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http://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab/Modelling
Team:Ciencias-UNAM/Project/DryLab/Modelling
2013-09-28T04:22:04Z
<p>Dheredia: </p>
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<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Modelling</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<br />
The final aim of our project involves population dynamics between <i>Skully coli</i> and <i>H. pylori</i>. Modelling the basic interactions in this system is very useful in a first instance to calibrate the induction of our system. This balance is crucial in our dynamic. Since we intend to produce an antimicrobial peptide <i>in vivo</i> and we are using a peptide that is inherently toxic to the host, we need to be able to calibrate the production of the peptide and the efficiency of the resistance in order to have a working system.<br />
<br />
The model is based on differential equations describing the population of <i>Skully coli</i> <b> S</b>and of <i>Helicobacter pylori</i> <b> H</b>as well as the production of the antimicrobial peptide <b> P</b>.<br />
<br><br />
Please check our Modelling section to see the detailed model.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Achievements
Team:Ciencias-UNAM/Achievements
2013-09-28T04:18:16Z
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Achievements</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> Please visit our <b><a href="https://igem.org/2013_Judging_Form?id=1230">Judging Form</a> </b><br />
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<br><b>Requirements for a Bronze Medal:</b><br />
<br>Register the team, have a great summer, and plan to have fun at the Regional Jamboree. <br />
<br>Successfully complete and submit this iGEM 2013 Judging form.<br />
<br>Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.<br />
<br>Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br>Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). <br />
<br>https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks <br />
<br />
<br><br><b>Additional Requirements for a Silver Medal:</b><br />
<br>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br>Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br>Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
<br><br><b>Additional Requirements for a Gold Medal:</b><br />
<br>Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230007<br />
<br>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br>We did formal Petition to Mexican Congress to review DNA import/export politics, addressing a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress, given that in Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), regulates, with a lot of normative has flaws, both imports and export of genetic material for research. These flaws make genetic material imports unavailable for very large periods of time. Noting the urge of creating a law initiative in this matter and proposing changes in the already existing norms, we take in account aspects such as security, ethics, and above all, the aspect of sharing. <br />
<br><br />
<br><b>Best BioBrick Measurement Approach: </b><br />
<br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.<br />
<br />
<br><b>Best Model:</b> We were able to recognize parameters based on the system dynamics. <br />
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<br><b>Team_Parts</b><br />
<br>Best new BioBrick Part, Natural:<br />
<br>Part Number(s): BBa_K1230000, “AcrAB-TolC multidrug efflux transport system activator from E. coli”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230000<br />
<br>Best new BioBrick Part or Device, Engineered:<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br />
<br><b>Most Improved Registry Part:</b><br />
<br><br><i>Part Number(s):</i> BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br><br><i>Short description of improvements made to the part:</i><br />
<br><br>We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br><b>Best Part Collection:</b> (give the range of part numbers)<br />
<br><br><i>Part Number(s): </i>BBa_K1230000, BBa_K1230001, BBa_K1230004, BBa_K1230007, BBa_K1230009, BBa_K1230010, BBa_K1230011, BBa_K1230013, BBa_K1230015, BBa_K12300016 not found<br />
<br><br><i>Short description of collection function:</i><br />
<br><br>As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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Dheredia
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Team:Ciencias-UNAM/Achievements
2013-09-28T04:17:00Z
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Achievements</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> Please visit our <b><a href="https://static.igem.org/mediawiki/igem.org/d/d9/U62_Mario.jpg">Judging Form</a> <b><br />
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<br><b>Requirements for a Bronze Medal:</b><br />
<br>Register the team, have a great summer, and plan to have fun at the Regional Jamboree. <br />
<br>Successfully complete and submit this iGEM 2013 Judging form.<br />
<br>Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.<br />
<br>Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br>Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). <br />
<br>https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks <br />
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<br><br><b>Additional Requirements for a Silver Medal:</b><br />
<br>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br>Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br>Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
<br><br><b>Additional Requirements for a Gold Medal:</b><br />
<br>Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230007<br />
<br>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br>We did formal Petition to Mexican Congress to review DNA import/export politics, addressing a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress, given that in Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), regulates, with a lot of normative has flaws, both imports and export of genetic material for research. These flaws make genetic material imports unavailable for very large periods of time. Noting the urge of creating a law initiative in this matter and proposing changes in the already existing norms, we take in account aspects such as security, ethics, and above all, the aspect of sharing. <br />
<br><br />
<br><b>Best BioBrick Measurement Approach: </b><br />
<br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.<br />
<br />
<br><b>Best Model:</b> We were able to recognize parameters based on the system dynamics. <br />
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<br><b>Team_Parts</b><br />
<br>Best new BioBrick Part, Natural:<br />
<br>Part Number(s): BBa_K1230000, “AcrAB-TolC multidrug efflux transport system activator from E. coli”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230000<br />
<br>Best new BioBrick Part or Device, Engineered:<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
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<br><b>Most Improved Registry Part:</b><br />
<br><br><i>Part Number(s):</i> BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br><br><i>Short description of improvements made to the part:</i><br />
<br><br>We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br><b>Best Part Collection:</b> (give the range of part numbers)<br />
<br><br><i>Part Number(s): </i>BBa_K1230000, BBa_K1230001, BBa_K1230004, BBa_K1230007, BBa_K1230009, BBa_K1230010, BBa_K1230011, BBa_K1230013, BBa_K1230015, BBa_K12300016 not found<br />
<br><br><i>Short description of collection function:</i><br />
<br><br>As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_InstructorsAdvisors
Team:Ciencias-UNAM/stylesheets/specificstyle InstructorsAdvisors
2013-09-28T04:12:13Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Team/Instructors_Advisors
Team:Ciencias-UNAM/Team/Instructors Advisors
2013-09-28T04:10:28Z
<p>Dheredia: </p>
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Advisors</span></p></div><br />
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<div id="u58_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Pablo Padilla Longoria</span> <br><br />
<span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;"><i>Instructor</i></span><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<b>Hometown:</b> Ciudad de México, México D.F.<br><br />
<b>Education:</b> Mathematics (Facultad de Ciencias, UNAM), Physics (Facultad de Ciencias, UNAM), Piano (Mannes College of Music, CUNY), M.S. in Mathematics (Courant Institute of Mathematical Sciences, NYU), Ph.D. in Mathematics (Courant Institute of Mathematical Sciences, NYU)<br><br />
<b>Professional interests:</b> Differential Equations, Nonlinear Analysis<br><br />
<b>Attributions:</b> He's the director of the Synthetic Biology Workshop in which we got involved in Synthetic Biology. Financing of the project. DryLab.<br><br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></div><br />
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<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Viviana Escobar Sánchez</span><br><br />
<span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;"><i>Instructor</i></span><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<b>Hometown:</b> Ciudad de México, México D.F.<br><br />
<b>Education:</b> Chemistry (Facultad de Química, UNAM), Ph.D. candidate in Biochemical Sciences (Instituto de Biotecnología, UNAM)<br><br />
<b>Professional interests:</b> Molecular Biology, Molecular Evolution, Structural Biology<br><br />
<b>Attributions:</b> She's professor of the Synthetic Biology Workshop. Worked with us during the whole development of the WetLab in the design and execution of the experiments and allowed us to work in her Lab space.<br><br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></div><br />
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<div id="u64_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Mario</span><br><br />
<span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;"><i>Advisor</i></span><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<b>Hometown:</b> Ciudad de México, México D.F.<br><br />
<b>Education:</b> Mathematics (x)<br><br />
<b>Professional interests:</b> Synthetic Biology <br><br />
<b>Attributions:</b> He helped us with the development of the model and the characterization.<br><br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Achievements
Team:Ciencias-UNAM/Achievements
2013-09-28T04:08:17Z
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Achievements</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br> We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
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The principal aim of our project was to collaborate as much as possible to the iGEM mission to develop science freely. The parts we designed followed this principle, by being useful in very aspects, apart of our own project. We developed a Multiple Antibiotic Resistance protein, which acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1. This part can be used in several ways in different iGEM projects. We also sent to the parts registry the LL-37 peptide, with adequate codon usage, an improvement on part BBa_K875000, that allows LL-37 expression. <br><br><br />
As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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<br>Requirements for a Bronze Medal:<br />
<br>Register the team, have a great summer, and plan to have fun at the Regional Jamboree. <br />
<br>Successfully complete and submit this iGEM 2013 Judging form.<br />
<br>Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.<br />
<br>Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br>Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). <br />
<br>https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks <br />
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<br><br>Additional Requirements for a Silver Medal:<br />
<br>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br>Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br>Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
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<br><br>Additional Requirements for a Gold Medal:<br />
<br>Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230007<br />
<br>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br>We did formal Petition to Mexican Congress to review DNA import/export politics, addressing a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress, given that in Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), regulates, with a lot of normative has flaws, both imports and export of genetic material for research. These flaws make genetic material imports unavailable for very large periods of time. Noting the urge of creating a law initiative in this matter and proposing changes in the already existing norms, we take in account aspects such as security, ethics, and above all, the aspect of sharing. <br />
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<br>Best BioBrick Measurement Approach: <br />
<br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.<br />
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<br>Best Model: We were able to recognize parameters based on the system dynamics. <br />
