http://2013.igem.org/wiki/index.php?title=Special:Contributions/Santiago&feed=atom&limit=50&target=Santiago&year=&month=2013.igem.org - User contributions [en]2024-03-28T09:40:39ZFrom 2013.igem.orgMediaWiki 1.16.5http://2013.igem.org/World_Championship_Jamboree/Practice_SessionsWorld Championship Jamboree/Practice Sessions2013-10-31T10:20:11Z<p>Santiago: </p>
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<p class="contentheader">Practice Sessions - November 01</p><br />
<p>Use this sign-up sheet to sign up for a practice session slot on Friday night (<b>November 1st</b>) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. Be sure to bring necessary computer equipment with you, such as chargers and adapters, as these will not be provided.</p><br />
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<p>There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room (please see the <a href="https://2013.igem.org/World_Championship_Jamboree/Map">campus map</a> for building locations). This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.</p><br />
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<p>Also, pre-registration will be available on Friday November 1st, starting at 3pm at <b>Building 12, Room 156</b>. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2013.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>).</p> <br />
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<p><strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Additional rooms may be added in the coming weeks.<br />
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<th style="width:100px;">Time</th><br />
<th>RM 32-123 </th><br />
<th>RM 32-141 </th><br />
<th>RM 32-155 </th><br />
<th>RM 34-101 </th><br />
<th>RM 56-114 </th><br />
<th>RM 56-154 </th><br />
<th>RM 66-144 </th><br />
<th>RM 66-168 </th><br />
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<th>5:00 - 5:30PM</th><br />
<td>BIT-China</td><br />
<td><i>unavailable</i></td><br />
<td>SYSU-Software</td><br />
<td>ZJU-China</td><br />
<td>Valencia Biocampus</td><br />
<td>UANL_Mty-Mexico</td><br />
<td>A7</td><br />
<td>A8</td><br />
</tr><br />
<tr class="even"><br />
<th>5:30 - 6:00PM</th><br />
<td>Freiburg</td><br />
<td><i>unavailable</i></td><br />
<td>TU-Eindhoven</td><br />
<td>Tokyo_Tech</td><br />
<td>Bielefeld-Germany</td><br />
<td>USTC_CHINA</td><br />
<td>UCL_E</td><br />
<td>B8</td><br />
</tr><br />
<tr class="even"><br />
<th>6:00 - 6:30PM</th><br />
<td>Dundee</td><br />
<td>UNITN-Trento</td><br />
<td>Göttingen</td><br />
<td>HokkaidoU_Japan</td><br />
<td>Heidelberg</td><br />
<td>GeorgiaTech</td><br />
<td>C7</td><br />
<td>C8</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30 - 7:00PM</th><br />
<td>SJTU-BioX-Shanghai</td><br />
<td>SydneyUni_Australia</td><br />
<td>HUST-China</td><br />
<td>NJU China</td><br />
<td>Newcastle</td><br />
<td>D6</td><br />
<td>D7</td><br />
<td>D8</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00 - 7:30PM</th><br />
<td>uOttawa</td><br />
<td>SYSU-China</td><br />
<td>XMU Software</td><br />
<td>Peking</td><br />
<td>Hong_Kong_HKUST</td><br />
<td>E6</td><br />
<td>E7</td><br />
<td>E8</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30 - 8:00PM</th><br />
<td>XMU China</td><br />
<td>Braunschweig</td><br />
<td>SUSTC-Shenzhen-B</td><br />
<td>NCTU_Formosa</td><br />
<td>Hong_Kong_CUHK</td><br />
<td>F6</td><br />
<td>WHU-China</td><br />
<td>F8</td><br />
</tr><br />
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<th>8:00 - 8:30PM</th><br />
<td>Calgary</td><br />
<td>UC Berkeley</td><br />
<td>USTC-Software</td><br />
<td>NYMU-Taipei</td><br />
<td>UC_Davis</td><br />
<td>UIUC Illinois</td><br />
<td>Fudan</td><br />
<td>Buenos_Aires</td><br />
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<tr class="even"><br />
<th>8:30 - 9:00PM</th><br />
<td>TU-Munich</td><br />
<td>ETH Zurich</td><br />
<td>Shenzhen_BGIC_0101</td><br />
<td>Virginia</td><br />
<td>Cornell</td><br />
<td>Tsinghua</td><br />
<td>EPF_Lausanne</td><br />
<td>Manchester</td><br />
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<th>9:00 - 9:30PM</th><br />
<td>Tianjin</td> <br />
<td>Imperial College</td><br />
<td>Shenzhen_BGIC_ATCG</td><br />
<td>Paris_Bettencourt</td><br />
<td>Penn</td><br />
<td>Yale</td><br />
<td>Calgary_Entrepreneurial</td><br />
<td>UCSF</td><br />
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</html></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T02:01:50Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|left|150 px|caption]] <div id="inwiki">'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
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{| class="wikitable" style="margin:auto; background: #FFF; border: 0px;"<br />
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<h1><img align="left" src="https://static.igem.org/mediawiki/2013/thumb/4/4e/Mapa_mundial_arsenico.jpg/500px-Mapa_mundial_arsenico.jpg" alt="Smiley face" height="400" width="600"></h1><br />
</figure> <br />
