl alcohol to isoamyl acetate. This will complete the neutralization of isovaleric acid. This enzyme was first used by MIT iGEM 2006. We received the ATF gene from the biobrick registry where we transformed and cut the part to be placed in with the other genes in the pathway. <br />
<br><br />
</div><br />
<div class="span4"><br />
<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span7 content" style="margin-bottom:40px;"><br />
<i>Caenorhabditis elegans</i> has impressive chemotaxis behaviour which is used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined<a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. <br />
</div><br />
<div class="span4"><br />
<img style="height:500px;text-align:center;" src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"/><br />
</div><br />
</div><br />
<br />
<br><br />
The above shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between C. elegans motility response to the control and test samples. <br />
<br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T22:03:23Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
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<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
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Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
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<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
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<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
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AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
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In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
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<img style="height:250px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"/><br />
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<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
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Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. <br />
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<img style="height:300px;text-align:center;" src="https://static.igem.org/mediawiki/2013/8/8f/Ncar_npt.png"/><br />
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<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
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YjgB is non-specific zinc-containing alcohol dehydrogenase endogenously expressed in E. coli. It will reduce isoamyl aldehyde into isoamyl alcohol.This is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
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<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
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Alcohol acetyl-transferase 1 (ATF1) (BBa_J45014) is a enzyme endogenous to Saccharomyces cerevisiae which converts isoamyl alcohol to isoamyl acetate. This will complete the neutralization of isovaleric acid. This enzyme was first used by MIT iGEM 2006. We received the ATF gene from the biobrick registry where we transformed and cut the part to be placed in with the other genes in the pathway. <br />
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<center><img src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"></center><br />
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<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
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Caenorhabditis elegans has impressive chemotaxis behaviour, used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
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In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined <a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. <br />
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<center><img src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"> </center><br />
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The above shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between C. elegans motility response to the control and test samples. <br />
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<div id="sponsorship-text" class="black" style="font-size:1.1em;">A huge thanks to our sponsors:</div><br />
<div id="sponsorship-small"><br />
<img style="height:130px;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg" /><br />
<img style="height:150px;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg" /><br />
<img style="height:150px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg" /><br />
<img style="height:80px;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg" /><br />
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<span class="black">This Year's </span><span class="rose">Part</span><br />
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<div class="offset1 span10 content"><br />
The YjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we used YjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
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<center><img src="https://static.igem.org/mediawiki/2013/0/06/Screen_Shot_2013-09-27_at_5.51.53_PM.png" style="height:500px;margin-top:30px;"></center><br />
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Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, YjgB is thought to play a very important role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable.<br />
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<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
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<div></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/AttractTeam:Queens Canada/Project/Attract2013-09-27T05:17:25Z<p>0sec8: </p>
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Trapping </span><span class="black">Mosquitoes</span></div><br />
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<div class="offset1 span6 content">We intend to devise a mosquito trap where bacteria will synthesize the chemicals found in foot and body odour. Once the mosquitoes are trapped, we aim to further resolve disease by infecting the mosquitoes to sterilize, control, or destroy the vectors. Inspired by the recent finding of Dr. Fredros Okumu that a synthetic odour blend could be more attractive to a mosquito than a living human, we set out to devise a mosquito trap using synthetic biology. For example, a strain of yeast or bacteria could be engineered to produce CO2, body odour, and foot odour to lure the insects to their death with relative specificity. </div><br />