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<br>Team_Parts<br />
<br>Best new BioBrick Part, Natural:<br />
<br>Part Number(s): BBa_K1230000, “AcrAB-TolC multidrug efflux transport system activator from E. coli”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230000<br />
<br>Best new BioBrick Part or Device, Engineered:<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
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<br>Most Improved Registry Part:<br />
<br><br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br><br>Short description of improvements made to the part:<br />
<br><br>We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br>Best Part Collection: (give the range of part numbers)<br />
<br><br>Part Number(s): BBa_K1230000, BBa_K1230001, BBa_K1230004, BBa_K1230007, BBa_K1230009, BBa_K1230010, BBa_K1230011, BBa_K1230013, BBa_K1230015, BBa_K12300016 not found<br />
<br><br>Short description of collection function:<br />
<br><br>As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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Dheredia
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Team:Ciencias-UNAM/Achievements
2013-09-28T04:05:42Z
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<div id="u46_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Home &gt; Achievements</span></p></div><br />
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Achievements</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br> We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br><br />
The principal aim of our project was to collaborate as much as possible to the iGEM mission to develop science freely. The parts we designed followed this principle, by being useful in very aspects, apart of our own project. We developed a Multiple Antibiotic Resistance protein, which acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1. This part can be used in several ways in different iGEM projects. We also sent to the parts registry the LL-37 peptide, with adequate codon usage, an improvement on part BBa_K875000, that allows LL-37 expression. <br><br><br />
As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
<br />
<br>Requirements for a Bronze Medal:<br />
<br>Register the team, have a great summer, and plan to have fun at the Regional Jamboree. <br />
<br>Successfully complete and submit this iGEM 2013 Judging form.<br />
<br>Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.<br />
<br>Plan to present a Poster and Talk at the iGEM Jamboree.<br />
<br>Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). <br />
<br>https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks <br />
<br />
<br><br>Additional Requirements for a Silver Medal:<br />
<br>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.<br />
<br>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry<br />
<br>Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br>Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.<br />
<br />
<br><br>Additional Requirements for a Gold Medal:<br />
<br>Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the 'Experience' section of that Parts's Registry entry), create a new registry page for the improved part, and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines).<br />
<br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230007<br />
<br>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.<br />
<br>We did formal Petition to Mexican Congress to review DNA import/export politics, addressing a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress, given that in Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), regulates, with a lot of normative has flaws, both imports and export of genetic material for research. These flaws make genetic material imports unavailable for very large periods of time. Noting the urge of creating a law initiative in this matter and proposing changes in the already existing norms, we take in account aspects such as security, ethics, and above all, the aspect of sharing. <br />
<br />
<br>Best BioBrick Measurement Approach: <br />
<br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.<br />
<br />
<br>Best Model:<br />
<br />
<br>Team_Parts<br />
<br>Best new BioBrick Part, Natural:<br />
<br>Part Number(s): BBa_K1230000, “AcrAB-TolC multidrug efflux transport system activator from E. coli”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230000<br />
<br>Best new BioBrick Part or Device, Engineered:<br />
<br>Part Number(s): BBa_K1230004, “MarA Generator”<br />
<br>http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004<br />
<br />
<br>Most Improved Registry Part:<br />
<br><br>Part Number(s): BBa_K1230007, “araC-pBad-rbs-LL37”<br />
<br><br>Short description of improvements made to the part:<br />
<br><br>We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br>Best Part Collection: (give the range of part numbers)<br />
<br><br>Part Number(s): BBa_K1230000, BBa_K1230001, BBa_K1230004, BBa_K1230007, BBa_K1230009, BBa_K1230010, BBa_K1230011, BBa_K1230013, BBa_K1230015, BBa_K12300016 not found<br />
<br><br>Short description of collection function:<br />
<br><br>As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
<br />
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Dheredia
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Team:Ciencias-UNAM/scripts/pagescript Home
2013-09-28T04:05:00Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Modeling
Team:Ciencias-UNAM/stylesheets/specificstyle Modeling
2013-09-28T04:02:40Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab/Modelling
Team:Ciencias-UNAM/Project/DryLab/Modelling
2013-09-28T04:01:24Z
<p>Dheredia: </p>
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<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Modelling</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<br />
The final aim of our project involves population dynamics between <i>Skully coli</i> and <i>H. pylori</i>. Modelling the basic interactions in this system is very useful in a first instance to calibrate the induction of our system. This balance is crucial in our dynamic. Since we intend to produce an antimicrobial peptide <i>in vivo</i> and we are using a peptide that is inherently toxic to the host, we need to be able to calibrate the production of the peptide and the efficiency of the resistance in order to have a working system.<br />
<br />
The model is based on differential equations describing the population of <i>Skully coli</i> <b> S</b>and of <i>Helicobacter pylori</i> <b> H</b>as well as the production of the antimicrobial peptide <b> P</b>.<br />
<br><br />
Please check our Modelling section to see the detailed model.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p></div><br />
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http://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab/Modelling
Team:Ciencias-UNAM/Project/DryLab/Modelling
2013-09-28T03:59:08Z
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<br />
The final aim of our project involves population dynamics between <i>Skully coli</i> and <i>H. pylori</i>. Modelling the basic interactions in this system is very useful in a first instance to calibrate the induction of our system. This balance is crucial in our dynamic. Since we intend to produce an antimicrobial peptide <i>in vivo</i> and we are using a peptide that is inherently toxic to the host, we need to be able to calibrate the production of the peptide and the efficiency of the resistance in order to have a working system.<br />
<br />
The model is based on differential equations describing the population of <i>Skully coli</i> <b> S</b>and of <i>Helicobacter pylori</i> <b> H</b>as well as the production of the antimicrobial peptide <b> P</b>.<br />
<br><br />
Please check our Modelling section to see the detailed model.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p></div><br />
<br />
<div id="u70" class="u70_container" ><br />
<br />
<div id="u70_img" > </div><br />
<br />
<img src="https://static.igem.org/mediawiki/2013/0/0a/Modelo_igem_2013.png" class="raw_image"></div> <br />
</div><br />
</div><br />
<div class="preload"><br />
<img src="https://static.igem.org/mediawiki/2013/d/d1/Bg_ciencias.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/5/59/U0_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/c/c3/U2_menu.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/9/96/U4_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/8/84/U8_mouseOver.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/6/6f/U8_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/c/ca/U12_mouseOver.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/e/e3/U12_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/0/0a/U14_menu.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/5/55/U28_menu.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/6/68/U30_mouseOver.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/f/f7/U30_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/6/6c/U34_menu.png" width="1" height="1"/><br />
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<img src="https://static.igem.org/mediawiki/2013/0/0a/U42_normal.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/e/e3/U73_line.png" width="1" height="1"/><br />
<img src="https://static.igem.org/mediawiki/2013/0/0a/Modelo_igem_2013.png" width="1" height="1"/><br />
</div><br />
</body><br />
<br />
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript?action=raw&amp;ctype=text/javascript"></script><br />
<br />
<script src="https://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript_Modeling?action=raw&amp;ctype=text/javascript" charset="utf-8"></script><br />
</html></div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Modeling
Team:Ciencias-UNAM/stylesheets/specificstyle Modeling
2013-09-28T03:58:48Z
<p>Dheredia: </p>
<hr />
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}<br />
<br />
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display: none;<br />
}<br />
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}<br />
<br />
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;<br />
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;<br />
;<br />
;<br />
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;<br />
;<br />
;<br />
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<br />
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}<br />
<br />
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<br />
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;<br />
;<br />
;<br />
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Dheredia
http://2013.igem.org/File:Modelo_igem_2013.png
File:Modelo igem 2013.png
2013-09-28T03:52:42Z
<p>Dheredia: </p>
<hr />
<div></div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM
Team:Ciencias-UNAM
2013-09-28T03:46:04Z
<p>Dheredia: </p>
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<div id="u54_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">This year's project is an <i>In vivo</i> production of an antimicrobial peptide regulated by <i>quorum sensing</i>. We designed a synthetic system in <i>E. coli</i> to express an antimicrobial peptide (Cathelicidin LL-37) <i>in vivo</i> only in the presence of a quorum sensing molecule (AI-2) and expel it with an efflux pump in order to act against targeted gastrointestinal pathogens.</span></p></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/Conclusions
Team:Ciencias-UNAM/Project/Conclusions
2013-09-28T03:38:57Z
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Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. To measure the experiment in this way is relevant, since the expected function of the part is a phenotype. Although measuring via reporters is useful to infere the production of a certain protein, the cuatitiative measure of the effect is more relevant in parts like ours. The designed experiments allow to use two variables (in this case the induction with IPTG and the concentration of antibiotic) and to show not only the characterization of a promoter (as it is often done) but also a cuantitative measure of the desired phenotype.<br />
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self.location.href='https://2013.igem.org/Main_Page';<br />
<br />
}<br />
});<br />
gv_vAlignTable['u47'] = 'top';gv_vAlignTable['u49'] = 'top';gv_vAlignTable['u1'] = 'center';document.getElementById('u4_img').tabIndex = 0;<br />
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<br />
u4.style.cursor = 'pointer';<br />
$axure.eventManager.click('u4', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u5'] = 'center';HookHover('u6', false);<br />
gv_vAlignTable['u7'] = 'center';HookHover('u8', false);<br />
gv_vAlignTable['u9'] = 'center';gv_vAlignTable['u51'] = 'top';gv_vAlignTable['u53'] = 'top';gv_vAlignTable['u54'] = 'top';gv_vAlignTable['u55'] = 'top';gv_vAlignTable['u56'] = 'top';gv_vAlignTable['u57'] = 'top';u58.tabIndex = 0;<br />
<br />
u58.style.cursor = 'pointer';<br />
$axure.eventManager.click('u58', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u58'] = 'top';u59.tabIndex = 0;<br />
<br />
u59.style.cursor = 'pointer';<br />
$axure.eventManager.click('u59', function(e) {<br />
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if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u59'] = 'top';u60.tabIndex = 0;<br />
<br />
u60.style.cursor = 'pointer';<br />
$axure.eventManager.click('u60', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks');<br />
