http://2013.igem.org/wiki/index.php?title=Special:Contributions/A&feed=atom&limit=50&target=A&year=&month=2013.igem.org - User contributions [en]2024-03-29T11:04:43ZFrom 2013.igem.orgMediaWiki 1.16.5http://2013.igem.org/Team:Warsaw/TeamTeam:Warsaw/Team2013-10-05T02:38:41Z<p>A: </p>
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<div>{{:Team:Warsaw/Templates/StandardPageBegin|Our team}}<br />
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<!-- <p><b>Our team</b><hr><br/> --><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/54/TeamWarsaw.jpg" border="1" width="650" alt="Team" style="float: left;border:none;margin-right:15px"/><br />
We are proud to present You, our team with our dinosaur!!! This is the mascot of our <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>. As you can see he is on vacation, surfing in our halls in the night, when no one can see him...<br />
<p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in <a href="https://igem.org/Main_Page" target="_blank">IGEM competition</a> from the perspective of the wet lab, which is a completely new experience for them !!! <br />
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Our group is divided into two different laboratories, one is situated in the <a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a> and our second laboratory is situated in the <a href="http://www.igib.uw.edu.pl/index.php?id=80” target="_blank">Institute of Genetics and Biotechnology</a>.<br />
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<p><b>STUDENTS</b><hr><hr><br/><br />
<p><b><a href="mailto:helinhel@gmail.com" target="_blank">Aleksandra Bartosik</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/9/9c/Ola_Bartosik.JPG" width="165" alt="Ola" style="float: left;border:none;margin-right:15px"/><br />
I am studying biotechnology and chemistry at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. I'm fascinated by biomimicry and bioengineering, because we are surrounded with plenty of awe-inspiring technologies almost ready to use. <br />
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My other passion is journalism, learning languages and traveling. In my free time I dive, preferably somewhere in Northern Europe, talk about graphics with my best friend and wonder why the world works the way it does...<br />
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This is my first iGEM competition, so everything is exciting and I can't wait for the Jamboree. In our iGEM Warsaw Team I take care of the Human Practice part of the project and work in the Cell Lab. <br />
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<p><b>Filip Hoffmann</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/1/1b/Igem_wiki.jpg" width="165" alt="Filip" style="float: left;border:none;margin-right:15px"/><br />
I am a student of Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>, I also study Neuroinformatics at the <a href="http://www.fuw.edu.pl/" target="_blank"> Faculty of Physics</a> in our university. My area of interests are genetics of bacteria, and brain - computer interfaces. Privately I am an avid sailor and an amateur football journalist. I also own two lovely cockatiels, Athen and Tyrion.<br />
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<p><b>Elżbieta Jankowska</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/f/ff/262x329.png" width="165" alt="Ela" style="float: left;border:none;margin-right:15px"/><br />
I'm a final year student of Biotechnology, which means I'm currently working on my Masters thesis. Hopefully it will be finished soon. My area of scientific interest includes ancient DNA, molecular phylogenetics, synthetic biology (of course:p) and anything I find interesting at the moment. Apart from that I like traveling, singing and sci-fi & fantasy. <br />
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<p><b>Ewelina Kośmider</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/6/68/As.png" width="165" alt="Ewelina" style="float: left;border:none;margin-right:15px"/><br />
Primarily I'd started studying Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> but after one year I moved to the<a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. In my future I want to be connected with either life science or computer science. The area of my interest is pretty wide: starting from computer security and molecular biology through Chinese and ending up with dancing with fire. <br />
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<p><b><a href="mailto:a.v.kotrys@gmail.com" target="_blank">Anna Kotrys</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/e/e0/AnnaKotrys.jpg" width="165" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
I am a sophomore student of biotechnology at the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>. The area of my scientific interest focuses on alternative splicing and RNA metabolism. What I am also interested in, are molecular mechanisms of cancerogenesis. In 2013 iGEM project I am responsible for the cytotoxicity study in the cell biology part. In the future I would like to follow a scientific pathway which I assume would lead me to a phD in molecular biology. <br />
Apart from biology, I am keen on fencing and tennis and I do love the arts. <br />
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<p><b><a href="mailto:annamiscicka@gmail.com" target="_blank">Anna Miścicka</a></b><hr><br/><br />
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<img src="https://static.igem.org/mediawiki/2013/f/fb/S1051505.JPG" width="165" alt="Mysz" style="float: left;border:none;margin-right:15px"/><br />
I am studing biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a><br />
of the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>. This year I did my Bachelor thesis in the <br />
<a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a>, so next year I'll start Master thesis, also in the <br />
<a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a>.<br/><br />
I have an artistic nature. I love drawing and singing. I am a soprano in Faculty's Choir since January 2012. In free time I am watching animated films (Disney, Pixar, DreamWorks and this stuff) - I am partial to polish dubbing.<br/><br />
I think if I didn't chose science, I would be a grafic artist, singer or voice actress.<br />
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<p><b>Aleksandra Olszewska</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/52/OlkaOlszewska.jpg" width="165" alt="Ola" style="float: left;border:none;margin-right:15px"/><br />
I am a student of graphic design at the <a href="http://www.asp.waw.pl/" target="_blank"> Academy of Fine Arts</a> in Warsaw. I try to make the work of IGEM Warsaw team more attractive to your eyes...<br />
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<p><b><a href="mailto:dorota.sabat@gmail.com" target="_blank">Dorota Kaja Sabat</a></b><hr><br/><br />
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<img src="https://static.igem.org/mediawiki/2012/1/16/Kaja.png" width="165" alt="Kaja" style="float: left;border:none;margin-right:15px"/><br />
I am studying biology and psychology at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. So far I was researching mutations in POLG1 human gene, but this year I am beginning my adventure with embryology. Aside from science, I like traveling and taking photographs. I am also enthusiastic rock climber :)<br />
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<p><b><a href="mailto:anna.saffray@gmail.com" target="_blank">Anna Saffray</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/b/bc/AnnaSaffray.jpg" width="165" height="170" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
I am a first year student at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> of our university and I am studying biotechnology.<br />
<br/>I enjoy spending time in the laboratory learning new exciting approaches, methods and facts, that is why I am trying different projects. I have met the World of: plants, bacteria, yeasts, as well as the World of cells, I don't know yet what to choose but I guess at one point I will just know.<br />
<br/><br/>I can't wait to go to the IGEM conference and hear about new ideas and projects of other students that are going to be present, it is always a great opportunity to talk with people that share the same passion for science.<br />
<br/>Apart from my first hobby (science) I enjoy listening to classical music,<br />
especially <a href="http://en.wikipedia.org/wiki/Sergei_Rachmaninoff" target="_blank">Sergei Rachmaninoff</a> and play the piano. <br/> When I get away from the crowd in Warsaw I enjoy the wonder of <a href="http://en.wikipedia.org/wiki/Natural_horsemanship/" target="_blank"> Natural Horsemanship</a>. Spending time with my horses taught me that all I need to do is: be present, listen, observe and everything will be alright... <br />
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<p><b><a href="mailto:kasia.sitarz93@gmail.com" target="_blank">Katarzyna Sitarz</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/7/72/KatarzynaSitarz.JPG" width="165" height="200" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
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I am studying biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>. I am fascinated by biology since middle school. My main subjects of interest are: Genetics, virology, and carcinogenesis.</br> I have so many ideas about my future as a scientist that I have no idea with which area of biology I will spend my time on... <br />
</br> Apart science I am interested in politics, feminism and recently I became also interested in fun facts about the subway... </br> I am befriended with a cat named Richard...<br />
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<p><b><a href="mailto:roza.weglinska@gmail.com" target="_blank">Róża Węglińska</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/4/4a/R%C3%B3%C5%BCa_%C5%82o%C5%9B_Pilchy.jpg" width="165" height="200" alt="Róża" style="float: left;border:none;margin-right:15px"/><br />
</br><br />
I am studying biotechnology and psychology at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science </a> .<br />
I am particularly interested in genetics, molecular biology and proteomics, as well as neurobiology and neurogenetics.</br> This year I did my Bachelor thesis in the <a href="http://www.igib.uw.edu.pl/index.php?id=80” target="_blank">Institute of Genetics and Biotechnology</a><br />
in the <br />
<a href="http://adz.ibb.waw.pl " target="_blank"> Laboratory of RNA Biology and Functional Genomics </a>. Next year I'll start Master thesis, also in this lab, carrying out a project related to the study of protein interactions connected to CCR4-NOT complex in Homo sapiens. </br> Since May 2011 I am the head of the <a href="http://epigen.arabidopsis.pl/" target="_blank"> Student Genetics and Epigenetics Society </a> at the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>.</br><br />
I'm fascinated by learning and teaching, dealing with new teaching developments in early childhood education. Besides, I like traveling, reading books, playing board games and singing (I am a soprano at the<a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> Choir since October 2010). I am also collecting postcards and tea from all around the world...<br />
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<p><b><a href="mailto:kamilzmijewski95@gmail.com" target="_blank">Kamil Żmijewski</a></b><hr><br/><br />
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<img src="https://static.igem.org/mediawiki/igem.org/0/02/F10030656.jpg" width="165" alt="Kamil" style="float: left;border:none;margin-right:15px"/><br />
I am a student of the <a href="http://batory.edu.pl/strona/IB" target="_blank"> International Baccalaureate Diploma Programme at the Stefan Batory High School</a> in Warsaw. In the project, I am working in the cell biology section, taking part in the <a href="https://2013.igem.org/Team:Warsaw/Cytotoxicity" target="_blank">measurements of cytotoxicity</a> of acrylamide in eukaryotic cell lines. The work in the laboratory is an engaging and rewarding experience, which is complemented by excellent supervision and cooperation. Apart from a strong interest in science and health-related issues, I am also interested in photography and semantics. I wish to continue education in medicine or a related area leading to clinical practice and non-clinical medical research.<br />
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<p><b>ADVISORS</b><hr/><hr/><br/><br />
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<p><b>Kamil Koziara</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/9/9e/Pacz_pusto.png" width="165" alt="Kamil" style="float: left;border:none;margin-right:15px"/><br />
I am studying Computer Science and Biotechnology at the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>, but that's just on paper. In reality I am trying to get more holistic view of the world which basically means running around hectically, learning random stuff to answer intriguing me questions to get even more questions. It's a vicious circle, but it's really fun to do so.<br />
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Recently I am joining the hype over the big data and bioinformatics. I am fascinated by bioengineering and its applications.<br />
I love to read. From time to time I torture myself with some philosophical concepts. When I am too tired to think I go for a swim.<br />
<span style="color:white;">\(^-^)/</span><br />
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<p><b>Anna Misiukiewicz</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/3/3d/20120925201352!2.jpg" width="165" alt="Anna" style="float: left;border:none;margin-right:15px"/><br />
I am a graduate of Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">University of Warsaw</a> with a specialization in microbiology. The end of my studies couldn’t hold me back from continuing my adventure with science, that’s why I’m a Warsaw Team member. Now I’m mainly focused on the field of my employment – a quality control in pharmaceutical production which I find very interesting and quite different from anything I've done so far. I still can’t imagine my life without a cup of black tea, weather forecast and visiting my hometown – <a href="http://en.wikipedia.org/wiki/Bia%C5%82ystok" target="_blank"> Białystok</a>. As often as possible I spend my free time with my great hobbies - psychology and analogue photography.<br />
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<p><b>Mikołaj Ogrodnik</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/3/38/Miko%C5%82ajOgrodnik.jpg" width="165" height="200" style="float: left;border:none;margin-right:15px"/><br />
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I have finished my master studies at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a><br />
this year and from October on I will continue as a PhD student. I am highly interested in cellular biology, mostly in processes concerning ageing and senescence. I believe that the better understanding of ageing process could provide the means to better treat age-related diseases and even to extend human lifespan.<br />
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I like also botany, molecular biology and bioinformatics. I enjoy traveling and doing small (or sometimes bigger) scientific projects all around the world. Outside the lab I am spending my time on long strolls, reading books and rock climbing.</p><br clear="all"/><br clear="all"/><br />
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<p><b><a href="mailto:szczepaniak.krzysiek@gmail.com" target="_blank"> Krzysztof Szczepaniak</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/4/4b/Krzysiek.jpg" width="165" height="200" alt="Krzysiek" style="float: left;border:none;margin-right:15px"/><br />
I have graduated from the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a> and now I'm beginning my PhD studies at the <br />
<a href="http://www.iimcb.gov.pl/ target="_blank">International Institute of Molecular and Cell Biology in Warsaw</a>.<br />
Beside biology, I like computer programming and I<br />
try to develop my skills in this field. Outside the lab I am a scout<br />
leader, I am interested in foreign languages, astronomy and I love<br />
everything connected with science-fiction and fantasy.</p><br clear="all"/><br clear="all"/><br />
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<p><b>Antoni Rościszewski</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/55/AntoniRosciszewski.jpg" width="165" height="206" alt="Antoni" style="float: left;border:none;margin-right:15px"/><br />
I am an undergraduate bioinformatics student at the University of Warsaw (<a href="http://www.mimuw.edu.pl/?LANG=en" target="_blank">Faculty of Mathematics, Informatics and Mechanics</a>. I'm particularly interested in systems biology and bioinformatics applications in synthetic biology, as well as personal genomics. <br clear="all"/><br clear="all"/><br />