<div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/4a/Mosquito_graph.jpg" style="height:250px;" /></div><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">A Solution to </span><span class="rose">Insecticide and Drug Resistance</span></div></div><br />
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<div class="offset1 span10 content">A major roadblock in the eradication of malarial mosquitoes is their tendency to adapt their behavior and develop resistance to the solutions we devise. For example, insecticide-treated mosquito nets are becoming less and less effective because mosquitoes can develop diurnal feeding patterns to avoid them (<a href="http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract">http://jid.oxfordjournals.org/content/early/2012/09/20/infdis.jis565.abstract</a>). Similarly, the malaria parasite, <i>Plasmodium falciparum</i> can quickly become resistant to new drugs we develop to destroy it. Resistant strains have emerged even for the newest and most potent anti-malarial drug we have at our disposal, artemisinin (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495232/</a>). Our solution circumvents this problem. The same thing that would grant mosquitoes resistance to the traps – i.e. becoming less sensitive to body odour – would also make it more difficult for them to find their human hosts. In this way, it may be possible to slow or even completely prevent mosquitoes from developing resistance to our intervention.</div></div><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">Indole Production</span><span class="black"> as a Proof of Concept</span></div></div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10 content">We set out to synthesize indole as our first attractant. We chose the molecule for two reasons. First of all, it is produced endogenously in <i>E. coli</i> so the genes responsible for its synthesis and export, TnaA and TnaB, provided a natural target for PCR amplification. Once amplified, we could then clone the sequences into a plasmid with a strong constitutive promoter to produce the attractant in high yield. The second reason we chose indole is that there are known oderant binding proteins specific for it, and these receptors are highly conserved across mosquito species (<a href=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020388/</a>).<br />
<br />
<br><br><br />
<br />
In our pathway, TnaB acts as a transporter, allowing for uptake of tryptophan into the bacteria. TnaA then acts as an enzyme to degrade tryptophan to indole. This ultimately allows for the production of indole in cells and if implemented in E. coli could be used to generate large amounts of the compound to act as an attractant. <br />
</div><br />
</div><br />
<br><br />
<center style="margin: 40 0;"><img src="https://static.igem.org/mediawiki/2013/3/3b/Tnaa_pathway.png" /></center><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span 6"><br />
To that end we started off with using PCR to extract the endogenous genes from E. coli. We were successful in this endeavour, as the gel to the right shows 1.4kb bands which is the appropriate size for TnaA. However unfortunately, our attempts to ligate TnaA, TnaB, and an RBS did not prove successful. <br />
</div><br />
<div class="span4"><br />
<img src="https://2013.igem.org/File:1044794_10151741231569580_216139266_n.jpg" style="height:400px;"/><br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Future Directions: </span><span class="rose">Infecting Mosquitoes</span></div></div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content">If we were to use a species of bacteria in our trap that infected the mosquitoes and specifically affected their biology, such as by inhibiting the synthesis or action of transglutaminase in male mosquitoes, we could potentially do even more to prevent malaria. Inhibition of transglutaminase has been shown to prevent the formation of the male mating plug that is used to keep seminal fluid sealed in place. Because female mosquitoes only mate once in their life, mating with a male infected with the transglutaminase-blocking bacteria would sterilize the female as well, effectively doubling the number of mosquitoes the trap affects.</div></div><br />
<br />
<div class="row-fluid" style="margin-top:20px;margin-bottom:30px;"><br />
<div class="offset1 span10 content">Infecting mosquitoes with bacteria is not a new concept, and there are a number of options in terms of the chassis that could be used. For example, Lausanne iGEM in 2010 characterized a new chassis, Asaia sp. that can live in the intestinal tract of mosquitoes and be transmitted both horizontally and vertically between mosquitoes. <i>Wolbachia</i> sp. is another strain of bacteria that has been shown to infect the mosquito that carries Dengue fever, <i>Aedes aegipti </i>with good stability (<a href="http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html">http://www.nature.com/nature/journal/v476/n7361/full/nature10356.html</a>). If this strain could be shown to similarly affect <i>Anopheles gambiae</i>, it might also make an excellent choice of chassis for this purpose.</div></div><br />
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</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/RepelTeam:Queens Canada/Project/Repel2013-09-27T05:17:06Z<p>0sec8: </p>
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<div class="header-bg"><br />
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QGEM 2013 PRESENTS:<br />
</div><br />
<div class="header-logo"><br />
<img src="https://static.igem.org/mediawiki/2013/c/c7/Projct.png" style="height:80px;" /><br />
<a href="https://igem.org/Main_Page"><br />
<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
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<br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
<li id="project"><a href="https://2013.igem.org/Team:Queens_Canada/Project">PROJECT</a></li><br />
<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
</ul><br />