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<h1><img src="https://static.igem.org/mediawiki/2013/thumb/9/96/Colecta_de_muestras_de_agua.jpg/800px-Colecta_de_muestras_de_agua.jpg" alt="Smiley face" height="400" width="600"> </h1><br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:59:57Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|left|150 px|caption]] <div id="inwiki">'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
<br />
<br />
{| class="wikitable" style="margin:auto; background: #FFF; border: 0px;"<br />
|+ Arsenic destribution<br />
|-<br />
|<br />
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<div class="gallery autoplay items-2"><br />
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<div class="gallery items-2"><br />
<div id="item-1" class="control-operator"></div><br />
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<figure class="item"><br />
<h1><img align="left" src="https://static.igem.org/mediawiki/2013/thumb/4/4e/Mapa_mundial_arsenico.jpg/500px-Mapa_mundial_arsenico.jpg" alt="Smiley face" height="400" width="600"></h1><br />
</figure> <br />
<br />
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<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/9/96/Colecta_de_muestras_de_agua.jpg/800px-Colecta_de_muestras_de_agua.jpg" alt="Smiley face" height="400" width="600"> </h1><br />
</figure><br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:59:24Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|left|120 px|caption]] <div id="inwiki">'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
<br />
<br />
{| class="wikitable" style="margin:auto; background: #FFF; border: 0px;"<br />
|+ Arsenic destribution<br />
|-<br />
|<br />
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<figure class="item"><br />
<h1><img align="left" src="https://static.igem.org/mediawiki/2013/thumb/4/4e/Mapa_mundial_arsenico.jpg/500px-Mapa_mundial_arsenico.jpg" alt="Smiley face" height="400" width="600"></h1><br />
</figure> <br />
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<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/9/96/Colecta_de_muestras_de_agua.jpg/800px-Colecta_de_muestras_de_agua.jpg" alt="Smiley face" height="400" width="600"> </h1><br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:59:12Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|left|150 px|caption]] <div id="inwiki">'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
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{| class="wikitable" style="margin:auto; background: #FFF; border: 0px;"<br />
|+ Arsenic destribution<br />
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</figure> <br />
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<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/9/96/Colecta_de_muestras_de_agua.jpg/800px-Colecta_de_muestras_de_agua.jpg" alt="Smiley face" height="400" width="600"> </h1><br />
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<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|left|200 px|caption]] <div id="inwiki">'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:57:03Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|frameless|border|200 px]] <div id="inwiki"><br />
'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:54:50Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|200px|thumb|'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.]] <div id="inwiki"><br />
<br />
<br />
<br />
<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-28T01:53:34Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
[[File:Icono_proyecto.jpg|200px|thumb|caption]] <div id="inwiki"><br />
'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
<br />
<br />
<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain accurate information. Our central goal is helping to solve this situation, that is why we thought of a '''biosensor that was cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and correspondingly in ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on top of the list of countries with high natural arsenic concentration in certain zones ground water. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
<br />
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</div></div>Santiagohttp://2013.igem.org/File:Icono_proyecto.jpgFile:Icono proyecto.jpg2013-10-28T01:50:13Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_mapaarsenicoTeam:Buenos Aires/ mapaarsenico2013-10-28T01:34:44Z<p>Santiago: /* Testing water samples from different locations */</p>
<hr />
<div><div id="external"><br />
<br />
=Testing water samples from different locations=<br />
<br />
Our project wouldn’t be fullfilled if we didn’t bring it to the society. To accomplish that, we are trying to make a map indicating the level of arsenite pollution in each location. However, we must know that due to the strange distribution of arsenic in groundwater,the detection of arsenic in a sample doesn't mean that the whole location is contamianted.<br />
<br />
<br />
[[File:Colecta_de_muestras_de_agua.jpg|center|800px]]<br />