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<p><b>Marcin Ziemniak</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/1/1a/Marcin_Ziemniak.jpg" width="165" height="165" alt="Marcin Ziemniak" style="float: left;border:none;margin-right:15px"/><br />
I have graduated from the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a> (M.S in bioorganic chemistry and B.S in molecular biology). Currently, I am a PhD student at the <a href="http://www.fuw.edu.pl/faculty-of-physics-home.html" target="_blank">Faculty of Physics, University of Warsaw </a>. The scientific project in which I am involved is interdisciplinary, hence in my research I employ not only chemical synthesis of modified nucleotides but also a variety of biochemical and biophysical techniques. I am also quite interested in structural biology and nanotechnology. Apart from academia I enjoy travelling, taking photos and sport. I am also an avid fan of science fiction and fantasy.<br />
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<p><b>INSTRUCTORS</b><hr><hr/><br/><br />
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<p><b>prof. dr hab. Jacek Bielecki</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2008/1/1c/Prof.Jacek_Bielecki.gif" width="165" alt="prof. Bielecki" style="float: left;border:none;margin-right:15px"/><br />
<br>Education: MSc, <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>, 1975; PhD, <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>, 1981; Associated professor, Warsaw University, 1995; Professor at <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a><br />
, 1996; Vice Dean of <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>, 1996 - 1999, and 1999-2002<br />
<br>Research interests:<br />
Molecular mechanisms of virulence of bacteria <i>Listeria monocytogenes</i>, especially the role of a hemolysin, listeriolysin O (LLO).<br />
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<p><b>dr Takao Ishikawa</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/f/fb/TI.png" width="165" alt="Takao" style="float: left;border:none;margin-right:15px"/><br />
Born in <br />
<a href="http://en.wikipedia.org/wiki/Tokyo" target="_blank"> Tokyo</a>, working and living in <a href="http://en.wikipedia.org/wiki/Warsaw" target="_blank"> Warsaw</a>, I am interested in<br />
protein-protein interactions. In my research, I employ in silico<br />
modeling of protein complexes and theirs verification by molecular<br />
biology methods. I am not only fascinated by life science, but also<br />
being in love with popularization of science. Giving lectures for<br />
students, teachers, and children give me a lot of fun!</p><br clear="all"/><br clear="all"/><br />
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<p><b><a href="mailto:q.piatkowski@gmail.com" target="_blank">mgr Jakub Piątkowski </a></b><hr><br/><br />
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<img src="https://static.igem.org/mediawiki/2009/7/73/Portret.jpg" width="165" alt="Radek" style="float: left;border:none;margin-right:15px"/><br />
I am a molecular biology Ph.D. student at the University of Warsaw <a href="http://www.igib.uw.edu.pl/index.php?id=80 target="_blank">Institute of Genetics and Biotechnology</a>. My research interests include RNA turnover in mitochondria and the evolution of organellar genomes.</p><br clear="all"/><br clear="all"/><br />
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<p><b><a href="mailto:rstachowiak76@gmail.com" target="_blank">dr Radosław Stachowiak </a></b><hr><br/><br />
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<img src="https://static.igem.org/mediawiki/2009/6/69/RadoslawStachowiak.jpg" width="165" alt="Radek" style="float: left;border:none;margin-right:15px"/><br />
My adventure with biology and biotechnology dates back to 1995 when I became a biotechnology student at the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>. In the year 2000 I completed my MSc thesis and I guess I couldn’t stop there so I immediately started PhD studies. Now I’m done with studying and my job is to take care of students and introduce them into science. Still, I like being involved in crazy projects so characteristic of Sudent's Societies. The more crazy and incredible project the better. I guess this is the case so here I am.<br />
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<p><b>dr Roman Szczęsny</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/5b/RomanSzczesny.jpg" width="165" alt="Roman Szczęsny" style="float: left;border:none;margin-right:15px"/><br />
My principal research interests lie in the field of RNA metabolism. I am especially interested in RNA processing, degradation and quality control pathways executed in different compartments of human cells. I enjoy sharing my experience and know-how with highly motivated students.</p><a name="acknowledgments">&nbsp;</a><br />
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<p><h4><a href="https://2013.igem.org/Team:Warsaw/Acknowledgments">Click here for full our 2013 project acknowledgments.</a></h4></p><br />
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p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in<br />
p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in<br />
p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in</div>Ahttp://2013.igem.org/Team:Warsaw/Templates/NavigationBarTeam:Warsaw/Templates/NavigationBar2013-10-05T02:02:10Z<p>A: </p>
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<li><a href="/Team:Warsaw/The night of Biologists">The Night of Biologists</a></li><br />
<li><a href="/Team:Warsaw/Science_Picnic_2013">Science Picnic 2013</a></li><br />
<li><a href="/Team:Warsaw/Our cooperation with mass media">Our cooperation with mass media </a></li><br />
</ul><br />
</li><br />
<li class="navitem4"><br />
<a href="/Team:Warsaw/Extras" title="">Extras</a><br />
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<li><a href="/Team:Warsaw/Genetic_lab_journal">Genetic lab journal</a></li><br />
<li><a href="/Team:Warsaw/Cellular_biology_lab_journal">Cellular biology lab journal</a></li><br />
<li><a href="/Team:Warsaw/Glossary">Project glossary</a></li><br />
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<li><a href="/Team:Warsaw/Team">Team</a></li><br />
<li><a href="/Team:Warsaw/Acknowledgments">Acknowledgments</a></li><br />
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<br />
</html></div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:43:06Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Acrylamide detection==<br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
==BiFC Toolbox==<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Shyu, Y. J., Liu, H., Deng, X., Hu, C. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. BioTechniques 40:61-66 (2006).<br />
<br />
==Cytotoxicity==<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:42:56Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
==BiFC Toolbox==<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Shyu, Y. J., Liu, H., Deng, X., Hu, C. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. BioTechniques 40:61-66 (2006).<br />
<br />
==Cytotoxicity==<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:42:39Z<p>A: /* BiFC Toolbox */</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
<br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
==BiFC Toolbox==<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Shyu, Y. J., Liu, H., Deng, X., Hu, C. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. BioTechniques 40:61-66 (2006).<br />
<br />
==Cytotoxicity==<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/BiFC_ToolboxTeam:Warsaw/BiFC Toolbox2013-10-04T23:42:33Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|BiFC Toolbox}}<br />
__NOTOC__<br />
==Genetic part – The BiFC Toolbox==<br />
<br />
GFP is the most popular fluorescent protein. In 2006 Pédelacq ''et al.'' engineered and characterized new form of GFP – superfolder GFP (sfGFP), which is more stable and has stronger fluorescence than wild form GFP. We aimed to create other superfolder fluorescent proteins and we succeeded.<br />
Changing color of fluorescent proteins is possible using direct mutagenesis technique. We did it using PCR, with [http://parts.igem.org/Part:BBa_I746908 sfGFP] from Parts Registry (BBa_I746908) as a template.<br />
<br />
* [http://parts.igem.org/Part:BBa_K1093000 sfBFP] (K1093000) - blue fluorescence, contains Y66H point mutation compared to the original sfGFP<br />
* [http://parts.igem.org/Part:BBa_K1093001 sfYFP] (K1093001) - yellow fluorescence, contains T203Y point mutation compared to the original sfGFP<br />
* [http://parts.igem.org/Part:BBa_K1093002 sfCFP] (K1093002) - cyan fluorescence, contains Y66W point mutation compared to the original sfGFP<br />
<br />
By doing this we improved sfGFP (by expanding the range of possible excitation/emission optima). One could say that we improved standard forms of CFP, BFP and YFP by creating their superfolder forms. We sent '''sfBFP''', '''sfYFP''' and '''sfCFP''' to Parts Registry.<br />
<br />
When we confirmed the correctness of our constructs by sequencing, we cloned them on pSB1A3 plasmid, added J23100 promoter and B0034 RBS and subsequently measured them in RF. We chose RF, because our standard medium, LB, displays high internal fluorescence, which makes it impossible to visualize sfBFP in liquid without significant experimental error.<br />
<br />
Excitation/emission maxima for each protein:<br />
* sfGFP – 485/510 nm<br />
* sfBFP – 380/445 nm<br />
* sfCFP – 425/475 nm<br />
* sfYFP – 503/540 nm<br />
<br />
[[File:Superfolderwykres.jpg|center|594px|border|caption]]<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
We compared fluorescence levels to that of sfGFP (the fluorescence intensity is normalized, hence sfGFP has 100% fluorescence). <br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
We found in Parts Registry a yellow fluorescent protein, named [http://parts.igem.org/Part:BBa_K864100 SYFP2] (super yellow fluorescent protein 2; BBa_ K864100). We decided to compare it with our sfYFP. We cloned BBa_K864100 on pSB1A3 plasmid with J23100 promoter and B0034 RBS, and measured it in RF. Excitation/emission maxima: 503/540 nm.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
The fact that fluorescence of sfYFP is not as strong as fluorescence of SYFP2 is slighlty disappointing. However, we sincerely congratulate iGEM2012 Uppsala Team for creating a great yellow fluorescent protein. <br />
Comparison with sfVenus (and sfCerulean) protein which we also designed this year would be most interesting. Unfortunately, the synthetic construct did not reach us in time before wiki freeze.<br />
<br />
We created superfolder BFP, CFP and YFP with the intention of creating a '''“BiFC Toolbox”''' for synthetic biology. BiFC (Bimolecular Fluorescent Complementation) is a method used to validate and visualize protein-protein interactions in living cells. Fragments of fluorescent proteins are fused to proteins that we study and if they interact, functional fluorescent protein is formed and a fluorescent signal is emitted. <br />
Superfolder proteins are perfect for BiFC system due to their improved stability and relatively fast folding kinetics.<br />
<br />
We truncated sfGFP, sfCFP, sfBFP and sfYFP by PCR using specific primers. N-terminal fragment is long (645 bp) and specific to each protein. C-terminal fragment is very short (54 bp) and comes from sfGFP, but we suspect that it would also work well with other fluorescent proteins as well. C-terminus is designed in two variants: m6 and m12 (differing in sensitivity to specificity ratio).<br />
Moreover, we attempted to produce BiFC fragments from [http://parts.igem.org/Part:BBa_E0040 GFP] (E0040), [http://parts.igem.org/Part:BBa_J06504 mCherry] (J06504) and [http://parts.igem.org/Part:BBa_E2050 mOrange] (E2050).<br />
<br />
We successfully amplificated '''N-sfBFP, N-sfYFP, N-sfCFP, N-mCherry, C-mCherry''' and cloned them into pSB1C3 plasmid. We send those parts to Parts Registry. C-m6 and C-m12 fragments, in part due to their small size, proved to be difficult to clone. Thus, we didn’t manage to submit the DNA samples before the deadline. <br />
<br />
To verify the functionality of our BiFC fragments, we decided to use '''b-Fos''' and '''b-Jun''' proteins, which interact with each other. We planned to fuse b-Jun with N-terminal fragment and b-Fos with C-terminal one. Furthermore, we intended to use b-Fos without leucine zipper (in this form it is not able to interact with b-Jun) as a negative control. For measurements we lysed bacteria expressing either b-Fos and b-Jun fused with part of a fluorescent protein and mixed the lysates. This way we will create not only a wide range of BiFC proteins of different parameters, but also provide systematic and standardized information about sensitivity and specificity of each combination. We will also measure Venus- and Cerulean-based BiFC fragments already present in the registry.<br />
<br />
Unfortunately, vacation is over and we did not manage to fulfill our ultimate goal and measure our constructs this year. We will however continue working on the toolbox, as we feel it will prove a valuable addition to the Registry. <br />
<br />
<br />
'''References:''' <br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Shyu, Y. J., Liu, H., Deng, X., Hu, C. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. BioTechniques 40:61-66 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:41:57Z<p>A: /* Acrylamide detection */</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
<br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
==BiFC Toolbox==<br />
<br />
==Cytotoxicity==<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:41:19Z<p>A: /* Cytotoxicity */</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
<br />
==BiFC Toolbox==<br />
<br />
==Cytotoxicity==<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:41:01Z<p>A: /* Cytotoxicity */</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
<br />
==BiFC Toolbox==<br />
<br />
==Cytotoxicity==<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}<br />
<br />
# Stott-Miller M, Neuhouser ML, Stanford JL. Consumption of deep-fried foods and risk of prostate cancer. Epub (2013).<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A. Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes. Epub (2012).<br />
# Besaratinia A and Pfeifer GP. A review of mechanisms of acrylamide carcinogenicity. Carcinogenesis 28, 519–528 (2007).<br />
# O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 28. 5421-6 (2000).<br />
# Koyamaa N, Sakamoto H, Sakuraba M, Koizumi T, Takashima Y, Hayashi M, Matsufuji H, Yamagata K, Masudac S, Kinae S, Honmaa M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutation Research 603, 151–158 (2006).</div>Ahttp://2013.igem.org/Team:Warsaw/TeamTeam:Warsaw/Team2013-10-04T23:39:52Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Our team}}<br />
__TOC__<br />
<html><br />
<div id="team"><br />
<!-- <p><b>Our team</b><hr><br/> --><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/54/TeamWarsaw.jpg" border="1" width="650" alt="Team" style="float: left;border:none;margin-right:15px"/><br />
We are proud to present You, our team with our dinosaur!!! This is the mascot of our <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>. As you can see he is on vacation, surfing in our halls in the night, when no one can see him...<br />
<p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in <a href="https://igem.org/Main_Page" target="_blank">IGEM competition</a> from the perspective of the wet lab, which is a completely new experience for them !!! <br />
<br><br />
<br><br />
<br><br />
Our group is divided into two different laboratories, one is situated in the <a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a> and our second laboratory is situated in the <a href="http://www.igib.uw.edu.pl/index.php?id=80” target="_blank">Institute of Genetics and Biotechnology</a>.<br />
<br />
</p></p><br clear="all"/><br clear="all"/><br />
<p><b>STUDENTS</b><hr><hr><br/><br />
<p><b><a href="mailto:helinhel@gmail.com" target="_blank">Aleksandra Bartosik</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/9/9c/Ola_Bartosik.JPG" width="165" alt="Ola" style="float: left;border:none;margin-right:15px"/><br />
I am studying biotechnology and chemistry at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. I'm fascinated by biomimicry and bioengineering, because we are surrounded with plenty of awe-inspiring technologies almost ready to use. <br />
<br/><br />
My other passion is journalism, learning languages and traveling. In my free time I dive, preferably somewhere in Northern Europe, talk about graphics with my best friend and wonder why the world works the way it does...<br />
<br/><br />
This is my first iGEM competition, so everything is exciting and I can't wait for the Jamboree. In our iGEM Warsaw Team I take care of the Human Practice part of the project and work in the Cell Lab. <br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Filip Hoffmann</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/1/1b/Igem_wiki.jpg" width="165" alt="Filip" style="float: left;border:none;margin-right:15px"/><br />