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<br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Mosquito </span><span class="rose">Repellent</span></div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
<img src="https://static.igem.org/mediawiki/2013/0/0d/Iso_pathway_2.jpg" style="width:90%;text-align:center;" /><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">AtoE</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
<br />
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.<br />
<br><br><br />
In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The size of the AtoE gene is 1323bp. With the addition of 43bp from the standard biobrick prefix and suffix, we should get a band around 1.4kb as shown by the gel below of the PCR products we got. <br />
<center><img src ="https://static.igem.org/mediawiki/2013/5/5c/AtoE_PCR_product.png"></center><br />
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</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">NCAR and NPT</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">YjgB</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content"><br />
YjgB is non-specific zinc-containing alcohol dehydrogenase endogenously expressed in E. coli. It will reduce isoamyl aldehyde into isoamyl alcohol.<br />
<br><br><br />
YjgB is the part that is being submitted this year. More details on this can be found on the <a href="https://2013.igem.org/Team:Queens_Canada/Parts">Parts</a> page on the wiki. <br />
<br />
<br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">ATF1</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-bottom:40px;"><br />
Alcohol acetyl-transferase 1 (ATF1) (BBa_J45014) is a enzyme endogenous to Saccharomyces cerevisiae which converts isoamyl alcohol to isoamyl acetate. This will complete the neutralization of isovaleric acid. This enzyme was first used by MIT iGEM 2006. We received the ATF gene from the biobrick registry where we transformed and cut the part to be placed in with the other genes in the pathway. <br />
<br><br />
<div class="span4"><br />
<img src="https://static.igem.org/mediawiki/2013/f/fa/ATF_gel.png"><br />
</div><br />
<br />
<br />
<br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:20px;margin-bottom:10px;"><span class="rose" style="font-size:1.5em">Testing Isovaleric Acid</span></div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-bottom:40px;"><br />
Caenorhabditis elegans has impressive chemotaxis behaviour, used naturally to seek out food sources. It has been shown to respond to a wide range of both water-soluble and volatile chemicals with a GPCR system, as either attractants or repellants. <br />
<br><br />
In order to test the effectiveness of our part, it was necessary to create an assay to test our completed part against. Using the assay methodology pioneered by QGEM 2011 (Margie et. al. 2013), worms were placed in a common starting area and their movement in response to a control substance and to the test odorant was observed. With an anaesthetic at target sites to immobilize worms at the end of each trial, the number of worms in the test and control quadrants were counted to calculate a chemotactic index. More details on how this assay works can be outlined <a href="http://www.jove.com/video/50069/c-elegans-chemotaxis-assay"> here</a>.<br />
<br><br />
Isovaleric acid solutions at 1M, 7:1, and 99% concentrations were used. A total of 38 chemotaxis assay plates were run. <br />
<br />
<center><img src = "https://static.igem.org/mediawiki/igem.org/c/c5/Chemotaxisboxplots.jpg"> </center><br />
<br><br />
The above shows our results from the standard graph for our chemotaxis assay. According to statistical analysis, there was no significant difference between C. elegans motility response to the control and test samples. <br />
<br />
<br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset2 span8" id="sponsorship-large"><br />
<div id="sponsorship-text" class="black" style="font-size:1.1em;">A huge thanks to our sponsors:</div><br />
<div id="sponsorship-small"><br />
<img style="height:130px;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg" /><br />
<img style="height:150px;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg" /><br />
<img style="height:150px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg" /><br />
<img style="height:80px;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg" /><br />
</div><br />
</div><br />
</div><br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/Project/OverviewTeam:Queens Canada/Project/Overview2013-09-27T05:16:43Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<ul><br />
<li id="home"><a href="https://2013.igem.org/Team:Queens_Canada">HOME</a></li><br />
<li id="team"><a href="https://2013.igem.org/Team:Queens_Canada/Team">TEAM</a></li><br />
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<li id="outreach"><a href="https://2013.igem.org/Team:Queens_Canada/Outreach">OUTREACH</a></li><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSORS</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<br />
<div class="menuc disa" style="display:none;"><br />
<ul><br />
<li id="parts"><a href="https://2013.igem.org/Team:Queens_Canada/Parts">PARTS</a></li><br />
<li id="safety"><a href="https://2013.igem.org/Team:Queens_Canada/Safety">SAFETY</a></li><br />
<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<br />
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<div class="span4 project-buttons repel"><br />
<a class="rep" href="https://2013.igem.org/Team:Queens_Canada/Project/Repel">REPEL</a><br />
</div><br />
<div class="span4 project-buttons overview"><br />
<a class="test" href="https://2013.igem.org/Team:Queens_Canada/Project/Overview">OVERVIEW</a><br />
</div><br />
<div class="span4 project-buttons attract"><br />
<a class="att" href="https://2013.igem.org/Team:Queens_Canada/Project/Attract">ATTRACT</a><br />
</div><br />
</div><br />
<br />
<div class="tidal"><br />
<div class="row-fluid"><br />
<div class="offset1 span11" style="margin-top:40px; margin-bottom:40px;"><br />
<span class="black">Using </span><span class="rose">Foot Odour </span><span class="black">in the Fight Against </span><span class="rose">Malaria</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10"><br />