<br />
}<br />
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u61.style.cursor = 'pointer';<br />
$axure.eventManager.click('u61', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/Achievements');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u61'] = 'top';gv_vAlignTable['u63'] = 'center';gv_vAlignTable['u65'] = 'center';gv_vAlignTable['u67'] = 'center';gv_vAlignTable['u69'] = 'center';gv_vAlignTable['u71'] = 'center';gv_vAlignTable['u73'] = 'center';gv_vAlignTable['u75'] = 'top';gv_vAlignTable['u76'] = 'top';gv_vAlignTable['u79'] = 'center';</div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Results
Team:Ciencias-UNAM/stylesheets/specificstyle Results
2013-09-28T03:35:42Z
<p>Dheredia: </p>
<hr />
<div>body {<br />
margin: 0px;<br />
background-color: #FFFFFF;<br />
background-image: url('https://static.igem.org/mediawiki/2013/d/d1/Bg_ciencias.png');<br />
background-position: left top;<br />
background-repeat: repeat;<br />
text-align: center;<br />
}<br />
<br />
div {<br />
background-repeat: no-repeat;<br />
}<br />
<br />
.raw_image {<br />
width: 100%;<br />
height: 100%;<br />
}<br />
<br />
.preload {<br />
display: none;<br />
}<br />
#main_container {<br />
}<br />
<br />
.form_sketch {<br />
border-color: transparent;<br />
background-color: transparent;<br />
}<br />
<br />
.u0_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/5/59/U0_normal.png');<br />
}<br />
<br />
.u0_container {<br />
position:absolute;<br />
left:160px;<br />
top:190px;<br />
width:940px;<br />
height:1650px;<br />
}<br />
<br />
#u0_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:946px;<br />
height:1620px;<br />
}<br />
<br />
.u1 {<br />
position:absolute;<br />
left:2px;<br />
top:717px;<br />
width:936px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/c/c3/U2_menu.png');<br />
}<br />
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position:absolute;<br />
left:157px;<br />
top:127px;<br />
width:949px;<br />
height:69px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:946px;<br />
height:66px;<br />
}<br />
<br />
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position:absolute;<br />
left:2px;<br />
top:2px;<br />
width:941px;<br />
height:61px<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/9/96/U4_normal.png');<br />
}<br />
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top:0px;<br />
width:150px;<br />
height:56px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:156px;<br />
height:62px;<br />
}<br />
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position:absolute;<br />
left:2px;<br />
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width:146px;<br />
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;<br />
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;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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}<br />
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top:0px;<br />
width:150px;<br />
height:56px;<br />
}<br />
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top:-3px;<br />
width:156px;<br />
height:62px;<br />
}<br />
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;<br />
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text-align:left;<br />
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left:300px;<br />
top:0px;<br />
width:250px;<br />
height:56px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:256px;<br />
height:62px;<br />
}<br />
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position:absolute;<br />
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width:246px;<br />
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;<br />
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}<br />
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;<br />
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}<br />
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}<br />
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;<br />
;<br />
;<br />
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width:159px;<br />
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}<br />
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}<br />
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}<br />
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}<br />
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}<br />
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}<br />
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}<br />
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;<br />
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}<br />
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}<br />
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}<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/Results
Team:Ciencias-UNAM/Project/WetLab/Results
2013-09-28T03:34:13Z
<p>Dheredia: </p>
<hr />
<div><html><br />
<head><br />
<title>Results</title><br />
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<div id="u5_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HOME</span></p></div><br />
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<br />
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<div id="u63_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> <br><br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. (For details on the protocols, experiment design and photographs pelase refer to the Notebook).<br />
The measurement for this in all the experiments listed below, was calculated by measuring the diameter of the inhibition circles in the plates, calculating the area and substracting the area of the inhibition disc.<br />
<br><br><br />
For the characterization of MarA we used the MarA generator (BBa_K1230004), which is under the Lac promoter. Our first aproach was to test the part with Tetracycline. The results of the experiment are shown in <b>Figure 1</b>. The results show no difference with the differential induction of concentrations of IPTG, but it shows a general interesting behaviour. The cultures transformed with the plasmid show a higher sensitivity to the antibiotic. The control can be observed (in orange) which is a common strain of DH5α.<br />
<br><br><br />
Due to this unexpected result, we decided to do a second experiment, this time using again Tetracyclin, and also Kanamycin and Ampicilin. The results for the same type of experiment (see Notebook) are shown in <b>Figure 2</b>. This experiment confirmed the results obtained in the first experiment. There was no clear difference between the control and the experimental cultures for Ampicilin, and a slight difference with Kanamycin.<br />
<br><br><br />
Since Kanamycin showed the most promising results, a third experiment was made using a broader range of this antibiotic. This results are shown in <b>Figure 3.</b> <br />
<br><br><br />
Again, this figure shows no difference between the IPTG levels, but it shows a very clear difference between the bacteria with the plasmid and the control. This shows an overall increased resistance against Kanamycin.<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/Results
Team:Ciencias-UNAM/Project/WetLab/Results
2013-09-28T03:28:07Z
<p>Dheredia: </p>
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<title>Results</title><br />
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<div id="u63_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> <br><br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. (For details on the protocols, experiment design and photographs pelase refer to the Notebook).<br />
The measurement for this in all the experiments listed below, was calculated by measuring the diameter of the inhibition circles in the plates, calculating the area and substracting the area of the inhibition disc.<br />
<br><br><br />
For the characterization of MarA we used the MarA generator (BBa_K1230004), which is under the Lac promoter. Our first aproach was to test the part with Tetracycline. The results of the experiment are shown in <b>Figure 1</b>. The results show no difference with the differential induction of concentrations of IPTG, but it shows a general interesting behaviour. The cultures transformed with the plasmid show a higher sensitivity to the antibiotic. The control can be observed (in orange) which is a common strain of DH5α.<br />
<br><br><br />
Due to this unexpected result, we decided to do a second experiment, this time using again Tetracyclin, and also Kanamycin and Ampicilin. The results for the same type of experiment (see Notebook) are shown in <b>Figure 2</b>. This experiment confirmed the results obtained in the first experiment. There was no clear difference between the control and the experimental cultures for Ampicilin, and a slight difference with Kanamycin.<br />
<br><br><br />
Since Kanamycin showed the most promising results, a third experiment was made using a broader range of this antibiotic. This results are shown in <b>Figure 3.</b> <br />
<br><br><br />
Again, this figure shows no difference between the IPTG levels, but it shows a very clear difference between the bacteria with the plasmid and the control. This shows an overall increased resistance against Kanamycin.<br />
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<div id="u63_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> <br><br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. (For details on the protocols, experiment design and photographs pelase refer to the Notebook).<br />
The measurement for this in all the experiments listed below, was calculated by measuring the diameter of the inhibition circles in the plates, calculating the area and substracting the area of the inhibition disc.<br />
<br><br><br />
For the characterization of MarA we used the MarA generator (BBa_K1230004), which is under the Lac promoter. Our first aproach was to test the part with Tetracycline. The results of the experiment are shown in <b>Figure 1</b>. The results show no difference with the differential induction of concentrations of IPTG, but it shows a general interesting behaviour. The cultures transformed with the plasmid show a higher sensitivity to the antibiotic. The control can be observed (in orange) which is a common strain of DH5α.<br />
<br><br><br />
Due to this unexpected result, we decided to do a second experiment, this time using again Tetracyclin, and also Kanamycin and Ampicilin. The results for the same type of experiment (see Notebook) are shown in <b>Figure 2</b>. This experiment confirmed the results obtained in the first experiment. There was no clear difference between the control and the experimental cultures for Ampicilin, and a slight difference with Kanamycin.<br />
<br><br><br />
Since Kanamycin showed the most promising results, a third experiment was made using a broader range of this antibiotic. This results are shown in <b>Figure 3.</b> <br />
<br><br><br />
Again, this figure shows no difference between the IPTG levels, but it shows a very clear difference between the bacteria with the plasmid and the control. This shows an overall increased resistance against Kanamycin.<br />
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<div id="u63_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> <br><br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. (For details on the protocols, experiment design and photographs pelase refer to the Notebook).<br />
The measurement for this in all the experiments listed below, was calculated by measuring the diameter of the inhibition circles in the plates, calculating the area and substracting the area of the inhibition disc.<br />
<br><br><br />
For the characterization of MarA we used the MarA generator (BBa_K1230004), which is under the Lac promoter. Our first aproach was to test the part with Tetracycline. The results of the experiment are shown in <b>Figure 1</b>. The results show no difference with the differential induction of concentrations of IPTG, but it shows a general interesting behaviour. The cultures transformed with the plasmid show a higher sensitivity to the antibiotic. The control can be observed (in orange) which is a common strain of DH5α.<br />
<br><br><br />
Due to this unexpected result, we decided to do a second experiment, this time using again Tetracyclin, and also Kanamycin and Ampicilin. The results for the same type of experiment (see Notebook) are shown in <b>Figure 2</b>. This experiment confirmed the results obtained in the first experiment. There was no clear difference between the control and the experimental cultures for Ampicilin, and a slight difference with Kanamycin.<br />
<br><br><br />
Since Kanamycin showed the most promising results, a third experiment was made using a broader range of this antibiotic. This results are shown in <b>Figure 3.</b> <br />
<br><br><br />
Again, this figure shows no difference between the IPTG levels, but it shows a very clear difference between the bacteria with the plasmid and the control. This shows an overall increased resistance against Kanamycin.<br />
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<div id="u63_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> <br><br>Our experiments focused on the measurements of the expected phenotypes, which in our case, involve the increase or decrease of viability or inhibition. (For details on the protocols, experiment design and photographs pelase refer to the Notebook).<br />