I am a student of Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>, I also study Neuroinformatics at the <a href="http://www.fuw.edu.pl/" target="_blank"> Faculty of Physics</a> in our university. My area of interests are genetics of bacteria, and brain - computer interfaces. Privately I am an avid sailor and an amateur football journalist. I also own two lovely cockatiels, Athen and Tyrion.<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Elżbieta Jankowska</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/f/ff/262x329.png" width="165" alt="Ela" style="float: left;border:none;margin-right:15px"/><br />
I'm a final year student of Biotechnology, which means I'm currently working on my Masters thesis. Hopefully it will be finished soon. My area of scientific interest includes ancient DNA, molecular phylogenetics, synthetic biology (of course:p) and anything I find interesting at the moment. Apart from that I like traveling, singing and sci-fi & fantasy. <br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Ewelina Kośmider</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/6/68/As.png" width="165" alt="Ewelina" style="float: left;border:none;margin-right:15px"/><br />
Primarily I'd started studying Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> but after one year I moved to the<a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. In my future I want to be connected with either life science or computer science. The area of my interest is pretty wide: starting from computer security and molecular biology through Chinese and ending up with dancing with fire. <br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:a.v.kotrys@gmail.com" target="_blank">Anna Kotrys</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/e/e0/AnnaKotrys.jpg" width="165" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
I am a sophomore student of biotechnology at the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>. The area of my scientific interest focuses on alternative splicing and RNA metabolism. What I am also interested in, are molecular mechanisms of cancerogenesis. In 2013 iGEM project I am responsible for the cytotoxicity study in the cell biology part. In the future I would like to follow a scientific pathway which I assume would lead me to a phD in molecular biology. <br />
Apart from biology, I am keen on fencing and tennis and I do love the arts. <br />
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<br />
<p><b><a href="mailto:annamiscicka@gmail.com" target="_blank">Anna Miścicka</a></b><hr><br/><br />
<br />
<img src="https://static.igem.org/mediawiki/2013/f/fb/S1051505.JPG" width="165" alt="Mysz" style="float: left;border:none;margin-right:15px"/><br />
I am studing biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a><br />
of the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>. This year I did my Bachelor thesis in the <br />
<a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a>, so next year I'll start Master thesis, also in the <br />
<a href="http://www.biol.uw.edu.pl/en/institute-of-biochemistry/department-of-molecular-biology" target="_blank">Department of Molecular Biology</a>.<br/><br />
I have an artistic nature. I love drawing and singing. I am a soprano in Faculty's Choir since January 2012. In free time I am watching animated films (Disney, Pixar, DreamWorks and this stuff) - I am partial to polish dubbing.<br/><br />
I think if I didn't chose science, I would be a grafic artist, singer or voice actress.<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Aleksandra Olszewska</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/52/OlkaOlszewska.jpg" width="165" alt="Ola" style="float: left;border:none;margin-right:15px"/><br />
I am a student of graphic design at the <a href="http://www.asp.waw.pl/" target="_blank"> Academy of Fine Arts</a> in Warsaw. I try to make the work of IGEM Warsaw team more attractive to your eyes...<br />
</p><br clear="all"/><br clear="all"/> <br />
<br />
<br />
<br />
<br />
<br />
<p><b><a href="mailto:dorota.sabat@gmail.com" target="_blank">Dorota Kaja Sabat</a></b><hr><br/><br />
<br />
<img src="https://static.igem.org/mediawiki/2012/1/16/Kaja.png" width="165" alt="Kaja" style="float: left;border:none;margin-right:15px"/><br />
I am studying biology and psychology at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science</a>. So far I was researching mutations in POLG1 human gene, but this year I am beginning my adventure with embryology. Aside from science, I like traveling and taking photographs. I am also enthusiastic rock climber :)<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:anna.saffray@gmail.com" target="_blank">Anna Saffray</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/b/bc/AnnaSaffray.jpg" width="165" height="170" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
I am a first year student at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> of our university and I am studying biotechnology.<br />
<br/>I enjoy spending time in the laboratory learning new exciting approaches, methods and facts, that is why I am trying different projects. I have met the World of: plants, bacteria, yeasts, as well as the World of cells, I don't know yet what to choose but I guess at one point I will just know.<br />
<br/><br/>I can't wait to go to the IGEM conference and hear about new ideas and projects of other students that are going to be present, it is always a great opportunity to talk with people that share the same passion for science.<br />
<br/>Apart from my first hobby (science) I enjoy listening to classical music,<br />
especially <a href="http://en.wikipedia.org/wiki/Sergei_Rachmaninoff" target="_blank">Sergei Rachmaninoff</a> and play the piano. <br/> When I get away from the crowd in Warsaw I enjoy the wonder of <a href="http://en.wikipedia.org/wiki/Natural_horsemanship/" target="_blank"> Natural Horsemanship</a>. Spending time with my horses taught me that all I need to do is: be present, listen, observe and everything will be alright... <br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:kasia.sitarz93@gmail.com" target="_blank">Katarzyna Sitarz</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/7/72/KatarzynaSitarz.JPG" width="165" height="200" alt="Ania" style="float: left;border:none;margin-right:15px"/><br />
<br />
I am studying biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>. I am fascinated by biology since middle school. My main subjects of interest are: Genetics, virology, and carcinogenesis.</br> I have so many ideas about my future as a scientist that I have no idea with which area of biology I will spend my time on... <br />
</br> Apart science I am interested in politics, feminism and recently I became also interested in fun facts about the subway... </br> I am befriended with a cat named Richard...<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:roza.weglinska@gmail.com" target="_blank">Róża Węglińska</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/4/4a/R%C3%B3%C5%BCa_%C5%82o%C5%9B_Pilchy.jpg" width="165" height="200" alt="Róża" style="float: left;border:none;margin-right:15px"/><br />
</br><br />
I am studying biotechnology and psychology at the <a href="http://en.mismap.uw.edu.pl/" target="_blank"> College of Inter-faculty Individual Studies in Mathematics and Natural Science </a> .<br />
I am particularly interested in genetics, molecular biology and proteomics, as well as neurobiology and neurogenetics.</br> This year I did my Bachelor thesis in the <a href="http://www.igib.uw.edu.pl/index.php?id=80” target="_blank">Institute of Genetics and Biotechnology</a><br />
in the <br />
<a href="http://adz.ibb.waw.pl " target="_blank"> Laboratory of RNA Biology and Functional Genomics </a>. Next year I'll start Master thesis, also in this lab, carrying out a project related to the study of protein interactions connected to CCR4-NOT complex in Homo sapiens. </br> Since May 2011 I am the head of the <a href="http://epigen.arabidopsis.pl/" target="_blank"> Student Genetics and Epigenetics Society </a> at the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>.</br><br />
I'm fascinated by learning and teaching, dealing with new teaching developments in early childhood education. Besides, I like traveling, reading books, playing board games and singing (I am a soprano at the<a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a> Choir since October 2010). I am also collecting postcards and tea from all around the world...<br />
</p><br clear="all"/><br clear="all"/><br />
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<br />
<p><b><a href="mailto:kamilzmijewski95@gmail.com" target="_blank">Kamil Żmijewski</a></b><hr><br/><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/igem.org/0/02/F10030656.jpg" width="165" alt="Kamil" style="float: left;border:none;margin-right:15px"/><br />
I am a student of the <a href="http://batory.edu.pl/strona/IB" target="_blank"> International Baccalaureate Diploma Programme at the Stefan Batory High School</a> in Warsaw. In the project, I am working in the cell biology section, taking part in the <a href="https://2013.igem.org/Team:Warsaw/Cytotoxicity" target="_blank">measurements of cytotoxicity</a> of acrylamide in eukaryotic cell lines. The work in the laboratory is an engaging and rewarding experience, which is complemented by excellent supervision and cooperation. Apart from a strong interest in science and health-related issues, I am also interested in photography and semantics. I wish to continue education in medicine or a related area leading to clinical practice and non-clinical medical research.<br />
</p><br clear="all"/><br clear="all"/><br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<br />
<br />
<p><b>ADVISORS</b><hr/><hr/><br/><br />
<br />
<br />
<br />
<br />
<p><b>Kamil Koziara</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/9/9e/Pacz_pusto.png" width="165" alt="Kamil" style="float: left;border:none;margin-right:15px"/><br />
I am studying Computer Science and Biotechnology at the <a href="http://www.uw.edu.pl/en/" target="_blank">University of Warsaw</a>, but that's just on paper. In reality I am trying to get more holistic view of the world which basically means running around hectically, learning random stuff to answer intriguing me questions to get even more questions. It's a vicious circle, but it's really fun to do so.<br />
<br/><br />
Recently I am joining the hype over the big data and bioinformatics. I am fascinated by bioengineering and its applications.<br />
I love to read. From time to time I torture myself with some philosophical concepts. When I am too tired to think I go for a swim.<br />
<span style="color:white;">\(^-^)/</span><br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<br />
<p><b>Anna Misiukiewicz</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/3/3d/20120925201352!2.jpg" width="165" alt="Anna" style="float: left;border:none;margin-right:15px"/><br />
I am a graduate of Biotechnology at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">University of Warsaw</a> with a specialization in microbiology. The end of my studies couldn’t hold me back from continuing my adventure with science, that’s why I’m a Warsaw Team member. Now I’m mainly focused on the field of my employment – a quality control in pharmaceutical production which I find very interesting and quite different from anything I've done so far. I still can’t imagine my life without a cup of black tea, weather forecast and visiting my hometown – <a href="http://en.wikipedia.org/wiki/Bia%C5%82ystok" target="_blank"> Białystok</a>. As often as possible I spend my free time with my great hobbies - psychology and analogue photography.<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Mikołaj Ogrodnik</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/3/38/Miko%C5%82ajOgrodnik.jpg" width="165" height="200" style="float: left;border:none;margin-right:15px"/><br />
<br/><br />
I have finished my master studies at the <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a><br />
this year and from October on I will continue as a PhD student. I am highly interested in cellular biology, mostly in processes concerning ageing and senescence. I believe that the better understanding of ageing process could provide the means to better treat age-related diseases and even to extend human lifespan.<br />
<br/><br />
I like also botany, molecular biology and bioinformatics. I enjoy traveling and doing small (or sometimes bigger) scientific projects all around the world. Outside the lab I am spending my time on long strolls, reading books and rock climbing.</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:szczepaniak.krzysiek@gmail.com" target="_blank"> Krzysztof Szczepaniak</a></b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/4/4b/Krzysiek.jpg" width="165" height="200" alt="Krzysiek" style="float: left;border:none;margin-right:15px"/><br />
I have graduated from the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a> and now I'm beginning my PhD studies at the <br />
<a href="http://www.iimcb.gov.pl/ target="_blank">International Institute of Molecular and Cell Biology in Warsaw</a>.<br />
Beside biology, I like computer programming and I<br />
try to develop my skills in this field. Outside the lab I am a scout<br />
leader, I am interested in foreign languages, astronomy and I love<br />
everything connected with science-fiction and fantasy.</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Antoni Rościszewski</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/55/AntoniRosciszewski.jpg" width="165" height="206" alt="Antoni" style="float: left;border:none;margin-right:15px"/><br />
I am an undergraduate bioinformatics student at the University of Warsaw (<a href="http://www.mimuw.edu.pl/?LANG=en" target="_blank">Faculty of Mathematics, Informatics and Mechanics</a>. <!-- I do love building the apps for smartphones as well as programming some incomprehensible stuff. I am a fervent fan of <a href="http://en.wikipedia.org/wiki/Stanley_Kubrick" target="_blank"> Stanley Kubrick</a> and <a href="http://en.wikipedia.org/wiki/Daniel_Day-Lewis" target="_blank"> Daniel Day-Lewis</a>. I got interested in bio-related issues as I was bored with studying finance at SGH. I must say, I do enjoy lab work. I appreciate especially working in the cell lab. Haze of ethanol favors creativeness as well as establishment of interpersonal relationships. In the future I would like to sink my teeth into medical diagnostics applications of bioinformatics. I would also like to explore outer space. Can't wait to see you all guys at jamboree this year! <3 <p><u>To whom it may concern:</u> Please note that this short description above wasn't in fact written by me (and you will probably know who did this). Although it is quite accurate in some points, I will be very glad to have an opportunity to talk to you about who I am, possibly before September 14.</p>--></p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>Marcin Ziemniak</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/1/1a/Marcin_Ziemniak.jpg" width="165" height="165" alt="Marcin Ziemniak" style="float: left;border:none;margin-right:15px"/><br />
I have graduated from the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a> (M.S in bioorganic chemistry and B.S in molecular biology). Currently, I am a PhD student at the <a href="http://www.fuw.edu.pl/faculty-of-physics-home.html" target="_blank">Faculty of Physics, University of Warsaw </a>. The scientific project in which I am involved is interdisciplinary, hence in my research I employ not only chemical synthesis of modified nucleotides but also a variety of biochemical and biophysical techniques. I am also quite interested in structural biology and nanotechnology. Apart from academia I enjoy travelling, taking photos and sport. I am also an avid fan of science fiction and fantasy.<br />
</p></p><br clear="all"/><br clear="all"/<br />
<br />
<p><b>INSTRUCTORS</b><hr><hr/><br/><br />
<br />
<p><b>prof. dr hab. Jacek Bielecki</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2008/1/1c/Prof.Jacek_Bielecki.gif" width="165" alt="prof. Bielecki" style="float: left;border:none;margin-right:15px"/><br />
<br>Education: MSc, <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>, 1975; PhD, <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>, 1981; Associated professor, Warsaw University, 1995; Professor at <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a><br />
, 1996; Vice Dean of <a href="http://www.biol.uw.edu.pl/en/" target="_blank">Faculty of Biology</a>, 1996 - 1999, and 1999-2002<br />
<br>Research interests:<br />
Molecular mechanisms of virulence of bacteria <i>Listeria monocytogenes</i>, especially the role of a hemolysin, listeriolysin O (LLO).<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>dr Takao Ishikawa</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2012/f/fb/TI.png" width="165" alt="Takao" style="float: left;border:none;margin-right:15px"/><br />