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The drone of the mosquito is a common sound. On camping trips it serves as a herald of itchy swells and signals that it is time to break out the bug spray. In many other parts of the world however, this sound means something completely different. It signals the presence of a vector for diseases such as malaria, West Nile and dengue fever. Mosquitoes are responsible for the widespread of these sicknesses; there were 219 million cases of malaria in 2010 alone, 660,000 of which resulted in death. Mosquitoes have been found to use several air-bourne chemical cues to locate a blood-meal, which act as ligands for its odorant receptors. These include a host’s skin emanations, exhaled air, and urine. The host’s foot odour in particular (Zwiebel and Takken, 2011), one key component of which is isovaleric acid produced by <i>Staphylococcus epidermidis</i>, a resident species of normal cutaneous flora (citation).<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T05:14:17Z<p>0sec8: </p>
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<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
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<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The YjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we used YjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
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<center><img src="https://static.igem.org/mediawiki/2013/f/f6/YjgB.jpg" style="height:500px;margin-top:30px;"></center><br />
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Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, YjgB is thought to play a very important role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable.<br />
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To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
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<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The YjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we used YjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
</div><br />
</div><br />
<br />
<center><img src="https://static.igem.org/mediawiki/2013/f/f6/YjgB.jpg" style="height:500px;margin-top:30px;"></center><br />
<br />
<div class="row-fluid" style="margin-top:30px;"><br />
<div class="offset1 span10 content"><br />
Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, YjgB is thought to play a very important role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<div class="row-fluid"><br />
<div class="offset1 span10" style="margin-top:40px;"><span class="black">TnaA and TnaB BioBrick Part</span></div><br />
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<div class="offset1 span10 content"><br />
TnaA and TnaB are used for converting exogenous tryptophan into indole. TnaB is one of three known transporters for tryptophan in <i>E. coli</i> and TnaA (trytophanase) degrades tryptophan to indole. Select "Lab Book" to view our procedure in detail!<br />
</div></div><br />
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<iframe width="800" height="600" style = "margin-left: 10%;margin-bottom:5%;" scrolling="yes" src="https://synbiota.ca/projects/85/show_as_embedded"></iframe><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/ATF1Team:Queens Canada/ATF12013-09-27T04:58:34Z<p>0sec8: </p>
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
</ul><br />
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<div class="offset1 span10" style="margin-top:40px;"><span class="black">ATF1 BioBrick Part</span></div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px;"><br />
<div class="offset1 span10 content"><br />
Acetyl transferase 1 (ATF1) was a BioBrick created by MIT in 2006 as part of their iGEM project. It is derived from <i>Saccharomyces cerevisiae</i> and it catalyzes the conversion of isoamyl alcohol to isoamyl acetate, a compound that gives off a banana scent. This is the final step of our pathway, completing the neutralization of isovaleric acid. Select "Lab Book" to view our procedure in detail!<br />
</div></div><br />
<br />
<iframe width="800" height="600" style="margin-left:10%;margin-bottom:5%;" scrolling="yes" src="https://synbiota.ca/projects/87/show_as_embedded"></iframe><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/YjgBTeam:Queens Canada/YjgB2013-09-27T04:58:13Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<div class="offset1 span10" style="margin-top:40px;"><span class="black">YjgB BioBrick Part</span></div><br />
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<div class="row-fluid" style="margin-top:30px;margin-bottom:30px;"><br />
<div class="offset1 span10 content"><br />
This medium chain zinc-containing enzyme of the dehydrogenase/reductase family is NADPH-dependent. It has been shown to reduce acetaldehyde and isobutyraldehyde to their corresponding alcohols. Select "Lab Book" to view our procedure in detail!<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/NCAR_NPTTeam:Queens Canada/NCAR NPT2013-09-27T04:57:55Z<p>0sec8: </p>
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<div class="offset1 span10" style="margin-top:40px;"><span class="black">NCAR-NPT BioBrick Part</span></div><br />
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Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate. Select 'Lab Book' to view our project in detail!<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/AtoETeam:Queens Canada/AtoE2013-09-27T04:57:30Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="sponsorship"><a href="https://2013.igem.org/Team:Queens_Canada/Sponsorship">SPONSOR</a></li><br />
<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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AtoE is a membrane transporter of short chain fatty acids. This gene is normally expressed in E. coli but in our project, the plan is to constitutively express this gene such that it is constantly uptaking surrounding isovaleric acid. Once within the bacteria, other reactions ensue. Select "Lab Book" to view our procedure in detail!<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/NotebookTeam:Queens Canada/Notebook2013-09-27T04:57:15Z<p>0sec8: </p>
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<div class="offset1 span10" style="margin-top:40px;"><span class="black">Lab </span><span class="rose">Journal</span></div><br />
