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http://2013.igem.org/Team:SDU-Denmark/Tour51
Team:SDU-Denmark/Tour51
2014-01-08T13:18:56Z
<p>Shengry0716: </p>
<hr />
<div><br />
{{:Team:SDU-Denmark/core/header| }}<br />
<html><br />
<h2>Cloning</h2><br />
<h4>The beauty of creation<br />
</h4><br />
<br />
<p><br />
<br />
<span class="intro">To clarify the progression of our cloning</span>, the results will not be presented chronologically, but rather in a logical manner, starting with the simplest constructs. This is not an exhaustive list, but merely a presentation to clarify our work and give insight into our thoughts. Please consult our submitted parts-page (dig deeper), as well as our comprehensive protocols to gain full insight into our work. We have done our utmost to edit the gel photos in a responsible manner. Should questions arise, originals can be found in our protocols.<br />
</p><br />
<br />
<br><br />
<br />
<p><br />
<br />
<br />
<a class="popupImg alignRight" style="width:130px" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_Cloning_1.png" title="Figure 1 - The picture shows the colony PCR result for the cloning and subsequent transformation of pSB1C3-Plac-<i>dxs(E.coli)</i>. The gel picture shows the expected length around 2300 bp including verification primers."><br />
<img src="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_Cloning_1.png" <br />
<br />
style="width:130px" /><br />
Figure 1. <br />
</a><br />
<br />
<h4>pSB1C3-Plac-<span class="specialWord">dxs (E. coli)</span></h4><br />
<br />
<br />
<span class="intro">The <br />
<span class="specialWord">dxs </span>from<br />
<span class="specialWord"> <span class="specialWord">E. coli</span></span> was already present</span> in parts registry <br />
<br />
(<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K118000">BBa_K118000</a>)<br />
<br />
<br />
, which simplified our aim to increase expression of the gene. However, the middle part of the brick was unsequenced, so this was an obvious focal point. We successfully completed the sequencing and the full sequence has been entered into part registry (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088012" title="">BBa_K1088012</a>). USER cloning was used to clone PLac together with <span class="specialWord">E.coli dxs</span> in order to control the expression of Dxs. The picture shows the colony PCR result for the cloning and subsequent transformation. The gel picture shows the expected length around 2300 bp including verification primers <b>(Fig. 1)</b>. <br />
<br />
<br />
</p><br />
<br />
<br />
<br><br />
<br />
<br />
<p><br />
<h4>pSB1C3-Plac-dxs <span class="specialWord">(B. subtilis)</span></h4><br />
<br />
<br />
<span class="sourceReference"><span class="intro">The literature suggested </span></span><br />
<br />
<br />
<span class="tooltip"><br />
<span class="tooltipHeader">Source:</span><br />
Y. Zhao et al. Biosynthesis of isoprene in Escherichia Coli via methylerythritol phosphate (MEP) pathway.<br />
</span> <br />
<br />
that <span class="specialWord">dxs</span> from <span class="specialWord">B. subtilis</span> performed better than its <span class="specialWord">E. coli</span> counterpart. Therefore, a colony PCR was performed on the <span class="specialWord">B. subtilis</span> strain 168. USER cloning was used to produce the construct pSB1C3-Plac-dxs <span class="specialWord">(B. subtilis)</span>. Our tests of the construct unfortunately showed four restriction sites within the coding sequence <b>(Fig. 2)</b>.<br />
<br />
</p><br />
<br />
<p><br />
<span class="intro">Site directed mutagenesis</span> was performed to remove the restriction sites, which ensured that the gene complied with iGEM standards. The gel picture shows the expected length around 2000 bp <b>(Fig. 3)</b>. Full confirmed sequence can be found by following this link (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088011" title="">BBa_K1088011</a>).<br />
<br />
</p><br />
<br />
<br><br />
<br />
<p><br />
<h4>pSB1C3-lacI-Plac-<span class="specialWord">dxs (B. subtilis)</span></h4><br />
<br />
<span class="intro">Then, our focus moved to expression control:</span> Subsequent testing proved that the inherent amount of LacI (the repressor of the lactose promoter) in MG1655 was insufficient in the presence of our construct in a high-copy plasmid (see characterization). Therefore, <span class="specialWord">lacI</span> with an LVA tag was taken from parts registry (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a>) and cloned into our construct with its own promoter and terminator. pSB1C3-Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (B. subtilis)</span> was born. The gel-picture shows the result for the test digestion. Linearized plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp <b>(Fig. 4)</b>. This, too, was sequenced and may be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088013" title="">BBa_K1088013</a>). The brick allows us to overcome the first rate-limiting step of the MEP pathway in a regulatable manner.<br />
</p><br />
<br />
<br />
<div class="imageGallery galleryMedium alignCenter" style="height: 140px;"><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/f/f6/SDU2013_Cloning_2.png" title="Figure 2 - USER cloning was used to produce the construct pSB1C3-Plac-<i>dxs (B. subtilis)</i>. Our tests of the construct showed unfortunately two restriction sites within the coding sequence."><br />
<img src="https://static.igem.org/mediawiki/2013/2/24/SDU2013_Small_Cloning_2.png"><br />
Figure 2.<br />
</a><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/c/ce/SDU2013_Cloning_3.png" title="Figure 3 - Site directed mutagenesis was performed to remove the restriction sites in <i>dxs(B. subtilis)</i>, which ensured that the gene complied with iGEM standards. The gel picture shows the expected length around 2000 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/6/66/SDU2013_Small_Cloning_3.png"><br />
Figure 3.<br />
</a><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/b/bc/SDU2013_Cloning_4.png" title="Figure 4 - The gel-picture shows the result for the test digestion of pSB1C3-<i>LacI:LVA</i>-Plac-<i>dxs(B. subtilis)</i>. Linearized plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/5/56/SDU2013_Small_Cloning_4.png"><br />
Figure 4.<br />
</a><br />
&nbsp;<br />
</div><br />
<br />
<br />
<br />
<br />
<br><br><br />
<br />
<br />
<br />
<p><br />
<br />
<a class="popupImg alignRight" style="width:200px" href="https://static.igem.org/mediawiki/2013/5/52/SDU2013_Cloning_5.png" title="Figure 5 - The gel picture shows colony PCR result for the transformation with pSB1C3-Pcon-<i>LacI(N)<i/>-term-Plac-<i>dxs(B.subtilis)</i>. A band appeared just above 3000 bp as expected for this construct."><br />
<img src="https://static.igem.org/mediawiki/2013/5/52/SDU2013_Cloning_5.png" <br />
<br />
style="width:200px" /><br />
Figure 5. <br />
</a><br />
<br />
<br />
<span class="intro">Additionally, we observed indications</span> that LacI:LVA was an inferior repressor to MG1655’s own LacI. As a consequence, we performed yet another colony PCR to amplify natural LacI, and clone this into our construct in place of LacI:LVA. The gel picture shows colony PCR result for the transformation with pSB1C3-Pcon-<span class="specialWord">lacI(N)</span>-term-Plac-<span class="specialWord">dxs (B. sub)</span>. A band appeared just above 3000 bp as expected for this construct <b>(Fig. 5)</b>. The confirmed sequence can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088027" title="">BBa_K1088027</a>).<br />
<br />
<br />
</p><br />
<br />
<br />
<br />
<br />
<p><br />
<br />
<span class="intro">As a final test of this hypothesis</span>, the plasmid was transformed into KG22, an <span class="specialWord">E. coli</span> strain with natural abundance of LacI. This is further discussed in our characterization of LacI on the next page in the tour. <br />
<br />
</p><br />
<br />
<br />
<br><br />
<br />
<p><br />
<br />
<br />
<br />
<br />
<br />
<h4>pSB1C3-Para-HRT2</h4><br />
<br />
<a class="popupImg alignRight" style="width:160px" href="https://static.igem.org/mediawiki/2013/e/e3/SDU2013_Cloning_6.png" title="Figure 6 - The gel picture shows two bands. The band just above 2000 bp corresponds to the expected length of shipped pUC57 plasmid and the second band around 900 bp is the expected length for the coding HTR2 sequence."><br />
<img src="https://static.igem.org/mediawiki/2013/e/e3/SDU2013_Cloning_6.png" style="width:160px" /><br />
Figure 6. <br />
</a><br />
<br />
<span class="intro">GenStript performed the synthesis</span> of <span class="specialWord">HRT2</span> polyprenyltranferase with a flagtag added in the end of the sequence, an RBS and an arabinose promoter in front. Unfortunately, the stop codon of the gene was not removed, leaving the tag unfunctionable. The coding sequence of HRT2 was amplified with primers adding prefix and suffix to the sequence. The gel picture shows two bands. The band just above 2000 bp corresponds to the expected length of shipped pUC57 plasmid and the second band around 900 bp is the expected length for the coding HTR2 sequence <b>(Fig. 6)</b>. The basic part was sequenced, and the confirmed sequenced can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088003" title="">BBa_K1088003</a>).<br />
<br />
<br />
</p><br />
<br />
<br><br />
<br />
<br />
<p><br />
<br />
<a class="popupImg alignLeft" style="width:130px" href="https://static.igem.org/mediawiki/2013/9/9b/SDU2013_Cloning_7.png" title="Figure 7 - The colony PCR for the transformation with pSB1C3-Pcon-araC-term-Para-HRT2-FT shows a band just above 2500 bp as expected."><br />
<img src="https://static.igem.org/mediawiki/2013/9/9b/SDU2013_Cloning_7.png" style="width:130px" /><br />
Figure 7. <br />
</a><br />
<br />
<br />
<span class="intro">In order to assay optimized control</span> of the expression from the arabinose promoter and thus HTR2, we build and added an AraC device (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088017" title="">BBa_K1088017</a>). A colony PCR was performed on MG1655 to obtain <span class="specialWord">araC</span>, which was cloned into the construct with its own promoter and terminator. The construct design was: pSB1C3-Pcon-<span class="specialWord">araC</span>-term-Para-<span class="specialWord">HRT2</span>-FT. The colony PCR for the transformation with the construct shows a band just above 2500 bp as expected <b>(Fig. 7)</b>. The confirmed sequence can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088016" title="">BBa_K1088016</a>). <br />
<br />
<br />
<br />
</p><br />
<br />
<br><br />
<br />
<a class="popupImg alignRight" style="width:160px" href="https://static.igem.org/mediawiki/2013/5/53/SDU2013_Cloning_7.5.png" title="Figure 8 - The gel picture shows the result of a colony PCR for the cloning of Pcon-araC-term-Para-HRT2-FT into pSB1K3 and subsequent transformation. A band with the expected length around 2500 bp are found"><br />
<img src="https://static.igem.org/mediawiki/2013/5/53/SDU2013_Cloning_7.5.png" style="width:160px"><br />
Figure 8. <br />
</a><br />
<br />
<p><br />
<br />
<br />
<br />
<span class="intro">We had great difficulty</span> cloning the construct into pSB1A3, and therefore chose pSB1K3 as our plasmid for the production system. The cloning was immediately successful. The gel picture shows the result of a colony PCR with the expected length found at approximately 2500 bp <b>(Fig. 8)</b>.<br />
<br />
<br />
<br />
<br />
<br />
</p><br />
<br />
<br />
<br><br />
<br />
<br />
<br />
<p><br />
<h4>Reporter systems</h4><br />
<br />
<br />
<span class="intro">To check the expression profile</span> of our constructs, we used two distinct methods: <b>1)</b> For the <span class="specialWord">dxs</span> construct, protein fusion with a reporter gene, coupled with microscopy or FACS was used, and <b>2)</b> for the <span class="specialWord">HRT2</span> construct, Northern blots were performed. To see the results of these tests, see Characterization. <br />
<br />
</p><br />
<br />
<p><br />
<br />
<span class="intro">For our reporter system</span>, we produced pSB1C3-Pcon-<span class="specialWord">dxs</span> <span class="specialWord">(B. subtilis)</span>-AmilCP (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088006" title="">BBa_K1088006</a>). The AmilCP, however, proved to be a very poor fusion reporter gene, and we abandoned this construct to focus our attention on GFP. A number of constructs were successfully made, corresponding to the evolution of our design outlined above <b>(Fig. 9-11)</b>.<br />
<br />
</p><br />
<br />
<ul><br />
<br />
<p><br />
<li><span class="intro">pSB1C3-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. subtilis)</span>-linker-GFP</span> was succesfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088008" title="">BBa_K1088008</a>), as seen on <b>Figure 9.</b><br />
</li><br />
</p><p><br />
<li><span class="intro">pSB1C3-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(E. coli)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088007" title="">BBa_K1088007</a>), as seen on <b>Figure 9</b>. The gel picture shows the result of test digest with a band appearing around 5000 bp. The two constructs have been run next to each other. <br></li><br />
</p><p><br />
<li><span class="intro">pSB1C3-<span class="specialWord">LacI:LVA</span>-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. Subtilis)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088009" title="">BBa_K1088009</a>). <b>Figure 10</b> shows a test digest with the length of the insert corresponding to the expected length around 5000 bp.<br></li><br />
</p><p><br />
<li><span class="intro">pSB1C3-<span class="specialWord">lacI(N)</span>-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. Subtilis)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088026" title="">BBa_K1088026</a>). <b>Figure 11</b> shows a test digest with the length of the insert corresponding to the expected length around 4000 bp.<br></li><br />
</p><br />
<br />
</ul><br />
<br />
<p><br />
<br />
<div class="imageGallery galleryMedium alignCenter"><br />
<br />
<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_Cloning_8.png" title="Figure 9 - The gel picture shows the result for the test digestion of pSB1C3-Plac-<i>dxs(E.coli)</i>-Linker-GFP (first lane) and pSB1C3-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP (second lane). A band of the expected length, 5000 bp, appears in both lanes."><br />
<img src="https://static.igem.org/mediawiki/2013/7/77/SDU2013_Small_Cloning_8.png"></img>Figure 9.</a><br />
<br />
<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/d/de/SDU2013_Cloning_9.png" title="Figure 10 - The gel picture shows the result for the test digestion of pSB1C3-<i>LacI:LVA</i>-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP. Linearized plasmid has the expected length around 7000 bp and the insert around 5000 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/1/1e/SDU2013_Small_Cloning_9.png"></img>Figure 10.</a><br />
<br />
<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/9/95/SDU2013_Cloning_10.png" title="Figure 11 - The gel picture shows the result for the test digestion of pSB1C3-<i>LacI(N)</i>-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP. Linearized plasmid has the expected length around 6000 bp and the insert around 4000 bp. "><br />
<img src="https://static.igem.org/mediawiki/2013/8/88/SDU2013_Small_Cloning_10.png"></img>Figure 11.</a><br />
<br />
&nbsp;<br />
</div><br />
<br />
</p><br />
<br />
<br />
<br />
<p><br />
<br />
<a target="_blank" href="https://static.igem.org/mediawiki/2013/d/d5/SDU2013_Primer_List.pdf">A list of our primers</a><br />
<br />
</p><br />
<br />
<br />
<br />
<br />
<br />
</html><br />
{{:Team:SDU-Denmark/core/footer}}</div>
Shengry0716
http://2013.igem.org/Team:SDU-Denmark/Tour51
Team:SDU-Denmark/Tour51
2014-01-08T13:18:35Z
<p>Shengry0716: </p>
<hr />
<div><br />
<html><br />
<h2>Cloning</h2><br />
<h4>The beauty of creation<br />
</h4><br />
<br />
<p><br />
<br />
<span class="intro">To clarify the progression of our cloning</span>, the results will not be presented chronologically, but rather in a logical manner, starting with the simplest constructs. This is not an exhaustive list, but merely a presentation to clarify our work and give insight into our thoughts. Please consult our submitted parts-page (dig deeper), as well as our comprehensive protocols to gain full insight into our work. We have done our utmost to edit the gel photos in a responsible manner. Should questions arise, originals can be found in our protocols.<br />
</p><br />
<br />
<br><br />
<br />
<p><br />
<br />
<br />
<a class="popupImg alignRight" style="width:130px" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_Cloning_1.png" title="Figure 1 - The picture shows the colony PCR result for the cloning and subsequent transformation of pSB1C3-Plac-<i>dxs(E.coli)</i>. The gel picture shows the expected length around 2300 bp including verification primers."><br />
<img src="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_Cloning_1.png" <br />
<br />
style="width:130px" /><br />
Figure 1. <br />
</a><br />
<br />
<h4>pSB1C3-Plac-<span class="specialWord">dxs (E. coli)</span></h4><br />
<br />
<br />
<span class="intro">The <br />
<span class="specialWord">dxs </span>from<br />
<span class="specialWord"> <span class="specialWord">E. coli</span></span> was already present</span> in parts registry <br />
<br />
(<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K118000">BBa_K118000</a>)<br />
<br />
<br />