<br />
<br />
We have collected sample water from different towns around the country, specialy from the nearby of Buenos Aires, and we have measured the arsenic concentration of each sample.<br />
<br />
Please, click on any picture if you want to see the interactive map ( where you will be able to see the concentration of arsenic in each location)<br />
<br />
<br />
[[File:Mapa_grande.png|center|600px|link=https://mapsengine.google.com/map/edit?mid=zvrFtMv4aIbU.k9K3FAj1-88Y|]]<br />
<br />
<br />
[[File:Mapa_chico.png|center|600px|link=https://mapsengine.google.com/map/edit?mid=zvrFtMv4aIbU.k9K3FAj1-88Y|]]<br />
<br />
<br />
</div>.</div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_mapaarsenicoTeam:Buenos Aires/ mapaarsenico2013-10-28T01:33:47Z<p>Santiago: /* Testing water samples from different locations */</p>
<hr />
<div><div id="external"><br />
<br />
=Testing water samples from different locations=<br />
<br />
Our project wouldn’t be fullfilled if we didn’t bring it to the society. To accomplish that, we are trying to make a map indicating the level of arsenite pollution in each location. However, we must know that due to the strange distribution of arsenic in groundwater,the detection of arsenic in a sample doesn't mean that the whole location is contamianted.<br />
<br />
<br />
[[File:Colecta_de_muestras_de_agua.jpg|center|800px]]<br />
<br />
<br />
We have collected sample water from different towns around the country, specialy from the nearby of Buenos Aires.<br />
<br />
Please, click on any picture if you want to see the interactive map ( where you will be able to see the concentration of arsenic in each location)<br />
<br />
<br />
[[File:Mapa_grande.png|center|600px|link=https://mapsengine.google.com/map/edit?mid=zvrFtMv4aIbU.k9K3FAj1-88Y|]]<br />
<br />
<br />
[[File:Mapa_chico.png|center|600px|link=https://mapsengine.google.com/map/edit?mid=zvrFtMv4aIbU.k9K3FAj1-88Y|]]<br />
<br />
<br />
</div>.</div>Santiagohttp://2013.igem.org/File:Mapa_chico.pngFile:Mapa chico.png2013-10-28T01:33:09Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_mapaarsenicoTeam:Buenos Aires/ mapaarsenico2013-10-28T01:29:25Z<p>Santiago: /* Testing water samples from different locations */</p>
<hr />
<div><div id="external"><br />
<br />
=Testing water samples from different locations=<br />
<br />
Our project wouldn’t be fullfilled if we didn’t bring it to the society. To accomplish that, we are trying to make a map indicating the level of arsenite pollution in each location. However, we must know that due to the strange distribution of arsenic in groundwater,the detection of arsenic in a sample doesn't mean that the whole location is contamianted.<br />
<br />
<br />
[[File:Colecta_de_muestras_de_agua.jpg|center|800px]]<br />
<br />
<br />
We are collecting sample water from different towns in the nearby of Buenos Aires. In the near future, we are going to test this samples with our system.<br />
<br />
<br />
[[File:Mapa_grande.png|center|800px|link=https://mapsengine.google.com/map/edit?mid=zvrFtMv4aIbU.k9K3FAj1-88Y|]]<br />
<br />
<br />
</div>.</div>Santiagohttp://2013.igem.org/File:Mapa_grande.pngFile:Mapa grande.png2013-10-28T01:27:22Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T01:07:40Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [https://2013.igem.org/Team:Buenos_Aires#Human Practice human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_abstractTeam:Buenos Aires/ abstract2013-10-28T01:05:35Z<p>Santiago: /* Abstract */</p>
<hr />
<div><div id="external"><br />
<br />
=Abstract=<br />
<br />
Our project is focused on developing a biosensor for certain water pollutants, with a modular and scalable approach. This approach would make it easy to adapt the response for the detection of different substances.<br />
Until now, there have been some experiences of pollutant biosensing in iGEM, but most of them rely on expensive and specific equipment, or qualified people to interpret the results. Being aware that most of the populations affected by consumption of contaminated groundwater don’t have scientific or technical training, we intend the device to be cheap and easily distributed. We also have designed it in a way that any user with minimal training (using an image-based instructions) could easily determine the presence and level of the contaminant on drinking water. The project will focus on measuring a primary pollutant: arsenic. However, its modular and scalable design provides an easy way to measure various contaminants such as nitrate/nitrite, lead, hydrocarbons, etcetera. <br />
We are working with industrial designers and 3D printing technology (see [[attribution]]) to expand our initial idea to the industry production. We now have a prototype of the physical devise and are testing the hole biological design before assembling all together. We have also measured field samples with a simplified model of our hole system. <br />