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<div class="offset1 span10 content"><br />
Want to know what we were <i>really </i> up to this summer? Check out the following links to read about our successes, our failures, and the downright bizarre problems that we encountered. <br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-bottom:10px;"><br />
<div class="offset1 span10 content"><br />
<a href="https://2013.igem.org/Team:Queens_Canada/AtoE" style="font-size:0.4em;"><span class="rose">(1) Short chain fatty acid membrane transporter (AtoE)</span></a><br />
</div></div><br />
<br />
<div class="row-fluid" style="margin-bottom:10px;"><br />
<div class="offset1 span10 content"><br />
<a href="https://2013.igem.org/Team:Queens_Canada/NCAR_NPT" style="font-size:0.4em;"><span class="rose">(2) Carboxylic acid reductase (NCAR) and phosphopantetheinyl transferase (NPT)</span></a><br />
</div></div><br />
<br />
<div class="row-fluid" style="margin-bottom:10px;"><br />
<div class="offset1 span10 content"><br />
<a href="https://2013.igem.org/Team:Queens_Canada/YjgB" style="font-size:0.4em;"><span class="rose">(3) Aldehyde reductase (yjgB)</span></a><br />
</div></div><br />
<br />
<div class="row-fluid" style="margin-bottom:10px;"><br />
<div class="offset1 span10 content"><br />
<a href="https://2013.igem.org/Team:Queens_Canada/ATF1" style="font-size:0.4em;"><span class="rose">(4) Acetyl transferase (ATF1)</span></a><br />
</div></div><br />
<br />
<div class="row-fluid" style="margin-bottom:30px;"><br />
<div class="offset1 span10 content"><br />
<a href="https://2013.igem.org/Team:Queens_Canada/TnaA_TnaB" style="font-size:0.4em;"><span class="rose">(5) Tryptophan to Indole (TnaA and TnaB)</span></a><br />
</div></div><br />
<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/JudgingTeam:Queens Canada/Judging2013-09-27T04:56:54Z<p>0sec8: </p>
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<span class="black">Judging</span><span class="rose"> Criteria</span><br />
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<ul><br />
<br />
<p><li>We completed the registration for iGEM 2013.</li></p><br />
<p><li>We completed the iGEM 2013 judging form.</li></p><br />
<p><li>We completed the QGEM 2013 team wiki.</li></p><br />
<p><li>We presented a poster and a talk for our project at the North America 2013 iGEM Jamboree.</li></p><br />
<p><li>We documented and submitted a new part, yjgB, to the Biobrick Registry.</li></p><br />
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</ul><br />
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<ul><br />
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<p><li>We experimentally validated that our new yjgB part works as expected.</li></p><br />
<p><li>We documented the characterization of our part yjgB on the “Main Page” of its Entry in the Biobrick Registry.</p></li><br />
<p><li>We documented and submitted a new part, yjgB, to the Biobrick Registry.</li></p><br />
<p><li>We worked to increase awareness to the issues affecting the security, safety and ethical issues affecting synthetic biology through several means. We developed internet video presentations, conducted a radio interview with CBC Radio One, were featured in a news article in a local newspaper and organized debates for upper-year high school students regarding the ethics of synthetic biology through participation in the Shad Valley program.</li></p><br />
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<div class="offset1 span10" style="margin-top:20px;font-size:0.5em;margin-bottom:1px;border:1px red;"><span class="rose">Gold</span></div><br />
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<div class="offset1 span10 content border" style="margin-bottom:40px;"><br />
<br />
<ul><br />
<br />
<p><li>We helped iGEM Toronto by providing a team of volunteers to aid with the setup of the North American iGEM Jamboree.</li></p><br />
<p><li>We worked to increase awareness to the issues affecting the security, safety and ethical issues affecting synthetic biology through several means. We developed internet video presentations, conducted a radio interview with CBC Radio One, were featured in a news article in a local newspaper and organized debates for upper-year high school students regarding the ethics of synthetic biology through participation in the Shad Valley program.</li></p><br />
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</ul><br />
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<img src="https://static.igem.org/mediawiki/2013/0/02/Sponsorship.png" style="height:90px;" /><br />
<a href="https://igem.org/Main_Page"><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="rose">QGEM 2013 </span><span class="black">Sponsors</span></div><br />
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Queen's iGEM Team would like to thank all of our generous sponsors!<br />
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<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.queensu.ca/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Queen's University</span></a><br />
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We graciously thank the Department of Chemical Engineering, the Department of Biomedical and Molecular Sciences, the Department of Life Sciences and Biochemistry, the Department of Arts and Science, and the Department of Biology for their generous support of our team! In addition, we would like to thank the Faculty of Engineering and Applied Science for their continued support. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/f/f1/Queens_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.pipetpeoplestore.com/index.cfm/fuseaction/home.home/index.htm" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Calibrate Inc.</span></a><br />
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<div class="offset1 span7 content"><br />
Calibrate, Inc. provides pipette calibration, repair, and documentation services to scientific laboratories across North America. The largest and oldest independent pipette calibration and repair service in North America, Calibrate is a leader in quality in the industry and is known for delivering top-notch work and excellent customer service. <br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;" src="https://static.igem.org/mediawiki/2013/c/ca/Calibrate_logo.jpg"/><br />