Born in <br />
<a href="http://en.wikipedia.org/wiki/Tokyo" target="_blank"> Tokyo</a>, working and living in <a href="http://en.wikipedia.org/wiki/Warsaw" target="_blank"> Warsaw</a>, I am interested in<br />
protein-protein interactions. In my research, I employ in silico<br />
modeling of protein complexes and theirs verification by molecular<br />
biology methods. I am not only fascinated by life science, but also<br />
being in love with popularization of science. Giving lectures for<br />
students, teachers, and children give me a lot of fun!</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b><a href="mailto:q.piatkowski@gmail.com" target="_blank">mgr Jakub Piątkowski </a></b><hr><br/><br />
<br />
<img src="https://static.igem.org/mediawiki/2009/7/73/Portret.jpg" width="165" alt="Radek" style="float: left;border:none;margin-right:15px"/><br />
I am a molecular biology Ph.D. student at the University of Warsaw <a href="http://www.igib.uw.edu.pl/index.php?id=80 target="_blank">Institute of Genetics and Biotechnology</a>. My research interests include RNA turnover in mitochondria and the evolution of organellar genomes.</p><br clear="all"/><br clear="all"/><br />
<br />
<br />
<p><b><a href="mailto:rstachowiak76@gmail.com" target="_blank">dr Radosław Stachowiak </a></b><hr><br/><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2009/6/69/RadoslawStachowiak.jpg" width="165" alt="Radek" style="float: left;border:none;margin-right:15px"/><br />
My adventure with biology and biotechnology dates back to 1995 when I became a biotechnology student at the <a href="http://www.uw.edu.pl/en/ target="_blank">University of Warsaw</a>. In the year 2000 I completed my MSc thesis and I guess I couldn’t stop there so I immediately started PhD studies. Now I’m done with studying and my job is to take care of students and introduce them into science. Still, I like being involved in crazy projects so characteristic of Sudent's Societies. The more crazy and incredible project the better. I guess this is the case so here I am.<br />
</p><br clear="all"/><br clear="all"/><br />
<br />
<p><b>dr Roman Szczęsny</b><hr><br/><br />
<img src="https://static.igem.org/mediawiki/2013/5/5b/RomanSzczesny.jpg" width="165" alt="Roman Szczęsny" style="float: left;border:none;margin-right:15px"/><br />
My principal research interests lie in the field of RNA metabolism. I am especially interested in RNA processing, degradation and quality control pathways executed in different compartments of human cells. I enjoy sharing my experience and know-how with highly motivated students.</p><a name="acknowledgments">&nbsp;</a><br />
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<br />
<p><h4><a href="https://2013.igem.org/Team:Warsaw/Acknowledgments">Click here for full our 2013 project acknowledgments.</a></h4></p><br />
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{{:Team:Warsaw/Templates/StandardPageEnd}}<br />
p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in<br />
p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in<br />
p>Most of our students are studying biotechnology in our University, but we also have people with Computer Science background that decided to take the challenge and start their adventure in</div>Ahttp://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_referencesTeam:Warsaw/Acknowledgment of sources and references2013-10-04T23:39:32Z<p>A: Created page with "{{:Team:Warsaw/Templates/StandardPageBegin|Judging}} __NOTOC__ ==Acrylamide detection== ==BiFC Toolbox== ==Cytotoxicity== {{:Team:Warsaw/Templates/StandardPageEnd}}"</p>
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<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
<br />
==Acrylamide detection==<br />
<br />
==BiFC Toolbox==<br />
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==Cytotoxicity==<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:38:24Z<p>A: </p>
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<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] [https://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_references Acknowledgment of sources and references]<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] [https://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_references Acknowledgment of sources and references]<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:37:37Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] [https://2013.igem.org/Team:Warsaw/Acknowledgment_of_sources_and_references Acknowledgment of sources and references]<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:36:30Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] [Team:Warsaw/Acknowledgment_of_sources_and_references Acknowledgment_of_sources_and_references]<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
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<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
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<br />
<br />
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<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:35:31Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] [[Acknowledgment of sources and references]]<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:30:47Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:28:07Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:27:40Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:27:09Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
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<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:26:37Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:26:13Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:25:53Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:25:20Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:24:36Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|left|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/File:Sfyfpsyfp2wykres.jpgFile:Sfyfpsyfp2wykres.jpg2013-10-04T23:23:12Z<p>A: uploaded a new version of &quot;File:Sfyfpsyfp2wykres.jpg&quot;</p>
<hr />
<div></div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:21:25Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:21:04Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:19:29Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:19:14Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:18:56Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:18:40Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:18:26Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:18:15Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:18:03Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:17:44Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
<br />
<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:17:19Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|left|594px|border|caption]]<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:16:52Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|center|594px|border|left|caption]]<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:16:16Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - sfBFP (Superfolder Blue Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - sfYFP (Superfolder Yellow Fluorescent Protein)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - sfCFP (Superfolder Cyan Fluorescent Protein)<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|center|594px|border|caption]]<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/File:Superfolderwykres.jpgFile:Superfolderwykres.jpg2013-10-04T23:15:09Z<p>A: uploaded a new version of &quot;File:Superfolderwykres.jpg&quot;</p>
<hr />
<div></div>Ahttp://2013.igem.org/Team:Warsaw/JudgingTeam:Warsaw/Judging2013-10-04T23:13:55Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Judging}}<br />
__NOTOC__<br />
==Requirements for a Bronze Medal==<br />
[[File:Checkbox.png|18px]] Register the team, have a great summer, and plan to have fun at the Regional Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Successfully complete and submit this iGEM 2013 Judging form.<br />
<br />
[[File:Checkbox.png|18px]] Create and share a [https://2013.igem.org/Team:Warsaw/Project_description Description of the team's project] using the iGEM wiki and the [https://2013.igem.org/Team:Warsaw/Parts team's parts using the Registry of Standard Biological Parts].<br />
<br />
[[File:Checkbox.png|18px]] Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br />
[[File:Checkbox.png|18px]] Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. Please note you must submit this new part to the iGEM Registry.<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Superfolderwykres.jpg|center|594px|border|caption]]<br />
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
==Additional Requirements for a Silver Medal==<br />
[[File:Checkbox.png|18px]] Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br />
We measure our superfolder proteins and they fluorescent that we expected.<br />
<br />
[[File:Swieciwszystkoyea.jpg|center|600px|border|caption]]<br />
<br />
[[File:Checkbox.png|18px]] Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br />
Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000]<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001]<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002]<br />
<br />
Other parts that we characterized [https://2013.igem.org/Team:Warsaw/Parts here]<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfCFP<br />
|17,95<br />
|18,38<br />
|17,36<br />
|17,90<br />
|0,51<br />
|-<br />
|sfBFP<br />
|40,65<br />
|41,52<br />
|42,25<br />
|41,47<br />
|0,80<br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|}<br />
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Safety Safety]<br />
<br />
==Additional Requirements for a Gold Medal (one OR more)==<br />
<br />
'''We believe this year we have successfully fulfilled all of the three Gold Medal criteria described below.'''<br />
<br />
[[File:Checkbox.png|18px]] Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br />
The Registry improves not only when users submit great new parts but also when they improve existing parts. For more information see: 2013.igem.org/Judging/Awards<br />
<br />
Part Number(s): <br />
* [http://parts.igem.org/Part:BBa_K1093000 K1093000] - blue fluorescence, improvment of BFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093001 K1093001] - yellow fluorescence, improvment of YFP (superfolder form)<br />
* [http://parts.igem.org/Part:BBa_K1093002 K1093002] - cyan fluorescence, improvment od CFP (superfolder form)<br />
<br />
Superfolder forms of fluorescent proteins are known that are more stable and have stronger fluorescence than non-superfolder forms.<br />
<br />
* [http://parts.igem.org/Part:BBa_K864100 K1093002] - we measure this BioBrick (designed by Team Uppsala 2012) and compare it to our sfYFP.<br />
<br />
[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]<br />
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
[[File:Checkbox.png|18px]] Description of function<br />
<br />
[[File:Checkbox.png|18px]] Quantitative data showing the Part or Device function<br />
<br />
{| class="wikitable" <br />
|-<br />
! scope="col"| -<br />
! colspan="3" | Samples<br />
! scope="col" | Arithmetic mean<br />
! scope="col" | Standard deviation<br />
|-<br />
|sfGFP<br />
|100,60 <br />
|100,37 <br />
|99,02 <br />
|100,00 <br />
|0,85 <br />
|-<br />
|sfYFP<br />
|218,87 <br />
|216,51 <br />
|217,87 <br />
|217,75 <br />
|1,19 <br />
|-<br />
|SYFP2<br />
|424,70 <br />
|418,26 <br />
|412,40 <br />
|418,45 <br />
|6,15 <br />
|-<br />
|}<br />
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''<br />
<br />
<br />
[[File:Checkbox.png|18px]] Acknowledgment of sources and references<br />
<br />
[[File:Checkbox.png|18px]] Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.<br />
<br />
Link to page on your team's wiki: [https://2013.igem.org/Team:Warsaw/Cooperation Cooperation with other iGEM teams]<br />
<br />
[[File:Checkbox.png|18px]] Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br />
==iGEM Prizes==<br />
[[File:Checkbox.png|18px]] Best Human Practice Advance<br />
<br />
Please explain briefly why you should receive any of these special prizes:<br />
* We created first polish manual for Synthetic Biology ("Geny i Maszyny" - eng. "Genes and Machines") with cooperation with polish publishing house - PWN. <br />
* We took part polish Night of Biologists, Science Picnic and Festival of Science. We popularized iGEM and Synthetic Biology [https://2013.igem.org/Team:Warsaw/Strategy_overview See more]<br />
* We cooperated with mass media. See more at: [https://2013.igem.org/Team:Warsaw/Our_cooperation_with_mass_media Cooperation with mass media]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acrylamide_detectionTeam:Warsaw/Acrylamide detection2013-10-04T23:04:59Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Acrylamide detection}}<br />
__TOC__<br />
==Hemoglobin-based sensor==<br />
<br />
The main indicator of exposure to acrylamide are adducts formed at the N-terminal valine of both hemoglobin chains. These can (and have been) detected by coating a glass electrode with human hemoglobin an measuring changes in intensity to voltage ratio (Krajewska, A. ''et al.''). We wish to create a cheaper and simpler variant of this detection system by applying E. coli strain that produces functional human hemoglobin (a modification of BactoBlood system created by Berkley 2007) with both α and β chains fused with split fluorophore fragments derived from sfGFP (superfolder GFP) protein (Zhou, J. ''et al.'') at the C-terminus. Constructs expressing each fusion protein would be transformed into two separate E. coli cultures, which would then be exposed to a solution containing acrylamide and lysed by inducing one of two kill-switches designed this year (see: Safety). <br />
<br />
As the presence of acrylamide adducts at the N-terminus of hemoglobin chains results in a decrease of mutual affinity, it is also expected to lead to a significantly weaker fluorescence in relation to controls. There will be two controls negative: <br />
* detection system in a solution without acrylamide<br />
* bJun/bFos proteins (see: BiFC toolbox) fused with the same split fluorophore fragments and exposed to the same acrylamide solution. Interaction between those proteins should not be affected by acrylamide<br />
<br />
Subunits will be expressed separately, as the formation of functional fluorophore is generally irreversible. sfGFP was chosen for this system, as superfolder mutations result in better resistance to unspecific quenching:<br />
<br />
[[File:Hemo.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
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<br />
==roGFP-based sensor==<br />
In parralel we planned to develop a separate method of detection, based on a redox state reporter - roGFP protein. A fusion of roGFP with human glutaredoxin is a highly specifc reporter of redox potential of cellular glutathione pool (Gutscher, M. ''et al.''). Considering the fact that conjugation with glutathione (by glutathione S-tranferase) is a key pathway of detoxication following acrylamide exposure, its effect on the redox state of gluthatione pool should be easily observable (Cui, S. ''et al.''). <br />
roGFP is a derivative of GFP with two additional cysteines introduced via site-directed mutagenesis. Depending on the redox state, they can form a disulfide bond. This leads to a shift in excitation peak. This effect is more reliable than mere decrease in fluorescence and requires only one simple negative control. As the measurements will be conducted in ''E. coli'' cells, we designed two fusion proteins roGFP with human glutaredoxin and with ''E. coli'' glutaredoxin. Unfortunately, due to delays in gene synthesis, we weren't able to test either of the detection systems in time before wiki freeze.<br />
<br />
[[File:Igemtw structure.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Igemtw graphscopy.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
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<u>Bibliography:</u><br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acrylamide_detectionTeam:Warsaw/Acrylamide detection2013-10-04T23:04:34Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Acrylamide detection}}<br />
__TOC__<br />
==Hemoglobin-based sensor==<br />
<br />
The main indicator of exposure to acrylamide are adducts formed at the N-terminal valine of both hemoglobin chains. These can (and have been) detected by coating a glass electrode with human hemoglobin an measuring changes in intensity to voltage ratio (Krajewska, A. ''et al.''). We wish to create a cheaper and simpler variant of this detection system by applying E. coli strain that produces functional human hemoglobin (a modification of BactoBlood system created by Berkley 2007) with both α and β chains fused with split fluorophore fragments derived from sfGFP (superfolder GFP) protein (Zhou, J. ''et al.'') at the C-terminus. Constructs expressing each fusion protein would be transformed into two separate E. coli cultures, which would then be exposed to a solution containing acrylamide and lysed by inducing one of two kill-switches designed this year (see: Safety). <br />