, which simplified our aim to increase expression of the gene. However, the middle part of the brick was unsequenced, so this was an obvious focal point. We successfully completed the sequencing and the full sequence has been entered into part registry (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088012" title="">BBa_K1088012</a>). USER cloning was used to clone PLac together with <span class="specialWord">E.coli dxs</span> in order to control the expression of Dxs. The picture shows the colony PCR result for the cloning and subsequent transformation. The gel picture shows the expected length around 2300 bp including verification primers <b>(Fig. 1)</b>. <br />
<br />
<br />
</p><br />
<br />
<br />
<br><br />
<br />
<br />
<p><br />
<h4>pSB1C3-Plac-dxs <span class="specialWord">(B. subtilis)</span></h4><br />
<br />
<br />
<span class="sourceReference"><span class="intro">The literature suggested </span></span><br />
<br />
<br />
<span class="tooltip"><br />
<span class="tooltipHeader">Source:</span><br />
Y. Zhao et al. Biosynthesis of isoprene in Escherichia Coli via methylerythritol phosphate (MEP) pathway.<br />
</span> <br />
<br />
that <span class="specialWord">dxs</span> from <span class="specialWord">B. subtilis</span> performed better than its <span class="specialWord">E. coli</span> counterpart. Therefore, a colony PCR was performed on the <span class="specialWord">B. subtilis</span> strain 168. USER cloning was used to produce the construct pSB1C3-Plac-dxs <span class="specialWord">(B. subtilis)</span>. Our tests of the construct unfortunately showed four restriction sites within the coding sequence <b>(Fig. 2)</b>.<br />
<br />
</p><br />
<br />
<p><br />
<span class="intro">Site directed mutagenesis</span> was performed to remove the restriction sites, which ensured that the gene complied with iGEM standards. The gel picture shows the expected length around 2000 bp <b>(Fig. 3)</b>. Full confirmed sequence can be found by following this link (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088011" title="">BBa_K1088011</a>).<br />
<br />
</p><br />
<br />
<br><br />
<br />
<p><br />
<h4>pSB1C3-lacI-Plac-<span class="specialWord">dxs (B. subtilis)</span></h4><br />
<br />
<span class="intro">Then, our focus moved to expression control:</span> Subsequent testing proved that the inherent amount of LacI (the repressor of the lactose promoter) in MG1655 was insufficient in the presence of our construct in a high-copy plasmid (see characterization). Therefore, <span class="specialWord">lacI</span> with an LVA tag was taken from parts registry (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a>) and cloned into our construct with its own promoter and terminator. pSB1C3-Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (B. subtilis)</span> was born. The gel-picture shows the result for the test digestion. Linearized plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp <b>(Fig. 4)</b>. This, too, was sequenced and may be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088013" title="">BBa_K1088013</a>). The brick allows us to overcome the first rate-limiting step of the MEP pathway in a regulatable manner.<br />
</p><br />
<br />
<br />
<div class="imageGallery galleryMedium alignCenter" style="height: 140px;"><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/f/f6/SDU2013_Cloning_2.png" title="Figure 2 - USER cloning was used to produce the construct pSB1C3-Plac-<i>dxs (B. subtilis)</i>. Our tests of the construct showed unfortunately two restriction sites within the coding sequence."><br />
<img src="https://static.igem.org/mediawiki/2013/2/24/SDU2013_Small_Cloning_2.png"><br />
Figure 2.<br />
</a><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/c/ce/SDU2013_Cloning_3.png" title="Figure 3 - Site directed mutagenesis was performed to remove the restriction sites in <i>dxs(B. subtilis)</i>, which ensured that the gene complied with iGEM standards. The gel picture shows the expected length around 2000 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/6/66/SDU2013_Small_Cloning_3.png"><br />
Figure 3.<br />
</a><br />
<br />
<a class="galleryImg" target="_blank"<br />
href="https://static.igem.org/mediawiki/2013/b/bc/SDU2013_Cloning_4.png" title="Figure 4 - The gel-picture shows the result for the test digestion of pSB1C3-<i>LacI:LVA</i>-Plac-<i>dxs(B. subtilis)</i>. Linearized plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/5/56/SDU2013_Small_Cloning_4.png"><br />
Figure 4.<br />
</a><br />
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<p><br />
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<a class="popupImg alignRight" style="width:200px" href="https://static.igem.org/mediawiki/2013/5/52/SDU2013_Cloning_5.png" title="Figure 5 - The gel picture shows colony PCR result for the transformation with pSB1C3-Pcon-<i>LacI(N)<i/>-term-Plac-<i>dxs(B.subtilis)</i>. A band appeared just above 3000 bp as expected for this construct."><br />
<img src="https://static.igem.org/mediawiki/2013/5/52/SDU2013_Cloning_5.png" <br />
<br />
style="width:200px" /><br />
Figure 5. <br />
</a><br />
<br />
<br />
<span class="intro">Additionally, we observed indications</span> that LacI:LVA was an inferior repressor to MG1655’s own LacI. As a consequence, we performed yet another colony PCR to amplify natural LacI, and clone this into our construct in place of LacI:LVA. The gel picture shows colony PCR result for the transformation with pSB1C3-Pcon-<span class="specialWord">lacI(N)</span>-term-Plac-<span class="specialWord">dxs (B. sub)</span>. A band appeared just above 3000 bp as expected for this construct <b>(Fig. 5)</b>. The confirmed sequence can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088027" title="">BBa_K1088027</a>).<br />
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<p><br />
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<span class="intro">As a final test of this hypothesis</span>, the plasmid was transformed into KG22, an <span class="specialWord">E. coli</span> strain with natural abundance of LacI. This is further discussed in our characterization of LacI on the next page in the tour. <br />
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<p><br />
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<h4>pSB1C3-Para-HRT2</h4><br />
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<a class="popupImg alignRight" style="width:160px" href="https://static.igem.org/mediawiki/2013/e/e3/SDU2013_Cloning_6.png" title="Figure 6 - The gel picture shows two bands. The band just above 2000 bp corresponds to the expected length of shipped pUC57 plasmid and the second band around 900 bp is the expected length for the coding HTR2 sequence."><br />
<img src="https://static.igem.org/mediawiki/2013/e/e3/SDU2013_Cloning_6.png" style="width:160px" /><br />
Figure 6. <br />
</a><br />
<br />
<span class="intro">GenStript performed the synthesis</span> of <span class="specialWord">HRT2</span> polyprenyltranferase with a flagtag added in the end of the sequence, an RBS and an arabinose promoter in front. Unfortunately, the stop codon of the gene was not removed, leaving the tag unfunctionable. The coding sequence of HRT2 was amplified with primers adding prefix and suffix to the sequence. The gel picture shows two bands. The band just above 2000 bp corresponds to the expected length of shipped pUC57 plasmid and the second band around 900 bp is the expected length for the coding HTR2 sequence <b>(Fig. 6)</b>. The basic part was sequenced, and the confirmed sequenced can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088003" title="">BBa_K1088003</a>).<br />
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</p><br />
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<p><br />
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<a class="popupImg alignLeft" style="width:130px" href="https://static.igem.org/mediawiki/2013/9/9b/SDU2013_Cloning_7.png" title="Figure 7 - The colony PCR for the transformation with pSB1C3-Pcon-araC-term-Para-HRT2-FT shows a band just above 2500 bp as expected."><br />
<img src="https://static.igem.org/mediawiki/2013/9/9b/SDU2013_Cloning_7.png" style="width:130px" /><br />
Figure 7. <br />
</a><br />
<br />
<br />
<span class="intro">In order to assay optimized control</span> of the expression from the arabinose promoter and thus HTR2, we build and added an AraC device (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088017" title="">BBa_K1088017</a>). A colony PCR was performed on MG1655 to obtain <span class="specialWord">araC</span>, which was cloned into the construct with its own promoter and terminator. The construct design was: pSB1C3-Pcon-<span class="specialWord">araC</span>-term-Para-<span class="specialWord">HRT2</span>-FT. The colony PCR for the transformation with the construct shows a band just above 2500 bp as expected <b>(Fig. 7)</b>. The confirmed sequence can be found here (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088016" title="">BBa_K1088016</a>). <br />
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</p><br />
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<a class="popupImg alignRight" style="width:160px" href="https://static.igem.org/mediawiki/2013/5/53/SDU2013_Cloning_7.5.png" title="Figure 8 - The gel picture shows the result of a colony PCR for the cloning of Pcon-araC-term-Para-HRT2-FT into pSB1K3 and subsequent transformation. A band with the expected length around 2500 bp are found"><br />
<img src="https://static.igem.org/mediawiki/2013/5/53/SDU2013_Cloning_7.5.png" style="width:160px"><br />
Figure 8. <br />
</a><br />
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<p><br />
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<span class="intro">We had great difficulty</span> cloning the construct into pSB1A3, and therefore chose pSB1K3 as our plasmid for the production system. The cloning was immediately successful. The gel picture shows the result of a colony PCR with the expected length found at approximately 2500 bp <b>(Fig. 8)</b>.<br />
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<p><br />
<h4>Reporter systems</h4><br />
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<br />
<span class="intro">To check the expression profile</span> of our constructs, we used two distinct methods: <b>1)</b> For the <span class="specialWord">dxs</span> construct, protein fusion with a reporter gene, coupled with microscopy or FACS was used, and <b>2)</b> for the <span class="specialWord">HRT2</span> construct, Northern blots were performed. To see the results of these tests, see Characterization. <br />
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</p><br />
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<p><br />
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<span class="intro">For our reporter system</span>, we produced pSB1C3-Pcon-<span class="specialWord">dxs</span> <span class="specialWord">(B. subtilis)</span>-AmilCP (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088006" title="">BBa_K1088006</a>). The AmilCP, however, proved to be a very poor fusion reporter gene, and we abandoned this construct to focus our attention on GFP. A number of constructs were successfully made, corresponding to the evolution of our design outlined above <b>(Fig. 9-11)</b>.<br />
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</p><br />
<br />
<ul><br />
<br />
<p><br />
<li><span class="intro">pSB1C3-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. subtilis)</span>-linker-GFP</span> was succesfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088008" title="">BBa_K1088008</a>), as seen on <b>Figure 9.</b><br />
</li><br />
</p><p><br />
<li><span class="intro">pSB1C3-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(E. coli)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088007" title="">BBa_K1088007</a>), as seen on <b>Figure 9</b>. The gel picture shows the result of test digest with a band appearing around 5000 bp. The two constructs have been run next to each other. <br></li><br />
</p><p><br />
<li><span class="intro">pSB1C3-<span class="specialWord">LacI:LVA</span>-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. Subtilis)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088009" title="">BBa_K1088009</a>). <b>Figure 10</b> shows a test digest with the length of the insert corresponding to the expected length around 5000 bp.<br></li><br />
</p><p><br />
<li><span class="intro">pSB1C3-<span class="specialWord">lacI(N)</span>-Plac-<span class="specialWord">dxs</span> <span class="specialWord">(B. Subtilis)</span>-linker-GFP</span> was successfully cloned and sequenced (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088026" title="">BBa_K1088026</a>). <b>Figure 11</b> shows a test digest with the length of the insert corresponding to the expected length around 4000 bp.<br></li><br />
</p><br />
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</ul><br />
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<p><br />
<br />
<div class="imageGallery galleryMedium alignCenter"><br />
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<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_Cloning_8.png" title="Figure 9 - The gel picture shows the result for the test digestion of pSB1C3-Plac-<i>dxs(E.coli)</i>-Linker-GFP (first lane) and pSB1C3-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP (second lane). A band of the expected length, 5000 bp, appears in both lanes."><br />
<img src="https://static.igem.org/mediawiki/2013/7/77/SDU2013_Small_Cloning_8.png"></img>Figure 9.</a><br />
<br />
<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/d/de/SDU2013_Cloning_9.png" title="Figure 10 - The gel picture shows the result for the test digestion of pSB1C3-<i>LacI:LVA</i>-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP. Linearized plasmid has the expected length around 7000 bp and the insert around 5000 bp."><br />
<img src="https://static.igem.org/mediawiki/2013/1/1e/SDU2013_Small_Cloning_9.png"></img>Figure 10.</a><br />
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<a class="galleryImg" target="_blank" href="https://static.igem.org/mediawiki/2013/9/95/SDU2013_Cloning_10.png" title="Figure 11 - The gel picture shows the result for the test digestion of pSB1C3-<i>LacI(N)</i>-Plac-<i>dxs(B.subtilis)</i>-Linker-GFP. Linearized plasmid has the expected length around 6000 bp and the insert around 4000 bp. "><br />
<img src="https://static.igem.org/mediawiki/2013/8/88/SDU2013_Small_Cloning_10.png"></img>Figure 11.</a><br />
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<a target="_blank" href="https://static.igem.org/mediawiki/2013/d/d5/SDU2013_Primer_List.pdf">A list of our primers</a><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/ianda
Team:Hong Kong CUHK/ianda
2013-10-29T03:05:50Z
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<h1>Prof. CHAN King Ming</h1><br />
<p2><br />
<p>Dr. King Ming Chan is an Associate Professor of School of Life Sciences and Director of Environmental Science Program, Faculty of Science, Chinese University. His research focuses on Aquatic Toxicology and Molecular Endocrinology using fish models. Recent studies are mainly on transgenic fish over-expressing somatolactin hormone for functional study and use of biomarkers of exposures to study the risk of environmental contaminants including pesticides, trace organics, nanoparticles, and metal ions in waters. Dr. Chan also studies the regulation of eukaryotic gene expression and transcription, focusing on understanding how metal-regulatory-element-binding transcription factors (MTFs) control metallothionein genes and how Pit-1s control pituitary polypeptide hormone genes.</p><br />
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<h1>Prof. CHAN Ting Fung</h1><br />
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<p>Dr. Ting-Fung Chan is an Assistant Professor in the School of Life Sciences, and the Deputy Director of the Centre for Microbial Genomics and Proteomics at the Chinese University of Hong Kong. He is the Coordinator of the CUHK iGEM team. His researches mainly focus on genomics and bioinformatics of microbial pathogens and complex human phenotypes. He enjoys talking about science, but even more so for toys: Mindstorms NXT, RX-178, 5D Mk-II, LX200-ACF, YAS-875EX, and iGEM. He wishes his students would comprehend the fun and excitement of all aforementioned, but if that is not possible then at the very least, iGEM.</p></p2><br />
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<h1>Prof. Kevin Yuk-Lap YIP</h1><br />
<p2><br />
<p>Dr. Kevin Yio is an assistant professor in the Deprtment of Computer Science and Engineering of the Chinese University of Hong Kong. He has been researching in the field of bioinformatics for 9 years, since he was a graduate student first in Hong Kong and later at Yale University. He is particularly interested in modeling and studying the interactions of biological objects in large-scale networks, such as protein-protein interaction, gene regulatory and metabolic networks. His major role in the CUHK iGEM team is to introduce the competition to engineering students, recruit interested and committed individuals, and help them to enrich the engineering aspects of the project.</p><br />
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<h1>Prof. Pun To, Douglas YUNG</h1><br />
<p2><p>Dr. Douglas Yung is an Assistant Professor in the CUHK Biomedical Engineering Program. He has been intrigued by biosensing and electronic interfacing of living cells since graduate school. On the biosensing side, his research group is developing technologies for rapid viability assessment of superbugs using a combination of electrochemical, optical techniques and MEMS devices. His team is also developing methods to interface living microbes with electronics at a micro- and nano-scale. As for this year&rsquo;s iGEM, he primarily provides support on the device and engineering side.</p><br />
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<h1>Prof. Kong Siu-Kai</h1><br />
<p2><br />