</div><br />
</div><br />
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<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://qubitsystems.com/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Qubit Systems Inc.</span></a><br />
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<div class="offset1 span7 content"><br />
Qubit Systems Inc. specializes in the design of instrumentation for research and teaching in the biological sciences, with a scope ranging from plant physiology to human respirometry. Their aim is to provide educational establishments, research institutes and companies with innovative, cost-effective equipment for research, teaching, and industrial applications. Qubit also manufactures and sells a broad range of gas analyzers, environmental sensors, instruments for aquatic biology, and the world's most sophisticated range of fluorescence imaging systems for chlorophyll, GFP and other biologically important compounds.<br />
</div><br />
<div class="span4"><br />
<img style="height:150px;text-align:center;margin-left:30px;" src="https://static.igem.org/mediawiki/2013/6/60/Qubit_logo.jpg"/><br />
</div><br />
</div><br />
<br />
<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:50px;"><br />
<a href ="http://www.conocophillips.ca/EN/Pages/index.aspx" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">ConocoPhillips</span></a><br />
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<div class="offset1 span7 content"><br />
ConocoPhillips is a multinational energy corporation that explores for, produces, transports and markets crude oil, natural gas, natural gas liquids, liquefied natural gas and bitumen on a worldwide basis - energy that plays a foundational role in enabling global economic development and human progress. <br />
</div><br />
<div class="span4"><br />
<img style="height:100px;text-align:center;" src="https://static.igem.org/mediawiki/2013/5/5b/Conocophillips_logo.jpg"/><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Interested in </span><span class="rose">Sponsoring?</span></div><br />
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<div class="offset1 span10 content"><br />
Here at QGEM, we have a lot of passion for what we do. The tragedy though is that passion isn't exchangeable for reagents, which is something that we also need an abundance of. If you would like to show your support for our cause and sponsor us, download the sponsorship package and see what exactly it entails!<br />
</div> <br />
</div><br />
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<div class="row-fluid"><br />
<div class="span12" style="text-align:center;"><br />
<a href ="https://dl.dropboxusercontent.com/u/125588631/QGEM-FINAL.pdf"><br />
<img src="https://dl.dropboxusercontent.com/u/125588631/download.png" style="height:200px; float:center;"/><br />
</a><br />
</div><br />
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<div class="span12" style="vertical-align:middle;text-align:center;"><br />
<h4>Money can't buy us love but it can buy us lab equipment.</h4><br />
<h5>Click here to download the sponsorship package in pdf format! We promise it's nifty.</h5><br />
</div><br />
</div><br />
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<div class="row-fluid"><br />
<div class="offset1 span10 content" style="margin-top:40px;margin-bottom:40px;"><br />
If you have any questions or inquiries about the sponsorship package or potential benefits, don't hesitate to email our project manager Henry Barron at henry.barron@qgemteam.com. <br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Keeping People </span><span class="rose">Safe</span></div><br />
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<div class="offset1 span10 content"><br />
Each team member on the Queen's iGEM 2013 team has received extensive lab safety and Workplace Hazardous Materials Information System (WHMIS) training as several hazardous compounds were encountered. These include ethidium bromide, a potential carcinogen and mutagen, and sodium azide, a potentially deadly chemical. Ethidium bromide was used to run gel electrophoresis while sodium azide was used in several mini chemotaxis assays to paralyze <i>Caenorhabditis elegans</i>. Proper protocols were followed in handling both chemicals, including the use of appropriate safety equipment and proper disposal techniques. As the research conducted this year is considered Level 1 work, all iGEM students worked in an officially approved Level 1 Biohazard Lab. All Canadian national biosafety standards and guidelines were followed. Furthermore, the project idea, methods and safety considerations were reviewed by two faculty advisors, Dr. Kenton Ko and Dr. Ian Chin-Sang, who are both members of the Biohazard Committee at Queen's University. <br />
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</div><br />
<div class="row-fluid" style="margin-top:30px;"><br />
<div class="offset1 span10 content"><br />
The bacterial strains used in the research are non-pathogenic <i>E. coli</i> strains, including K-12, XL1-Blue, and BL-21. These are rated as Biosafety Level 1 and do not pose a threat to laboratory workers, the general public or the environment. All measures outlined in the Material Safety Data Sheets (MSDS) and the biosafety regulations present at Queen's University were followed. These measures prevent our genetically modified E. coli strains from being introduced into the environment, and from posing any risk to security through malicious misuse by individuals, groups, or countries. <br />
</div> <br />
</div><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Application </span><span class="rose">Risks</span></div><br />
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When changing foot odor into a compound that smells like bananas, it could have unpredictable effects such as luring insects or triggering allergic reactions. In order for this to become a commercial product, extensive testing with actual mosquitoes in regular outdoors environments as well as testing its effects on humans would be required. Since the product would require placing bacteria relatively close to human skin, such as the feet, precautions would need to be taken to ensure that the bacteria does not escape the product and colonize the shoes or feet instead. Further testing would be required to see if this would cause adverse effects.<br />