<br />
As the presence of acrylamide adducts at the N-terminus of hemoglobin chains results in a decrease of mutual affinity, it is also expected to lead to a significantly weaker fluorescence in relation to controls. There will be two controls negative: <br />
* detection system in a solution without acrylamide<br />
* bJun/bFos proteins (see: BiFC toolbox) fused with the same split fluorophore fragments and exposed to the same acrylamide solution. Interaction between those proteins should not be affected by acrylamide<br />
<br />
Subunits will be expressed separately, as the formation of functional fluorophore is generally irreversible. sfGFP was chosen for this system, as superfolder mutations result in better resistance to unspecific quenching:<br />
<br />
[[File:Hemo.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
<br />
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<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
==roGFP-based sensor==<br />
In parralel we planned to develop a separate method of detection, based on a redox state reporter - roGFP protein. A fusion of roGFP with human glutaredoxin is a highly specifc reporter of redox potential of cellular glutathione pool (Gutscher, M. ''et al.''). Considering the fact that conjugation with glutathione (by glutathione S-tranferase) is a key pathway of detoxication following acrylamide exposure, its effect on the redox state of gluthatione pool should be easily observable (Cui, S. ''et al.''). <br />
roGFP is a derivative of GFP with two additional cysteines introduced via site-directed mutagenesis. Depending on the redox state, they can form a disulfide bond. This leads to a shift in excitation peak. This effect is more reliable than mere decrease in fluorescence and requires only one simple negative control. As the measurements will be conducted in ''E. coli'' cells, we designed two fusion proteins roGFP with human glutaredoxin and with ''E. coli'' glutaredoxin. Unfortunately, due to delays in gene synthesis, we weren't able to test either of the detection systems in time before wiki freeze.<br />
<br />
[[File:Igemtw structure.png|600px|thumb|eft|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File:Igemtw graphscopy.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
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<br />
<u>Bibliography:</u><br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acrylamide_detectionTeam:Warsaw/Acrylamide detection2013-10-04T23:03:57Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Acrylamide detection}}<br />
__TOC__<br />
==Hemoglobin-based sensor==<br />
<br />
The main indicator of exposure to acrylamide are adducts formed at the N-terminal valine of both hemoglobin chains. These can (and have been) detected by coating a glass electrode with human hemoglobin an measuring changes in intensity to voltage ratio (Krajewska, A. ''et al.''). We wish to create a cheaper and simpler variant of this detection system by applying E. coli strain that produces functional human hemoglobin (a modification of BactoBlood system created by Berkley 2007) with both α and β chains fused with split fluorophore fragments derived from sfGFP (superfolder GFP) protein (Zhou, J. ''et al.'') at the C-terminus. Constructs expressing each fusion protein would be transformed into two separate E. coli cultures, which would then be exposed to a solution containing acrylamide and lysed by inducing one of two kill-switches designed this year (see: Safety). <br />
<br />
As the presence of acrylamide adducts at the N-terminus of hemoglobin chains results in a decrease of mutual affinity, it is also expected to lead to a significantly weaker fluorescence in relation to controls. There will be two controls negative: <br />
* detection system in a solution without acrylamide<br />
* bJun/bFos proteins (see: BiFC toolbox) fused with the same split fluorophore fragments and exposed to the same acrylamide solution. Interaction between those proteins should not be affected by acrylamide<br />
<br />
Subunits will be expressed separately, as the formation of functional fluorophore is generally irreversible. sfGFP was chosen for this system, as superfolder mutations result in better resistance to unspecific quenching:<br />
<br />
[[File:Hemo.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
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<br />
<br />
<br />
<br />
<br />
<br />
<br />
==roGFP-based sensor==<br />
In parralel we planned to develop a separate method of detection, based on a redox state reporter - roGFP protein. A fusion of roGFP with human glutaredoxin is a highly specifc reporter of redox potential of cellular glutathione pool (Gutscher, M. ''et al.''). Considering the fact that conjugation with glutathione (by glutathione S-tranferase) is a key pathway of detoxication following acrylamide exposure, its effect on the redox state of gluthatione pool should be easily observable (Cui, S. ''et al.''). <br />
roGFP is a derivative of GFP with two additional cysteines introduced via site-directed mutagenesis. Depending on the redox state, they can form a disulfide bond. This leads to a shift in excitation peak. This effect is more reliable than mere decrease in fluorescence and requires only one simple negative control. As the measurements will be conducted in ''E. coli'' cells, we designed two fusion proteins roGFP with human glutaredoxin and with ''E. coli'' glutaredoxin. Unfortunately, due to delays in gene synthesis, we weren't able to test either of the detection systems in time before wiki freeze.<br />
<br />
[[File:Igemtw structure.png|600px|thumb|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
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[[File:Igemtw graphscopy.png|600px|thumb|A simplified overview of the hemoglobin-based sensor.]]<br />
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<u>Bibliography:</u><br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acrylamide_detectionTeam:Warsaw/Acrylamide detection2013-10-04T23:03:10Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Acrylamide detection}}<br />
__TOC__<br />
==Hemoglobin-based sensor==<br />
<br />
The main indicator of exposure to acrylamide are adducts formed at the N-terminal valine of both hemoglobin chains. These can (and have been) detected by coating a glass electrode with human hemoglobin an measuring changes in intensity to voltage ratio (Krajewska, A. ''et al.''). We wish to create a cheaper and simpler variant of this detection system by applying E. coli strain that produces functional human hemoglobin (a modification of BactoBlood system created by Berkley 2007) with both α and β chains fused with split fluorophore fragments derived from sfGFP (superfolder GFP) protein (Zhou, J. ''et al.'') at the C-terminus. Constructs expressing each fusion protein would be transformed into two separate E. coli cultures, which would then be exposed to a solution containing acrylamide and lysed by inducing one of two kill-switches designed this year (see: Safety). <br />
<br />
As the presence of acrylamide adducts at the N-terminus of hemoglobin chains results in a decrease of mutual affinity, it is also expected to lead to a significantly weaker fluorescence in relation to controls. There will be two controls negative: <br />
* detection system in a solution without acrylamide<br />
* bJun/bFos proteins (see: BiFC toolbox) fused with the same split fluorophore fragments and exposed to the same acrylamide solution. Interaction between those proteins should not be affected by acrylamide<br />
<br />
Subunits will be expressed separately, as the formation of functional fluorophore is generally irreversible. sfGFP was chosen for this system, as superfolder mutations result in better resistance to unspecific quenching:<br />
<br />
[[File:Hemo.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
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==roGFP-based sensor==<br />
In parralel we planned to develop a separate method of detection, based on a redox state reporter - roGFP protein. A fusion of roGFP with human glutaredoxin is a highly specifc reporter of redox potential of cellular glutathione pool (Gutscher, M. ''et al.''). Considering the fact that conjugation with glutathione (by glutathione S-tranferase) is a key pathway of detoxication following acrylamide exposure, its effect on the redox state of gluthatione pool should be easily observable (Cui, S. ''et al.''). <br />
roGFP is a derivative of GFP with two additional cysteines introduced via site-directed mutagenesis. Depending on the redox state, they can form a disulfide bond. This leads to a shift in excitation peak. This effect is more reliable than mere decrease in fluorescence and requires only one simple negative control. As the measurements will be conducted in ''E. coli'' cells, we designed two fusion proteins roGFP with human glutaredoxin and with ''E. coli'' glutaredoxin. Unfortunately, due to delays in gene synthesis, we weren't able to test either of the detection systems in time before wiki freeze.<br />
<br />
{| class="wikitable"<br />
|- border="0"<br />
| [[File:Igemtw structure.png|600px]]<br />
| Tertiary structure of roGFP with two possible redox states of the cysteines introduced by site-directed mutagenesis <br />
|-<br />
|}<br />
<br />
[[File:Igemtw structure.png|600px|thumb|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
[[File:Igemtw graphscopy.png|600px|thumb|A simplified overview of the hemoglobin-based sensor.]]<br />
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<br />
<u>Bibliography:</u><br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/Acrylamide_detectionTeam:Warsaw/Acrylamide detection2013-10-04T23:02:16Z<p>A: /* roGFP-based sensor */</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Acrylamide detection}}<br />
__TOC__<br />
==Hemoglobin-based sensor==<br />
<br />
The main indicator of exposure to acrylamide are adducts formed at the N-terminal valine of both hemoglobin chains. These can (and have been) detected by coating a glass electrode with human hemoglobin an measuring changes in intensity to voltage ratio (Krajewska, A. ''et al.''). We wish to create a cheaper and simpler variant of this detection system by applying E. coli strain that produces functional human hemoglobin (a modification of BactoBlood system created by Berkley 2007) with both α and β chains fused with split fluorophore fragments derived from sfGFP (superfolder GFP) protein (Zhou, J. ''et al.'') at the C-terminus. Constructs expressing each fusion protein would be transformed into two separate E. coli cultures, which would then be exposed to a solution containing acrylamide and lysed by inducing one of two kill-switches designed this year (see: Safety). <br />
<br />
As the presence of acrylamide adducts at the N-terminus of hemoglobin chains results in a decrease of mutual affinity, it is also expected to lead to a significantly weaker fluorescence in relation to controls. There will be two controls negative: <br />
* detection system in a solution without acrylamide<br />
* bJun/bFos proteins (see: BiFC toolbox) fused with the same split fluorophore fragments and exposed to the same acrylamide solution. Interaction between those proteins should not be affected by acrylamide<br />
<br />
Subunits will be expressed separately, as the formation of functional fluorophore is generally irreversible. sfGFP was chosen for this system, as superfolder mutations result in better resistance to unspecific quenching:<br />
<br />
[[File:Hemo.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
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<br />
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<br />
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<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
==roGFP-based sensor==<br />
In parralel we planned to develop a separate method of detection, based on a redox state reporter - roGFP protein. A fusion of roGFP with human glutaredoxin is a highly specifc reporter of redox potential of cellular glutathione pool (Gutscher, M. ''et al.''). Considering the fact that conjugation with glutathione (by glutathione S-tranferase) is a key pathway of detoxication following acrylamide exposure, its effect on the redox state of gluthatione pool should be easily observable (Cui, S. ''et al.''). <br />
roGFP is a derivative of GFP with two additional cysteines introduced via site-directed mutagenesis. Depending on the redox state, they can form a disulfide bond. This leads to a shift in excitation peak. This effect is more reliable than mere decrease in fluorescence and requires only one simple negative control. As the measurements will be conducted in ''E. coli'' cells, we designed two fusion proteins roGFP with human glutaredoxin and with ''E. coli'' glutaredoxin. Unfortunately, due to delays in gene synthesis, we weren't able to test either of the detection systems in time before wiki freeze.<br />
<br />
{| class="wikitable"<br />
|- border="0"<br />
| [[File:Igemtw structure.png|600px]]<br />
| Tertiary structure of roGFP with two possible redox states of the cysteines introduced by site-directed mutagenesis <br />
|-<br />
|}<br />
<br />
[[File:Igemtw graphscopy.png|600px|thumb|left|A simplified overview of the hemoglobin-based sensor.]]<br />
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<br />
<u>Bibliography:</u><br />
# Krajewska, A., Radecki, J. & Radecka, H. A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors 8, 5832–5844 (2008).<br />
# Berkeley_UC @ 2007.igem.org. at [https://2007.igem.org/Berkeley_UC]<br />
# Zhou, J., Lin, J. & Zhou, C. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta biochimica et … 43, 239–244 (2011).<br />
# Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C. & Waldo, G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology 24, 79–88 (2006).<br />
# Hanson, G. T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. The Journal of biological chemistry 279, 13044–53 (2004).<br />
# Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nature methods 5, 553–9 (2008).<br />
# Cui, S., Kim, S., Jo, S. & Lee, Y. A study of in vitro scavenging reactions of acrylamide with glutathione using electrospray ionization tandem mass spectrometry. BULLETIN-KOREAN … (2005).at [http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B050816]<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/CytotoxicityTeam:Warsaw/Cytotoxicity2013-10-04T22:59:17Z<p>A: Undo revision 306989 by A (talk)</p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Cytotoxicity study}}<br />
__TOC__<br />
==Overview==<br />
The neurotoxic effects of acrylamide have been widely known for many years. Local symptoms of acrylamide poisoning such as mucous membrane and skin irritation have been formerly reported. There also exists a systemic effect characterized by fatigue, sleepiness and memory difficulties; in extreme cases also hallucination, disorientation and confusion were observed. <br />
<br />
While acrylamide was perceived as a severe neurotoxin, there had long not been enough studies on its possible carcinogenicity. Yet, the recent research have shown that acrylamide may be engaged in processes. <br />
Due to the rapid development of the plastic industry, acrylamide is becoming even more widely present in our environment. But the manufacturing area is not the only way by which acrylamide appears in our proximity. While deep-frying or baking, through a chemical reaction between an amino acid and a reducing sugar (called the Maillard reaction), acrylamide is formed. <br />
<br />
Possible ways of acrylamide absorption are as follows: inhalation, ingestion, and through the skin and mucous membranes. Basing on a report of WHO from 2002 average daily intake for the general population ranges from 0.3 to 0.8 micrograms of acrylamide per kilogram of body mass. <br />
<br />
Increased consumption of deep-fried food and, subsequently, increased intake of acrylamide, may have serious health consequences. Recent studies have revealed an association between consumption of deep-fried foods and increased prostate cancer risk. What is also suggested, is that there is positive correlation between acrylamide intake and increased risk of endometrial and ovarian cancer. <br />
<br />
Considering the alarming effects of acrylamide exposure, we have decided to include in our project a cytotoxicity study. Our aim is to show how acrylamide may affect various cell lines originating from different tissues. In our experiments we are working on HeLa, 293 and 143B cell lines representing cervical cancer, human embryonic kidney and bone osteosarcoma cells respectively. <br />
<br />
In our experiments we are implementing the following methods: Flow Cytometry, AlamarBlue assay, Cell Growth Assay, Cell Cycle assay and Caspase activity assay.<br />