<p>Prof. KONG's laboratory studies the process of apoptosis. In particular, he is interested in elucidating how mitochondria regulate the apoptosis in 'normal' and drug-resistant cancer cells. He wonders how the resistance of mitochondria to MMP can explain the resistance of cancer cells to apoptosis induction, and he hopes to develop strategies for overcoming chemotherapy resistance by targeting tumor mitochondria. He believes that pharmacological interventions on mitochondria are good strategies to promote cell death in tumor cells. This research topic on apoptosis is therefore relevant to disease treatment with socioeconomic impacts.</p><br />
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<h1>Stephen</h1><br />
<p2><br />
<p>Full name: Leung King Pong, Stephen</p><br />
<p><br />
Programme of Study: Life Science PG1</p><br />
<p>Hi! I'm Stephen, currently a graduate student focusing on plant cell biology research. Apart from having a taste of synthetic biology, which constantly surprises me with fascinating ideas, I'm honoured to guide a team of enthusiastic students with great perseverance. I hope the team can enjoy themselves and I'm looking forward to seeing other new ideas in the coming competition!</p><br />
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<h1>Mary</h1><br />
<p2><br />
<p>Full name: ZHU Lin</p><br />
</p2><br />
<p><br />
<p2>Hi, I am a 2nd year PhD student studying Bioinformatics at CUHK. My research interests focus on cancer genetics and genomics, next generation sequencing analysis, and computational biology. I am very glad to join this team. I appreciate the opportunity to participate in the iGEM competition, which is an icon of innovation. In my spare time, I enjoy reading, travelling, playing badminton, dancing and hiking. <br />
</p2><br />
</p><br />
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<h1>Jacky</h1><br />
<p2><br />
<p>Full name: Jacky, Fong Chuen, Loo</p><br />
<p>Programme of study: Life Science PG3</p><br />
<p>Jacky is a third-year PhD student in School of Life Science at CUHK. His research focus is aptamer technology and its integration on bio-sensors development for downstream applications. He also studies the mechanism of multi-drug resistant cancer, focusing on the organelle-specific targeted drug action.</p><br />
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Shengry0716
http://2013.igem.org/File:Roy.jpg
File:Roy.jpg
2013-10-29T01:31:47Z
<p>Shengry0716: uploaded a new version of &quot;File:Roy.jpg&quot;</p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/ianda
Team:Hong Kong CUHK/ianda
2013-10-29T01:10:22Z
<p>Shengry0716: </p>
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<h2>Instructors and Advisors</h2><br />
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<h1>Prof. CHAN King Ming</h1><br />
<p2><br />
<p>Dr. King Ming Chan is an Associate Professor of School of Life Sciences and Director of Environmental Science Program, Faculty of Science, Chinese University. His research focuses on Aquatic Toxicology and Molecular Endocrinology using fish models. Recent studies are mainly on transgenic fish over-expressing somatolactin hormone for functional study and use of biomarkers of exposures to study the risk of environmental contaminants including pesticides, trace organics, nanoparticles, and metal ions in waters. Dr. Chan also studies the regulation of eukaryotic gene expression and transcription, focusing on understanding how metal-regulatory-element-binding transcription factors (MTFs) control metallothionein genes and how Pit-1s control pituitary polypeptide hormone genes.</p><br />
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<h1>Prof. CHAN Ting Fung</h1><br />
<p2><br />
<p>Dr. Ting-Fung Chan is an Assistant Professor in the School of Life Sciences, and the Deputy Director of the Centre for Microbial Genomics and Proteomics at the Chinese University of Hong Kong. He is the Coordinator of the CUHK iGEM team. His researches mainly focus on genomics and bioinformatics of microbial pathogens and complex human phenotypes. He enjoys talking about science, but even more so for toys: Mindstorms NXT, RX-178, 5D Mk-II, LX200-ACF, YAS-875EX, and iGEM. He wishes his students would comprehend the fun and excitement of all aforementioned, but if that is not possible then at the very least, iGEM.</p></p2><br />
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<div id="photo"><img src="https://static.igem.org/mediawiki/2013/1/1b/Kevin_Yip.JPG" height="100%" hspace="50" vspace="0" /></div><br />
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<h1>Prof. Kevin Yuk-Lap YIP</h1><br />
<p2><br />
<p>Dr. Kevin Yio is an assistant professor in the Deprtment of Computer Science and Engineering of the Chinese University of Hong Kong. He has been researching in the field of bioinformatics for 9 years, since he was a graduate student first in Hong Kong and later at Yale University. He is particularly interested in modeling and studying the interactions of biological objects in large-scale networks, such as protein-protein interaction, gene regulatory and metabolic networks. His major role in the CUHK iGEM team is to introduce the competition to engineering students, recruit interested and committed individuals, and help them to enrich the engineering aspects of the project.</p><br />
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<h1>Prof. Pun To, Douglas YUNG</h1><br />
<p2><p>Dr. Douglas Yung is an Assistant Professor in the CUHK Biomedical Engineering Program. He has been intrigued by biosensing and electronic interfacing of living cells since graduate school. On the biosensing side, his research group is developing technologies for rapid viability assessment of superbugs using a combination of electrochemical, optical techniques and MEMS devices. His team is also developing methods to interface living microbes with electronics at a micro- and nano-scale. As for this year&rsquo;s iGEM, he primarily provides support on the device and engineering side.</p><br />
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<h1>Prof. Kong Siu-Kai</h1><br />
<p2><br />
<p>Our laboratory studies the process of apoptosis. During apoptosis, enzymes such as caspase become active and orchestrate the death of the cell. One mechanism of caspase activation involves the release of cytochrome c and other apoptosis-inducing factors from damaging mitochondria.</p> <br />
<p>In particular, we are interested in elucidating how mitochondria regulate the apoptosis in 'normal' and drug-resistant cancer cells. We are wondering how the resistance of mitochondria to MMP can explain the resistance of cancer cells to apoptosis induction, and we hope to develop strategies for overcoming chemotherapy resistance by targeting tumor mitochondria. We believe that pharmacological interventions on mitochondria are good strategies to promote cell death in tumor cells. This research topic on apoptosis is therefore relevant to disease treatment with socioeconomic impacts.</p><br />
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<h1>Stephen</h1><br />
<p2><br />
<p>Full name: Leung King Pong, Stephen</p><br />
<p><br />
Programme of Study: Life Science PG1</p><br />
<p>Hi! I'm Stephen, currently a graduate student focusing on plant cell biology research. Apart from having a taste of synthetic biology, which constantly surprises me with fascinating ideas, I'm honoured to guide a team of enthusiastic students with great perseverance. I hope the team can enjoy themselves and I'm looking forward to seeing other new ideas in the coming competition!</p><br />
</p2><br />
</div><br />
</div><br />
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<div id="member" style="margin: 0px auto;"><br />
<div id="photo"><img src="https://static.igem.org/mediawiki/2013/b/b0/Zhulin.jpg" height="100%" hspace="50" /></div><br />
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<h1>Mary</h1><br />
<p2><br />
<p>Full name: ZHU Lin</p><br />
</p2><br />
<p><br />
<p2>Hi, I am a 2nd year PhD student studying Bioinformatics at CUHK. My research interests focus on cancer genetics and genomics, next generation sequencing analysis, and computational biology. I am very glad to join this team. I appreciate the opportunity to participate in the iGEM competition, which is an icon of innovation. In my spare time, I enjoy reading, travelling, playing badminton, dancing and hiking. <br />
</p2><br />
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<h1>Jacky</h1><br />
<p2><br />
<p>Full name: Jacky, Fong Chuen, Loo</p><br />
<p>Programme of study: Life Science PG3</p><br />
<p>Jacky is a third-year PhD student in School of Life Science at CUHK. His research focus is aptamer technology and its integration on bio-sensors development for downstream applications. He also studies the mechanism of multi-drug resistant cancer, focusing on the organelle-specific targeted drug action.</p><br />
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Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/File:Skk2.jpg
File:Skk2.jpg
2013-10-29T00:53:45Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/project
Team:Hong Kong CUHK/project
2013-10-28T17:08:34Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/futureapp
Team:Hong Kong CUHK/futureapp
2013-10-28T17:06:58Z
<p>Shengry0716: </p>
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<strong><br />
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<p2><p><strong><u>Control enzymatic reactions</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li><br />
<li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li><br />
<br />
</ul><br />
</p><br />
<p><strong><u>Bacterial Screening</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the BiFC</li><br />
<li>Load cells onto multi-well plates</li><br />
<li>Each well serves as a Pixel</li><br />
</ul><br />
</p><br />
<br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p><br />
<br />
<p><strong><u>Degradation of cellular proteins</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the Ubiquitin/26S proteasome system</li><br />
<li>Linking the voltage switch with E1/E2/E3 protein</li><br />
<li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li><br />
</ul><br />
</p><br />
<br />
<p><strong><u>Study genes using protein complementation</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Split genes into two parts, and insert into our system</li><br />
<li>Use voltage switch to control the split and refold of a gene.</li><br />
<li>Possible tool for the screening of new protein complementation compatible proteins.</li><br />
</ul><br />
</p><br />
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<p><strong><u>Study Membrane Potential of Organelles</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Target voltage switch system with BiFC onto the membrane of organelles interested</li><br />
<li>Obtain fluorescent signal</li><br />
<li>Estimate membrane potential</li><br />
</ul><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/futureapp
Team:Hong Kong CUHK/futureapp
2013-10-28T17:04:43Z
<p>Shengry0716: </p>
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<br />
<br />
<h3>Future Application</h3><br />
<br />
<br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Control enzymatic reactions</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li><br />
<li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li><br />
<br />
</ul><br />
</p><br />
<p><strong><u>Bacterial Screening</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the BiFC</li><br />
<li>Load cells onto multi-well plates</li><br />
<li>Each well serves as a Pixel</li><br />
</ul><br />
</p><br />
<br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p><br />
<br />
<p><strong><u>Degradation of cellular proteins</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the Ubiquitin/26S proteasome system</li><br />
<li>Linking the voltage switch with E1/E2/E3 protein</li><br />
<li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li><br />
</ul><br />
</p><br />
<br />
<p><strong><u>Study genes using protein complementation</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Split genes into two parts, and insert into our system</li><br />
<li>Use voltage switch to control the split and refold of a gene.</li><br />
<li>Possible tool for the screening of new protein complementation compatible proteins.</li><br />
</ul><br />
</p><br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/f/f3/Sry13.png" alt="2" /></p><br />
<br />
<p><strong><u>Study Membrane Potential of Organelles</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Target voltage switch system with BiFC onto the membrane of organelles interested</li><br />
<li>Obtain fluorescent signal</li><br />
<li>Estimate membrane potential</li><br />
</ul><br />
</p><br />
<br />
<br />
<br />
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<div id="backtop"><a href="#">TOP</a></div></div><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/futureapp
Team:Hong Kong CUHK/futureapp
2013-10-28T16:59:40Z
<p>Shengry0716: </p>
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<br />
<div id="partable"><br />
<br />
<br />
<h3>Future Application</h3><br />
<br />
<br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Control enzymatic reactions</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li><br />
<li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li><br />
<br />
</ul><br />
</p><br />
<p><strong><u>Bacterial Screening</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the BiFC</li><br />
<li>Load cells onto multi-well plates</li><br />
<li>Each well serves as a Pixel</li><br />
</ul><br />
</p><br />
<br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p><br />
<br />
<p><strong><u>Degradation of cellular proteins</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the Ubiquitin/26S proteasome system</li><br />
<li>Linking the voltage switch with E1/E2/E3 protein</li><br />
<li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li><br />
</ul><br />
</p><br />
<br />
<p><strong><u>Study genes using protein complementation</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Split genes into two parts, and insert into our system</li><br />
<li>Use voltage switch to control the split and refold of a gene.</li><br />
<li>Possible tool for the screening of new protein complementation compatible proteins.</li><br />
</ul><br />
</p><br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/f/f3/Sry13.png" alt="2" /></p><br />
<br />
<p><strong><u>Study Membrane Potential of Organelles</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Target voltage switch system with BiFC onto the membrane of organelles interested</li><br />
<li>Obtain fluorescent signal</li><br />
<li>Estimate membrane potential</li><br />
</ul><br />
</p><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
<div id="backtop"><a href="#">TOP</a></div></div><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/futureapp
Team:Hong Kong CUHK/futureapp
2013-10-28T16:53:03Z
<p>Shengry0716: </p>
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<br />
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<br />
<br />
<h3>Future Application</h3><br />
<br />
<br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Control enzymatic reactions</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li><br />
<li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li><br />
<br />
</ul><br />
</p><br />
<p><strong><u>Bacterial Screening</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the BiFC</li><br />
<li>Load cells onto multi-well plates</li><br />
<li>Each well serves as a Pixel</li><br />
</ul><br />
</p><br />
<br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p><br />
<br />
<p><strong><u>Degradation of cellular proteins</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the Ubiquitin/26S proteasome system</li><br />
<li>Linking the voltage switch with E1/E2/E3 protein</li><br />
<li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li><br />
</ul><br />
</p><br />
<br />
<p><strong><u>Study genes using protein complementation</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Split genes into two parts, and insert into our system</li><br />
<li>Use voltage switch to control the split and refold of a gene.</li><br />
<li>Possible tool for the screening of new protein complementation compatible proteins.</li><br />
</ul><br />
</p><br />
<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/f/f3/Sry13.png" alt="2" /></p><br />
<br />
<p><strong><u>Study Membrane Potential of Organelles</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Target voltage switch system with BiFC onto the membrane of organelles interested</li><br />
<li>Obtain fluorescent signal</li><br />
<li>Estimate membrane potential</li><br />
</ul><br />
</p><br />
<br />
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Shengry0716
http://2013.igem.org/File:Sry13.png
File:Sry13.png
2013-10-28T16:48:01Z
<p>Shengry0716: </p>
<hr />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/futureapp
Team:Hong Kong CUHK/futureapp
2013-10-28T16:36:57Z
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<br />
<br />
<h3>Future Application</h3><br />
<br />
<br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Control enzymatic reactions</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li><br />
<li>We are working on demonstrating this with laccase and dioxygenase, and also BiFC</li><br />
<br />
</ul><br />
</p><br />
<p><strong><u>Bacterial Screening</strong><br />
</u></p><br />
</strong><br />
<p><ul><br />
<li>Utilize the BiFC</li><br />
<li>Load cells onto multi-well plates</li><br />
<li>Each well serves as a Pixel</li><br />
</ul><br />
</p><br />
<br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p><br />
<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p><br />