We expect to have all assembled together and ready in the user hands in the near future. <br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T01:04:17Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [https://2013.igem.org/Team:Buenos_Aires#Human Practice | human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T01:03:45Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [#Human Practice| human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:59:53Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [[https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico | human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:58:56Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [[https://2013.igem.org/Team:Buenos_Aires#Human Practice | human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:57:31Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenic concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [https://2013.igem.org/Team:Buenos_Aires#Human Practice| human practice section ]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:54:36Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenci concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico| human practice section ]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:54:15Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenci concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [[https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico| human practice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:53:20Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
We then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenci concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
For more information about the sample locations and their arsenic concentrations, please visit our [[https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico|human parctice section ]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:50:56Z<p>Santiago: /* measure of groundwater samples with pars_mRFP */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
we then compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenci concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
for more information of the sample locations and arsenci concentrations, please visit our [[human parctice section | https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico]]<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:50:16Z<p>Santiago: /* mRFP under Arsenite Inducible Promoter (Bba_K1106003) characterization */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
==measure of groundwater samples with pars_mRFP==<br />
<br />
Using the experiment above as a calibration curve, we calculated the concentration of arsenite in groundwater samples. To achive that, we first had to linearize the curve. As the curve seems to fit a logaritmic function, we apliied a logaritmic transformation to the orginal data.<br />
<br />
[[File:Ajuste_lineal_RFP.png|center|600px|]]<br />
<br />
we the compared our method to an accurated method for arsenic detection (Atomic Absorption Spectrometry). the results show taht, despite pars_rfp contruction is not as accurate as AAS, it can be used as a method to indicate the arsenci concentration at different ranges (and that is what we actualy needed).<br />
<br />
[[File:Correlacion_arsenico.png|center|600px|]]<br />
<br />
for more information of the sample locations and arsenci concentrations, please visit our [[human parctice section https://2013.igem.org/Team:Buenos_Aires/_mapaarsenico]]<br />
<br />
<br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/File:Correlacion_arsenico.pngFile:Correlacion arsenico.png2013-10-28T00:49:48Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/File:Ajuste_lineal_RFP.pngFile:Ajuste lineal RFP.png2013-10-28T00:42:10Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_resqrfpTeam:Buenos Aires/ resqrfp2013-10-28T00:31:23Z<p>Santiago: /* mRFP production at different arsenite concentrations */</p>
<hr />
<div><div id="external"><br />
<br />
=mRFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) characterization=<br />
<br />
'''Objective'''<br />
<br />
Asess mRFP production, stability and naked eye discernibility range under inducible conditions.<br />
<br />
'''General procedure'''<br />
<br />
Different assays were performed using ''E. coli'' (DH5α strain) harbouring a plasmid that encodes mRFP under arsenite inducible promoter (ArsRFP culture).<br />
<br />
<br />
<br />
== '''mRFP production at different arsenite concentrations''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 25, 50, 100, 500 and 1000ppb). 1ml aliquots were taken after 24 hours and fluorescence was measured.<br />
<br />
'''Results'''<br />
<br />
mRFP fluorescence increases with higher arsenite concentrations, in a sigmoidal way.<br />
<br />
[[File:Exp_Ine.jpg|center|600px|]]<br />
<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP production over time''' ==<br />
<br />
<br />
'''Method'''<br />
<br />