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When working with the mosquito traps, they would have to be at optimal distance from human residences. If they are too far, then the impact would not be as great, but if they are too close, it may lure more mosquitoes to humans. With the idea and knowledge of making mosquito traps, people might get the idea of luring mosquitoes into traps and killing them. This could possibly cause the extinction of mosquitoes, which can potentially have adverse effects on the ecosystem.<br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Keeping People </span><span class="rose">Safe</span></div><br />
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<div class="offset1 span10 content"><br />
Each team member on the Queen's iGEM 2013 team has received extensive lab safety and Workplace Hazardous Materials Information System (WHMIS) training as several hazardous compounds were encountered. These include ethidium bromide, a potential carcinogen and mutagen, and sodium azide, a potentially deadly chemical. Ethidium bromide was used to run gel electrophoresis while sodium azide was used in several mini chemotaxis assays to paralyze <i>Caenorhabditis elegans</i>. Proper protocols were followed in handling both chemicals, including the use of appropriate safety equipment and proper disposal techniques. As the research conducted this year is considered Level 1 work, all iGEM students worked in an officially approved Level 1 Biohazard Lab. All Canadian national biosafety standards and guidelines were followed. Furthermore, the project idea, methods and safety considerations were reviewed by two faculty advisors, Dr. Kenton Ko and Dr. Ian Chin-Sang, who are both members of the Biohazard Committee at Queen's University. <br />
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<div class="offset1 span10 content"><br />
The bacterial strains used in the research are non-pathogenic <i>E. coli</i> strains, including K-12, XL1-Blue, and BL-21. These are rated as Biosafety Level 1 and do not pose a threat to laboratory workers, the general public or the environment. All measures outlined in the Material Safety Data Sheets (MSDS) and the biosafety regulations present at Queen's University were followed. These measures prevent our genetically modified E. coli strains from being introduced into the environment, and from posing any risk to security through malicious misuse by individuals, groups, or countries. <br />
</div> <br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><span class="black">Application </span><span class="rose">Risks</span></div><br />
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<div class="offset1 span10 content"><br />
When changing foot odor into a compound that smells like bananas, it could have unpredictable effects such as luring insects or triggering allergic reactions. In order for this to become a commercial product, extensive testing with actual mosquitoes in regular outdoors environments as well as testing its effects on humans would be required. Since the product would require placing bacteria relatively close to human skin, such as the feet, precautions would need to be taken to ensure that the bacteria does not escape the product and colonize the shoes or feet instead. Further testing would be required to see if this would cause adverse effects.<br />
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<div class="offset1 span10 content"><br />
When working with the mosquito traps, they would have to be at optimal distance from human residences. If they are too far, then the impact would not be as great, but if they are too close, it may lure more mosquitoes to humans. With the idea and knowledge of making mosquito traps, people might get the idea of luring mosquitoes into traps and killing them. This could possibly cause the extinction of mosquitoes, which can potentially have adverse effects on the ecosystem.<br />
</div><br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T04:54:51Z<p>0sec8: </p>
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<img src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png" class="img-circle" style="height:60px;min-width:60px;float:right;"></a><br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The YjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we used YjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
</div><br />
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<br />
<div class="row-fluid" style="margin-top:30px;"><br />
<div class="offset1 span10 content"><br />
Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, YjgB is thought to play a very important role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
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</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/PartsTeam:Queens Canada/Parts2013-09-27T04:54:38Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
<li id="notebook"><a href="https://2013.igem.org/Team:Queens_Canada/Notebook">NOTEBOOK</a></li><br />
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<span class="black">This Year's </span><span class="rose">Part</span><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:0px;"><br />
<div class="offset1 span10 content"><br />
The YjgB enzyme is a medium-chain (1020 bp), zinc-containing enzyme and is part of the dehydrogenase/reductase family. For our project, we used YjgB to reduce isoamyl aldehyde to isoamyl alcohol in order to eventually produce isoamyl acetate. <br />
</div><br />
</div><br />
<br />
<br />
<div class="row-fluid" style="margin-top:30px;"><br />
<div class="offset1 span10 content"><br />
Apart from playing a role in the conversion of isovaleric acid to isoamyl acetate, YjgB is thought to play a very important role in metabolic engineering techniques. Much of today's research in the chemical industry focuses on how to produce fuels from biomass. This means that different microorganisms must be enabled to produce certain types of alcohols, such as ethanol, isobutanol, and n-butanol and the last step in this process involves <br />
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684718/" style="color:#bf3f3e">conversion from an aldehyde to an alcohol</a>. <br />