<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p><br />
<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p><br />
<p>We investigate their response towards our project ideas.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p><br />
<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p><br />
<br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, <br />
50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)<br />
</p><br />
<br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/c/cb/Sry4.jpg" alt="4" width="565" height="294" /></p><br />
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<p align="center"><img width="553" height="311" src="http://https://static.igem.org/mediawiki/2013/3/3d/Sry5.jpg" alt="5" /></p><br />
<p>The data shows that synthetic biology is slightly more acceptable among non-believers. But the mean values lie above 3 for both views based on religious beliefs and personal opinion, thus synthetic biology is well accepted by our respondents.<br />
Lastly, we assess their response on the comic.<br />
<br />
</p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/1/1d/Sry6.jpg" alt="6" /></p><br />
<p>The comic is interesting for high school students as most of them rate it as 3 or above.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/d/de/Sry7.jpg" alt="7" /></p><br />
<p>Our comic delivers the content well, 97% of our respondents rate it as 3 or above.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/04/Sry8.jpg" alt="8" /></p><br />
<p>After reading the comic, most of the high school students have a better understanding on iGEM, synthetic biology or both.</p><br />
<br />
<br />
<strong><br />
<p>Parents (24 parents)</p><br />
</strong><br />
<p>During this open day event, we got the chance to meet some parents and conduct a survey. As most of them actually came to check on their children potential field of study in CUHK, we are so happy to learn that they are also enthusiastic about iGEM and synthetic biology.</p><br />
<p>We asked them on their familiarity to synthetic biology.</p><br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/Sry9.jpg" alt="9" width="555" height="320" /> </p><br />
<p>This result is consistent with response of high school students that came to our talk. (for question: do your parents understand what synthetic biology is?)</p><br />
<p>Parents are the gatekeepers of purchase, thus we investigate on their willing ness to buy and use products that is produced from synthetic biology.</p><br />
<br />
<br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/93/Sry10.jpg" alt="10" /></p><br />
<p>It is quite surprising that parents are willing to buy or use products from synthetic biology. They probably think that what has been in the market is safe to use.</p><br />
<p>We also explore their acceptability on using bacteria in daily lives. For example, using microorganism to remove cancer-causing substances from environment. This is to know whether they will welcome our project or not in the future.</p><br />
<br />
<br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/60/Sry11.jpg" alt="11" /></p><br />
<p>Most of the parents rated 3 for their acceptability, which means they accept synthetic biology but neither very supportive nor against it.</p><br />
<strong><p>Conclusion</p></strong><br />
<p>From the data that we got, many people still left uninformed about what is iGEM and synthetic biology. This is the same case for hazards of Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene. Our project idea is well received by our audiences. <br />
</p><br />
<p>One’s religious belief affects the way a person responds to the idea of synthetic biology. Non-believers most welcome synthetic biology; there is no need to hesitate to make iGEM and synthetic biology widespread in Hong Kong and Macau as most of them are non-believers.</p><p>Parents’ of high school students mostly do not know what synthetic biology is. Most of the parents are welcome to purchase and use products from synthetic biology. We have to start approaching these adults with the idea of synthetic biology, then they can educate their youngsters about it. Lastly, many agree that synthetic biology will be an integral part of a knowledge-based economy in the future, for Hong Kong and Macau.</p><br />
<p>Information two-way flow from surrounding to individual can be mediated through our comic. As our data shown that surrounding affects an individual (ex. Religion) and an individual understanding about an important topic like synthetic biology may contribute to the way he/she sees the surrounding situation, like the economy structure preferred.</p><br />
<br />
</p2><br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/File:Sry12.png
File:Sry12.png
2013-10-28T16:36:15Z
<p>Shengry0716: uploaded a new version of &quot;File:Sry12.png&quot;</p>
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Shengry0716
http://2013.igem.org/File:Sry12.png
File:Sry12.png
2013-10-28T16:33:53Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/humanpractice
Team:Hong Kong CUHK/humanpractice
2013-10-28T15:48:19Z
<p>Shengry0716: </p>
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<h3><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/hpintro">Introduction</a></h3><br />
<h3><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/hpHSHK">High School Students - Hong Kong</a></h3><br />
<h3><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/hpHSM">High School Students - Macau</a></h3><br />
<h3><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/hpUHK">University Students - Hong Kong</a></h3><br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/openday
Team:Hong Kong CUHK/openday
2013-10-28T15:36:08Z
<p>Shengry0716: </p>
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<p>This open day aims on introducing CUHK programs to high school students. We also indirectly promoting Engineering and Life Sciences programs to the students as we emphasized the fact that by being enrolled to either Engineering or Life Sciences programs, they will get the chance to join iGEM competition. </p><br />
<p>We informed them on what is synthetic biology, iGEM and our project.</p><br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Survey Analysis Hong Kong High School Students (234 students) at Open Day Booth</strong><br />
</u></p><br />
</strong><br />
<p>Firstly, we explore how well they know about iGEM and synthetic biology.</p><br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/9/9a/Sry1.jpg" alt="1" /></p><br />
<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p><br />
<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p><br />
<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p><br />
<p>We investigate their response towards our project ideas.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p><br />
<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p><br />
<br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, <br />
50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)<br />
</p><br />
<br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/c/cb/Sry4.jpg" alt="4" width="565" height="294" /></p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="311" src="http://https://static.igem.org/mediawiki/2013/3/3d/Sry5.jpg" alt="5" /></p><br />
<p>The data shows that synthetic biology is slightly more acceptable among non-believers. But the mean values lie above 3 for both views based on religious beliefs and personal opinion, thus synthetic biology is well accepted by our respondents.<br />
Lastly, we assess their response on the comic.<br />
<br />
</p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/1/1d/Sry6.jpg" alt="6" /></p><br />
<p>The comic is interesting for high school students as most of them rate it as 3 or above.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/d/de/Sry7.jpg" alt="7" /></p><br />
<p>Our comic delivers the content well, 97% of our respondents rate it as 3 or above.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/04/Sry8.jpg" alt="8" /></p><br />
<p>After reading the comic, most of the high school students have a better understanding on iGEM, synthetic biology or both.</p><br />
<br />
<br />
<strong><br />
<p>Parents (24 parents)</p><br />
</strong><br />
<p>During this open day event, we got the chance to meet some parents and conduct a survey. As most of them actually came to check on their children potential field of study in CUHK, we are so happy to learn that they are also enthusiastic about iGEM and synthetic biology.</p><br />
<p>We asked them on their familiarity to synthetic biology.</p><br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/Sry9.jpg" alt="9" width="555" height="320" /> </p><br />
<p>This result is consistent with response of high school students that came to our talk. (for question: do your parents understand what synthetic biology is?)</p><br />
<p>Parents are the gatekeepers of purchase, thus we investigate on their willing ness to buy and use products that is produced from synthetic biology.</p><br />
<br />
<br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/93/Sry10.jpg" alt="10" /></p><br />
<p>It is quite surprising that parents are willing to buy or use products from synthetic biology. They probably think that what has been in the market is safe to use.</p><br />
<p>We also explore their acceptability on using bacteria in daily lives. For example, using microorganism to remove cancer-causing substances from environment. This is to know whether they will welcome our project or not in the future.</p><br />
<br />
<br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/60/Sry11.jpg" alt="11" /></p><br />
<p>Most of the parents rated 3 for their acceptability, which means they accept synthetic biology but neither very supportive nor against it.</p><br />
<strong><p>Conclusion</p></strong><br />
<p>From the data that we got, many people still left uninformed about what is iGEM and synthetic biology. This is the same case for hazards of Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene. Our project idea is well received by our audiences. <br />
</p><br />
<p>One’s religious belief affects the way a person responds to the idea of synthetic biology. Non-believers most welcome synthetic biology; there is no need to hesitate to make iGEM and synthetic biology widespread in Hong Kong and Macau as most of them are non-believers.</p><p>Parents’ of high school students mostly do not know what synthetic biology is. Most of the parents are welcome to purchase and use products from synthetic biology. We have to start approaching these adults with the idea of synthetic biology, then they can educate their youngsters about it. Lastly, many agree that synthetic biology will be an integral part of a knowledge-based economy in the future, for Hong Kong and Macau.</p><br />
<p>Information two-way flow from surrounding to individual can be mediated through our comic. As our data shown that surrounding affects an individual (ex. Religion) and an individual understanding about an important topic like synthetic biology may contribute to the way he/she sees the surrounding situation, like the economy structure preferred.</p><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/openday
Team:Hong Kong CUHK/openday
2013-10-28T15:35:06Z
<p>Shengry0716: </p>
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<h3>CUHK Open Day</h3><br />
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<p>This open day aims on introducing CUHK programs to high school students. We also indirectly promoting Engineering and Life Sciences programs to the students as we emphasized the fact that by being enrolled to either Engineering or Life Sciences programs, they will get the chance to join iGEM competition. </p><br />
<p>We informed them on what is synthetic biology, iGEM and our project.</p><br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Survey Analysis Hong Kong High School Students (234 students) at Open Day Booth</strong><br />
</u></p><br />
</strong><br />
<p>Firstly, we explore how well they know about iGEM and synthetic biology.</p><br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/9/9a/Sry1.jpg" alt="1" /></p><br />
<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p><br />
<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p><br />
<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p><br />
<p>We investigate their response towards our project ideas.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p><br />
<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p><br />
<br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, <br />
50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)<br />
</p><br />
<br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/c/cb/Sry4.jpg" alt="4" width="565" height="294" /></p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="311" src="http://https://static.igem.org/mediawiki/2013/3/3d/Sry5.jpg" alt="5" /></p><br />
<p>The data shows that synthetic biology is slightly more acceptable among non-believers. But the mean values lie above 3 for both views based on religious beliefs and personal opinion, thus synthetic biology is well accepted by our respondents.<br />
Lastly, we assess their response on the comic.<br />
<br />
</p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/1/1d/Sry6.jpg" alt="6" /></p><br />
<p>The comic is interesting for high school students as most of them rate it as 3 or above.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/d/de/Sry7.jpg" alt="7" /></p><br />
<p>Our comic delivers the content well, 97% of our respondents rate it as 3 or above.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/04/Sry8.jpg" alt="8" /></p><br />
<p>After reading the comic, most of the high school students have a better understanding on iGEM, synthetic biology or both.</p><br />
<br />
<br />
<strong><br />
<p>Parents (24 parents)</p><br />
</strong><br />
<p>During this open day event, we got the chance to meet some parents and conduct a survey. As most of them actually came to check on their children potential field of study in CUHK, we are so happy to learn that they are also enthusiastic about iGEM and synthetic biology.</p><br />
<p>We asked them on their familiarity to synthetic biology.</p><br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/Sry9.jpg" alt="9" width="555" height="320" /> </p><br />
<p>This result is consistent with response of high school students that came to our talk. (for question: do your parents understand what synthetic biology is?)</p><br />
<p>Parents are the gatekeepers of purchase, thus we investigate on their willing ness to buy and use products that is produced from synthetic biology.</p><br />
<br />
<br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/93/Sry10.jpg" alt="10" /></p><br />
<p>It is quite surprising that parents are willing to buy or use products from synthetic biology. They probably think that what has been in the market is safe to use.</p><br />
<p>We also explore their acceptability on using bacteria in daily lives. For example, using microorganism to remove cancer-causing substances from environment. This is to know whether they will welcome our project or not in the future.</p><br />
<br />
<br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/60/Sry11.jpg" alt="11" /></p><br />
<p>Most of the parents rated 3 for their acceptability, which means they accept synthetic biology but neither very supportive nor against it.</p><br />
<strong><p>Conclusion</p></strong><br />
<p>From the data that we got, many people still left uninformed about what is iGEM and synthetic biology. This is the same case for hazards of Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene. Our project idea is well received by our audiences. <br />
</p><br />
<p>One’s religious belief affects the way a person responds to the idea of synthetic biology. Non-believers most welcome synthetic biology; there is no need to hesitate to make iGEM and synthetic biology widespread in Hong Kong and Macau as most of them are non-believers.</p><p>Parents’ of high school students mostly do not know what synthetic biology is. Most of the parents are welcome to purchase and use products from synthetic biology. We have to start approaching these adults with the idea of synthetic biology, then they can educate their youngsters about it. Lastly, many agree that synthetic biology will be an integral part of a knowledge-based economy in the future, for Hong Kong and Macau.</p><br />
<p>Information two-way flow from surrounding to individual can be mediated through our comic. As our data shown that surrounding affects an individual (ex. Religion) and an individual understanding about an important topic like synthetic biology may contribute to the way he/she sees the surrounding situation, like the economy structure preferred.</p><br />
<br />
</p2><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/openday
Team:Hong Kong CUHK/openday
2013-10-28T15:34:18Z
<p>Shengry0716: </p>
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<br />
<h3>CUHK Open Day</h3><br />
<br />
<p>This open day aims on introducing CUHK programs to high school students. We also indirectly promoting Engineering and Life Sciences programs to the students as we emphasized the fact that by being enrolled to either Engineering or Life Sciences programs, they will get the chance to join iGEM competition. </p><br />
<p>We informed them on what is synthetic biology, iGEM and our project.</p><br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Survey Analysis Hong Kong High School Students (234 students) at Open Day Booth</strong><br />
</u></p><br />
</strong><br />
<p>Firstly, we explore how well they know about iGEM and synthetic biology.</p><br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/9/9a/Sry1.jpg" alt="1" /></p><br />