A 100 ml ArsRFP culture was grown at 30°C until it reached OD=0.4 (OD 600nm). At this point arsenite was added (1000ppb final concentration) and fluorescence was measured every 30 minutes during 8 hours at 584 nm excitation peak and 608 nm emission peak. <br />
<br />
'''Results'''<br />
<br />
As shown in the figure below, mRFP production increses over time with arsenite (1000 ppb). However, there is a 3 hours lag after inoculation.<br />
<br />
[[File:RFP_induccion.jpg|center|600px|]]<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''mRFP stability over time''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP culture was grown overnight at 37ºC in 10 ml LB medium with 2000ppb arsenite concentration. The following day, the culture was centrifuged and the supernatant was discarded in order to remove the arsenite, thus stopping the induction. Afterwars, fresh LB medium was added and the pellet was resuspended. This was done twice and the culture was returned to 37ºC incubation. 1 ml of this culture was taken every 12 hours for the following 4 days. Finally, fluorescence was measured. <br />
<br />
'''Results'''<br />
<br />
mRFP degradation is shown over time, specially during the first 24 hours.<br />
<br />
[[File:RFP_santi.jpg|center|600px]]<br />
<br />
<br />
<html><br />
<br><br />
<br><br />
</html><br />
<br />
== '''Naked eye discernibility range of mRFP production by arsenite inducible promoter''' ==<br />
<br />
'''Method'''<br />
<br />
ArsRFP cultures were grown with different arsenite concentrations ( 0, 10, 50, 200 and 1000ppb). 1ml aliquots were taken every 12 hours and centrifuged at 10.000rpm for 5 minutes. Pellets pictures were taken in order to compare the range of colour at naked eye. mRFP fluorescence was also measured with a fluorimeter at 484nm for excitation and 608 nm for emission.<br />
<br />
'''Results'''<br />
<br />
As it can be seen in the pictures below, a difference in the colour production can be clearly distinguished between different arsenite concentrations after 24 hours of induction and between the higher arsenite concentrations only. It can also be observed that over time the production grows and that after 62 hours the difference between 200 and 1000ppb is not clear. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff;margin:auto;" <br />
|+Induction over time at different concentrations of arsenite (ppb)<br />
|-<br />
|After 12 hours of induction:<br />
[[File:T1rfp.jpg | center | 400px]]<br />
|-<br />
|After 24 hours of induction:<br />
[[File:T2rfp2.jpg| center | 400px]]<br />
|-<br />
|After 36 hours of induction:<br />
[[File:T3rfp.jpg | center | 400px]]<br />
|-<br />
|After 48 hours of induction:<br />
[[File:T4'rfp.jpg | center | 400px]]<br />
|-<br />
|After 50 hours of induction:<br />
[[File:T5rfp.jpg | center | 400px]]<br />
|-<br />
|After 62 hours of induction:<br />
[[File:T6rfp.jpg| center | 400px]]<br />
|}<br />
<br />
<br />
== '''Collaboration with iGEM Tec-Monterrey team''' ==<br />
<br />
We established a collaboration with the iGEM Tec-Monterrey team, and exchanged constructions to characterize. In this link you may find their characterization of our mRFP under pArs: [[https://2013.igem.org/Team:TecMonterrey/collab_argentina.html Tec-Monterrey characterization]].<br />
<br />
== '''Overall conclusions''' ==<br />
<br />
mRFP production responds efficiently under inducible conditions, both over time and different arsenite concentrations. The mRFP stability is acceptable for the aim of our Project. However, the visibility to the naked eye is not sufficient at low arsenite concentrations. <br />
We reached to the conclusion that, in order to increase the colour production at lower arsenite concentrations, a signal amplification system has to be added. It could also be added some switch to stop the production and avoid saturation of all the samples, and thus keep the range of colour observable to the naked eye.<br />
<br />
</div></div>Santiagohttp://2013.igem.org/File:Exp_Ine.jpgFile:Exp Ine.jpg2013-10-28T00:29:58Z<p>Santiago: uploaded a new version of &quot;File:Exp Ine.jpg&quot;</p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_outreachTeam:Buenos Aires/ outreach2013-10-27T23:14:09Z<p>Santiago: /* lecture in Instituto Oral Modelo for death students */</p>
<hr />
<div><div id="external"><br />
<br />
=Lecture in Saint Roman High School= <br />
On the morning of 5th August, members of our iGEM team gave a lecture at Saint Román High School. They introduced the basic knowledge of Synthetic Biology to the students and helped them comprehend how molecular biology works. Afterwards, they played a game in which the students had to think and design a genetic machine for a specific problem. They were curious about the biology world and asked many questions related to our work.<br />
<br />
<br />
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<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/0/05/Buenosaires2013hp2.JPG/600px-Buenosaires2013hp2.JPG" alt="Smiley face"></h1><br />
</figure><br />
<br />
<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/f/f0/Buenosaires2013hp3.JPG/600px-Buenosaires2013hp3.JPG" alt="Smiley face" ></h1><br />