The measurement for this in all the experiments listed below, was calculated by measuring the diameter of the inhibition circles in the plates, calculating the area and substracting the area of the inhibition disc.<br />
<br><br><br />
For the characterization of MarA we used the MarA generator (BBa_K1230004), which is under the Lac promoter. Our first aproach was to test the part with Tetracycline. The results of the experiment are shown in <b>Figure 1</b>. The results show no difference with the differential induction of concentrations of IPTG, but it shows a general interesting behaviour. The cultures transformed with the plasmid show a higher sensitivity to the antibiotic. The control can be observed (in orange) which is a common strain of DH5α.<br />
<br><br><br />
Due to this unexpected result, we decided to do a second experiment, this time using again Tetracyclin, and also Kanamycin and Ampicilin. The results for the same type of experiment (see Notebook) are shown in <b>Figure 2</b>. This experiment confirmed the results obtained in the first experiment. There was no clear difference between the control and the experimental cultures for Ampicilin, and a slight difference with Kanamycin.<br />
<br><br><br />
Since Kanamycin showed the most promising results, a third experiment was made using a broader range of this antibiotic. This results are shown in <b>Figure 3.</b> <br />
<br><br><br />
Again, this figure shows no difference between the IPTG levels, but it shows a very clear difference between the bacteria with the plasmid and the control. This shows an overall increased resistance against Kanamycin.<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/Protocols
Team:Ciencias-UNAM/Project/WetLab/Protocols
2013-09-28T03:02:28Z
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<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Notebook</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">If you're having problems visualizing our notebook you can see it <a href="http://www.issuu.com/Ciencias-UNAM/docs/wetlab_notebookpdf?e=9426086/4999752">here</a></p></div> </div><br />
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Team:Ciencias-UNAM/Project/WetLab/Protocols
2013-09-28T02:58:30Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/Protocols
Team:Ciencias-UNAM/Project/WetLab/Protocols
2013-09-28T02:56:02Z
<p>Dheredia: </p>
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<div id="u49_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">General Design</span></p></div><br />
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<div id="u50_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Wet Lab</span></p></div><br />
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Results</span></p></div><br />
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<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Characterization</span></p></div><br />
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<div id="u54_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;"> Notebook</span></p></div><br />
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<div id="u55_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Dry Lab</span></p></div><br />
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<div id="u56_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Modelling</span></p></div><br />
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<div id="u57_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Conclusions </span></p></div><br />
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<div id="u58_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Future Work</span></p></div><br />
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<div id="u59" class="u59" ><br />
<div id="u59_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">References</span></p></div><br />
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<DIV id="u60_line" class="u60_line" ></DIV><br />
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<div id="u61" class="u61" ><br />
<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Notebook</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">If you're having problems visualizing our notebook you can see it <a href="issuu.com/Ciencias-UNAM/docs/wetlab_notebookpdf?e=9426086/4999752">here</a></p></div> </div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Achievements
Team:Ciencias-UNAM/Achievements
2013-09-28T02:49:43Z
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Achievements</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br> We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br><br />
The principal aim of our project was to collaborate as much as possible to the iGEM mission to develop science freely. The parts we designed followed this principle, by being useful in very aspects, apart of our own project. We developed a Multiple Antibiotic Resistance protein, which acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1. This part can be used in several ways in different iGEM projects. We also sent to the parts registry the LL-37 peptide, with adequate codon usage, an improvement on part BBa_K875000, that allows LL-37 expression. <br><br><br />
As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Achievements
Team:Ciencias-UNAM/Achievements
2013-09-28T02:47:01Z
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<p><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Do the biological materials used in your lab work pose any of the following risks?</span></p><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:italic;text-decoration:none;color:#666666;">Risks to the safety and health of team members or others working in the lab?</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br> We characterised LL-37 antimicrobial peptide from Team Trieste 2008. They used Lac promoter to express this peptide, hoping to inhibit cell growth. They did not have positive results, as the control experiment growth the same way as the one with LL-37 expression with IPTG. We approached this problem with two solutions. The first problem we saw in Trieste's characterisation was that Lac promoter has a very high basal level of transcription. Given pSB1C3 has a high copy number, it is transcribing a high amount of LL-37, still with no IPTG in the media. This could mean that there is selection pressure on E. coli to loose the plasmid, which could be one of the reasons the did not see changes between the control cells and the cells under IPTG induction. We addressed this problem by changing the promoter, constructing device BBa_K1230007. This device is under the pBad promoter, which works with arabinose. This promoter has a basal level of almost 0, so we are sure that it is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they used. By changing the codons to produce more peptide in E. coli, the peptide may not have sufficient time to fold properly, altering it's function. We solved this problem by using the normal codon usage of this peptide, allowing it to fold properly into it's final conformation.<br />
<br><br><br />
The principal aim of our project was to collaborate as much as possible to the iGEM mission to develop science freely. The parts we designed followed this principle, by being useful in very aspects, apart of our own project. We developed a Multiple Antibiotic Resistance protein, which acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1. This part can be used in several ways in different iGEM projects. We also sent to the parts registry the LL-37 peptide, with adequate codon usage, an improvement on part BBa_K875000, that allows LL-37 expression. <br><br><br />
As part of our collection, we also designed the device BBa_K1230009, which is the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction, with the Elowitz repressilator. This part will allow an easy way to produce proteins by Arabinose induction, improving the way characterization is made on iGEM.<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Interviews
Team:Ciencias-UNAM/HumanPractices/Interviews
2013-09-28T02:36:44Z
<p>Dheredia: </p>
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Interviews</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br><i>Radio Interview</i><br><br />
In order to spread and transmit information about Synthetic Biology, iGEM, and this year’s project we managed to obtain a radio interview in one of Mexico’s most important cultural radio stations, Radio Educación. Currently, Radio Educación is a decentralized body of the Ministry of Education (in its origins it was not), coordinated by the National Council for Culture and the Arts, whose substantive work is to develop and disseminate educational expressions, culture and art of Mexico through the radio. It is the first educational and cultural broadcasting in Mexico. It transmits in the entire Mexico republic, Central American and in south of the United States, making programs reachable to huge audiences. <br><br><br />
In the past nine decades, Radio Education has been an institution generating radio forefront of providing innovative proposals in radio programming and formats, as well as the promotion of cultural initiatives. <br><br><br />
See more at: <a href=" http://www.radioeducacion.edu.mx/ ">Radio Educación</a> <br><br><br />
With this interview, placed on the 19 year old news program “Su casa y otros viajes”, we were able to popularize and make this cientific knowledge accessible to the general public, that is, we made an effort to lead scientific knowledge to those interested in the understanding or learning of such knowledge. <br><br><br />
The interview is right here:<br><br />
<iframe width="100%" height="166" scrolling="no" frameborder="no" src="https://w.soundcloud.com/player/?url=http%3A%2F%2Fapi.soundcloud.com%2Ftracks%2F111631104"></iframe><br />
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2013-09-28T02:04:59Z
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Dheredia
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2013-09-28T02:02:48Z
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2013-09-28T00:56:22Z
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2013-09-28T00:48:06Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Home
Team:Ciencias-UNAM/stylesheets/specificstyle Home
2013-09-28T00:47:18Z
<p>Dheredia: </p>
<hr />
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margin: 0px;<br />
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background-repeat: repeat;<br />
text-align: center;<br />
}<br />
<br />
div {<br />
background-repeat: no-repeat;<br />
}<br />
<br />
.raw_image {<br />
width: 100%;<br />
height: 100%;<br />
}<br />
<br />
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display: none;<br />
}<br />
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position: relative;<br />
left: -160px;<br />
width: 940px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
text-align: left;<br />
}<br />
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}<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM
Team:Ciencias-UNAM
2013-09-28T00:39:24Z
<p>Dheredia: </p>
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<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">ACHIEVEMENTS</span></p></div><br />
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<div id="u54_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">This year's project is an <i>In vivo</i> production of an antimicrobial peptide regulated by <i>quorum sensing</i>. We designed a synthetic system in <i>E. coli</i> to express an antimicrobial peptide (Cathelicidin LL-37) <i>in vivo</i> only in the presence of a quorum sensing molecule (AI-2) and expel it with an efflux pump in order to act against targeted gastrointestinal pathogens.</span></p></div><br />
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<div id="u55_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">We are the team from Facultad de Ciencias, UNAM in Mexico City. Our team brings together students and teachers from all over the country, with various backgrounds and interests.</span></p></div><br />
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<div id="u56_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">We submitted several devices mainly to test and use the following parts:<br><b>Cathelicidin LL-37:</b> An antimicrobial peptide against bacterial infection.<br> <b>MarA:</b> A peptide responsible for an efflux pump in <i>E. coli</i> that is related to its resistance to cathelicidin and similar peptides.</span></p></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Journalism
Team:Ciencias-UNAM/HumanPractices/Journalism
2013-09-28T00:28:38Z
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Journalism</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br><i><b>Participation in the 1st Mexican Synthetic Biology Forum</b></i><br />
<br>We participated with a presentation of this year's project, its complications and the fun in it, on this new space that aims to consolidate and bring together the actors involved in the nascent field of Mexcian synthetic biology: the 1st Synthetic Biology Forum.<br />
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The iGEM team Ollin Mx from the Professional Interdisciplinary Biotechnology Unit of the IPN (UPIBI), along with some of their teachers, were the hosts of the 1st Forum of Synthetic Biology, held in August 8. <br> <br><br />