<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p><br />
<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p><br />
<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p><br />
<p>We investigate their response towards our project ideas.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p><br />
<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p><br />
<br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, <br />
50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)<br />
</p><br />
<br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/c/cb/Sry4.jpg" alt="4" width="565" height="294" /></p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="311" src="http://https://static.igem.org/mediawiki/2013/3/3d/Sry5.jpg" alt="5" /></p><br />
<p>The data shows that synthetic biology is slightly more acceptable among non-believers. But the mean values lie above 3 for both views based on religious beliefs and personal opinion, thus synthetic biology is well accepted by our respondents.<br />
Lastly, we assess their response on the comic.<br />
<br />
</p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/1/1d/Sry6.jpg" alt="6" /></p><br />
<p>The comic is interesting for high school students as most of them rate it as 3 or above.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/d/de/Sry7.jpg" alt="7" /></p><br />
<p>Our comic delivers the content well, 97% of our respondents rate it as 3 or above.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/04/Sry8.jpg" alt="8" /></p><br />
<p>After reading the comic, most of the high school students have a better understanding on iGEM, synthetic biology or both.</p><br />
<br />
<br />
<strong><br />
<p>Parents (24 parents)</p><br />
</strong><br />
<p>During this open day event, we got the chance to meet some parents and conduct a survey. As most of them actually came to check on their children potential field of study in CUHK, we are so happy to learn that they are also enthusiastic about iGEM and synthetic biology.</p><br />
<p>We asked them on their familiarity to synthetic biology.</p><br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/Sry9.jpg" alt="9" width="555" height="320" /> </p><br />
<p>This result is consistent with response of high school students that came to our talk. (for question: do your parents understand what synthetic biology is?)</p><br />
<p>Parents are the gatekeepers of purchase, thus we investigate on their willing ness to buy and use products that is produced from synthetic biology.</p><br />
<br />
<br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/93/Sry10.jpg" alt="10" /></p><br />
<p>It is quite surprising that parents are willing to buy or use products from synthetic biology. They probably think that what has been in the market is safe to use.</p><br />
<p>We also explore their acceptability on using bacteria in daily lives. For example, using microorganism to remove cancer-causing substances from environment. This is to know whether they will welcome our project or not in the future.</p><br />
<br />
<br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/60/Sry11.jpg" alt="11" /></p><br />
<p>Most of the parents rated 3 for their acceptability, which means they accept synthetic biology but neither very supportive nor against it.</p><br />
<strong><p>Conclusion</p></strong><br />
<p>From the data that we got, many people still left uninformed about what is iGEM and synthetic biology. This is the same case for hazards of Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene. Our project idea is well received by our audiences. <br />
</p><br />
<p>One’s religious belief affects the way a person responds to the idea of synthetic biology. Non-believers most welcome synthetic biology; there is no need to hesitate to make iGEM and synthetic biology widespread in Hong Kong and Macau as most of them are non-believers.</p><p>Parents’ of high school students mostly do not know what synthetic biology is. Most of the parents are welcome to purchase and use products from synthetic biology. We have to start approaching these adults with the idea of synthetic biology, then they can educate their youngsters about it. Lastly, many agree that synthetic biology will be an integral part of a knowledge-based economy in the future, for Hong Kong and Macau.</p><br />
<p>Information two-way flow from surrounding to individual can be mediated through our comic. As our data shown that surrounding affects an individual (ex. Religion) and an individual understanding about an important topic like synthetic biology may contribute to the way he/she sees the surrounding situation, like the economy structure preferred.</p><br />
<br />
</p2><br />
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Shengry0716
http://2013.igem.org/File:Sry11.jpg
File:Sry11.jpg
2013-10-28T15:31:01Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry10.jpg
File:Sry10.jpg
2013-10-28T15:28:57Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry9.jpg
File:Sry9.jpg
2013-10-28T15:26:20Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry8.jpg
File:Sry8.jpg
2013-10-28T15:22:51Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry7.jpg
File:Sry7.jpg
2013-10-28T15:18:09Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry6.jpg
File:Sry6.jpg
2013-10-28T15:14:21Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/File:Sry5.jpg
File:Sry5.jpg
2013-10-28T15:12:50Z
<p>Shengry0716: </p>
<hr />
<div></div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/openday
Team:Hong Kong CUHK/openday
2013-10-28T15:01:24Z
<p>Shengry0716: </p>
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<h3>CUHK Open Day</h3><br />
<br />
<p>This open day aims on introducing CUHK programs to high school students. We also indirectly promoting Engineering and Life Sciences programs to the students as we emphasized the fact that by being enrolled to either Engineering or Life Sciences programs, they will get the chance to join iGEM competition. </p><br />
<p>We informed them on what is synthetic biology, iGEM and our project.</p><br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Survey Analysis Hong Kong High School Students (234 students) at Open Day Booth</strong><br />
</u></p><br />
</strong><br />
<p>Firstly, we explore how well they know about iGEM and synthetic biology.</p><br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/9/9a/Sry1.jpg" alt="1" /></p><br />
<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p><br />
<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p><br />
<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p><br />
<p>We investigate their response towards our project ideas.</p><br />
<br />
<br />
<br />
<br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p><br />
<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p><br />
<br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, <br />
50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)<br />
</p><br />
<br />
<br />
<br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/c/cb/Sry4.jpg" alt="4" width="565" height="294" /></p><br />
<br />
<br />
<br />
<br />
<p>We have a close to 50:50 responses for this question. It is advisable to change our delivery method and details if we want to outreach these details to the public.</p><br />
<p>We have obtained results based on individual view of synthetic biology and our project.<br /><br />
The following results are obtained by discussing synthetic biology correlation from one&rsquo;s environment.</p><br />
<p>From home, what makes information is well known is by involving family members to spread the knowledge and news. Unfortunately, the majority of parents do not know what synthetic biology is. This is shown in the following results.</p><br />
<p align="center"><img width="553" height="311" src="https://static.igem.org/mediawiki/2013/b/b1/HpHSHK_clip_image010.jpg" alt="5" /></p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/e/e9/HpHSHK_clip_image012.jpg" alt="6" /></p><br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, 50% Hong Kong&rsquo; population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the students is illustrated below.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/a/a2/HpHSHK_clip_image014.jpg" alt="7" /></p><br />
<p>The results indicated that, there is various insight of synthetic biology among different religious belief. Non-believers are most welcome towards synthetic biology.</p><br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/0c/HpHSHK_clip_image016.jpg" alt="8" /></p><br />
<p>The results from religion and personal point of view are similar. This shows that high school student&rsquo;s view on synthetic biology relates directly to their religious belief.</p><br />
<p>As a budding field, synthetic biology will be a very integral part of a knowledge-based economy in the future. Hong Kong, being a service-oriented economy has started to have some knowledge-based economic sectors. We asked the high school students on which one they prefer, knowledge-based or service-oriented?</p><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/1/1b/HpHSHK_clip_image018.jpg" alt="9" width="555" height="320" /> </p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/a/a6/HpHSHK_clip_image020.jpg" alt="10" /></p><br />
<p>The response is 50:50 for favoring knowledge-based or service-oriented economy. The majority of students believe that synthetic biology will be an integral part of knowledge-based economy.</p><br />
<p>Lastly, we also asked their interest in joining iGEM.</p><br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/69/HpHSHK_clip_image022.jpg" alt="11" /></p><br />
<p>Majority of Hong Kong High School students are interested in joining iGEM in the future.</p><br />
<p>Comments about out project: What do you think of using bacteria to degrade cancer-causing pollutants effectively?</p><br />
<p><ul><br />
<li>Acceptable</li><br />
<li>Beneficial</li><br />
<li>May prevent death but can be dangerous to be used</li><br />
<li>Great</li><br />
<li>Interesting</li><br />
<li>Positive</li><br />
<li>Worth to try</li><br />
<li>Society&rsquo;s benefit</li><br />
</ul><br />
</p></p2><br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/File:Sry4.jpg
File:Sry4.jpg
2013-10-28T15:00:30Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/File:Sry3.jpg
File:Sry3.jpg
2013-10-28T14:57:24Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/File:Sry2.jpg
File:Sry2.jpg
2013-10-28T14:52:42Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/File:Sry1.jpg
File:Sry1.jpg
2013-10-28T14:46:33Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/openday
Team:Hong Kong CUHK/openday
2013-10-28T14:38:31Z
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<h3>CUHK Open Day</h3><br />
<br />
<p>This open day aims on introducing CUHK programs to high school students. We also indirectly promoting Engineering and Life Sciences programs to the students as we emphasized the fact that by being enrolled to either Engineering or Life Sciences programs, they will get the chance to join iGEM competition. </p><br />
<p>We informed them on what is synthetic biology, iGEM and our project.</p><br />
<br />
<br />
<strong><br />
<br />
<p2><p><strong><u>Survey Analysis Hong Kong High School Students (234 students) at Open Day Booth</strong><br />
</u></p><br />
</strong><br />
<p>Firstly, we explore how well they know about iGEM and synthetic biology.</p><br />
<p align="center"><img width="553" height="347" src="https://static.igem.org/mediawiki/2013/4/4e/HpHSHK_clip_image002.jpg" alt="1" /></p><br />
<p>As shown on the graph above, more than 50% of our Hong Kong high school students have ever heard of neither synthetic biology nor iGEM. Amusingly, 35% have heard of synthetic biology before.</p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/94/HpHSHK_clip_image004.jpg" alt="2" /></p><br />
<p>Despite of their carcinogenicity, Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene are not well known among Hong Kong High School students.</p><br />
<p><strong>After the Talk</strong><br /><br />
After the talk, we handed out another survey to measure their understanding and response on certain topics to be investigated.</p><br />
<p>If we have successfully built a good understanding and interest of synthetic biology in our audience, they should be willing to discover more about synthetic biology in the future.</p><br />
<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/f/fe/HpHSHK_clip_image006.png" alt="3" /></p><br />
<p>Most of the students are deeply interested and going to seek further about synthetic biology.</p><br />
<p>We want to enhance awareness the hazards of Benzo[a]pyrene and Polycyclic Aromatic Hydrocarbon through our talk. </p><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/a/a2/HpHSHK_clip_image008.jpg" alt="4" width="565" height="294" /></p><br />
<p>We have a close to 50:50 responses for this question. It is advisable to change our delivery method and details if we want to outreach these details to the public.</p><br />
<p>We have obtained results based on individual view of synthetic biology and our project.<br /><br />
The following results are obtained by discussing synthetic biology correlation from one&rsquo;s environment.</p><br />
<p>From home, what makes information is well known is by involving family members to spread the knowledge and news. Unfortunately, the majority of parents do not know what synthetic biology is. This is shown in the following results.</p><br />
<p align="center"><img width="553" height="311" src="https://static.igem.org/mediawiki/2013/b/b1/HpHSHK_clip_image010.jpg" alt="5" /></p><br />
<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/e/e9/HpHSHK_clip_image012.jpg" alt="6" /></p><br />
<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly, 50% Hong Kong&rsquo; population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the students is illustrated below.</p><br />
<p>&nbsp;</p><br />
<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/a/a2/HpHSHK_clip_image014.jpg" alt="7" /></p><br />
<p>The results indicated that, there is various insight of synthetic biology among different religious belief. Non-believers are most welcome towards synthetic biology.</p><br />
<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/0c/HpHSHK_clip_image016.jpg" alt="8" /></p><br />
<p>The results from religion and personal point of view are similar. This shows that high school student&rsquo;s view on synthetic biology relates directly to their religious belief.</p><br />
<p>As a budding field, synthetic biology will be a very integral part of a knowledge-based economy in the future. Hong Kong, being a service-oriented economy has started to have some knowledge-based economic sectors. We asked the high school students on which one they prefer, knowledge-based or service-oriented?</p><br />
<p align="center"><img src="https://static.igem.org/mediawiki/2013/1/1b/HpHSHK_clip_image018.jpg" alt="9" width="555" height="320" /> </p><br />
<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/a/a6/HpHSHK_clip_image020.jpg" alt="10" /></p><br />
<p>The response is 50:50 for favoring knowledge-based or service-oriented economy. The majority of students believe that synthetic biology will be an integral part of knowledge-based economy.</p><br />
<p>Lastly, we also asked their interest in joining iGEM.</p><br />
<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/69/HpHSHK_clip_image022.jpg" alt="11" /></p><br />
<p>Majority of Hong Kong High School students are interested in joining iGEM in the future.</p><br />
<p>Comments about out project: What do you think of using bacteria to degrade cancer-causing pollutants effectively?</p><br />
<p><ul><br />
<li>Acceptable</li><br />
<li>Beneficial</li><br />
<li>May prevent death but can be dangerous to be used</li><br />
<li>Great</li><br />
<li>Interesting</li><br />
<li>Positive</li><br />
<li>Worth to try</li><br />
<li>Society&rsquo;s benefit</li><br />
</ul><br />
</p></p2><br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/File:HpUI_clip_image010.jpg
File:HpUI clip image010.jpg
2013-10-28T14:25:19Z
<p>Shengry0716: uploaded a new version of &quot;File:HpUI clip image010.jpg&quot;</p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/gallery/srybackup
Team:Hong Kong CUHK/gallery/srybackup
2013-10-28T13:22:29Z
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(function($,sr){<br />
// debouncing function from John Hann<br />
// http://unscriptable.com/index.php/2009/03/20/debouncing-javascript-methods/<br />
var debounce = function (func, threshold, execAsap) {<br />
var timeout;<br />
return function debounced () {<br />
var obj = this, args = arguments;<br />
function delayed () {<br />
if (!execAsap)<br />
func.apply(obj, args);<br />
timeout = null;<br />
};<br />
if (timeout)<br />
clearTimeout(timeout);<br />
else if (execAsap)<br />
func.apply(obj, args);<br />
timeout = setTimeout(delayed, threshold || 100);<br />
};<br />
}<br />
//smartresize<br />
jQuery.fn[sr] = function(fn){ return fn ? this.bind('resize', debounce(fn)) : this.trigger(sr); };<br />
})(jQuery,'smartresize');<br />
</script><br />
<script type="text/javascript"> <br />
$(function() {<br />
//check if the user made the<br />
//mistake to open it with IE<br />
var ie = false;<br />
if ($.browser.msie)<br />
ie = true;<br />
//flag to control the click event<br />
var flg_click = true;<br />
//the wrapper<br />
var $im_wrapper = $('#im_wrapper');<br />
//the thumbs<br />
var $thumbs = $im_wrapper.children('div');<br />
//all the images<br />
var $thumb_imgs = $thumbs.find('img');<br />
//number of images<br />
var nmb_thumbs = $thumbs.length;<br />
//image loading status<br />
var $im_loading = $('#im_loading');<br />
//the next and previous buttons<br />
var $im_next = $('#im_next');<br />
var $im_prev = $('#im_prev');<br />
//number of thumbs per line<br />