<br />
To indicate cell viability, we have decided to implement the Alamar Blue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule - resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally, which can be use to measure viability of the cells.<br />
<br />
Flow cytometry enables us to conduct more precise analysis of chosen aspects of cell biology. After conducting flow cytometry, we will be able to state whether treated cells are undergoing apoptosis, necrosis or if the observed metabolic effects are only cytostatic. <br />
<br />
In order to state how acrylamide may influence the progression of cell cycle we have decided to implement Cell Cycle Assay. This method employs flow cytometry to mesure number of cells in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measure the fluorescence. PI binds to DNA quantitatively so the fluorescence intensity of the stained cells is correlated with the amount of their DNA. Thus, basing on the quantity of DNA we are enabled to assign cells to different cell cycle phases. <br />
<br />
We expect to observe the cytotoxic effects in higher acrylamide concentrations i.e. 3-5 mM. In lower concentrations <1 mM may occur the elimination of the toxicant, whose effects are to be examined. <br />
<br />
<u>Bibliography:</u><br />
# Stott-Miller M, Neuhouser ML, Stanford JL; ''Consumption of deep-fried foods and risk of prostate cancer''; Epub 2013 Jan 17.<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A; ''Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes''; Epub 2012 Dec 31<br />
# Ahmad Besaratinia_ and Gerd P.Pfeifer; ''A review of mechanisms of acrylamide carcinogenicity''; Carcinogenesis vol.28 no.3 pp.519–528, 2007<br />
# O'Brien J, Wilson I, Orton T, Pognan F; ''Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity''; Eur J Biochem. 2000 Sep;267(17):5421-6.<br />
# Naoki Koyamaa,b,c, Hiroko Sakamoto a, Mayumi Sakuraba a, Tomoko Koizumi a, Yoshio Takashima a, Makoto Hayashi a, Hiroshi Matsufuji b, Kazuo Yamagata b, Shuichi Masudac, Naohide Kinae c, Masamitsu Honmaa,; ''Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells''; Mutation Research 603 (2006) 151–158<br />
<br />
==Results==<br />
<br />
After conducting our experiments we have obtained following results:<br />
* loss of metabolic activity (results obtained from AlamarBlue assay)<br />
* induction of apoptotic cell death (stated by the presence of annexinV positive cells and the observation of caspase activity)<br />
* suppression of cell growth and proliferation (stated on the basis of Cell Growth assay and AlamarBlue assay)<br />
* disturbances in the cell cycle (based on the outcomes of Cell Cycle assay)<br />
<br />
==Getting the experiments underway==<br />
<br />
As a starting point for our experiments we have decided to implement AlamarBlue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule- resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally and can be use to measure viability of the cells. AlamarBlue assay provides reliable and repeatable results yet is easy to conduct thus it serves as a trustworthy basis for further research. <br />
<br />
In order to obtain reliable results of experiments we have decided to conduct preliminary assays to determine plating density and concentration of acrylamide for each cell line: HeLa, 293 and 143B. The protocol of the assay as well as detailed conduct can be found in the section: Extras.<br />
<br />
The outcome of AlamarBlue assay showed the existence of strong, negative correlation between acrylamide exposure and cell viability (Figure 1.). So the longer exposure time and the higher concentration of acrylamide, the weaker the cell viability. <br />
<br />
[[File:avHEK2.png|550px|frameless|border|alt=A|Figure 1. Influence of acrylamide on 293 cells viability. Cells were treated with various concentration of acrylamide and the viability was measured after indicated period of time.|]]<br />
<br />
In order to state whether the loss of cell viability, that we have observed, was caused by decrease in cell division (the cytostatic effect of acrylamide) or by cell death (the cytotoxic effect of acrylamide) we have implemented Cell growth assays. To conduct the assays we have seeded cells from different cell lines (HeLa, 293 and 143B) in 6-wells plates adding proper concentration of acrylamide to each well and counted the amount of cells in counting chamber after trypan blue staining, for 24, 48 and 72 hours incubation. This approach indicated that acrylamide treatment affects cell proliferation and induces cell death.<br />
<br />
[[File:CG2HeLa.png|frameless|border|caption|850px]]<br />
<br />
[[File:CGA-HeLa.png|frameless|border|caption|550px]]<br />
<br />
In order to bring under scrutiny changes in the cell cycle we've carried out Cell Cycle assay. We observed that there occur disturbances in the cell cycle depending on concentration of acrylamide and incubation time.<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Histograms for Cell Cycle assay"><br />
<br />
<br />
File:HeLa-hist.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
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File:143B-hist.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
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File:HEK293-hist.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
<br />
</gallery><br />
<br />
<html><br />
These results made us to consider further investigation. As we wanted to state whether acrylamide induces apoptotic cell death we decided to measure caspase activity and examine presence of the cells that would bind annexinV (which are hallmarks of apoptosis). <br />
<a href="http://pl.promega.com/products/cell-health-and-metabolism/apoptosis-assays/luminescent-caspase-assays/caspase_glo-3_7-assay-systems/?origUrl=http%3a%2f%2fwww.promega.com%2fproducts%2fcell-health-and-metabolism%2fapoptosis-assays%2fluminescent-caspase-assays%2fcaspase_glo-3_7-assay-systems%2f" target="_blank">Caspase-Glo 3/7 Assay</a> revealed that there is an increase in caspase activity caused by acrylamide treatement. <br />
The Flow Cytometry analysis showed that there is a growth in annexinV positive cells number due to addition of acrylamide. <br />
<br />
<br />
</html><br />
<br />
[[File:caspase1.png|frameless|border|caption|450px]]<br />
<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Figures for the Flow Cytometry"><br />
<br />
<br />
File:HeLa-FC.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-FC.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
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File:HEK-FC.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
</gallery><br />
<br />
In our study we've included Cisplatin control, whose results are to be seen in section: [https://2013.igem.org/Team:Warsaw/Notebook Notebook]<br />
<br />
All of the results as well as graphs, figures and statistics are to be seen in section: [https://2013.igem.org/Team:Warsaw/Cellular_biology_lab_journal Cellular biology lab journal]<br />
<br />
==Summary==<br />
<br />
Despite the fact that it is impossible to relate directly the results obtained for the cell lines to human organism, it may give a basis for further examination and extended research. In our experiments '''we showed the existence of cytotoxic and cytostatic effect of acrylamide on HeLa, 143B and 293 cell lines. We also found a correlation between time of exposure to acrylamide and the decrease in cell viability and proliferation.''' <br />
<br />
By carrying out our cytotoxicity study '''we wanted to emphasize the importance of synthetic biology research'''. After showing the possible harmful effects of acrylamide we aimed to reveal how products of synthetic biology may influence and facilitate our everyday life. We also tried to raise the awareness of the threats connected with the consumption of tertiary processed foods. <br />
<br />
Finally, let us make you imagine that you are able to check the amount of acrylamide before eating your chips. With our FluoSafe sensor it will be soon possible.<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/CytotoxicityTeam:Warsaw/Cytotoxicity2013-10-04T22:58:26Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Cytotoxicity study}}<br />
__TOC__<br />
==Overview==<br />
The neurotoxic effects of acrylamide have been widely known for many years. Local symptoms of acrylamide poisoning such as mucous membrane and skin irritation have been formerly reported. There also exists a systemic effect characterized by fatigue, sleepiness and memory difficulties; in extreme cases also hallucination, disorientation and confusion were observed. <br />
<br />
While acrylamide was perceived as a severe neurotoxin, there had long not been enough studies on its possible carcinogenicity. Yet, the recent research have shown that acrylamide may be engaged in processes. <br />
Due to the rapid development of the plastic industry, acrylamide is becoming even more widely present in our environment. But the manufacturing area is not the only way by which acrylamide appears in our proximity. While deep-frying or baking, through a chemical reaction between an amino acid and a reducing sugar (called the Maillard reaction), acrylamide is formed. <br />
<br />
Possible ways of acrylamide absorption are as follows: inhalation, ingestion, and through the skin and mucous membranes. Basing on a report of WHO from 2002 average daily intake for the general population ranges from 0.3 to 0.8 micrograms of acrylamide per kilogram of body mass. <br />
<br />
Increased consumption of deep-fried food and, subsequently, increased intake of acrylamide, may have serious health consequences. Recent studies have revealed an association between consumption of deep-fried foods and increased prostate cancer risk. What is also suggested, is that there is positive correlation between acrylamide intake and increased risk of endometrial and ovarian cancer. <br />
<br />
Considering the alarming effects of acrylamide exposure, we have decided to include in our project a cytotoxicity study. Our aim is to show how acrylamide may affect various cell lines originating from different tissues. In our experiments we are working on HeLa, 293 and 143B cell lines representing cervical cancer, human embryonic kidney and bone osteosarcoma cells respectively. <br />
<br />
In our experiments we are implementing the following methods: Flow Cytometry, AlamarBlue assay, Cell Growth Assay, Cell Cycle assay and Caspase activity assay.<br />
<br />
To indicate cell viability, we have decided to implement the Alamar Blue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule - resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally, which can be use to measure viability of the cells.<br />
<br />
Flow cytometry enables us to conduct more precise analysis of chosen aspects of cell biology. After conducting flow cytometry, we will be able to state whether treated cells are undergoing apoptosis, necrosis or if the observed metabolic effects are only cytostatic. <br />
<br />
In order to state how acrylamide may influence the progression of cell cycle we have decided to implement Cell Cycle Assay. This method employs flow cytometry to mesure number of cells in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measure the fluorescence. PI binds to DNA quantitatively so the fluorescence intensity of the stained cells is correlated with the amount of their DNA. Thus, basing on the quantity of DNA we are enabled to assign cells to different cell cycle phases. <br />
<br />
We expect to observe the cytotoxic effects in higher acrylamide concentrations i.e. 3-5 mM. In lower concentrations <1 mM may occur the elimination of the toxicant, whose effects are to be examined. <br />
<br />
<u>Bibliography:</u><br />
# Stott-Miller M, Neuhouser ML, Stanford JL; ''Consumption of deep-fried foods and risk of prostate cancer''; Epub 2013 Jan 17.<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A; ''Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes''; Epub 2012 Dec 31<br />
# Ahmad Besaratinia_ and Gerd P.Pfeifer; ''A review of mechanisms of acrylamide carcinogenicity''; Carcinogenesis vol.28 no.3 pp.519–528, 2007<br />
# O'Brien J, Wilson I, Orton T, Pognan F; ''Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity''; Eur J Biochem. 2000 Sep;267(17):5421-6.<br />
# Naoki Koyamaa,b,c, Hiroko Sakamoto a, Mayumi Sakuraba a, Tomoko Koizumi a, Yoshio Takashima a, Makoto Hayashi a, Hiroshi Matsufuji b, Kazuo Yamagata b, Shuichi Masudac, Naohide Kinae c, Masamitsu Honmaa,; ''Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells''; Mutation Research 603 (2006) 151–158<br />
<br />
==Results==<br />
<br />
After conducting our experiments we have obtained following results:<br />
* loss of metabolic activity (results obtained from AlamarBlue assay)<br />
* induction of apoptotic cell death (stated by the presence of annexinV positive cells and the observation of caspase activity)<br />
* suppression of cell growth and proliferation (stated on the basis of Cell Growth assay and AlamarBlue assay)<br />
* disturbances in the cell cycle (based on the outcomes of Cell Cycle assay)<br />
<br />
==Getting the experiments underway==<br />
<br />
As a starting point for our experiments we have decided to implement AlamarBlue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule- resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally and can be use to measure viability of the cells. AlamarBlue assay provides reliable and repeatable results yet is easy to conduct thus it serves as a trustworthy basis for further research. <br />
<br />
In order to obtain reliable results of experiments we have decided to conduct preliminary assays to determine plating density and concentration of acrylamide for each cell line: HeLa, 293 and 143B. The protocol of the assay as well as detailed conduct can be found in the section: Extras.<br />
<br />
The outcome of AlamarBlue assay showed the existence of strong, negative correlation between acrylamide exposure and cell viability (Figure 1.). So the longer exposure time and the higher concentration of acrylamide, the weaker the cell viability. <br />
<br />
[[File:avHEK2.png|550px|Figure 1. Influence of acrylamide on 293 cells viability. Cells were treated with various concentration of acrylamide and the viability was measured after indicated period of time.|]]<br />
<br />
In order to state whether the loss of cell viability, that we have observed, was caused by decrease in cell division (the cytostatic effect of acrylamide) or by cell death (the cytotoxic effect of acrylamide) we have implemented Cell growth assays. To conduct the assays we have seeded cells from different cell lines (HeLa, 293 and 143B) in 6-wells plates adding proper concentration of acrylamide to each well and counted the amount of cells in counting chamber after trypan blue staining, for 24, 48 and 72 hours incubation. This approach indicated that acrylamide treatment affects cell proliferation and induces cell death.<br />
<br />
[[File:CG2HeLa.png|frameless|border|caption|850px]]<br />
<br />
[[File:CGA-HeLa.png|frameless|border|caption|550px]]<br />
<br />
In order to bring under scrutiny changes in the cell cycle we've carried out Cell Cycle assay. We observed that there occur disturbances in the cell cycle depending on concentration of acrylamide and incubation time.<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Histograms for Cell Cycle assay"><br />
<br />
<br />
File:HeLa-hist.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-hist.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK293-hist.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
<br />
</gallery><br />
<br />
<html><br />
These results made us to consider further investigation. As we wanted to state whether acrylamide induces apoptotic cell death we decided to measure caspase activity and examine presence of the cells that would bind annexinV (which are hallmarks of apoptosis). <br />
<a href="http://pl.promega.com/products/cell-health-and-metabolism/apoptosis-assays/luminescent-caspase-assays/caspase_glo-3_7-assay-systems/?origUrl=http%3a%2f%2fwww.promega.com%2fproducts%2fcell-health-and-metabolism%2fapoptosis-assays%2fluminescent-caspase-assays%2fcaspase_glo-3_7-assay-systems%2f" target="_blank">Caspase-Glo 3/7 Assay</a> revealed that there is an increase in caspase activity caused by acrylamide treatement. <br />
The Flow Cytometry analysis showed that there is a growth in annexinV positive cells number due to addition of acrylamide. <br />
<br />
<br />
</html><br />
<br />
[[File:caspase1.png|frameless|border|caption|450px]]<br />
<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Figures for the Flow Cytometry"><br />
<br />
<br />
File:HeLa-FC.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-FC.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK-FC.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
</gallery><br />
<br />
In our study we've included Cisplatin control, whose results are to be seen in section: [https://2013.igem.org/Team:Warsaw/Notebook Notebook]<br />