This Forum was a space where students and researchers (not only those specifically dedicated to the area) can talk about the potential Mexico has in the matter, and the tools we can generate that can contribute to other areas of biological sciences and applied disciplines. <br> <br><br />
The Forum featured Dr. Augustino Martinez (CINVESTAV Irapuato), the M. in B. José Antonio Alonso Pavón (Genomic Sciences Center), MBA Geronimo Villanueva Noguera (Mexican Association of Synthetic Biology), Dr. Pablo Padilla Longoria (UNAM), in addition to presentations by iGEM teams from different Mexican institutions. <br> <br><br />
More than 300 attendees, plus ten remote viewers streaming on YouTube, witnessed the presentations of some of the major axes of Synthetic Biology in Mexico. The event was attended by members of iGEM teams and others were also streaming from their respective cities. <br> <br><br />
Soon consistent themes began to emerge, among which were: a) the urgency of focusing on generating institutional projects, b) the need to continue the collaboration and dialogue, and finally, c) the duality basic research / applied research and whether priority should be given between the two or have a mixed approach. <br />
<br><br><i><b>Synthetic Biology Seminar</b></i><br />
<br>We participated on the Synthetic Biology Seminar of the School of Science, UNAM, where we exposed our project to both undergraduate students from Biology and Engineering and professors, and we were able to generate interesting discussions and analysis regarding Synthetic Biology, Molecular Biology and Genetic Engineering. <br />
<br><br><i><b>Formal Petition to Mexican Congress to review DNA import/export politics.</b> </i><br />
<br>We addressed a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress. <br><br><br />
In Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), is a federal agency of the government of Mexico, which is linked to the Department of Regulation and Health Promotion of the Ministry of Health. <br><br><br />
COFEPRIS regulates, among many other ambits, the health inspection process, use, maintenance, import, export and disposal of equipment for investigative purposes. However, this regulation normative has flaws, and both imports and export processes are difficult to convey and tedious, not to say annoying. <br><br><br />
In our experience the genetic material for research goes through a lot of instances before being available to universities, laboratories or scientific institutions. <br><br><br />
The problem is that in Mexico there is no legislation that allows sending DNA sequences (which are not toxic at all, nor represent any possible damage, and are not found in living organisms, and there is no way for them to enter to one without appropriate techniques in a laboratory) so that packages sent from abroad with the material needed to work on different project are held by customs, and reviewed by COFEPRIS in a process that never takes less than a month, which means a long delay in the projects. <br><br><br />
This problem is not isolated to this iGEM competition, is a problem that all laboratories in the country have when they attempt to receive such material for research. <br><br><br />
This is why we addressed a letter explaining this situation to the Congressman named above, noting the urge for creating a law initiative in this matter, hoping to get a positive response that can culminate in the creation of a formal proposal to the Mexican Congress. <br />
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Journalism</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br><i>Participation in the 1st Mexican Synthetic Biology Forum</i><br />
<br>We participated with a presentation of this year's project, its complications and the fun in it, on this new space that aims to consolidate and bring together the actors involved in the nascent field of Mexcian synthetic biology: the 1st Synthetic Biology Forum.<br />
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The iGEM team Ollin Mx from the Professional Interdisciplinary Biotechnology Unit of the IPN (UPIBI), along with some of their teachers, were the hosts of the 1st Forum of Synthetic Biology, held in August 8. <br> <br><br />
This Forum was a space where students and researchers (not only those specifically dedicated to the area) can talk about the potential Mexico has in the matter, and the tools we can generate that can contribute to other areas of biological sciences and applied disciplines. <br> <br><br />
The Forum featured Dr. Augustino Martinez (CINVESTAV Irapuato), the M. in B. José Antonio Alonso Pavón (Genomic Sciences Center), MBA Geronimo Villanueva Noguera (Mexican Association of Synthetic Biology), Dr. Pablo Padilla Longoria (UNAM), in addition to presentations by iGEM teams from different Mexican institutions. <br> <br><br />
More than 300 attendees, plus ten remote viewers streaming on YouTube, witnessed the presentations of some of the major axes of Synthetic Biology in Mexico. The event was attended by members of iGEM teams and others were also streaming from their respective cities. <br> <br><br />
Soon consistent themes began to emerge, among which were: a) the urgency of focusing on generating institutional projects, b) the need to continue the collaboration and dialogue, and finally, c) the duality basic research / applied research and whether priority should be given between the two or have a mixed approach. <br />
<br><br><i>Synthetic Biology Seminar</i><br />
<br>We participated on the Synthetic Biology Seminar of the School of Science, UNAM, where we exposed our project to both undergraduate students from Biology and Engineering and professors, and we were able to generate interesting discussions and analysis regarding Synthetic Biology, Molecular Biology and Genetic Engineering. <br />
<br><br><i>Formal Petition to Mexican Congress to review DNA import/export politics. </i><br />
<br>We addressed a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress. <br><br><br />
In Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), is a federal agency of the government of Mexico, which is linked to the Department of Regulation and Health Promotion of the Ministry of Health. <br><br><br />
COFEPRIS regulates, among many other ambits, the health inspection process, use, maintenance, import, export and disposal of equipment for investigative purposes. However, this regulation normative has flaws, and both imports and export processes are difficult to convey and tedious, not to say annoying. <br><br><br />
In our experience the genetic material for research goes through a lot of instances before being available to universities, laboratories or scientific institutions. <br><br><br />
The problem is that in Mexico there is no legislation that allows sending DNA sequences (which are not toxic at all, nor represent any possible damage, and are not found in living organisms, and there is no way for them to enter to one without appropriate techniques in a laboratory) so that packages sent from abroad with the material needed to work on different project are held by customs, and reviewed by COFEPRIS in a process that never takes less than a month, which means a long delay in the projects. <br><br><br />
This problem is not isolated to this iGEM competition, is a problem that all laboratories in the country have when they attempt to receive such material for research. <br><br><br />
This is why we addressed a letter explaining this situation to the Congressman named above, noting the urge for creating a law initiative in this matter, hoping to get a positive response that can culminate in the creation of a formal proposal to the Mexican Congress. <br />
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<br>We participated with a presentation of this year's project, its complications and the fun in it, on this new space that aims to consolidate and bring together the actors involved in the nascent field of Mexcian synthetic biology: the 1st Synthetic Biology Forum.<br />
<br> <br><br />
The iGEM team Ollin Mx from the Professional Interdisciplinary Biotechnology Unit of the IPN (UPIBI), along with some of their teachers, were the hosts of the 1st Forum of Synthetic Biology, held in August 8. <br> <br><br />
This Forum was a space where students and researchers (not only those specifically dedicated to the area) can talk about the potential Mexico has in the matter, and the tools we can generate that can contribute to other areas of biological sciences and applied disciplines. <br> <br><br />
The Forum featured Dr. Augustino Martinez (CINVESTAV Irapuato), the M. in B. José Antonio Alonso Pavón (Genomic Sciences Center), MBA Geronimo Villanueva Noguera (Mexican Association of Synthetic Biology), Dr. Pablo Padilla Longoria (UNAM), in addition to presentations by iGEM teams from different Mexican institutions. <br> <br><br />
More than 300 attendees, plus ten remote viewers streaming on YouTube, witnessed the presentations of some of the major axes of Synthetic Biology in Mexico. The event was attended by members of iGEM teams and others were also streaming from their respective cities. <br> <br><br />
Soon consistent themes began to emerge, among which were: a) the urgency of focusing on generating institutional projects, b) the need to continue the collaboration and dialogue, and finally, c) the duality basic research / applied research and whether priority should be given between the two or have a mixed approach. <br />
<br><br><i>Synthetic Biology Seminar</i><br />
<br>We participated on the Synthetic Biology Seminar of the School of Science, UNAM, where we exposed our project to both undergraduate students from Biology and Engineering and professors, and we were able to generate interesting discussions and analysis regarding Synthetic Biology, Molecular Biology and Genetic Engineering. <br />
<br><br><i>Formal Petition to Mexican Congress to review DNA import/export politics. </i><br />
We addressed a letter to Congressman Rúben Benjamín Félix Hays, President of the Science and Technology Commission of the Mexican Congress. <br><br><br />
In Mexico the Federal Commission for Protection against Health Risks (COFEPRIS), is a federal agency of the government of Mexico, which is linked to the Department of Regulation and Health Promotion of the Ministry of Health. <br><br><br />
COFEPRIS regulates, among many other ambits, the health inspection process, use, maintenance, import, export and disposal of equipment for investigative purposes. However, this regulation normative has flaws, and both imports and export processes are difficult to convey and tedious, not to say annoying. <br><br><br />
In our experience the genetic material for research goes through a lot of instances before being available to universities, laboratories or scientific institutions. <br><br><br />
The problem is that in Mexico there is no legislation that allows sending DNA sequences (which are not toxic at all, nor represent any possible damage, and are not found in living organisms, and there is no way for them to enter to one without appropriate techniques in a laboratory) so that packages sent from abroad with the material needed to work on different project are held by customs, and reviewed by COFEPRIS in a process that never takes less than a month, which means a long delay in the projects. <br><br><br />
This problem is not isolated to this iGEM competition, is a problem that all laboratories in the country have when they attempt to receive such material for research. <br><br><br />
This is why we addressed a letter explaining this situation to the Congressman named above, noting the urge for creating a law initiative in this matter, hoping to get a positive response that can culminate in the creation of a formal proposal to the Mexican Congress. <br />
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Dheredia
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Team:Ciencias-UNAM/Project/WetLab/BioBricks
2013-09-28T00:15:40Z
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Results</span></p></div><br />
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<div id="u54_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Notebook</span></p></div><br />
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<div id="u56_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Modelling</span></p></div><br />
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<div id="u59_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">References</span></p></div><br />
</div><div id="u60" class="u60" ><br />
<DIV id="u60_line" class="u60_line" ></DIV><br />
</div><br />
<div id="u61" class="u61" ><br />
<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">BioBricks</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><br />
<br><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br />
<table style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;" border="1"><br />
<tr><br />
<th align="center">Name</th><br />
<th align="center">Type</th><br />
<th align="center">Description</th><br />
<th align="center">Designer</th><br />
<th align="center">Length</th><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230000" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230000</a></td><br />
<td align="center">&nbsp;Coding</td><br />
<td align="center">AcrAB-TolC multidrug efflux transport system activator from E. coli</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">393</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230001" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230001</a></td><br />