var per_line = 6;<br />
//number of thumbs per column<br />
var per_col = Math.ceil(nmb_thumbs/per_line)<br />
//index of the current thumb<br />
var current = -1;<br />
//mode = grid | single<br />
var mode = 'grid';<br />
//an array with the positions of the thumbs<br />
//we will use it for the navigation in single mode<br />
var positionsArray = [];<br />
for(var i = 0; i < nmb_thumbs; ++i)<br />
positionsArray[i]=i;<br />
<br />
<br />
//preload all the images<br />
$im_loading.show();<br />
var loaded = 0;<br />
$thumb_imgs.each(function(){<br />
var $this = $(this);<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src'));<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src').replace('/thumbs',''));<br />
});<br />
<br />
//starts the animation<br />
function start(){<br />
$im_loading.hide();<br />
//disperse the thumbs in a grid<br />
disperse();<br />
}<br />
<br />
//disperses the thumbs in a grid based on windows dimentions<br />
function disperse(){<br />
if(!flg_click) return;<br />
setflag();<br />
mode = 'grid';<br />
//center point for first thumb along the width of the window<br />
var spaces_w = $(window).width()/(per_line + 1);<br />
//center point for first thumb along the height of the window<br />
var spaces_h = $(window).height()/(per_col + 1);<br />
//let's disperse the thumbs equally on the page<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
//calculate left and top for each thumb,<br />
//considering how many we want per line<br />
var left = spaces_w*((i%per_line)+1) - $thumb.width()/2;<br />
var top = spaces_h*(Math.ceil((i+1)/per_line)) - $thumb.height()/2;<br />
//lets give a random degree to each thumb<br />
var r = Math.floor(Math.random()*41)-20;<br />
/*<br />
now we animate the thumb to its final positions;<br />
we also fade in its image, animate it to 115x115,<br />
and remove any background image of the thumb - this<br />
is not relevant for the first time we call disperse,<br />
but when changing from single to grid mode<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px'<br />
};<br />
else<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px',<br />
'rotate' : r + 'deg'<br />
};<br />
$thumb.stop()<br />
.animate(param,700,function(){<br />
if(i==nmb_thumbs-1)<br />
setflag();<br />
})<br />
.find('img')<br />
.fadeIn(700,function(){<br />
$thumb.css({<br />
'background-image' : 'none'<br />
});<br />
$(this).animate({<br />
'width' : '115px',<br />
'height' : '115px',<br />
'marginTop' : '5px',<br />
'marginLeft': '5px'<br />
},150);<br />
});<br />
});<br />
}<br />
<br />
//controls if we can click on the thumbs or not<br />
//if theres an animation in progress<br />
//we don't want the user to be able to click<br />
function setflag(){<br />
flg_click = !flg_click<br />
}<br />
<br />
/*<br />
when we click on a thumb, we want to merge them<br />
and show the full image that was clicked.<br />
we need to animate the thumbs positions in order<br />
to center the final image in the screen. The<br />
image itself is the background image that each thumb<br />
will have (different background positions)<br />
If we are currently seeing the single image,<br />
then we want to disperse the thumbs again,<br />
and with this, showing the thumbs images.<br />
*/<br />
$thumbs.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
var $this = $(this);<br />
current = $this.index();<br />
<br />
if(mode == 'grid'){<br />
mode = 'single';<br />
//the source of the full image<br />
var image_src = $this.find('img').attr('src').replace('/thumbs','');<br />
<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
var $image = $thumb.find('img');<br />
//first we animate the thumb image<br />
//to fill the thumbs dimentions<br />
$image.stop().animate({<br />
'width' : '100%',<br />
'height' : '100%',<br />
'marginTop' : '0px',<br />
'marginLeft': '0px'<br />
},150,function(){<br />
//calculate the dimentions of the full image<br />
var f_w = per_line * 125;<br />
var f_h = per_col * 125;<br />
var f_l = $(window).width()/2 - f_w/2<br />
var f_t = $(window).height()/2 - f_h/2<br />
/*<br />
set the background image for the thumb<br />
and animate the thumbs postions and rotation<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
else<br />
var param = {<br />
'rotate': '0deg',<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
$thumb.css({<br />
'background-image' : 'url('+image_src+')'<br />
}).stop()<br />
.animate(param,1200,function(){<br />
//insert navigation for the single mode<br />
if(i==nmb_thumbs-1){<br />
addNavigation();<br />
setflag();<br />
}<br />
});<br />
//fade out the thumb's image<br />
$image.fadeOut(700);<br />
});<br />
});<br />
}<br />
else{<br />
setflag();<br />
//remove navigation<br />
removeNavigation();<br />
//if we are on single mode then disperse the thumbs<br />
disperse();<br />
}<br />
});<br />
<br />
//removes the navigation buttons<br />
function removeNavigation(){<br />
$im_next.stop().animate({'right':'-50px'},300);<br />
$im_prev.stop().animate({'left':'-50px'},300);<br />
}<br />
<br />
//add the navigation buttons<br />
function addNavigation(){<br />
$im_next.stop().animate({'right':'0px'},300);<br />
$im_prev.stop().animate({'left':'0px'},300);<br />
}<br />
<br />
//User clicks next button (single mode)<br />
$im_next.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
++current;<br />
var $next_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($next_thumb.length>0){<br />
var image_src = $next_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
//we want to change each divs background image<br />
//on a different point of time<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
--current;<br />
return;<br />
}<br />
});<br />
<br />
//User clicks prev button (single mode)<br />
$im_prev.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
--current;<br />
var $prev_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($prev_thumb.length>0){<br />
var image_src = $prev_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
++current;<br />
return;<br />
}<br />
});<br />
<br />
//on windows resize call the disperse function<br />
$(window).smartresize(function(){<br />
removeNavigation()<br />
disperse();<br />
});<br />
<br />
//function to shuffle an array<br />
Array.shuffle = function( array ){<br />
for(<br />
var j, x, i = array.length; i;<br />
j = parseInt(Math.random() * i),<br />
x = array[--i], array[i] = array[j], array[j] = x<br />
);<br />
return array;<br />
};<br />
});<br />
</script><br />
<!-- 代码 结束 --><br />
</body><br />
</html></div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/gallery/sry
Team:Hong Kong CUHK/gallery/sry
2013-10-28T13:07:56Z
<p>Shengry0716: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" /><br />
<br />
<br />
<title>{title}photo gallery</title><br />
<br />
<br />
<br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery.transform-0.9.1.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>iGEM CUHK</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/slideshow.css&action=raw&ctype=text/css"><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/indexuse.css&action=raw&ctype=text/css"><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/search.css&action=raw&ctype=text/css"><br />
<br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery-2.0.2.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<br />
<script src="https://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.cycle.all.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
$('#slider').cycle({<br />
fx: 'scrollHorz', /* Change the fx 'fade' to 'scrollHorz' if you want*/<br />
speed: 'slow',<br />
timeout: 3000,<br />
next: '#next',<br />
prev: '#prev'<br />
});<br />
</script><br />
<script type="text/javascript"><br />
<!--<br />
function MM_showHideLayers() { //v9.0<br />
var i,p,v,obj,args=MM_showHideLayers.arguments;<br />
for (i=0; i<(args.length-2); i+=3) <br />
with (document) if (getElementById && ((obj=getElementById(args[i]))!=null)) { v=args[i+2];<br />
if (obj.style) { obj=obj.style; v=(v=='show')?'visible':(v=='hide')?'hidden':v; }<br />
obj.visibility=v; }<br />
}<br />
//--><br />
</script><br />
<br />
<style type="text/css"><br />
<!--<br />
#p-logo<br />
{visibility:hidden;}<br />
.firstHeading<br />
{visibility:hidden;}<br />
#top-section<br />
{height:2px;border:none;background-color:#660099;}<br />
body{background-color:#660099}<br />
#globalWrapper {<br />
width: 1210px;<br />
height:100%;<br />
}<br />
#search-controls {<br />
display:none;<br />
}<br />
#content<br />
{width: 1000px;border:none;background-color:#660099;padding:0px;margin-top:0px;line-height: 1.5em;color: black;}<br />
#footnote {background-color:#FFFF93;<br />
position:absolute;<br />
left:0;<br />
top:0%;<br />
width:100%;<br />
height:40px;<br />
z-index:4;<br />
text-align:center;<br />
}<br />
#archor {<br />
position:absolute;<br />
right:0%;<br />
top:0%;<br />
width:120px;<br />
height:20px;<br />
z-index:1;<br />
}<br />
body,td,th {<br />
color: #CCC;<br />
}<br />
#containerX2{<br />
position:relative;<br />
width:1100px;<br />
height:1600px;<br />
z-index:1;<br />
background-color: #FFF;<br />
}<br />
<br />
<br />
<br />
<br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
</head><br />
<body><br />
<!-- 代码 开始 --><br />
<br />
<br />
<div id="banner"><br />
<div id="containerX1" style="margin: 0px auto;"><br />
<div id="logocontainer"><br />
<a name="top" id="top"></a><br />
<div id="swLogo"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/4/4d/Logo_horizontal2.png" width="600" height="170" /></a></div><br />
<div id="iGEMlogo"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png" width="100%" height="100%" /></a></div><br />
<div id="CUHKlogo"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/6/64/Right_logo2.png" width="100%" height="100%" /></a></div><br />
</div> <br />
<br />
<div id="buttons"><br />
<div class="menubar" id="homeUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/e/ee/Bpurple_home.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="teamUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/team"><img src="https://static.igem.org/mediawiki/2013/d/d8/Bpurple_team.png" width="110" height="71" /></a></div><br />
<div class="menubar" id="projectUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/project"><img src="https://static.igem.org/mediawiki/2013/2/2d/Bpurple_project.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="biobrickUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/biobrick"><img src="https://static.igem.org/mediawiki/2013/b/b6/Bpurple_biobrick.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="hpUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/humanpractice"><img src="https://static.igem.org/mediawiki/2013/9/95/Bpurple_human_practice.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="modelUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/modelling"><img src="https://static.igem.org/mediawiki/2013/3/3f/Bpurple_modelling.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="safetyUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/safety"><img src="https://static.igem.org/mediawiki/2013/1/1e/Bpurple_safety.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="docUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/documentation"><img src="https://static.igem.org/mediawiki/2013/1/12/Bpurple_documentation.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="galleryUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/gallery"><img src="https://static.igem.org/mediawiki/2013/e/ed/Bpurple_gallery.png" width="110" height="71"/></a></div><br />
<div class="menubar" id="ackUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/acknowledgement"><img src="https://static.igem.org/mediawiki/2013/4/4b/Bpurple_ack.png" width="110" height="71"/></a></div><br />
</div><br />
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<div id="buttonsExt"><br />
<div class="buttonbar" id="one"></div><br />
<div class="buttonbar" id="teamEXT"><a href="member.html" class="linkEXT">Team Members</a> | <a href="ianda.html" class="linkEXT">Instructors and Advisors</a></div><br />
<div class="buttonbar" id="projectEXT"><a href="overview.html" class="linkEXT">Overview</a> | <a href="pah.html" class="linkEXT">PAH Degradtion</a> | <a href="vs.html" class="linkEXT">Voltage Switch</a></div><br />
<div class="buttonbar" id="biobrickEXT"><a href="parts.html" class="linkEXT">Parts</a> | <a href="construction.html" class="linkEXT">Construction Notes</a> | <a href="character.html" class="linkEXT">Characterizations</a></div><br />
<div class="buttonbar" id="fiv"></div><br />
<div class="buttonbar" id="six"></div><br />
<div class="buttonbar" id="docEXT"><a href="protocol.html" class="linkEXT">Protocols</a> | <a href="notebook.html" class="linkEXT">Notebooks</a></div><br />
<div class="buttonbar" id="eig"></div><br />
</div><br />
<br />
</div><br />
<div id="middle"><br />
<div id="containerX2" style="margin: 0px auto;"><br />
<div id="heading" style="margin: 0px auto;"><br />
<br />
</div></div> <br />
</div><br />
</div><br />
<br />
<h2>Photo Gallery</span></h2><br />
<br />
<div id="im_wrapper" class="im_wrapper"><br />
<div style="background-position:0px 0px;"><img src="images/thumbs/1.jpg" alt="" /></div><br />
<div style="background-position:-125px 0px;"><img src="images/thumbs/2.jpg" alt="" /></div><br />
<div style="background-position:-250px 0px;"><img src="images/thumbs/3.jpg" alt="" /></div><br />
<div style="background-position:-375px 0px;"><img src="images/thumbs/4.jpg" alt="" /></div><br />
<div style="background-position:-500px 0px;"><img src="images/thumbs/5.jpg" alt="" /></div><br />
<div style="background-position:-625px 0px;"><img src="images/thumbs/6.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -125px;"><img src="images/thumbs/7.jpg" alt="" /></div><br />
<div style="background-position:-125px -125px;"><img src="images/thumbs/8.jpg" alt="" /></div><br />
<div style="background-position:-250px -125px;"><img src="images/thumbs/9.jpg" alt="" /></div><br />
<div style="background-position:-375px -125px;"><img src="images/thumbs/10.jpg" alt="" /></div><br />
<div style="background-position:-500px -125px;"><img src="https://static.igem.org/mediawiki/igem.org/b/b8/135208_1416552698564358_849150045_o.jpg" alt="" /></div><br />
<div style="background-position:-625px -125px;"><img src="images/thumbs/12.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -250px;"><img src="images/thumbs/13.jpg" alt="" /></div><br />
<div style="background-position:-125px -250px;"><img src="images/thumbs/14.jpg" alt="" /></div><br />
<div style="background-position:-250px -250px;"><img src="images/thumbs/15.jpg" alt="" /></div><br />
<div style="background-position:-375px -250px;"><img src="images/thumbs/16.jpg" alt="" /></div><br />
<div style="background-position:-500px -250px;"><img src="images/thumbs/17.jpg" alt="" /></div><br />
<div style="background-position:-625px -250px;"><img src="images/thumbs/18.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -375px;"><img src="images/thumbs/19.jpg" alt="" /></div><br />
<div style="background-position:-125px -375px;"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/1008815_555403041163429_5406524_o.jpg" width="192" height="138"/" alt="" /></div><br />
<div style="background-position:-250px -375px;"><img src="images/thumbs/21.jpg" alt="" /></div><br />
<div style="background-position:-375px -375px;"><img src="images/thumbs/22.jpg" alt="" /></div><br />
<div style="background-position:-500px -375px;"><img src="images/thumbs/23.jpg" alt="" /></div><br />
<div style="background-position:-625px -375px;"><img src="images/thumbs/24.jpg" alt="" /></div><br />
</div><br />
<div id="im_loading" class="im_loading"></div><br />
<div id="im_next" class="im_next"></div><br />
<div id="im_prev" class="im_prev"></div><br />
<div><br />
<span class="reference"><br />
<a href="http://tympanus.net/codrops/2010/11/30/merging-image-boxes/">back to the Codrops tutorial</a><br />
<a href="http://www.flickr.com/photos/arcticpuppy/">Images by tibchris</a><br />
</span><br />
</div><br />
<br />
<!-- The JavaScript --><br />
<br />
<script type="text/javascript"><br />
//Paul Irish smartresize : http://paulirish.com/2009/throttled-smartresize-jquery-event-handler/<br />
(function($,sr){<br />
// debouncing function from John Hann<br />
// http://unscriptable.com/index.php/2009/03/20/debouncing-javascript-methods/<br />
var debounce = function (func, threshold, execAsap) {<br />
var timeout;<br />
return function debounced () {<br />
var obj = this, args = arguments;<br />
function delayed () {<br />
if (!execAsap)<br />
func.apply(obj, args);<br />
timeout = null;<br />
};<br />
if (timeout)<br />
clearTimeout(timeout);<br />
else if (execAsap)<br />
func.apply(obj, args);<br />
timeout = setTimeout(delayed, threshold || 100);<br />
};<br />
}<br />
//smartresize<br />
jQuery.fn[sr] = function(fn){ return fn ? this.bind('resize', debounce(fn)) : this.trigger(sr); };<br />
})(jQuery,'smartresize');<br />
</script><br />
<script type="text/javascript"> <br />
$(function() {<br />
//check if the user made the<br />
//mistake to open it with IE<br />
var ie = false;<br />
if ($.browser.msie)<br />
ie = true;<br />
//flag to control the click event<br />
var flg_click = true;<br />
//the wrapper<br />
var $im_wrapper = $('#im_wrapper');<br />
//the thumbs<br />
var $thumbs = $im_wrapper.children('div');<br />
//all the images<br />
var $thumb_imgs = $thumbs.find('img');<br />
//number of images<br />
var nmb_thumbs = $thumbs.length;<br />
//image loading status<br />
var $im_loading = $('#im_loading');<br />
//the next and previous buttons<br />
var $im_next = $('#im_next');<br />
var $im_prev = $('#im_prev');<br />
//number of thumbs per line<br />
var per_line = 6;<br />
//number of thumbs per column<br />