<br />
All of the results as well as graphs, figures and statistics are to be seen in section: [https://2013.igem.org/Team:Warsaw/Cellular_biology_lab_journal Cellular biology lab journal]<br />
<br />
==Summary==<br />
<br />
Despite the fact that it is impossible to relate directly the results obtained for the cell lines to human organism, it may give a basis for further examination and extended research. In our experiments '''we showed the existence of cytotoxic and cytostatic effect of acrylamide on HeLa, 143B and 293 cell lines. We also found a correlation between time of exposure to acrylamide and the decrease in cell viability and proliferation.''' <br />
<br />
By carrying out our cytotoxicity study '''we wanted to emphasize the importance of synthetic biology research'''. After showing the possible harmful effects of acrylamide we aimed to reveal how products of synthetic biology may influence and facilitate our everyday life. We also tried to raise the awareness of the threats connected with the consumption of tertiary processed foods. <br />
<br />
Finally, let us make you imagine that you are able to check the amount of acrylamide before eating your chips. With our FluoSafe sensor it will be soon possible.<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/CytotoxicityTeam:Warsaw/Cytotoxicity2013-10-04T22:56:58Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Cytotoxicity study}}<br />
__TOC__<br />
==Overview==<br />
The neurotoxic effects of acrylamide have been widely known for many years. Local symptoms of acrylamide poisoning such as mucous membrane and skin irritation have been formerly reported. There also exists a systemic effect characterized by fatigue, sleepiness and memory difficulties; in extreme cases also hallucination, disorientation and confusion were observed. <br />
<br />
While acrylamide was perceived as a severe neurotoxin, there had long not been enough studies on its possible carcinogenicity. Yet, the recent research have shown that acrylamide may be engaged in processes. <br />
Due to the rapid development of the plastic industry, acrylamide is becoming even more widely present in our environment. But the manufacturing area is not the only way by which acrylamide appears in our proximity. While deep-frying or baking, through a chemical reaction between an amino acid and a reducing sugar (called the Maillard reaction), acrylamide is formed. <br />
<br />
Possible ways of acrylamide absorption are as follows: inhalation, ingestion, and through the skin and mucous membranes. Basing on a report of WHO from 2002 average daily intake for the general population ranges from 0.3 to 0.8 micrograms of acrylamide per kilogram of body mass. <br />
<br />
Increased consumption of deep-fried food and, subsequently, increased intake of acrylamide, may have serious health consequences. Recent studies have revealed an association between consumption of deep-fried foods and increased prostate cancer risk. What is also suggested, is that there is positive correlation between acrylamide intake and increased risk of endometrial and ovarian cancer. <br />
<br />
Considering the alarming effects of acrylamide exposure, we have decided to include in our project a cytotoxicity study. Our aim is to show how acrylamide may affect various cell lines originating from different tissues. In our experiments we are working on HeLa, 293 and 143B cell lines representing cervical cancer, human embryonic kidney and bone osteosarcoma cells respectively. <br />
<br />
In our experiments we are implementing the following methods: Flow Cytometry, AlamarBlue assay, Cell Growth Assay, Cell Cycle assay and Caspase activity assay.<br />
<br />
To indicate cell viability, we have decided to implement the Alamar Blue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule - resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally, which can be use to measure viability of the cells.<br />
<br />
Flow cytometry enables us to conduct more precise analysis of chosen aspects of cell biology. After conducting flow cytometry, we will be able to state whether treated cells are undergoing apoptosis, necrosis or if the observed metabolic effects are only cytostatic. <br />
<br />
In order to state how acrylamide may influence the progression of cell cycle we have decided to implement Cell Cycle Assay. This method employs flow cytometry to mesure number of cells in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measure the fluorescence. PI binds to DNA quantitatively so the fluorescence intensity of the stained cells is correlated with the amount of their DNA. Thus, basing on the quantity of DNA we are enabled to assign cells to different cell cycle phases. <br />
<br />
We expect to observe the cytotoxic effects in higher acrylamide concentrations i.e. 3-5 mM. In lower concentrations <1 mM may occur the elimination of the toxicant, whose effects are to be examined. <br />
<br />
<u>Bibliography:</u><br />
# Stott-Miller M, Neuhouser ML, Stanford JL; ''Consumption of deep-fried foods and risk of prostate cancer''; Epub 2013 Jan 17.<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A; ''Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes''; Epub 2012 Dec 31<br />
# Ahmad Besaratinia_ and Gerd P.Pfeifer; ''A review of mechanisms of acrylamide carcinogenicity''; Carcinogenesis vol.28 no.3 pp.519–528, 2007<br />
# O'Brien J, Wilson I, Orton T, Pognan F; ''Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity''; Eur J Biochem. 2000 Sep;267(17):5421-6.<br />
# Naoki Koyamaa,b,c, Hiroko Sakamoto a, Mayumi Sakuraba a, Tomoko Koizumi a, Yoshio Takashima a, Makoto Hayashi a, Hiroshi Matsufuji b, Kazuo Yamagata b, Shuichi Masudac, Naohide Kinae c, Masamitsu Honmaa,; ''Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells''; Mutation Research 603 (2006) 151–158<br />
<br />
==Results==<br />
<br />
After conducting our experiments we have obtained following results:<br />
* loss of metabolic activity (results obtained from AlamarBlue assay)<br />
* induction of apoptotic cell death (stated by the presence of annexinV positive cells and the observation of caspase activity)<br />
* suppression of cell growth and proliferation (stated on the basis of Cell Growth assay and AlamarBlue assay)<br />
* disturbances in the cell cycle (based on the outcomes of Cell Cycle assay)<br />
<br />
==Getting the experiments underway==<br />
<br />
As a starting point for our experiments we have decided to implement AlamarBlue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule- resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally and can be use to measure viability of the cells. AlamarBlue assay provides reliable and repeatable results yet is easy to conduct thus it serves as a trustworthy basis for further research. <br />
<br />
In order to obtain reliable results of experiments we have decided to conduct preliminary assays to determine plating density and concentration of acrylamide for each cell line: HeLa, 293 and 143B. The protocol of the assay as well as detailed conduct can be found in the section: Extras.<br />
<br />
The outcome of AlamarBlue assay showed the existence of strong, negative correlation between acrylamide exposure and cell viability (Figure 1.). So the longer exposure time and the higher concentration of acrylamide, the weaker the cell viability. <br />
<br />
[[File:avHEK2.png|550px|frameless|border|alt=A|Figure 1. Influence of acrylamide on 293 cells viability. Cells were treated with various concentration of acrylamide and the viability was measured after indicated period of time.|]]<br />
<br />
In order to state whether the loss of cell viability, that we have observed, was caused by decrease in cell division (the cytostatic effect of acrylamide) or by cell death (the cytotoxic effect of acrylamide) we have implemented Cell growth assays. To conduct the assays we have seeded cells from different cell lines (HeLa, 293 and 143B) in 6-wells plates adding proper concentration of acrylamide to each well and counted the amount of cells in counting chamber after trypan blue staining, for 24, 48 and 72 hours incubation. This approach indicated that acrylamide treatment affects cell proliferation and induces cell death.<br />
<br />
[[File:CG2HeLa.png|frameless|border|caption|850px]]<br />
<br />
[[File:CGA-HeLa.png|frameless|border|caption|550px]]<br />
<br />
In order to bring under scrutiny changes in the cell cycle we've carried out Cell Cycle assay. We observed that there occur disturbances in the cell cycle depending on concentration of acrylamide and incubation time.<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Histograms for Cell Cycle assay"><br />
<br />
<br />
File:HeLa-hist.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-hist.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK293-hist.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
<br />
</gallery><br />
<br />
<html><br />
These results made us to consider further investigation. As we wanted to state whether acrylamide induces apoptotic cell death we decided to measure caspase activity and examine presence of the cells that would bind annexinV (which are hallmarks of apoptosis). <br />
<a href="http://pl.promega.com/products/cell-health-and-metabolism/apoptosis-assays/luminescent-caspase-assays/caspase_glo-3_7-assay-systems/?origUrl=http%3a%2f%2fwww.promega.com%2fproducts%2fcell-health-and-metabolism%2fapoptosis-assays%2fluminescent-caspase-assays%2fcaspase_glo-3_7-assay-systems%2f" target="_blank">Caspase-Glo 3/7 Assay</a> revealed that there is an increase in caspase activity caused by acrylamide treatement. <br />
The Flow Cytometry analysis showed that there is a growth in annexinV positive cells number due to addition of acrylamide. <br />
<br />
<br />
</html><br />
<br />
[[File:caspase1.png|frameless|border|caption|450px]]<br />
<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Figures for the Flow Cytometry"><br />
<br />
<br />
File:HeLa-FC.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-FC.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK-FC.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
</gallery><br />
<br />
In our study we've included Cisplatin control, whose results are to be seen in section: [https://2013.igem.org/Team:Warsaw/Notebook Notebook]<br />
<br />
All of the results as well as graphs, figures and statistics are to be seen in section: [https://2013.igem.org/Team:Warsaw/Cellular_biology_lab_journal Cellular biology lab journal]<br />
<br />
==Summary==<br />
<br />
Despite the fact that it is impossible to relate directly the results obtained for the cell lines to human organism, it may give a basis for further examination and extended research. In our experiments '''we showed the existence of cytotoxic and cytostatic effect of acrylamide on HeLa, 143B and 293 cell lines. We also found a correlation between time of exposure to acrylamide and the decrease in cell viability and proliferation.''' <br />
<br />
By carrying out our cytotoxicity study '''we wanted to emphasize the importance of synthetic biology research'''. After showing the possible harmful effects of acrylamide we aimed to reveal how products of synthetic biology may influence and facilitate our everyday life. We also tried to raise the awareness of the threats connected with the consumption of tertiary processed foods. <br />
<br />
Finally, let us make you imagine that you are able to check the amount of acrylamide before eating your chips. With our FluoSafe sensor it will be soon possible.<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/Team:Warsaw/CytotoxicityTeam:Warsaw/Cytotoxicity2013-10-04T22:56:28Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Cytotoxicity study}}<br />
__TOC__<br />
==Overview==<br />
The neurotoxic effects of acrylamide have been widely known for many years. Local symptoms of acrylamide poisoning such as mucous membrane and skin irritation have been formerly reported. There also exists a systemic effect characterized by fatigue, sleepiness and memory difficulties; in extreme cases also hallucination, disorientation and confusion were observed. <br />
<br />
While acrylamide was perceived as a severe neurotoxin, there had long not been enough studies on its possible carcinogenicity. Yet, the recent research have shown that acrylamide may be engaged in processes. <br />
Due to the rapid development of the plastic industry, acrylamide is becoming even more widely present in our environment. But the manufacturing area is not the only way by which acrylamide appears in our proximity. While deep-frying or baking, through a chemical reaction between an amino acid and a reducing sugar (called the Maillard reaction), acrylamide is formed. <br />
<br />
Possible ways of acrylamide absorption are as follows: inhalation, ingestion, and through the skin and mucous membranes. Basing on a report of WHO from 2002 average daily intake for the general population ranges from 0.3 to 0.8 micrograms of acrylamide per kilogram of body mass. <br />
<br />
Increased consumption of deep-fried food and, subsequently, increased intake of acrylamide, may have serious health consequences. Recent studies have revealed an association between consumption of deep-fried foods and increased prostate cancer risk. What is also suggested, is that there is positive correlation between acrylamide intake and increased risk of endometrial and ovarian cancer. <br />
<br />
Considering the alarming effects of acrylamide exposure, we have decided to include in our project a cytotoxicity study. Our aim is to show how acrylamide may affect various cell lines originating from different tissues. In our experiments we are working on HeLa, 293 and 143B cell lines representing cervical cancer, human embryonic kidney and bone osteosarcoma cells respectively. <br />
<br />
In our experiments we are implementing the following methods: Flow Cytometry, AlamarBlue assay, Cell Growth Assay, Cell Cycle assay and Caspase activity assay.<br />
<br />
To indicate cell viability, we have decided to implement the Alamar Blue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule - resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally, which can be use to measure viability of the cells.<br />
<br />
Flow cytometry enables us to conduct more precise analysis of chosen aspects of cell biology. After conducting flow cytometry, we will be able to state whether treated cells are undergoing apoptosis, necrosis or if the observed metabolic effects are only cytostatic. <br />
<br />
In order to state how acrylamide may influence the progression of cell cycle we have decided to implement Cell Cycle Assay. This method employs flow cytometry to mesure number of cells in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measure the fluorescence. PI binds to DNA quantitatively so the fluorescence intensity of the stained cells is correlated with the amount of their DNA. Thus, basing on the quantity of DNA we are enabled to assign cells to different cell cycle phases. <br />
<br />
We expect to observe the cytotoxic effects in higher acrylamide concentrations i.e. 3-5 mM. In lower concentrations <1 mM may occur the elimination of the toxicant, whose effects are to be examined. <br />
<br />
<u>Bibliography:</u><br />
# Stott-Miller M, Neuhouser ML, Stanford JL; ''Consumption of deep-fried foods and risk of prostate cancer''; Epub 2013 Jan 17.<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A; ''Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes''; Epub 2012 Dec 31<br />
# Ahmad Besaratinia_ and Gerd P.Pfeifer; ''A review of mechanisms of acrylamide carcinogenicity''; Carcinogenesis vol.28 no.3 pp.519–528, 2007<br />
# O'Brien J, Wilson I, Orton T, Pognan F; ''Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity''; Eur J Biochem. 2000 Sep;267(17):5421-6.<br />
# Naoki Koyamaa,b,c, Hiroko Sakamoto a, Mayumi Sakuraba a, Tomoko Koizumi a, Yoshio Takashima a, Makoto Hayashi a, Hiroshi Matsufuji b, Kazuo Yamagata b, Shuichi Masudac, Naohide Kinae c, Masamitsu Honmaa,; ''Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells''; Mutation Research 603 (2006) 151–158<br />
<br />
==Results==<br />
<br />
After conducting our experiments we have obtained following results:<br />
* loss of metabolic activity (results obtained from AlamarBlue assay)<br />
* induction of apoptotic cell death (stated by the presence of annexinV positive cells and the observation of caspase activity)<br />
* suppression of cell growth and proliferation (stated on the basis of Cell Growth assay and AlamarBlue assay)<br />
* disturbances in the cell cycle (based on the outcomes of Cell Cycle assay)<br />
<br />
==Getting the experiments underway==<br />
<br />
As a starting point for our experiments we have decided to implement AlamarBlue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule- resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally and can be use to measure viability of the cells. AlamarBlue assay provides reliable and repeatable results yet is easy to conduct thus it serves as a trustworthy basis for further research. <br />