<td align="center">Protein_Domain</td><br />
<td align="center">LL-37 antimicrobial peptide</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">120</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230002" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230002</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">YFP generator with LsrA promoter, with terminator as a prefix</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1035</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230003" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230003</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">RBS-GFP-Terminator with LL-37 antimicrobial peptide with modified codon usage for E. coli as prefix</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1009</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230004" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230004</a></td><br />
<td align="center">Generator</td><br />
<td align="center">MarA Generator</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">619</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230007" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230007</a></td><br />
<td align="center">Generator</td><br />
<td align="center">araC-pBad-rbs-LL37</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1358</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230008" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230008</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">rbs-GFP-Terminator- pLsrA</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">988</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230009" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230009</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">AraC-pBad-rbs</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1229</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230010" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230010</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">araC-pBad-rbs-LL37-rbs-GFP</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">2244</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230011" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230011</a></td><br />
<td align="center">Generator</td><br />
<td align="center">araC-pBad-rbs-LL37</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1355</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230012" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230012</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">rbs-GFP-terminator-pLsrA-LL37</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1114</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230013" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230013</a></td><br />
<td align="center">Project</td><br />
<td align="center">araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">2871</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230014" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230014</a></td><br />
<td align="center">Intermediate</td><br />
<td align="center">rbs-GFP-pLacI-rbs-MarA Generator</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1505</td><br />
</tr><br />
<tr><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230015" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230015</a></td><br />
<td align="center">Project</td><br />
<td align="center">araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">2868</td><br />
</tr><br />
<tr class="tg-even"><br />
<td align="center"><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1230016" style="color: rgb(0, 43, 184); background-image: none; font-family: Arial, Helvitica, sans-serif; font-size: 11px; line-height: 12px; text-align: left; ">BBa_K1230016</a></td><br />
<td align="center">Project</td><br />
<td align="center">pLacI-rbs-MarA-rbs-GFP-double terminator-pLsrA-rbs-LL-37</td><br />
<td align="center">Andrés Ferriño Iriarte</td><br />
<td align="center">1741</td><br />
</tr><br />
</table><br />
<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> </span></p></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_BB
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2013-09-28T00:14:08Z
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a:hover {color:#667777;} <br />
a:active {color:#3e243a;} <br />
<br />
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position:absolute;<br />
left:190px;<br />
top:203px;<br />
width:272px;<br />
height:17px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:240px;<br />
width:150px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:263px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:282px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:301px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u51 {<br />
position:absolute;<br />
left:190px;<br />
top:317px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u52 {<br />
position:absolute;<br />
left:190px;<br />
top:332px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u53 {<br />
position:absolute;<br />
left:190px;<br />
top:348px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:365px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u55 {<br />
position:absolute;<br />
left:190px;<br />
top:383px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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position:absolute;<br />
left:190px;<br />
top:399px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u57 {<br />
position:absolute;<br />
left:190px;<br />
top:417px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u58 {<br />
position:absolute;<br />
left:190px;<br />
top:433px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u59 {<br />
position:absolute;<br />
left:190px;<br />
top:453px;<br />
width:150px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u60_line {<br />
background-image: url('https://static.igem.org/mediawiki/2013/e/e3/U73_line.PNG');<br />
}<br />
<br />
.u60_line {<br />
position:absolute;<br />
left:4px;<br />
top:0px;<br />
width:3px;<br />
height:223px;<br />
}<br />
<br />
.u60 {<br />
position:absolute;<br />
left:350px;<br />
top:245px;<br />
width:10px;<br />
height:223px;<br />
<br />
}<br />
<br />
.u61 {<br />
position:absolute;<br />
left:380px;<br />
top:240px;<br />
width:690px;<br />
height:143px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}</div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript_Home
Team:Ciencias-UNAM/scripts/pagescript Home
2013-09-28T00:12:30Z
<p>Dheredia: </p>
<hr />
<div>for(var i = 0; i < 85; i++) { var scriptId = 'u' + i; window[scriptId] = document.getElementById(scriptId); }<br />
$axure.eventManager.pageLoad( function (e) {<br />
if (true) {<br />
window.alert("a");<br />
SetPanelState('u77', 'pd1u77','swing','left',1000,'swing','left',1000);<br />
}<br />
});<br />
widgetIdToPanelStateChangeFunction['u77'] = function() { var e = windowEvent;<br />
if ((GetPanelState('u77')) == ('pd0u77')) { function waitued25c8b9db9d48d2b3a8c956cc4a03561() {<br />
window.alert("e");<br />
SetPanelState('u77', 'pd1u77','swing','left',500,'swing','left',500); } setTimeout(waitued25c8b9db9d48d2b3a8c956cc4a03561, 4000);<br />
} else if ((GetPanelState('u77')) == ('pd1u77')) { function waitu246a61a133734b3eaaad6bc8258475551() {<br />
window.alert("i");<br />
SetPanelState('u77', 'pd2u77','swing','left',500,'swing','left',500); } setTimeout(waitu246a61a133734b3eaaad6bc8258475551, 4000);<br />
} else if ((GetPanelState('u77')) == ('pd2u77')) { function waitub842a36bde6e473090f6e07be327e4371() {<br />
window.alert("o");<br />
SetPanelState('u77', 'pd0u77','swing','left',500,'swing','left',500); } setTimeout(waitub842a36bde6e473090f6e07be327e4371, 4000);<br />
}<br />
<br />
}<br />
gv_vAlignTable['u81'] = 'center';gv_vAlignTable['u83'] = 'center';HookHover('u10', false);<br />
gv_vAlignTable['u11'] = 'center';document.getElementById('u12_img').tabIndex = 0;<br />
HookHover('u12', false);<br />
<br />
u12.style.cursor = 'pointer';<br />
$axure.eventManager.click('u12', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Safety');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u13'] = 'center';document.getElementById('u16_img').tabIndex = 0;<br />
HookHover('u16', false);<br />
<br />
u16.style.cursor = 'pointer';<br />
$axure.eventManager.click('u16', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u17'] = 'center';document.getElementById('u18_img').tabIndex = 0;<br />
HookHover('u18', false);<br />
<br />
u18.style.cursor = 'pointer';<br />
$axure.eventManager.click('u18', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/GeneralDesign');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u19'] = 'center';document.getElementById('u20_img').tabIndex = 0;<br />
HookHover('u20', false);<br />
<br />
u20.style.cursor = 'pointer';<br />
$axure.eventManager.click('u20', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u21'] = 'center';document.getElementById('u22_img').tabIndex = 0;<br />
HookHover('u22', false);<br />
<br />
u22.style.cursor = 'pointer';<br />
$axure.eventManager.click('u22', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u23'] = 'center';document.getElementById('u24_img').tabIndex = 0;<br />
HookHover('u24', false);<br />
<br />
u24.style.cursor = 'pointer';<br />
$axure.eventManager.click('u24', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u25'] = 'center';document.getElementById('u26_img').tabIndex = 0;<br />
HookHover('u26', false);<br />
<br />
u26.style.cursor = 'pointer';<br />
$axure.eventManager.click('u26', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/References');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u27'] = 'center';document.getElementById('u30_img').tabIndex = 0;<br />
HookHover('u30', false);<br />
<br />
u30.style.cursor = 'pointer';<br />
$axure.eventManager.click('u30', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u31'] = 'center';document.getElementById('u32_img').tabIndex = 0;<br />
HookHover('u32', false);<br />
<br />
u32.style.cursor = 'pointer';<br />
$axure.eventManager.click('u32', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team/Sponsors');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u33'] = 'center';document.getElementById('u36_img').tabIndex = 0;<br />
HookHover('u36', false);<br />
<br />
u36.style.cursor = 'pointer';<br />
$axure.eventManager.click('u36', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u37'] = 'center';document.getElementById('u38_img').tabIndex = 0;<br />
HookHover('u38', false);<br />
<br />
u38.style.cursor = 'pointer';<br />
$axure.eventManager.click('u38', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Journalism');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u39'] = 'center';document.getElementById('u40_img').tabIndex = 0;<br />
HookHover('u40', false);<br />
<br />
u40.style.cursor = 'pointer';<br />
$axure.eventManager.click('u40', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Interviews');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u41'] = 'center';gv_vAlignTable['u43'] = 'center';u44.tabIndex = 0;<br />
<br />
u44.style.cursor = 'pointer';<br />
$axure.eventManager.click('u44', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href='https://2013.igem.org/Main_Page';<br />
<br />
}<br />
});<br />
gv_vAlignTable['u47'] = 'top';gv_vAlignTable['u49'] = 'top';gv_vAlignTable['u1'] = 'center';document.getElementById('u4_img').tabIndex = 0;<br />
HookHover('u4', false);<br />
<br />
u4.style.cursor = 'pointer';<br />
$axure.eventManager.click('u4', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u5'] = 'center';HookHover('u6', false);<br />
gv_vAlignTable['u7'] = 'center';HookHover('u8', false);<br />
gv_vAlignTable['u9'] = 'center';gv_vAlignTable['u51'] = 'top';gv_vAlignTable['u53'] = 'top';gv_vAlignTable['u54'] = 'top';gv_vAlignTable['u55'] = 'top';gv_vAlignTable['u56'] = 'top';gv_vAlignTable['u57'] = 'top';u58.tabIndex = 0;<br />
<br />
u58.style.cursor = 'pointer';<br />
$axure.eventManager.click('u58', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u58'] = 'top';u59.tabIndex = 0;<br />
<br />
u59.style.cursor = 'pointer';<br />
$axure.eventManager.click('u59', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u59'] = 'top';u60.tabIndex = 0;<br />
<br />
u60.style.cursor = 'pointer';<br />
$axure.eventManager.click('u60', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/BioBricks');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u60'] = 'top';u61.tabIndex = 0;<br />
<br />
u61.style.cursor = 'pointer';<br />
$axure.eventManager.click('u61', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/Achievements');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u61'] = 'top';gv_vAlignTable['u63'] = 'center';gv_vAlignTable['u65'] = 'center';gv_vAlignTable['u67'] = 'center';gv_vAlignTable['u69'] = 'center';gv_vAlignTable['u71'] = 'center';gv_vAlignTable['u73'] = 'center';gv_vAlignTable['u75'] = 'top';gv_vAlignTable['u76'] = 'top';gv_vAlignTable['u79'] = 'center';</div>
Dheredia
http://2013.igem.org/File:U82_normal.png
File:U82 normal.png
2013-09-27T22:01:09Z
<p>Dheredia: uploaded a new version of &quot;File:U82 normal.png&quot;</p>
<hr />
<div></div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript_Home
Team:Ciencias-UNAM/scripts/pagescript Home
2013-09-27T21:21:03Z
<p>Dheredia: </p>
<hr />