var per_col = Math.ceil(nmb_thumbs/per_line)<br />
//index of the current thumb<br />
var current = -1;<br />
//mode = grid | single<br />
var mode = 'grid';<br />
//an array with the positions of the thumbs<br />
//we will use it for the navigation in single mode<br />
var positionsArray = [];<br />
for(var i = 0; i < nmb_thumbs; ++i)<br />
positionsArray[i]=i;<br />
<br />
<br />
//preload all the images<br />
$im_loading.show();<br />
var loaded = 0;<br />
$thumb_imgs.each(function(){<br />
var $this = $(this);<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src'));<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src').replace('/thumbs',''));<br />
});<br />
<br />
//starts the animation<br />
function start(){<br />
$im_loading.hide();<br />
//disperse the thumbs in a grid<br />
disperse();<br />
}<br />
<br />
//disperses the thumbs in a grid based on windows dimentions<br />
function disperse(){<br />
if(!flg_click) return;<br />
setflag();<br />
mode = 'grid';<br />
//center point for first thumb along the width of the window<br />
var spaces_w = $(window).width()/(per_line + 1);<br />
//center point for first thumb along the height of the window<br />
var spaces_h = $(window).height()/(per_col + 1);<br />
//let's disperse the thumbs equally on the page<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
//calculate left and top for each thumb,<br />
//considering how many we want per line<br />
var left = spaces_w*((i%per_line)+1) - $thumb.width()/2;<br />
var top = spaces_h*(Math.ceil((i+1)/per_line)) - $thumb.height()/2;<br />
//lets give a random degree to each thumb<br />
var r = Math.floor(Math.random()*41)-20;<br />
/*<br />
now we animate the thumb to its final positions;<br />
we also fade in its image, animate it to 115x115,<br />
and remove any background image of the thumb - this<br />
is not relevant for the first time we call disperse,<br />
but when changing from single to grid mode<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px'<br />
};<br />
else<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px',<br />
'rotate' : r + 'deg'<br />
};<br />
$thumb.stop()<br />
.animate(param,700,function(){<br />
if(i==nmb_thumbs-1)<br />
setflag();<br />
})<br />
.find('img')<br />
.fadeIn(700,function(){<br />
$thumb.css({<br />
'background-image' : 'none'<br />
});<br />
$(this).animate({<br />
'width' : '115px',<br />
'height' : '115px',<br />
'marginTop' : '5px',<br />
'marginLeft': '5px'<br />
},150);<br />
});<br />
});<br />
}<br />
<br />
//controls if we can click on the thumbs or not<br />
//if theres an animation in progress<br />
//we don't want the user to be able to click<br />
function setflag(){<br />
flg_click = !flg_click<br />
}<br />
<br />
/*<br />
when we click on a thumb, we want to merge them<br />
and show the full image that was clicked.<br />
we need to animate the thumbs positions in order<br />
to center the final image in the screen. The<br />
image itself is the background image that each thumb<br />
will have (different background positions)<br />
If we are currently seeing the single image,<br />
then we want to disperse the thumbs again,<br />
and with this, showing the thumbs images.<br />
*/<br />
$thumbs.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
var $this = $(this);<br />
current = $this.index();<br />
<br />
if(mode == 'grid'){<br />
mode = 'single';<br />
//the source of the full image<br />
var image_src = $this.find('img').attr('src').replace('/thumbs','');<br />
<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
var $image = $thumb.find('img');<br />
//first we animate the thumb image<br />
//to fill the thumbs dimentions<br />
$image.stop().animate({<br />
'width' : '100%',<br />
'height' : '100%',<br />
'marginTop' : '0px',<br />
'marginLeft': '0px'<br />
},150,function(){<br />
//calculate the dimentions of the full image<br />
var f_w = per_line * 125;<br />
var f_h = per_col * 125;<br />
var f_l = $(window).width()/2 - f_w/2<br />
var f_t = $(window).height()/2 - f_h/2<br />
/*<br />
set the background image for the thumb<br />
and animate the thumbs postions and rotation<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
else<br />
var param = {<br />
'rotate': '0deg',<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
$thumb.css({<br />
'background-image' : 'url('+image_src+')'<br />
}).stop()<br />
.animate(param,1200,function(){<br />
//insert navigation for the single mode<br />
if(i==nmb_thumbs-1){<br />
addNavigation();<br />
setflag();<br />
}<br />
});<br />
//fade out the thumb's image<br />
$image.fadeOut(700);<br />
});<br />
});<br />
}<br />
else{<br />
setflag();<br />
//remove navigation<br />
removeNavigation();<br />
//if we are on single mode then disperse the thumbs<br />
disperse();<br />
}<br />
});<br />
<br />
//removes the navigation buttons<br />
function removeNavigation(){<br />
$im_next.stop().animate({'right':'-50px'},300);<br />
$im_prev.stop().animate({'left':'-50px'},300);<br />
}<br />
<br />
//add the navigation buttons<br />
function addNavigation(){<br />
$im_next.stop().animate({'right':'0px'},300);<br />
$im_prev.stop().animate({'left':'0px'},300);<br />
}<br />
<br />
//User clicks next button (single mode)<br />
$im_next.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
++current;<br />
var $next_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($next_thumb.length>0){<br />
var image_src = $next_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
//we want to change each divs background image<br />
//on a different point of time<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
--current;<br />
return;<br />
}<br />
});<br />
<br />
//User clicks prev button (single mode)<br />
$im_prev.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
--current;<br />
var $prev_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($prev_thumb.length>0){<br />
var image_src = $prev_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
++current;<br />
return;<br />
}<br />
});<br />
<br />
//on windows resize call the disperse function<br />
$(window).smartresize(function(){<br />
removeNavigation()<br />
disperse();<br />
});<br />
<br />
//function to shuffle an array<br />
Array.shuffle = function( array ){<br />
for(<br />
var j, x, i = array.length; i;<br />
j = parseInt(Math.random() * i),<br />
x = array[--i], array[i] = array[j], array[j] = x<br />
);<br />
return array;<br />
};<br />
});<br />
</script><br />
<!-- 代码 结束 --><br />
<br />
<br />
<div id="footer"><br />
<div id="containerX3" style="margin: 0px auto;"><br />
<div id="footnote"><br />
Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
<div id="backtop"><a href="#">TOP</a></div></div><br />
</div><br />
</div><br />
</div><br />
<br />
</div><br />
</body><br />
</html></div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/gallery/sry
Team:Hong Kong CUHK/gallery/sry
2013-10-28T12:42:16Z
<p>Shengry0716: Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> <title>{title}photo gallery</title> <link rel="styles..."</p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" /><br />
<br />
<br />
<title>{title}photo gallery</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/lrtk.css&action=raw&ctype=text/css"><br />
<br />
<script src="js/jquery.transform-0.9.1.min.js"></script><br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery.transform-0.9.1.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<br />
<br />
<br />
<br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
</head><br />
<body><br />
<!-- 代码 开始 --><br />
<h1>Photo Gallery</span></h1><br />
<br />
<div id="im_wrapper" class="im_wrapper"><br />
<div style="background-position:0px 0px;"><img src="images/thumbs/1.jpg" alt="" /></div><br />
<div style="background-position:-125px 0px;"><img src="images/thumbs/2.jpg" alt="" /></div><br />
<div style="background-position:-250px 0px;"><img src="images/thumbs/3.jpg" alt="" /></div><br />
<div style="background-position:-375px 0px;"><img src="images/thumbs/4.jpg" alt="" /></div><br />
<div style="background-position:-500px 0px;"><img src="images/thumbs/5.jpg" alt="" /></div><br />
<div style="background-position:-625px 0px;"><img src="images/thumbs/6.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -125px;"><img src="images/thumbs/7.jpg" alt="" /></div><br />
<div style="background-position:-125px -125px;"><img src="images/thumbs/8.jpg" alt="" /></div><br />
<div style="background-position:-250px -125px;"><img src="images/thumbs/9.jpg" alt="" /></div><br />
<div style="background-position:-375px -125px;"><img src="images/thumbs/10.jpg" alt="" /></div><br />
<div style="background-position:-500px -125px;"><img src="images/thumbs/11.jpg" alt="" /></div><br />
<div style="background-position:-625px -125px;"><img src="images/thumbs/12.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -250px;"><img src="images/thumbs/13.jpg" alt="" /></div><br />
<div style="background-position:-125px -250px;"><img src="images/thumbs/14.jpg" alt="" /></div><br />
<div style="background-position:-250px -250px;"><img src="images/thumbs/15.jpg" alt="" /></div><br />
<div style="background-position:-375px -250px;"><img src="images/thumbs/16.jpg" alt="" /></div><br />
<div style="background-position:-500px -250px;"><img src="images/thumbs/17.jpg" alt="" /></div><br />
<div style="background-position:-625px -250px;"><img src="images/thumbs/18.jpg" alt="" /></div><br />
<br />
<div style="background-position:0px -375px;"><img src="images/thumbs/19.jpg" alt="" /></div><br />
<div style="background-position:-125px -375px;"><img src="https://static.igem.org/mediawiki/igem.org/e/ec/1008815_555403041163429_5406524_o.jpg" width="192" height="138"/" alt="" /></div><br />
<div style="background-position:-250px -375px;"><img src="images/thumbs/21.jpg" alt="" /></div><br />
<div style="background-position:-375px -375px;"><img src="images/thumbs/22.jpg" alt="" /></div><br />
<div style="background-position:-500px -375px;"><img src="images/thumbs/23.jpg" alt="" /></div><br />
<div style="background-position:-625px -375px;"><img src="images/thumbs/24.jpg" alt="" /></div><br />
</div><br />
<div id="im_loading" class="im_loading"></div><br />
<div id="im_next" class="im_next"></div><br />
<div id="im_prev" class="im_prev"></div><br />
<div><br />
<span class="reference"><br />
<a href="http://tympanus.net/codrops/2010/11/30/merging-image-boxes/">back to the Codrops tutorial</a><br />
<a href="http://www.flickr.com/photos/arcticpuppy/">Images by tibchris</a><br />
</span><br />
</div><br />
<br />
<!-- The JavaScript --><br />
<br />
<script type="text/javascript"><br />
//Paul Irish smartresize : http://paulirish.com/2009/throttled-smartresize-jquery-event-handler/<br />
(function($,sr){<br />
// debouncing function from John Hann<br />
// http://unscriptable.com/index.php/2009/03/20/debouncing-javascript-methods/<br />
var debounce = function (func, threshold, execAsap) {<br />
var timeout;<br />
return function debounced () {<br />
var obj = this, args = arguments;<br />
function delayed () {<br />
if (!execAsap)<br />
func.apply(obj, args);<br />
timeout = null;<br />
};<br />
if (timeout)<br />
clearTimeout(timeout);<br />
else if (execAsap)<br />
func.apply(obj, args);<br />
timeout = setTimeout(delayed, threshold || 100);<br />
};<br />
}<br />
//smartresize<br />
jQuery.fn[sr] = function(fn){ return fn ? this.bind('resize', debounce(fn)) : this.trigger(sr); };<br />
})(jQuery,'smartresize');<br />
</script><br />
<script type="text/javascript"> <br />
$(function() {<br />
//check if the user made the<br />
//mistake to open it with IE<br />
var ie = false;<br />
if ($.browser.msie)<br />
ie = true;<br />
//flag to control the click event<br />
var flg_click = true;<br />
//the wrapper<br />
var $im_wrapper = $('#im_wrapper');<br />
//the thumbs<br />
var $thumbs = $im_wrapper.children('div');<br />
//all the images<br />
var $thumb_imgs = $thumbs.find('img');<br />
//number of images<br />
var nmb_thumbs = $thumbs.length;<br />
//image loading status<br />
var $im_loading = $('#im_loading');<br />
//the next and previous buttons<br />
var $im_next = $('#im_next');<br />
var $im_prev = $('#im_prev');<br />
//number of thumbs per line<br />
var per_line = 6;<br />
//number of thumbs per column<br />
var per_col = Math.ceil(nmb_thumbs/per_line)<br />
//index of the current thumb<br />
var current = -1;<br />
//mode = grid | single<br />
var mode = 'grid';<br />
//an array with the positions of the thumbs<br />
//we will use it for the navigation in single mode<br />
var positionsArray = [];<br />
for(var i = 0; i < nmb_thumbs; ++i)<br />
positionsArray[i]=i;<br />
<br />
<br />
//preload all the images<br />
$im_loading.show();<br />
var loaded = 0;<br />
$thumb_imgs.each(function(){<br />
var $this = $(this);<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src'));<br />
$('<img/>').load(function(){<br />
++loaded;<br />
if(loaded == nmb_thumbs*2)<br />
start();<br />
}).attr('src',$this.attr('src').replace('/thumbs',''));<br />
});<br />
<br />
//starts the animation<br />
function start(){<br />
$im_loading.hide();<br />
//disperse the thumbs in a grid<br />
disperse();<br />
}<br />
<br />
//disperses the thumbs in a grid based on windows dimentions<br />
function disperse(){<br />
if(!flg_click) return;<br />
setflag();<br />
mode = 'grid';<br />
//center point for first thumb along the width of the window<br />
var spaces_w = $(window).width()/(per_line + 1);<br />
//center point for first thumb along the height of the window<br />
var spaces_h = $(window).height()/(per_col + 1);<br />
//let's disperse the thumbs equally on the page<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
//calculate left and top for each thumb,<br />
//considering how many we want per line<br />
var left = spaces_w*((i%per_line)+1) - $thumb.width()/2;<br />
var top = spaces_h*(Math.ceil((i+1)/per_line)) - $thumb.height()/2;<br />
//lets give a random degree to each thumb<br />
var r = Math.floor(Math.random()*41)-20;<br />
/*<br />
now we animate the thumb to its final positions;<br />
we also fade in its image, animate it to 115x115,<br />
and remove any background image of the thumb - this<br />
is not relevant for the first time we call disperse,<br />
but when changing from single to grid mode<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px'<br />
};<br />
else<br />
var param = {<br />
'left' : left + 'px',<br />
'top' : top + 'px',<br />
'rotate' : r + 'deg'<br />
};<br />
$thumb.stop()<br />
.animate(param,700,function(){<br />
if(i==nmb_thumbs-1)<br />
setflag();<br />
})<br />
.find('img')<br />
.fadeIn(700,function(){<br />
$thumb.css({<br />
'background-image' : 'none'<br />
});<br />
$(this).animate({<br />
'width' : '115px',<br />
'height' : '115px',<br />
'marginTop' : '5px',<br />
'marginLeft': '5px'<br />
},150);<br />
});<br />
});<br />
}<br />
<br />
//controls if we can click on the thumbs or not<br />
//if theres an animation in progress<br />
//we don't want the user to be able to click<br />
function setflag(){<br />
flg_click = !flg_click<br />
}<br />
<br />
/*<br />
when we click on a thumb, we want to merge them<br />
and show the full image that was clicked.<br />
we need to animate the thumbs positions in order<br />
to center the final image in the screen. The<br />
image itself is the background image that each thumb<br />
will have (different background positions)<br />
If we are currently seeing the single image,<br />
then we want to disperse the thumbs again,<br />
and with this, showing the thumbs images.<br />
*/<br />
$thumbs.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
var $this = $(this);<br />
current = $this.index();<br />
<br />
if(mode == 'grid'){<br />
mode = 'single';<br />
//the source of the full image<br />
var image_src = $this.find('img').attr('src').replace('/thumbs','');<br />
<br />
$thumbs.each(function(i){<br />
var $thumb = $(this);<br />
var $image = $thumb.find('img');<br />
//first we animate the thumb image<br />
//to fill the thumbs dimentions<br />
$image.stop().animate({<br />
'width' : '100%',<br />
'height' : '100%',<br />
'marginTop' : '0px',<br />
'marginLeft': '0px'<br />
},150,function(){<br />
//calculate the dimentions of the full image<br />
var f_w = per_line * 125;<br />
var f_h = per_col * 125;<br />
var f_l = $(window).width()/2 - f_w/2<br />
var f_t = $(window).height()/2 - f_h/2<br />
/*<br />
set the background image for the thumb<br />
and animate the thumbs postions and rotation<br />
*/<br />
if(ie)<br />
var param = {<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
else<br />
var param = {<br />
'rotate': '0deg',<br />
'left' : f_l + (i%per_line)*125 + 'px',<br />
'top' : f_t + Math.floor(i/per_line)*125 + 'px'<br />
};<br />
$thumb.css({<br />
'background-image' : 'url('+image_src+')'<br />
}).stop()<br />
.animate(param,1200,function(){<br />
//insert navigation for the single mode<br />
if(i==nmb_thumbs-1){<br />
addNavigation();<br />
setflag();<br />
}<br />
});<br />
//fade out the thumb's image<br />
$image.fadeOut(700);<br />
});<br />
});<br />
}<br />
else{<br />
setflag();<br />
//remove navigation<br />
removeNavigation();<br />
//if we are on single mode then disperse the thumbs<br />
disperse();<br />
}<br />
});<br />
<br />
//removes the navigation buttons<br />
function removeNavigation(){<br />
$im_next.stop().animate({'right':'-50px'},300);<br />
$im_prev.stop().animate({'left':'-50px'},300);<br />
}<br />
<br />
//add the navigation buttons<br />
function addNavigation(){<br />
$im_next.stop().animate({'right':'0px'},300);<br />
$im_prev.stop().animate({'left':'0px'},300);<br />
}<br />
<br />
//User clicks next button (single mode)<br />
$im_next.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
<br />
++current;<br />
var $next_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($next_thumb.length>0){<br />