<br />
In order to obtain reliable results of experiments we have decided to conduct preliminary assays to determine plating density and concentration of acrylamide for each cell line: HeLa, 293 and 143B. The protocol of the assay as well as detailed conduct can be found in the section: Extras.<br />
<br />
The outcome of AlamarBlue assay showed the existence of strong, negative correlation between acrylamide exposure and cell viability (Figure 1.). So the longer exposure time and the higher concentration of acrylamide, the weaker the cell viability. <br />
<br />
[[File:avHEK2.png|550px|frameless|border|alt=A|Figure 1. Influence of acrylamide on 293 cells viability. Cells were treated with various concentration of acrylamide and the viability was measured after indicated period of time.|]]<br />
<br />
In order to state whether the loss of cell viability, that we have observed, was caused by decrease in cell division (the cytostatic effect of acrylamide) or by cell death (the cytotoxic effect of acrylamide) we have implemented Cell growth assays. To conduct the assays we have seeded cells from different cell lines (HeLa, 293 and 143B) in 6-wells plates adding proper concentration of acrylamide to each well and counted the amount of cells in counting chamber after trypan blue staining, for 24, 48 and 72 hours incubation. This approach indicated that acrylamide treatment affects cell proliferation and induces cell death.<br />
[[File:CG2HeLa.png|frameless|border|caption|850px]]<br />
<br />
[[File:CGA-HeLa.png|frameless|border|caption|550px]]<br />
<br />
In order to bring under scrutiny changes in the cell cycle we've carried out Cell Cycle assay. We observed that there occur disturbances in the cell cycle depending on concentration of acrylamide and incubation time.<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Histograms for Cell Cycle assay"><br />
<br />
<br />
File:HeLa-hist.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-hist.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK293-hist.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
<br />
</gallery><br />
<br />
<html><br />
These results made us to consider further investigation. As we wanted to state whether acrylamide induces apoptotic cell death we decided to measure caspase activity and examine presence of the cells that would bind annexinV (which are hallmarks of apoptosis). <br />
<a href="http://pl.promega.com/products/cell-health-and-metabolism/apoptosis-assays/luminescent-caspase-assays/caspase_glo-3_7-assay-systems/?origUrl=http%3a%2f%2fwww.promega.com%2fproducts%2fcell-health-and-metabolism%2fapoptosis-assays%2fluminescent-caspase-assays%2fcaspase_glo-3_7-assay-systems%2f" target="_blank">Caspase-Glo 3/7 Assay</a> revealed that there is an increase in caspase activity caused by acrylamide treatement. <br />
The Flow Cytometry analysis showed that there is a growth in annexinV positive cells number due to addition of acrylamide. <br />
<br />
<br />
</html><br />
<br />
[[File:caspase1.png|frameless|border|caption|450px]]<br />
<br />
<br />
<gallery widths=800px heights=400px perrow=1 caption="Figures for the Flow Cytometry"><br />
<br />
<br />
File:HeLa-FC.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
File:143B-FC.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
<br />
File:HEK-FC.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
<br />
</gallery><br />
<br />
In our study we've included Cisplatin control, whose results are to be seen in section: [https://2013.igem.org/Team:Warsaw/Notebook Notebook]<br />
<br />
All of the results as well as graphs, figures and statistics are to be seen in section: [https://2013.igem.org/Team:Warsaw/Cellular_biology_lab_journal Cellular biology lab journal]<br />
<br />
==Summary==<br />
<br />
Despite the fact that it is impossible to relate directly the results obtained for the cell lines to human organism, it may give a basis for further examination and extended research. In our experiments '''we showed the existence of cytotoxic and cytostatic effect of acrylamide on HeLa, 143B and 293 cell lines. We also found a correlation between time of exposure to acrylamide and the decrease in cell viability and proliferation.''' <br />
<br />
By carrying out our cytotoxicity study '''we wanted to emphasize the importance of synthetic biology research'''. After showing the possible harmful effects of acrylamide we aimed to reveal how products of synthetic biology may influence and facilitate our everyday life. We also tried to raise the awareness of the threats connected with the consumption of tertiary processed foods. <br />
<br />
Finally, let us make you imagine that you are able to check the amount of acrylamide before eating your chips. With our FluoSafe sensor it will be soon possible.<br />
<br />
{{:Team:Warsaw/Templates/StandardPageEnd}}</div>Ahttp://2013.igem.org/File:CG2HeLa.pngFile:CG2HeLa.png2013-10-04T22:54:03Z<p>A: uploaded a new version of &quot;File:CG2HeLa.png&quot;</p>
<hr />
<div></div>Ahttp://2013.igem.org/Team:Warsaw/CytotoxicityTeam:Warsaw/Cytotoxicity2013-10-04T22:49:56Z<p>A: </p>
<hr />
<div>{{:Team:Warsaw/Templates/StandardPageBegin|Cytotoxicity study}}<br />
__TOC__<br />
==Overview==<br />
The neurotoxic effects of acrylamide have been widely known for many years. Local symptoms of acrylamide poisoning such as mucous membrane and skin irritation have been formerly reported. There also exists a systemic effect characterized by fatigue, sleepiness and memory difficulties; in extreme cases also hallucination, disorientation and confusion were observed. <br />
<br />
While acrylamide was perceived as a severe neurotoxin, there had long not been enough studies on its possible carcinogenicity. Yet, the recent research have shown that acrylamide may be engaged in processes. <br />
Due to the rapid development of the plastic industry, acrylamide is becoming even more widely present in our environment. But the manufacturing area is not the only way by which acrylamide appears in our proximity. While deep-frying or baking, through a chemical reaction between an amino acid and a reducing sugar (called the Maillard reaction), acrylamide is formed. <br />
<br />
Possible ways of acrylamide absorption are as follows: inhalation, ingestion, and through the skin and mucous membranes. Basing on a report of WHO from 2002 average daily intake for the general population ranges from 0.3 to 0.8 micrograms of acrylamide per kilogram of body mass. <br />
<br />
Increased consumption of deep-fried food and, subsequently, increased intake of acrylamide, may have serious health consequences. Recent studies have revealed an association between consumption of deep-fried foods and increased prostate cancer risk. What is also suggested, is that there is positive correlation between acrylamide intake and increased risk of endometrial and ovarian cancer. <br />
<br />
Considering the alarming effects of acrylamide exposure, we have decided to include in our project a cytotoxicity study. Our aim is to show how acrylamide may affect various cell lines originating from different tissues. In our experiments we are working on HeLa, 293 and 143B cell lines representing cervical cancer, human embryonic kidney and bone osteosarcoma cells respectively. <br />
<br />
In our experiments we are implementing the following methods: Flow Cytometry, AlamarBlue assay, Cell Growth Assay, Cell Cycle assay and Caspase activity assay.<br />
<br />
To indicate cell viability, we have decided to implement the Alamar Blue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule - resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally, which can be use to measure viability of the cells.<br />
<br />
Flow cytometry enables us to conduct more precise analysis of chosen aspects of cell biology. After conducting flow cytometry, we will be able to state whether treated cells are undergoing apoptosis, necrosis or if the observed metabolic effects are only cytostatic. <br />
<br />
In order to state how acrylamide may influence the progression of cell cycle we have decided to implement Cell Cycle Assay. This method employs flow cytometry to mesure number of cells in a given phase of the cell cycle. Permeabilization of cells with cold ethanol and addition of propidium iodide (PI) prior to analysis, allows us to measure the fluorescence. PI binds to DNA quantitatively so the fluorescence intensity of the stained cells is correlated with the amount of their DNA. Thus, basing on the quantity of DNA we are enabled to assign cells to different cell cycle phases. <br />
<br />
We expect to observe the cytotoxic effects in higher acrylamide concentrations i.e. 3-5 mM. In lower concentrations <1 mM may occur the elimination of the toxicant, whose effects are to be examined. <br />
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<u>Bibliography:</u><br />
# Stott-Miller M, Neuhouser ML, Stanford JL; ''Consumption of deep-fried foods and risk of prostate cancer''; Epub 2013 Jan 17.<br />
# Ehlers A, Lenze D, Broll H, Zagon J, Hummel M, Lampen A; ''Dose dependent molecular effects of acrylamide and glycidamide in human cancer cell lines and human primary hepatocytes''; Epub 2012 Dec 31<br />
# Ahmad Besaratinia_ and Gerd P.Pfeifer; ''A review of mechanisms of acrylamide carcinogenicity''; Carcinogenesis vol.28 no.3 pp.519–528, 2007<br />
# O'Brien J, Wilson I, Orton T, Pognan F; ''Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity''; Eur J Biochem. 2000 Sep;267(17):5421-6.<br />
# Naoki Koyamaa,b,c, Hiroko Sakamoto a, Mayumi Sakuraba a, Tomoko Koizumi a, Yoshio Takashima a, Makoto Hayashi a, Hiroshi Matsufuji b, Kazuo Yamagata b, Shuichi Masudac, Naohide Kinae c, Masamitsu Honmaa,; ''Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells''; Mutation Research 603 (2006) 151–158<br />
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==Results==<br />
<br />
After conducting our experiments we have obtained following results:<br />
* loss of metabolic activity (results obtained from AlamarBlue assay)<br />
* induction of apoptotic cell death (stated by the presence of annexinV positive cells and the observation of caspase activity)<br />
* suppression of cell growth and proliferation (stated on the basis of Cell Growth assay and AlamarBlue assay)<br />
* disturbances in the cell cycle (based on the outcomes of Cell Cycle assay)<br />
<br />
==Getting the experiments underway==<br />
<br />
As a starting point for our experiments we have decided to implement AlamarBlue assay. It bases on the ability of living cells to convert nonfluorescent resazurin to the highly fluorescent molecule- resorufin. While entering the cells, resazurin is being reduced to resorufin and the bright, red fluorescence is observed. Viable cells are continuing to convert resazurin to resorufin, so the fluorescence intensity increases proportionally and can be use to measure viability of the cells. AlamarBlue assay provides reliable and repeatable results yet is easy to conduct thus it serves as a trustworthy basis for further research. <br />
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In order to obtain reliable results of experiments we have decided to conduct preliminary assays to determine plating density and concentration of acrylamide for each cell line: HeLa, 293 and 143B. The protocol of the assay as well as detailed conduct can be found in the section: Extras.<br />
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The outcome of AlamarBlue assay showed the existence of strong, negative correlation between acrylamide exposure and cell viability (Figure 1.). So the longer exposure time and the higher concentration of acrylamide, the weaker the cell viability. <br />
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[[File:avHEK2.png|550px|frameless|border|Figure 1. Influence of acrylamide on 293 cells viability. Cells were treated with various concentration of acrylamide and the viability was measured after indicated period of time.|]]<br />
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In order to state whether the loss of cell viability, that we have observed, was caused by decrease in cell division (the cytostatic effect of acrylamide) or by cell death (the cytotoxic effect of acrylamide) we have implemented Cell growth assays. To conduct the assays we have seeded cells from different cell lines (HeLa, 293 and 143B) in 6-wells plates adding proper concentration of acrylamide to each well and counted the amount of cells in counting chamber after trypan blue staining, for 24, 48 and 72 hours incubation. This approach indicated that acrylamide treatment affects cell proliferation and induces cell death.<br />
[[File:CG2HeLa.png|frameless|border|caption|850px]]<br />
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[[File:CGA-HeLa.png|frameless|border|caption|550px]]<br />
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In order to bring under scrutiny changes in the cell cycle we've carried out Cell Cycle assay. We observed that there occur disturbances in the cell cycle depending on concentration of acrylamide and incubation time.<br />
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<gallery widths=800px heights=400px perrow=1 caption="Histograms for Cell Cycle assay"><br />
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<br />
File:HeLa-hist.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
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File:143B-hist.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
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File:HEK293-hist.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
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</gallery><br />
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<html><br />
These results made us to consider further investigation. As we wanted to state whether acrylamide induces apoptotic cell death we decided to measure caspase activity and examine presence of the cells that would bind annexinV (which are hallmarks of apoptosis). <br />
<a href="http://pl.promega.com/products/cell-health-and-metabolism/apoptosis-assays/luminescent-caspase-assays/caspase_glo-3_7-assay-systems/?origUrl=http%3a%2f%2fwww.promega.com%2fproducts%2fcell-health-and-metabolism%2fapoptosis-assays%2fluminescent-caspase-assays%2fcaspase_glo-3_7-assay-systems%2f" target="_blank">Caspase-Glo 3/7 Assay</a> revealed that there is an increase in caspase activity caused by acrylamide treatement. <br />
The Flow Cytometry analysis showed that there is a growth in annexinV positive cells number due to addition of acrylamide. <br />
<br />
<br />
</html><br />
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[[File:caspase1.png|frameless|border|caption|450px]]<br />
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<gallery widths=800px heights=400px perrow=1 caption="Figures for the Flow Cytometry"><br />
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<br />
File:HeLa-FC.png|HeLa cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
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File:143B-FC.png|143B cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 2mM, 5mM<br />
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File:HEK-FC.png|HEK293 cell line upper row: 24h incubation bottom row: 48h incubation acrylamide concentrations from left: NC, 1mM, 3mM, 5mM<br />
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</gallery><br />
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In our study we've included Cisplatin control, whose results are to be seen in section: [https://2013.igem.org/Team:Warsaw/Notebook Notebook]<br />
<br />
All of the results as well as graphs, figures and statistics are to be seen in section: [https://2013.igem.org/Team:Warsaw/Cellular_biology_lab_journal Cellular biology lab journal]<br />
<br />
==Summary==<br />
<br />
Despite the fact that it is impossible to relate directly the results obtained for the cell lines to human organism, it may give a basis for further examination and extended research. In our experiments '''we showed the existence of cytotoxic and cytostatic effect of acrylamide on HeLa, 143B and 293 cell lines. We also found a correlation between time of exposure to acrylamide and the decrease in cell viability and proliferation.''' <br />
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By carrying out our cytotoxicity study '''we wanted to emphasize the importance of synthetic biology research'''. After showing the possible harmful effects of acrylamide we aimed to reveal how products of synthetic biology may influence and facilitate our everyday life. We also tried to raise the awareness of the threats connected with the consumption of tertiary processed foods. <br />
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Finally, let us make you imagine that you are able to check the amount of acrylamide before eating your chips. With our FluoSafe sensor it will be soon possible.<br />
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