</figure><br />
<br />
<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/d/db/Buenosaires2013hp5.JPG/600px-Buenosaires2013hp5.JPG" alt="Smiley face" ></h1><br />
</figure><br />
<br />
<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/7/7c/Buenosaires2013hp6.JPG/600px-Buenosaires2013hp6.JPG" alt="Smiley face"></h1><br />
</figure><br />
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<br />
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</html><br />
<br />
<br />
=Lecture in Instituto Oral Modelo for death students = <br />
On 30 th August, three members of our iGEM team went to Instituto Oral Modelo in order to explain what molecular biology and Synthetic biology are. we also did some experiments with them: one of them was to extract DNA from a banana using only shampoo, salt and alcohol.<br />
<br />
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<h1><img src="https://static.igem.org/mediawiki/2013/thumb/8/8b/BairesHP1.jpg/600px-BairesHP1.jpg" alt="Smiley face"></h1><br />
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<figure class="item"><br />
<h1><img src="https://static.igem.org/mediawiki/2013/thumb/a/a8/BairesHP2.jpg/600px-BairesHP2.jpg" alt="Smiley face" ></h1><br />
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<h1><img src="https://static.igem.org/mediawiki/2013/thumb/7/74/BairesHP13.jpg/600px-BairesHP13.jpg" alt="Smiley face"></h1><br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_outreachTeam:Buenos Aires/ outreach2013-10-27T23:13:53Z<p>Santiago: /* Introducing science to deaf students */</p>
<hr />
<div><div id="external"><br />
<br />
=Lecture in Saint Roman High School= <br />
On the morning of 5th August, members of our iGEM team gave a lecture at Saint Román High School. They introduced the basic knowledge of Synthetic Biology to the students and helped them comprehend how molecular biology works. Afterwards, they played a game in which the students had to think and design a genetic machine for a specific problem. They were curious about the biology world and asked many questions related to our work.<br />
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<br />
=lecture in Instituto Oral Modelo for death students = <br />
On 30 th August, three members of our iGEM team went to Instituto Oral Modelo in order to explain what molecular biology and Synthetic biology are. we also did some experiments with them: one of them was to extract DNA from a banana using only shampoo, salt and alcohol.<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_attributionTeam:Buenos Aires/ attribution2013-10-27T23:05:50Z<p>Santiago: /* Attributions */</p>
<hr />
<div><div id="external"><br />
<br />
=Attributions=<br />
<br />
==wet lab==<br />
the members of our team who had worked in the wet lab are:<br />
#Ines Patop<br />
#Sebastian Mildiner<br />
#Luciano Marasco<br />
#Lucas Vattino<br />
#Santiago sosa<br />
<br />
the advisors who had helped us in the wet lab are:<br />
#Nicolas Nieto Moreno<br />
#Alicia Grande<br />
#Hernan Bonomi<br />
#Nicolas Carlotto<br />
<br />
==modelling==<br />
#Federico Barone<br />
#Edgar Altsyler (who had helped federico with the modelling)<br />
<br />
==wiki design and multimedia work==<br />
<br />
#Francisco Dorr was the member in charge of the wiki<br />
<br />
# Some of the wiki pictures and images were made by Nicole Shrewsbury and Sebastian Vishnopolska. <br />
<br />
# Video, Photos and Audio were made by Gonzalo Moiguer, Rodrigo Melendez and Javier de Azkue.<br />
<br />
==Prototype Design==<br />
# The design of the physical device was performed by the industrial designers Romina Mathieu, Luciana Feo Mourelle and Adrián Teijeiro.<br />
<br />
# 3D printing was made by:<br />
[[File: Abaco.jpg | link=http://www.abaco.com.ar | center | 200px ]]<br />
<br />
<br />
<br />
==Acknowledgments==<br />
<br />
# Marcela Cristini for supporting our project through crowdfunding<br />
<br />
# Alan Bush for helping us in everything we need.<br />
<br />
# Diego Qurejeta for measuring our samples<br />
<br />
# Vattino's and Patop's Family for giving us a refrigerator.<br />
<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_attributionTeam:Buenos Aires/ attribution2013-10-27T22:56:43Z<p>Santiago: /* Design */</p>
<hr />
<div><div id="external"><br />
<br />
=Attributions=<br />
<br />
==Prototype Design==<br />
# The design of the physical device was performed by the industrial designers Romina Mathieu, Luciana Feo Mourelle and Adrián Teijeiro.<br />
<br />
# 3D printing was made by:<br />
[[File: Abaco.jpg | link=http://www.abaco.com.ar | center | 200px ]]<br />
<br />
# Some of the wiki pictures and images were made by Nicole Shrewsbury and Sebastian Vishnopolska. <br />
<br />
# Video, Photos and Audio were made by Gonzalo Moiguer, Rodrigo Melendez and Javier de Azkue.<br />
<br />
# Everything else, including experimental design, modeling, web and graphical design, has been done by the student members of the team supervised by the advisors and the instructors.<br />
<br />