<div>for(var i = 0; i < 85; i++) { var scriptId = 'u' + i; window[scriptId] = document.getElementById(scriptId); }<br />
$axure.eventManager.pageLoad( function (e) {<br />
if (true) {<br />
window.alert("a");<br />
SetPanelState('u77', 'pd1u77','swing','left',1000,'swing','left',1000);<br />
}<br />
});<br />
widgetIdToPanelStateChangeFunction['u77'] = function() { var e = windowEvent;<br />
if ((GetPanelState('u77')) == ('pd0u77')) { function waitued25c8b9db9d48d2b3a8c956cc4a03561() {<br />
window.alert("e");<br />
SetPanelState('u77', 'pd1u77','swing','left',500,'swing','left',500); } setTimeout(waitued25c8b9db9d48d2b3a8c956cc4a03561, 4000);<br />
} else if ((GetPanelState('u77')) == ('pd1u77')) { function waitu246a61a133734b3eaaad6bc8258475551() {<br />
window.alert("i");<br />
SetPanelState('u77', 'pd2u77','swing','left',500,'swing','left',500); } setTimeout(waitu246a61a133734b3eaaad6bc8258475551, 4000);<br />
} else if ((GetPanelState('u77')) == ('pd2u77')) { function waitub842a36bde6e473090f6e07be327e4371() {<br />
window.alert("o");<br />
SetPanelState('u77', 'pd0u77','swing','left',500,'swing','left',500); } setTimeout(waitub842a36bde6e473090f6e07be327e4371, 4000);<br />
}<br />
<br />
}<br />
gv_vAlignTable['u81'] = 'center';gv_vAlignTable['u83'] = 'center';HookHover('u10', false);<br />
gv_vAlignTable['u11'] = 'center';document.getElementById('u12_img').tabIndex = 0;<br />
HookHover('u12', false);<br />
<br />
u12.style.cursor = 'pointer';<br />
$axure.eventManager.click('u12', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Safety');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u13'] = 'center';document.getElementById('u16_img').tabIndex = 0;<br />
HookHover('u16', false);<br />
<br />
u16.style.cursor = 'pointer';<br />
$axure.eventManager.click('u16', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u17'] = 'center';document.getElementById('u18_img').tabIndex = 0;<br />
HookHover('u18', false);<br />
<br />
u18.style.cursor = 'pointer';<br />
$axure.eventManager.click('u18', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/GeneralDesign');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u19'] = 'center';document.getElementById('u20_img').tabIndex = 0;<br />
HookHover('u20', false);<br />
<br />
u20.style.cursor = 'pointer';<br />
$axure.eventManager.click('u20', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u21'] = 'center';document.getElementById('u22_img').tabIndex = 0;<br />
HookHover('u22', false);<br />
<br />
u22.style.cursor = 'pointer';<br />
$axure.eventManager.click('u22', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u23'] = 'center';document.getElementById('u24_img').tabIndex = 0;<br />
HookHover('u24', false);<br />
<br />
u24.style.cursor = 'pointer';<br />
$axure.eventManager.click('u24', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/DryLab');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u25'] = 'center';document.getElementById('u26_img').tabIndex = 0;<br />
HookHover('u26', false);<br />
<br />
u26.style.cursor = 'pointer';<br />
$axure.eventManager.click('u26', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/References');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u27'] = 'center';document.getElementById('u30_img').tabIndex = 0;<br />
HookHover('u30', false);<br />
<br />
u30.style.cursor = 'pointer';<br />
$axure.eventManager.click('u30', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u31'] = 'center';document.getElementById('u32_img').tabIndex = 0;<br />
HookHover('u32', false);<br />
<br />
u32.style.cursor = 'pointer';<br />
$axure.eventManager.click('u32', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team/Sponsors');<br />
<br />
}<br />
});<br />
gv_vAlignTable['u33'] = 'center';document.getElementById('u36_img').tabIndex = 0;<br />
HookHover('u36', false);<br />
<br />
u36.style.cursor = 'pointer';<br />
$axure.eventManager.click('u36', function(e) {<br />
<br />
if (true) {<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices');<br />
<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/Achievements
Team:Ciencias-UNAM/Achievements
2013-09-27T21:18:43Z
<p>Dheredia: Created page with "<html> <head> <title>Achievements</title> <meta http-equiv="content-type" content="text/html; charset=utf-8"/> <meta http-equiv="imagetoolbar" content="no"/> <meta name="app..."</p>
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<p><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Do the biological materials used in your lab work pose any of the following risks?</span></p><br />
<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:italic;text-decoration:none;color:#666666;">Risks to the safety and health of team members or others working in the lab?</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> No the lab is a regular educational and basic investigation laboratory.<br />
</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:italic;text-decoration:none;color:#666666;">Risks to the safety and health of the general public, if released by design or by accident?</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"> No, as all the strains are non-pathogenic if there was a release of them it would not cause any harm. Also the sequences used for the design are from other non-pathogenic strains and the product of them are not harmful separately or the construction itself.<br />
</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:italic;text-decoration:none;color:#666666;">Risks to the environment, if released by design or by accident?</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">The possible risk for the environment would be against pathogenic strains due our design. Nevertheless, considering the probabilities for our design to be expressed outside laboratory conditions, we do not think there would be any risk to the environment.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:italic;text-decoration:none;color:#666666;">Risks to security through malicious misuse by individuals, groups, or countries?<br />
</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">The device itself does not contain any harmful DNA sequence, and as our strains are non-pathogenic ones could not be used for malicious misuse.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p align="justify"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise?</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">As our device is meant against pathogenic strains if it would become an industrial Project. We should first analyze the possible risks for being used in humans</span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">What safety training have you received (or plan to receive in the future)? and under what biosafety provisions will / do you work? </span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Our formation about safety comes from working in laboratories with a BioSafety level number 1, if we may work in the future in a laboratory with a different BioSafety level then we should get trained. The UNAM School of Science follows the biosafety regulations from the UNAM and in particular the recommendations made by the Environmental Management Unit. A more detailed protocol can be found at <b>(www.biomedicas.unam.mx/_administracion/_unidades_apoyo_inst/ manual_bioseguridad.pdf)</b>. Mexican regulations about biosafety are summarized at <b>http://amexbio.org/links.html</b> According to WHO Biosafety manual our lab and our chassis is rated as Biosafety level 1.<br />
<br><br><br />
The part is the human cathelicidin LL-37. The part is the BBa_K1230001 ( http://parts.igem.org/Part:BBa_K1230001). The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against Helicobacter pylori infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as Salmonella typhimurium and Escherichia coli. <br />
We are using it because we want our synthetic bacteria to express the LL-37 antimicrobial peptide in presence of pathogenic bacteria that express quorum sensing molecule AI-2.<br />
<br><br><br />
There is no potential safety/health risk for the team members, institution staff or general public by using the part that codes for de LL-37 antimicrobial peptide because is a human gene that is express only when the inmmune system interacts with a pathogenic microbe like Helicobacter pylori.<br />
<br><br<br />
The part is NOT listed under the Australia Group guidelines. The safety level of the lab was the BSL1 as the the strains and the coding regions used are non pathogenic and non potentially phatogenical when transcribed by our chassis organism.<br />
</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p></div><br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/scripts/data_Achievements
Team:Ciencias-UNAM/scripts/data Achievements
2013-09-27T21:18:25Z
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/scripts/pagescript_Achievements
Team:Ciencias-UNAM/scripts/pagescript Achievements
2013-09-27T21:17:11Z
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self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/Conclusions');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u25'] = 'center';document.getElementById('u26_img').tabIndex = 0;<br />
HookHover('u26', false);<br />
<br />
<br />
u26.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u26', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/References');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u27'] = 'center';document.getElementById('u30_img').tabIndex = 0;<br />
HookHover('u30', false);<br />
<br />
<br />
u30.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u30', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u31'] = 'center';document.getElementById('u32_img').tabIndex = 0;<br />
HookHover('u32', false);<br />
<br />
<br />
u32.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u32', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Team/Sponsors');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u33'] = 'center';document.getElementById('u36_img').tabIndex = 0;<br />
HookHover('u36', false);<br />
<br />
<br />
u36.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u36', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u37'] = 'center';document.getElementById('u38_img').tabIndex = 0;<br />
HookHover('u38', false);<br />
<br />
<br />
u38.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u38', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Journalism');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u39'] = 'center';gv_vAlignTable['u1'] = 'center';document.getElementById('u4_img').tabIndex = 0;<br />
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<br />
<br />
u4.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u4', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u5'] = 'center';HookHover('u6', false);<br />
gv_vAlignTable['u7'] = 'center';HookHover('u8', false);<br />
gv_vAlignTable['u9'] = 'center';document.getElementById('u40_img').tabIndex = 0;<br />
HookHover('u40', false);<br />
<br />
<br />
u40.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u40', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Interviews');<br />
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<br />
<br />
}<br />
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gv_vAlignTable['u41'] = 'center';gv_vAlignTable['u43'] = 'center';u44.tabIndex = 0;<br />
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u44.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u44', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href='https://2013.igem.org/Main_Page';<br />
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<br />
}<br />
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});<br />
gv_vAlignTable['u46'] = 'top';gv_vAlignTable['u47'] = 'top';HookHover('u10', false);<br />
gv_vAlignTable['u11'] = 'center';document.getElementById('u12_img').tabIndex = 0;<br />
HookHover('u12', false);<br />
<br />
<br />
u12.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u12', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Achievements');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u13'] = 'center';document.getElementById('u16_img').tabIndex = 0;<br />
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<br />
<br />
u16.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u16', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project');<br />
<br />
<br />
<br />
}<br />
<br />
});<br />
gv_vAlignTable['u17'] = 'center';document.getElementById('u18_img').tabIndex = 0;<br />
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<br />
<br />
u18.style.cursor = 'pointer';<br />
<br />
$axure.eventManager.click('u18', function(e) {<br />
<br />
<br />
<br />
if (true) {<br />
<br />
<br />
<br />
self.location.href=$axure.globalVariableProvider.getLinkUrl('https://2013.igem.org/Team:Ciencias-UNAM/Project/GeneralDesign');<br />
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<br />
}<br />
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});<br />
gv_vAlignTable['u19'] = 'center';</div>
Dheredia
http://2013.igem.org/File:U51_Overview.png
File:U51 Overview.png
2013-09-27T20:13:02Z
<p>Dheredia: uploaded a new version of &quot;File:U51 Overview.png&quot;</p>
<hr />
<div></div>
Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/stylesheets/specificstyle_Project
Team:Ciencias-UNAM/stylesheets/specificstyle Project
2013-09-27T20:09:42Z
<p>Dheredia: </p>
<hr />
<div>body {<br />
margin: 0px;<br />
background-color: #FFFFFF;<br />