This is precisely where we believe our yjgB part will prove to be valuable.<br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top:30px;margin-bottom:30px"><br />
<div class="offset1 span10 content"><br />
To find our part on the registry, please follow this link: <br />
<a href="http://parts.igem.org/Part:BBa_K1121000" style="color:#bf3f3e">BBa_K1121000</a>.<br />
</div><br />
</div><br />
<br />
</body><br />
</html></div>0sec8http://2013.igem.org/Team:Queens_Canada/OutreachTeam:Queens Canada/Outreach2013-09-27T04:53:58Z<p>0sec8: </p>
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QGEM 2013 PRESENTS:<br />
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<li id="judging"><a href="https://2013.igem.org/Team:Queens_Canada/Judging">JUDGING</a></li><br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="rose">Spreading </span><span class="black">Synthetic Biology</span><br />
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<div class="offset1 span10"><br />
<a href ="https://2013.igem.org/Team:Queens_Canada/Outreach/ShadValley" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Shad Valley</span></a><br />
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<div class="offset1 span10 content"><br />
This year QGEM collaborated with Shad Valley Queen’s to create a workshop in the field of synthetic biology for high school students. Our goal for this outreach initiative was to expose secondary school students to the emerging field of synthetic biology and spark an interest in this new technology. We tried to emulate the iGEM experience and with these ideas in mind planned a two day crash course. To read more about our Shad Valley workshop click <a href= "https://2013.igem.org/Team:Queens_Canada/Outreach/ShadValley">here</a>.<br />
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<div class="row-fluid" style="margin-top:30px;margin-left:250px;"><br />
<div class="offset1 span5"><br />
<iframe style="border: 10px solid black" width="560" height="315" src="//www.youtube.com/embed/nZU1DZISkp8" frameborder="0" allowfullscreen></iframe><br />
</div><br />
</div><br />
<br />
<div class="row-fluid" style="margin-top: 40px;"><br />
<div class="offset1 span10"><br />
<a href ="http://www.queensinnovation.ca/Explore/About-the-QIC.html" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">Queen's Innovation Connector</span></a><br />
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<div class="offset1 span10 content"><br />
The Queen's Innovation Connector (QIC) is a joint program by the Faculty of Applied Science and School of Business. They aim to encourage, support, and create a platform for aspiring students, professors, and Canadian companies to collaborate through workshops, internship opportunities and programs. This summer they ran a summer program called the Queen's Summer Innovation Initiative (QSII), where Queen's students spend the summer learning about entrepreneurship, corporate innovation and change management from faculty, alumni, entrepreneurs and business experts. The students formed small groups and over the course of four months, started their own businesses or worked in collaboration with a company, culminating in a 10,000 dollar grand prize to the most successful group. <br />
<br><br><br />
Our goal in working with QIC was to build ties with innovative commerce and engineering students. This allows people who normally are not exposed to the field of synthetic biology to learn about the potential of this new technology. In the spirit of collaboration, QGEM gave a thirty minute talk to the members of QSII, explaining the fundamentals of synthetic biology and our project this year. We hope to make QGEM more interdisciplinary in the future to provide our team with a stronger financial base and to inspire QSII members to work in synthetic biology.<br />
</div><br />
</div><br />
<br />
<center><img src = "http://www.queensinnovation.ca/shell/logo-qic.gif" /></center><br />
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<div class="offset1 span10"><br />
<a href ="http://qsynbio.wordpress.com/" style="font-size:0.5em;margin-bottom:20px;"><span class="rose">QSynBio</span></a><br />
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<div class="offset1 span10 content"><br />
The Queen's Synthetic Biology Organization (QSynBio) is a club that aims to foster growth and expansion of the synthetic biology community at Queen's University. Its purpose is to act as an open forum to learn about the field and to develop the necessary molecular biology knowledge and techniques. This year, we are changing the structure of QSynBio so that it ties in more closely with QGEM. This allows for more prepared members and ultimately more time and resources to spend on the project itself. We are excited about the new direction that this collaboration will take us and hope to build a home for synthetic biology at Queen's in the upcoming year. <br />
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<div class="offset1 span10" style="margin-top:40px;margin-bottom:40px;"><br />
<span class="black">Cultivating </span><span class="rose">Media</span><br />
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<div class="offset1 span10 content" style="margin-bottom:30px;"><br />
We have also reached out to different sources concerning our project and spread awareness of what we have been up to. In addition to appearing in the Kingston Whig and the Queen's Journal, we were also contacted by Ontario Morning, a CBC radio show who interviewed our project manager, Henry Barron. Click below to have a look at them!<br />
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<div class="offset1 span6 content"><br />
Click<br />
<a href="http://www.thewhig.com/2013/07/02/foot-odour-key-to-malaria-battle" style="font-color:#320a00;font-size:1.2em;text-decoration:none;">here</a><br />
to read the Kingston Whig article!<br />
</div><br />
</div><br />
<div class="row-fluid"><br />
<div class="offset1 span6 content"><br />
Click<br />
<a href="http://queensjournal.ca/story/2013-07-30/news/student-science-group-sets-malaria-trap/?flavour=mobile" style="font-color:#320a00;font-size:1.2em;text-decoration:none;">here</a><br />
to read the Queen's Journal article!<br />
</div><br />
</div><br />
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