==Acknowledgments==<br />
<br />
# Marcela Cristini for supporting our project through crowdfunding<br />
<br />
# Alan Bush for helping us in everything we need.<br />
<br />
# Diego Qurejeta for measuring our samples<br />
<br />
# Vattino's and Patop's Family for giving us a refrigerator.<br />
<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-27T22:55:43Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
<div id="inwiki"><br />
'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain information. Our central goal is to help solve this situation, so we thought of a '''biosensor that is cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on the top of the list of countries with high natural arsenic concentration in ground water of certain zones. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
Crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
<br />
<br />
{| class="wikitable" style="margin:auto; background: #FFF; border: 0px;"<br />
|+ Arsenic destribution<br />
|-<br />
|<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_summaryTeam:Buenos Aires/ summary2013-10-27T22:54:57Z<p>Santiago: /* To Drink Or Not To Drink */</p>
<hr />
<div><div id="external"><br />
<br />
= To Drink Or Not To Drink =<br />
<br />
<div id="inwiki"><br />
'''Contamination''' is a major and continuously growing worldwide problem. As only 2.5% of water is available for human consumption and just 1% of it supplies ecosystems and human populations, contamination in natural springs is a key issue to be addressed.<br />
<br />
At present, the spatial and temporal '''quantification of contaminants is limited''' by the difficulty in processing samples, remoteness of some of the locations and its associated costs. Moreover, the lack of centralization and systematization of data makes it very difficult to obtain information. Our central goal is to help solve this situation, so we thought of a '''biosensor that is cheap and easy to use''' by people without previous training or equipment. <br />
<br />
<br />
{| class="wikitable" style="background-color:#fff; margin:auto;"<br />
|+To Drink or Not To Drink<br />
|-<br />
|[[File:howworks.gif | link=Team:Buenos_Aires/_device]]<br />
|[[File:bairesGrupal.jpg | link=Team:Buenos_Aires/_students]]<br />
|}<br />
<br />
<br />
'''Arsenic''' is naturally present in soil and ground water in certain regions. '''Argentina, the United States, Bangladesh and Chile''' are on the top of the list of countries with high natural arsenic concentration in ground water of certain zones. Population without access to treated water living in these areas is '''exposed to Arsenic consumption'''. This derives in certain health complications such as skin lesions, skin, lung and bladder cancer, and gastro-intestinal and pulmonary disorders. <br />
<br />
<br />
----<br />
<br />
crowfunding video that explains the arsenic problem in a very intuitive manner(spanish version):<br />
<br />
{| class="wikitable" style="margin:auto;"<br />
|+ Sensar<br />
|-<br />
| <html><iframe left="50%" src="//player.vimeo.com/video/75360998" width="500" height="281" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> <br />
<p><a href="http://vimeo.com/75360998">SensAR - Biosensores de Contaminacion en Agua basados en Biologia Sintetica</a> </p> </html><br />
|}<br />
<br />
<br />
<br />
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|+ Arsenic destribution<br />
|-<br />
|<br />
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</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_res_rfpdegTeam:Buenos Aires/ res rfpdeg2013-09-27T19:43:08Z<p>Santiago: /* GFP under Arsenite Inducible Promoter */</p>
<hr />
<div><div id="external"><br />
<br />
= GFP under Arsenite Inducible Promoter =<br />
<br />
<br />
----<br />
<br />
<br />
'''Objective'''<br />
<br />
Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.<br />
<br />
<br />
'''Methods'''<br />
<br />
Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).<br />
<br />
<br />
'''Results'''<br />
<br />
As shown in the graphics below GFP production increases with the arsenite concentration. <br />
<br />
<br />
'''Conclusion'''<br />
<br />
The promoter is sensitive to different arsenite concentrations.<br />
<br />
<br />
[[File:Gfpinduccion.jpg|center|600px|]]<br />
<br />
[[File:Gfpampliacion2.jpg|center|600px|]]<br />
<br />
<br />
</div></div>Santiagohttp://2013.igem.org/File:Gfpampliacion2.jpgFile:Gfpampliacion2.jpg2013-09-27T19:42:31Z<p>Santiago: </p>
<hr />
<div></div>Santiagohttp://2013.igem.org/File:Gfp_ampliacion.jpgFile:Gfp ampliacion.jpg2013-09-27T19:25:36Z<p>Santiago: uploaded a new version of &quot;File:Gfp ampliacion.jpg&quot;</p>
<hr />
<div></div>Santiagohttp://2013.igem.org/File:Gfp_ampliacion.jpgFile:Gfp ampliacion.jpg2013-09-27T19:20:33Z<p>Santiago: uploaded a new version of &quot;File:Gfp ampliacion.jpg&quot;</p>
<hr />
<div></div>Santiagohttp://2013.igem.org/File:Gfp_ampliacion.jpgFile:Gfp ampliacion.jpg2013-09-27T19:19:37Z<p>Santiago: uploaded a new version of &quot;File:Gfp ampliacion.jpg&quot;</p>