background-image: url('https://static.igem.org/mediawiki/2013/d/d1/Bg_ciencias.png');<br />
background-position: left top;<br />
background-repeat: repeat;<br />
text-align: center;<br />
}<br />
<br />
div {<br />
background-repeat: no-repeat;<br />
}<br />
<br />
<br />
.raw_image {<br />
width: 100%;<br />
height: 100%;<br />
}<br />
<br />
.preload {<br />
display: none;<br />
}<br />
#main_container {<br />
position: relative;<br />
left: -160px;<br />
width: 940px;<br />
margin-left: auto;<br />
margin-right: auto;<br />
text-align: left;<br />
}<br />
<br />
.form_sketch {<br />
border-color: transparent;<br />
background-color: transparent;<br />
}<br />
<br />
<br />
.u0_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/a/ab/U0_normal_overview.png');<br />
height:800px;<br />
}<br />
<br />
.u0_container {<br />
position:absolute;<br />
left:160px;<br />
top:190px;<br />
width:940px;<br />
height:8000px;<br />
}<br />
<br />
#u0_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:946px;<br />
height:8050px;<br />
}<br />
<br />
.u1 {<br />
position:absolute;<br />
left:2px;<br />
top:717px;<br />
width:936px;<br />
height:16px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u2_menu {<br />
background-image: url('https://static.igem.org/mediawiki/2013/c/c3/U2_menu.png');<br />
}<br />
<br />
.u2_menu {<br />
position:absolute;<br />
left:157px;<br />
top:127px;<br />
width:949px;<br />
height:69px;<br />
}<br />
<br />
#u2_menu {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:946px;<br />
height:66px;<br />
}<br />
<br />
#u3 {<br />
position:absolute;<br />
left:2px;<br />
top:2px;<br />
width:941px;<br />
height:61px<br />
}<br />
<br />
.u4_mouseOver {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
.u4_selected {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
.u4_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/9/96/U4_normal.png');<br />
}<br />
<br />
.u4_container {<br />
position:absolute;<br />
left:0px;<br />
top:0px;<br />
width:150px;<br />
height:56px;<br />
}<br />
<br />
#u4_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:156px;<br />
height:62px;<br />
}<br />
<br />
.u5 {<br />
position:absolute;<br />
left:2px;<br />
top:18px;<br />
width:146px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u6_mouseOver {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
.u6_selected {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
.u6_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/9/96/U4_normal.png');<br />
}<br />
<br />
.u6_container {<br />
position:absolute;<br />
left:150px;<br />
top:0px;<br />
width:150px;<br />
height:56px;<br />
}<br />
<br />
#u6_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:156px;<br />
height:62px;<br />
}<br />
<br />
.u7 {<br />
position:absolute;<br />
left:2px;<br />
top:18px;<br />
width:146px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/84/U8_mouseOver.png');<br />
}<br />
<br />
.u8_selected {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/84/U8_mouseOver.png');<br />
}<br />
<br />
.u8_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/6/6f/U8_normal.png');<br />
}<br />
<br />
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position:absolute;<br />
left:300px;<br />
top:0px;<br />
width:250px;<br />
height:56px;<br />
}<br />
<br />
#u8_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:256px;<br />
height:62px;<br />
}<br />
<br />
.u9 {<br />
position:absolute;<br />
left:2px;<br />
top:18px;<br />
width:246px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/88/U4_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/9/96/U4_normal.png');<br />
}<br />
<br />
.u10_container {<br />
position:absolute;<br />
left:550px;<br />
top:0px;<br />
width:150px;<br />
height:56px;<br />
}<br />
<br />
#u10_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:156px;<br />
height:62px;<br />
}<br />
<br />
.u11 {<br />
position:absolute;<br />
left:2px;<br />
top:18px;<br />
width:146px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/c/ca/U12_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/c/ca/U12_mouseOver.png');<br />
}<br />
<br />
.u12_normal {<br />
background-image: url('https://static.igem.org/mediawiki/2013/e/e3/U12_normal.png');<br />
}<br />
<br />
.u12_container {<br />
position:absolute;<br />
left:700px;<br />
top:0px;<br />
width:236px;<br />
height:56px;<br />
}<br />
<br />
#u12_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:242px;<br />
height:62px;<br />
}<br />
<br />
.u13 {<br />
position:absolute;<br />
left:2px;<br />
top:18px;<br />
width:232px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/0/0a/U14_menu.png');<br />
}<br />
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position:absolute;<br />
left:149px;<br />
top:55px;<br />
width:159px;<br />
height:193px;<br />
}<br />
<br />
#u14_menu {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:156px;<br />
height:190px;<br />
}<br />
<br />
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position:absolute;<br />
left:2px;<br />
top:2px;<br />
width:151px;<br />
height:185px<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
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left:0px;<br />
top:0px;<br />
width:146px;<br />
height:30px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:152px;<br />
height:36px;<br />
}<br />
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position:absolute;<br />
left:2px;<br />
top:6px;<br />
width:142px;<br />
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;<br />
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;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
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left:0px;<br />
top:30px;<br />
width:146px;<br />
height:30px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:152px;<br />
height:36px;<br />
}<br />
<br />
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left:2px;<br />
top:6px;<br />
width:142px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('');<br />
}<br />
<br />
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background-image: url('');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
.u20_container {<br />
position:absolute;<br />
left:0px;<br />
top:60px;<br />
width:146px;<br />
height:30px;<br />
}<br />
<br />
#u20_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:152px;<br />
height:36px;<br />
}<br />
<br />
.u21 {<br />
position:absolute;<br />
left:2px;<br />
top:6px;<br />
width:142px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
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position:absolute;<br />
left:0px;<br />
top:90px;<br />
width:146px;<br />
height:30px;<br />
}<br />
<br />
#u22_img {<br />
position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:152px;<br />
height:36px;<br />
}<br />
<br />
.u23 {<br />
position:absolute;<br />
left:2px;<br />
top:6px;<br />
width:142px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
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left:0px;<br />
top:120px;<br />
width:146px;<br />
height:30px;<br />
}<br />
<br />
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position:absolute;<br />
left:-3px;<br />
top:-3px;<br />
width:152px;<br />
height:36px;<br />
}<br />
<br />
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position:absolute;<br />
left:2px;<br />
top:6px;<br />
width:142px;<br />
height:19px;<br />
;<br />
;<br />
;<br />
font-family:Arial;<br />
text-align:left;<br />
word-wrap:break-word;<br />
}<br />
<br />
.u26_mouseOver {<br />
background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
<br />
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background-image: url('https://static.igem.org/mediawiki/2013/8/86/U16_mouseOver.png');<br />
}<br />
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background-image: url('https://static.igem.org/mediawiki/2013/2/2d/U16_normal.png');<br />
}<br />
<br />
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left:0px;<br />
top:150px;<br />
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Dheredia
http://2013.igem.org/Team:Ciencias-UNAM/HumanPractices/Journalism
Team:Ciencias-UNAM/HumanPractices/Journalism
2013-09-27T20:04:04Z
<p>Dheredia: </p>
<hr />
<div><html><br />
<head><br />
<title>Journalism</title><br />
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<div id="u1_rtf"></div><br />
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<div id="u5_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HOME</span></p></div><br />
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Journalism</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br><i>Participation in the 1st Mexican Synthetic Biology Forum</i><br />
<br>We participated with a presentation of this year's project, its complications and the fun in it, on this new space that aims to consolidate and bring together the actors involved in the nascent field of Mexcian synthetic biology: the 1st Synthetic Biology Forum.<br />
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The iGEM team Ollin Mx from the Professional Interdisciplinary Biotechnology Unit of the IPN (UPIBI), along with some of their teachers, were the hosts of the 1st Forum of Synthetic Biology, held in August 8. <br> <br><br />
This Forum was a space where students and researchers (not only those specifically dedicated to the area) can talk about the potential Mexico has in the matter, and the tools we can generate that can contribute to other areas of biological sciences and applied disciplines. <br> <br><br />
The Forum featured Dr. Augustino Martinez (CINVESTAV Irapuato), the M. in B. José Antonio Alonso Pavón (Genomic Sciences Center), MBA Geronimo Villanueva Noguera (Mexican Association of Synthetic Biology), Dr. Pablo Padilla Longoria (UNAM), in addition to presentations by iGEM teams from different Mexican institutions. <br> <br><br />
More than 300 attendees, plus ten remote viewers streaming on YouTube, witnessed the presentations of some of the major axes of Synthetic Biology in Mexico. The event was attended by members of iGEM teams and others were also streaming from their respective cities. <br> <br><br />
Soon consistent themes began to emerge, among which were: a) the urgency of focusing on generating institutional projects, b) the need to continue the collaboration and dialogue, and finally, c) the duality basic research / applied research and whether priority should be given between the two or have a mixed approach. <br />
<br><br><i>Synthetic Biology Seminar</i><br />
<br>We participated on the Synthetic Biology Seminar of the School of Science, UNAM, where we exposed our project to both undergraduate students from Biology and Engineering and professors, and we were able to generate interesting discussions and analysis regarding Synthetic Biology, Molecular Biology and Genetic Engineering. <br />
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<p>Dheredia: </p>
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:20px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Journalism</span><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"></span></p><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;"><br><i>Participation in the 1st Mexican Synthetic Biology Forum</i><br />
<br>We participated with a presentation of this year's project, its complications and the fun in it, on this new space that aims to consolidate and bring together the actors involved in the nascent field of Mexcian synthetic biology: the 1st Synthetic Biology Forum.<br />
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The iGEM team Ollin Mx from the Professional Interdisciplinary Biotechnology Unit of the IPN (UPIBI), along with some of their teachers, were the hosts of the 1st Forum of Synthetic Biology, held in August 8. <br> <br><br />
This Forum was a space where students and researchers (not only those specifically dedicated to the area) can talk about the potential Mexico has in the matter, and the tools we can generate that can contribute to other areas of biological sciences and applied disciplines. <br> <br><br />
The Forum featured Dr. Augustino Martinez (CINVESTAV Irapuato), the M. in B. José Antonio Alonso Pavón (Genomic Sciences Center), MBA Geronimo Villanueva Noguera (Mexican Association of Synthetic Biology), Dr. Pablo Padilla Longoria (UNAM), in addition to presentations by iGEM teams from different Mexican institutions. <br> <br><br />
More than 300 attendees, plus ten remote viewers streaming on YouTube, witnessed the presentations of some of the major axes of Synthetic Biology in Mexico. The event was attended by members of iGEM teams and others were also streaming from their respective cities. <br> <br><br />
Soon consistent themes began to emerge, among which were: a) the urgency of focusing on generating institutional projects, b) the need to continue the collaboration and dialogue, and finally, c) the duality basic research / applied research and whether priority should be given between the two or have a mixed approach. <br />
<br><br><i>Synthetic Biology Seminar</i><br />
We participated on the Synthetic Biology Seminar of the School of Science, UNAM, where we exposed our project to both undergraduate students from Biology and Engineering and professors, and we were able to generate interesting discussions and analysis regarding Synthetic Biology, Molecular Biology and Genetic Engineering. <br />
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