<hr />
<div></div>Santiagohttp://2013.igem.org/File:Gfp_ampliacion.jpgFile:Gfp ampliacion.jpg2013-09-27T19:10:51Z<p>Santiago: uploaded a new version of &quot;File:Gfp ampliacion.jpg&quot;</p>
<hr />
<div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_mapaarsenicoTeam:Buenos Aires/ mapaarsenico2013-09-27T18:51:28Z<p>Santiago: /* Testing water samples from different locations */</p>
<hr />
<div><div id="external"><br />
<br />
=Testing water samples from different locations=<br />
<br />
Our project wouldn’t be fullfilled if we didn’t bring it to the society. To accomplish that, we are trying to make a map indicating the level of arsenite pollution in each location. However, we must know that due to the strange distribution of arsenic in groundwater,the detection of arsenic in a sample doesn't mean that the whole location is contamianted.<br />
<br />
<br />
[[File:Colecta_de_muestras_de_agua.jpg|center|800px]]<br />
<br />
<br />
We are collecting sample water from different towns in the nearby of Buenos Aires. In the near future, we are going to test this samples with our system.<br />
<br />
<br />
[[File:Mapa_gran_buenos_aires.jpg|center|800px]]<br />
<br />
<br />
</div>.</div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:48:54Z<p>Santiago: /* Parts Submitted */</p>
<hr />
<div><div id="external"><br />
<br />
<br />
= Parts Submitted =<br />
<br />
<br />
{| class="wikitable" style="margin: auto; text-align: center; color: black;"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2(not sent) <br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|700px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:45:55Z<p>Santiago: /* Part Submitted */</p>
<hr />
<div><div id="external"><br />
<br />
<br />
= Parts Submitted =<br />
<br />
<br />
{| class="wikitable" style="margin: auto; text-align: center; color: black;"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2<br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|700px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:45:42Z<p>Santiago: </p>
<hr />
<div><div id="external"><br />
<br />
<br />
= Part Submitted =<br />
<br />
<br />
{| class="wikitable" style="margin: auto; text-align: center; color: black;"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2<br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|700px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:43:53Z<p>Santiago: </p>
<hr />
<div><div id="external"><br />
{| class="wikitable" style="margin: auto; text-align: center; color: black;"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2<br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|700px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:43:25Z<p>Santiago: </p>
<hr />
<div><div id="external"><br />
{| class="wikitable" style="margin: auto; text-align: center; color: black;"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2<br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|800px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/Team:Buenos_Aires/_tabladepartesTeam:Buenos Aires/ tabladepartes2013-09-27T18:38:22Z<p>Santiago: </p>
<hr />
<div><div id="external"><br />
{| class="wikitable" style="text-align: center; color: black;|center"<br />
|'''Registry Code'''<br />
|'''Type'''<br />
|'''Sequence Description'''<br />
|'''Length'''<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106000 Bba_K1106000]<br />
|Composite<br />
|mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed<br />
|938 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106001 Bba_K1106001]<br />
|Composite<br />
|P22 c2 generator under PLL promoter<br />
|970 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106002 Bba_K1106002]<br />
|Composite<br />
|psp3 generator under lux pR promoter<br />
|437 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]<br />
|Reporter<br />
|mRFP generator under Ars promoter<br />
|1390 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]<br />
|Reporter<br />
|GFP generator under Ars promoter<br />
|1404 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106005 Bba_K1106005]<br />
|Composite<br />
|LuxR generator under a constitutive promoter<br />
|982 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106006 Bba_K1106006]<br />
|Reporter<br />
|Pulse generator part 3<br />
|1928 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106007 Bba_K1106007]<br />
|Composite<br />
|Pulse generator part 2<br />
|1415 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106008 Bba_K1106008]<br />
|Composite<br />
|Pulse generator part 1<br />
|1327 bp<br />
|-<br />
|[http://parts.igem.org/Part:BBa_K1106009 Bba_K1106009]<br />
|Generator<br />
|LuxR generator with a strong RBS<br />
|939 bp<br />
|-<br />
|}<br />
<br />
[[File:Partes_mandadas_con_rótulos.png|center|600px]]<br />
<br />
</div></div>Santiagohttp://2013.igem.org/File:Partes_mandadas_con_r%C3%B3tulos.pngFile:Partes mandadas con rótulos.png2013-09-27T18:37:55Z<p>Santiago: </p>
<hr />
<div></div>Santiago