var image_src = $next_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
//we want to change each divs background image<br />
//on a different point of time<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
--current;<br />
return;<br />
}<br />
});<br />
<br />
//User clicks prev button (single mode)<br />
$im_prev.bind('click',function(){<br />
if(!flg_click) return;<br />
setflag();<br />
--current;<br />
var $prev_thumb = $im_wrapper.children('div:nth-child('+(current+1)+')');<br />
if($prev_thumb.length>0){<br />
var image_src = $prev_thumb.find('img').attr('src').replace('/thumbs','');<br />
var arr = Array.shuffle(positionsArray.slice(0));<br />
$thumbs.each(function(i){<br />
var t = $(this);<br />
setTimeout(function(){<br />
t.css({<br />
'background-image' : 'url('+image_src+')'<br />
});<br />
if(i == nmb_thumbs-1)<br />
setflag();<br />
},arr.shift()*20);<br />
});<br />
}<br />
else{<br />
setflag();<br />
++current;<br />
return;<br />
}<br />
});<br />
<br />
//on windows resize call the disperse function<br />
$(window).smartresize(function(){<br />
removeNavigation()<br />
disperse();<br />
});<br />
<br />
//function to shuffle an array<br />
Array.shuffle = function( array ){<br />
for(<br />
var j, x, i = array.length; i;<br />
j = parseInt(Math.random() * i),<br />
x = array[--i], array[i] = array[j], array[j] = x<br />
);<br />
return array;<br />
};<br />
});<br />
</script><br />
<!-- 代码 结束 --><br />
</body><br />
</html></div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/Templates/lrtk.css
Team:Hong Kong CUHK/Templates/lrtk.css
2013-10-28T12:37:34Z
<p>Shengry0716: Created page with "*{ margin:0; padding:0; } body{ background:#f0f0f0 url(../images/bg.jpg) repeat top left; font-family:Arial, Helvetica, sans-serif; font-size:12px; color: #555; } h1{ font..."</p>
<hr />
<div>*{<br />
margin:0;<br />
padding:0;<br />
}<br />
body{<br />
background:#f0f0f0 url(../images/bg.jpg) repeat top left;<br />
font-family:Arial, Helvetica, sans-serif;<br />
font-size:12px;<br />
color: #555;<br />
}<br />
h1{<br />
font-size:38px;<br />
margin:10px;<br />
}<br />
h1 span{<br />
font-size:20px;<br />
}<br />
.im_wrapper div{<br />
left:-500px;<br />
width:125px;<br />
height:125px;<br />
position:absolute;<br />
background-repeat:no-repeat;<br />
background-color:#fff;<br />
cursor:pointer;<br />
-moz-box-shadow:1px 1px 3px #000;<br />
-webkit-box-shadow:1px 1px 3px #000;<br />
box-shadow:1px 1px 3px #000;<br />
}<br />
.im_wrapper div img{<br />
float:left;<br />
width:115px;<br />
height:115px;<br />
margin:5px 0px 0px 5px;<br />
}<br />
.im_loading{<br />
display:none;<br />
position:fixed;<br />
top:50%;<br />
left:50%;<br />
margin:-35px 0px 0px -35px;<br />
background:#fff url(../images/loader.gif) no-repeat center center;<br />
width:70px;<br />
height:70px;<br />
z-index:9999;<br />
-moz-border-radius:10px;<br />
-webkit-border-radius:10px;<br />
border-radius:10px;<br />
-moz-box-shadow:1px 1px 3px #000;<br />
-webkit-box-shadow:1px 1px 3px #000;<br />
box-shadow:1px 1px 3px #000;<br />
opacity:0.7;<br />
filter:progid:DXImageTransform.Microsoft.Alpha(opacity=70);<br />
}<br />
.im_next,<br />
.im_prev{<br />
width:50px;<br />
height:50px;<br />
position:fixed;<br />
bottom:50%;<br />
margin-top:-25px;<br />
cursor:pointer;<br />
opacity:0.7;<br />
z-index:1000;<br />
-moz-box-shadow:0px 0px 3px #000;<br />
-webkit-box-shadow:0px 0px 3px #000;<br />
box-shadow:0px 0px 3px #000;<br />
-moz-border-radius:2px;<br />
-webkit-border-radius:2px;<br />
border-radius:2px;<br />
filter:progid:DXImageTransform.Microsoft.Alpha(opacity=70);<br />
}<br />
.im_next:hover,<br />
.im_prev:hover<br />
{<br />
opacity:0.9;<br />
}<br />
.im_next{<br />
background:#fff url(../images/next.png) no-repeat center center;<br />
right:-50px; /*10 to show*/<br />
}<br />
.im_prev{<br />
background:#fff url(../images/prev.png) no-repeat center center;<br />
left:-50px; /*10 to show*/<br />
}<br />
.description{position:fixed;right:10px; top:10px;font-size:12px;color:#888; }<br />
span.reference{position:fixed;left:10px;bottom:10px;font-size:12px; }<br />
span.reference a{color:#888;text-transform:uppercase;text-decoration:none;padding-right:20px;}<br />
span.reference a:hover{color:#444;}</div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.transform-0.9.1.min
Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min
2013-10-28T12:34:27Z
<p>Shengry0716: moved Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min to Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min.js</p>
<hr />
<div>#REDIRECT [[Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min.js]]</div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.transform-0.9.1.min.js
Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min.js
2013-10-28T12:34:27Z
<p>Shengry0716: moved Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min to Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min.js</p>
<hr />
<div>/*<br />
* jQuery 2d Transform v0.9.1<br />
* http://wiki.github.com/heygrady/transform/<br />
*<br />
* Copyright 2010, Grady Kuhnline<br />
* Dual licensed under the MIT or GPL Version 2 licenses.<br />
* http://jquery.org/license<br />
* <br />
* Date: Fri Nov 26 23:43:06 2010 -0800<br />
*/<br />
(function(f,g,j,b){var h=/progid:DXImageTransform\.Microsoft\.Matrix\(.*?\)/,c=/^([\+\-]=)?([\d+.\-]+)(.*)$/,q=/%/;var d=j.createElement("modernizr"),e=d.style;function n(s){return parseFloat(s)}function l(){var s={transformProperty:"",MozTransform:"-moz-",WebkitTransform:"-webkit-",OTransform:"-o-",msTransform:"-ms-"};for(var t in s){if(typeof e[t]!="undefined"){return s[t]}}return null}function r(){if(typeof(g.Modernizr)!=="undefined"){return Modernizr.csstransforms}var t=["transformProperty","WebkitTransform","MozTransform","OTransform","msTransform"];for(var s in t){if(e[t[s]]!==b){return true}}}var a=l(),i=a!==null?a+"transform":false,k=a!==null?a+"transform-origin":false;f.support.csstransforms=r();if(a=="-ms-"){i="msTransform";k="msTransformOrigin"}f.extend({transform:function(s){s.transform=this;this.$elem=f(s);this.applyingMatrix=false;this.matrix=null;this.height=null;this.width=null;this.outerHeight=null;this.outerWidth=null;this.boxSizingValue=null;this.boxSizingProperty=null;this.attr=null;this.transformProperty=i;this.transformOriginProperty=k}});f.extend(f.transform,{funcs:["matrix","origin","reflect","reflectX","reflectXY","reflectY","rotate","scale","scaleX","scaleY","skew","skewX","skewY","translate","translateX","translateY"]});f.fn.transform=function(s,t){return this.each(function(){var u=this.transform||new f.transform(this);if(s){u.exec(s,t)}})};f.transform.prototype={exec:function(s,t){t=f.extend(true,{forceMatrix:false,preserve:false},t);this.attr=null;if(t.preserve){s=f.extend(true,this.getAttrs(true,true),s)}else{s=f.extend(true,{},s)}this.setAttrs(s);if(f.support.csstransforms&&!t.forceMatrix){return this.execFuncs(s)}else{if(f.browser.msie||(f.support.csstransforms&&t.forceMatrix)){return this.execMatrix(s)}}return false},execFuncs:function(t){var s=[];for(var u in t){if(u=="origin"){this[u].apply(this,f.isArray(t[u])?t[u]:[t[u]])}else{if(f.inArray(u,f.transform.funcs)!==-1){s.push(this.createTransformFunc(u,t[u]))}}}this.$elem.css(i,s.join(" "));return true},execMatrix:function(z){var C,x,t;var F=this.$elem[0],B=this;function A(N,M){if(q.test(N)){return parseFloat(N)/100*B["safeOuter"+(M?"Height":"Width")]()}return o(F,N)}var s=/translate[X|Y]?/,u=[];for(var v in z){switch(f.type(z[v])){case"array":t=z[v];break;case"string":t=f.map(z[v].split(","),f.trim);break;default:t=[z[v]]}if(f.matrix[v]){if(f.cssAngle[v]){t=f.map(t,f.angle.toDegree)}else{if(!f.cssNumber[v]){t=f.map(t,A)}else{t=f.map(t,n)}}x=f.matrix[v].apply(this,t);if(s.test(v)){u.push(x)}else{C=C?C.x(x):x}}else{if(v=="origin"){this[v].apply(this,t)}}}C=C||f.matrix.identity();f.each(u,function(M,N){C=C.x(N)});var K=parseFloat(C.e(1,1).toFixed(6)),I=parseFloat(C.e(2,1).toFixed(6)),H=parseFloat(C.e(1,2).toFixed(6)),G=parseFloat(C.e(2,2).toFixed(6)),L=C.rows===3?parseFloat(C.e(1,3).toFixed(6)):0,J=C.rows===3?parseFloat(C.e(2,3).toFixed(6)):0;if(f.support.csstransforms&&a==="-moz-"){this.$elem.css(i,"matrix("+K+", "+I+", "+H+", "+G+", "+L+"px, "+J+"px)")}else{if(f.support.csstransforms){this.$elem.css(i,"matrix("+K+", "+I+", "+H+", "+G+", "+L+", "+J+")")}else{if(f.browser.msie){var w=", FilterType='nearest neighbor'";var D=this.$elem[0].style;var E="progid:DXImageTransform.Microsoft.Matrix(M11="+K+", M12="+H+", M21="+I+", M22="+G+", sizingMethod='auto expand'"+w+")";var y=D.filter||f.curCSS(this.$elem[0],"filter")||"";D.filter=h.test(y)?y.replace(h,E):y?y+" "+E:E;this.applyingMatrix=true;this.matrix=C;this.fixPosition(C,L,J);this.applyingMatrix=false;this.matrix=null}}}return true},origin:function(s,t){if(f.support.csstransforms){if(typeof t==="undefined"){this.$elem.css(k,s)}else{this.$elem.css(k,s+" "+t)}return true}switch(s){case"left":s="0";break;case"right":s="100%";break;case"center":case b:s="50%"}switch(t){case"top":t="0";break;case"bottom":t="100%";break;case"center":case b:t="50%"}this.setAttr("origin",[q.test(s)?s:o(this.$elem[0],s)+"px",q.test(t)?t:o(this.$elem[0],t)+"px"]);return true},createTransformFunc:function(t,u){if(t.substr(0,7)==="reflect"){var s=u?f.matrix[t]():f.matrix.identity();return"matrix("+s.e(1,1)+", "+s.e(2,1)+", "+s.e(1,2)+", "+s.e(2,2)+", 0, 0)"}if(t=="matrix"){if(a==="-moz-"&&u[4]){u[4]=u[4]?u[4]+"px":0;u[5]=u[5]?u[5]+"px":0}}return t+"("+(f.isArray(u)?u.join(", "):u)+")"},fixPosition:function(B,y,x,D,s){var w=new f.matrix.calc(B,this.safeOuterHeight(),this.safeOuterWidth()),C=this.getAttr("origin");var v=w.originOffset(new f.matrix.V2(q.test(C[0])?parseFloat(C[0])/100*w.outerWidth:parseFloat(C[0]),q.test(C[1])?parseFloat(C[1])/100*w.outerHeight:parseFloat(C[1])));var t=w.sides();var u=this.$elem.css("position");if(u=="static"){u="relative"}var A={top:0,left:0};var z={position:u,top:(v.top+x+t.top+A.top)+"px",left:(v.left+y+t.left+A.left)+"px",zoom:1};this.$elem.css(z)}};function o(s,u){var t=c.exec(f.trim(u));if(t[3]&&t[3]!=="px"){var w="paddingBottom",v=f.style(s,w);f.style(s,w,u);u=p(s,w);f.style(s,w,v);return u}return parseFloat(u)}function p(t,u){if(t[u]!=null&&(!t.style||t.style[u]==null)){return t[u]}var s=parseFloat(f.css(t,u));return s&&s>-10000?s:0}})(jQuery,this,this.document);(function(d,c,a,f){d.extend(d.transform.prototype,{safeOuterHeight:function(){return this.safeOuterLength("height")},safeOuterWidth:function(){return this.safeOuterLength("width")},safeOuterLength:function(l){var p="outer"+(l=="width"?"Width":"Height");if(!d.support.csstransforms&&d.browser.msie){l=l=="width"?"width":"height";if(this.applyingMatrix&&!this[p]&&this.matrix){var k=new d.matrix.calc(this.matrix,1,1),n=k.offset(),g=this.$elem[p]()/n[l];this[p]=g;return g}else{if(this.applyingMatrix&&this[p]){return this[p]}}var o={height:["top","bottom"],width:["left","right"]};var h=this.$elem[0],j=parseFloat(d.curCSS(h,l,true)),q=this.boxSizingProperty,i=this.boxSizingValue;if(!this.boxSizingProperty){q=this.boxSizingProperty=e()||"box-sizing";i=this.boxSizingValue=this.$elem.css(q)||"content-box"}if(this[p]&&this[l]==j){return this[p]}else{this[l]=j}if(q&&(i=="padding-box"||i=="content-box")){j+=parseFloat(d.curCSS(h,"padding-"+o[l][0],true))||0+parseFloat(d.curCSS(h,"padding-"+o[l][1],true))||0}if(q&&i=="content-box"){j+=parseFloat(d.curCSS(h,"border-"+o[l][0]+"-width",true))||0+parseFloat(d.curCSS(h,"border-"+o[l][1]+"-width",true))||0}this[p]=j;return j}return this.$elem[p]()}});var b=null;function e(){if(b){return b}var h={boxSizing:"box-sizing",MozBoxSizing:"-moz-box-sizing",WebkitBoxSizing:"-webkit-box-sizing",OBoxSizing:"-o-box-sizing"},g=a.body;for(var i in h){if(typeof g.style[i]!="undefined"){b=h[i];return b}}return null}})(jQuery,this,this.document);(function(g,f,b,h){var d=/([\w\-]*?)\((.*?)\)/g,a="data-transform",e=/\s/,c=/,\s?/;g.extend(g.transform.prototype,{setAttrs:function(i){var j="",l;for(var k in i){l=i[k];if(g.isArray(l)){l=l.join(", ")}j+=" "+k+"("+l+")"}this.attr=g.trim(j);this.$elem.attr(a,this.attr)},setAttr:function(k,l){if(g.isArray(l)){l=l.join(", ")}var j=this.attr||this.$elem.attr(a);if(!j||j.indexOf(k)==-1){this.attr=g.trim(j+" "+k+"("+l+")");this.$elem.attr(a,this.attr)}else{var i=[],n;d.lastIndex=0;while(n=d.exec(j)){if(k==n[1]){i.push(k+"("+l+")")}else{i.push(n[0])}}this.attr=i.join(" ");this.$elem.attr(a,this.attr)}},getAttrs:function(){var j=this.attr||this.$elem.attr(a);if(!j){return{}}var i={},l,k;d.lastIndex=0;while((l=d.exec(j))!==null){if(l){k=l[2].split(c);i[l[1]]=k.length==1?k[0]:k}}return i},getAttr:function(j){var i=this.getAttrs();if(typeof i[j]!=="undefined"){return i[j]}if(j==="origin"&&g.support.csstransforms){return this.$elem.css(this.transformOriginProperty).split(e)}else{if(j==="origin"){return["50%","50%"]}}return g.cssDefault[j]||0}});if(typeof(g.cssAngle)=="undefined"){g.cssAngle={}}g.extend(g.cssAngle,{rotate:true,skew:true,skewX:true,skewY:true});if(typeof(g.cssDefault)=="undefined"){g.cssDefault={}}g.extend(g.cssDefault,{scale:[1,1],scaleX:1,scaleY:1,matrix:[1,0,0,1,0,0],origin:["50%","50%"],reflect:[1,0,0,1,0,0],reflectX:[1,0,0,1,0,0],reflectXY:[1,0,0,1,0,0],reflectY:[1,0,0,1,0,0]});if(typeof(g.cssMultipleValues)=="undefined"){g.cssMultipleValues={}}g.extend(g.cssMultipleValues,{matrix:6,origin:{length:2,duplicate:true},reflect:6,reflectX:6,reflectXY:6,reflectY:6,scale:{length:2,duplicate:true},skew:2,translate:2});g.extend(g.cssNumber,{matrix:true,reflect:true,reflectX:true,reflectXY:true,reflectY:true,scale:true,scaleX:true,scaleY:true});g.each(g.transform.funcs,function(j,k){g.cssHooks[k]={set:function(n,o){var l=n.transform||new g.transform(n),i={};i[k]=o;l.exec(i,{preserve:true})},get:function(n,l){var i=n.transform||new g.transform(n);return i.getAttr(k)}}});g.each(["reflect","reflectX","reflectXY","reflectY"],function(j,k){g.cssHooks[k].get=function(n,l){var i=n.transform||new g.transform(n);return i.getAttr("matrix")||g.cssDefault[k]}})})(jQuery,this,this.document);(function(e,g,h,c){var d=/^([+\-]=)?([\d+.\-]+)(.*)$/;var a=e.fn.animate;e.fn.animate=function(p,l,o,n){var k=e.speed(l,o,n),j=e.cssMultipleValues;k.complete=k.old;if(!e.isEmptyObject(p)){if(typeof k.original==="undefined"){k.original={}}e.each(p,function(s,u){if(j[s]||e.cssAngle[s]||(!e.cssNumber[s]&&e.inArray(s,e.transform.funcs)!==-1)){var t=null;if(jQuery.isArray(p[s])){var r=1,q=u.length;if(j[s]){r=(typeof j[s].length==="undefined"?j[s]:j[s].length)}if(q>r||(q<r&&q==2)||(q==2&&r==2&&isNaN(parseFloat(u[q-1])))){t=u[q-1];u.splice(q-1,1)}}k.original[s]=u.toString();p[s]=parseFloat(u)}})}return a.apply(this,[arguments[0],k])};var b="paddingBottom";function i(k,l){if(k[l]!=null&&(!k.style||k.style[l]==null)){}var j=parseFloat(e.css(k,l));return j&&j>-10000?j:0}var f=e.fx.prototype.custom;e.fx.prototype.custom=function(u,v,w){var y=e.cssMultipleValues[this.prop],p=e.cssAngle[this.prop];if(y||(!e.cssNumber[this.prop]&&e.inArray(this.prop,e.transform.funcs)!==-1)){this.values=[];if(!y){y=1}var x=this.options.original[this.prop],t=e(this.elem).css(this.prop),j=e.cssDefault[this.prop]||0;if(!e.isArray(t)){t=[t]}if(!e.isArray(x)){if(e.type(x)==="string"){x=x.split(",")}else{x=[x]}}var l=y.length||y,s=0;while(x.length<l){x.push(y.duplicate?x[0]:j[s]||0);s++}var k,r,q,o=this,n=o.elem.transform;orig=e.style(o.elem,b);e.each(x,function(z,A){if(t[z]){k=t[z]}else{if(j[z]&&!y.duplicate){k=j[z]}else{if(y.duplicate){k=t[0]}else{k=0}}}if(p){k=e.angle.toDegree(k)}else{if(!e.cssNumber[o.prop]){r=d.exec(e.trim(k));if(r[3]&&r[3]!=="px"){if(r[3]==="%"){k=parseFloat(r[2])/100*n["safeOuter"+(z?"Height":"Width")]()}else{e.style(o.elem,b,k);k=i(o.elem,b);e.style(o.elem,b,orig)}}}}k=parseFloat(k);r=d.exec(e.trim(A));if(r){q=parseFloat(r[2]);w=r[3]||"px";if(p){q=e.angle.toDegree(q+w);w="deg"}else{if(!e.cssNumber[o.prop]&&w==="%"){k=(k/n["safeOuter"+(z?"Height":"Width")]())*100}else{if(!e.cssNumber[o.prop]&&w!=="px"){e.style(o.elem,b,(q||1)+w);k=((q||1)/i(o.elem,b))*k;e.style(o.elem,b,orig)}}}if(r[1]){q=((r[1]==="-="?-1:1)*q)+k}}else{q=A;w=""}o.values.push({start:k,end:q,unit:w})})}return f.apply(this,arguments)};e.fx.multipleValueStep={_default:function(j){e.each(j.values,function(k,l){j.values[k].now=l.start+((l.end-l.start)*j.pos)})}};e.each(["matrix","reflect","reflectX","reflectXY","reflectY"],function(j,k){e.fx.multipleValueStep[k]=function(n){var p=n.decomposed,l=e.matrix;m=l.identity();p.now={};e.each(p.start,function(q){p.now[q]=parseFloat(p.start[q])+((parseFloat(p.end[q])-parseFloat(p.start[q]))*n.pos);if(((q==="scaleX"||q==="scaleY")&&p.now[q]===1)||((q!=="scaleX"||q!=="scaleY")&&p.now[q]===0)){return true}m=m.x(l[q](p.now[q]))});var o;e.each(n.values,function(q){switch(q){case 0:o=parseFloat(m.e(1,1).toFixed(6));break;case 1:o=parseFloat(m.e(1,2).toFixed(6));break;case 2:o=parseFloat(m.e(2,1).toFixed(6));break;case 3:o=parseFloat(m.e(2,2).toFixed(6));break;case 4:o=parseFloat(m.e(3,1).toFixed(6));break;case 5:o=parseFloat(m.e(3,2).toFixed(6));break}n.values[q].now=o})}});e.each(e.transform.funcs,function(j,k){e.fx.step[k]=function(o){var n=o.elem.transform||new e.transform(o.elem),l={};if(e.cssMultipleValues[k]||(!e.cssNumber[k]&&e.inArray(k,e.transform.funcs)!==-1)){(e.fx.multipleValueStep[o.prop]||e.fx.multipleValueStep._default)(o);l[o.prop]=[];e.each(o.values,function(p,q){l[o.prop].push(q.now+(e.cssNumber[o.prop]?"":q.unit))})}else{l[o.prop]=o.now+(e.cssNumber[o.prop]?"":o.unit)}n.exec(l,{preserve:true})}});e.each(["matrix","reflect","reflectX","reflectXY","reflectY"],function(j,k){e.fx.step[k]=function(q){var p=q.elem.transform||new e.transform(q.elem),o={};if(!q.initialized){q.initialized=true;if(k!=="matrix"){var n=e.matrix[k]().elements;var r;e.each(q.values,function(s){switch(s){case 0:r=n[0];break;case 1:r=n[2];break;case 2:r=n[1];break;case 3:r=n[3];break;default:r=0}q.values[s].end=r})}q.decomposed={};var l=q.values;q.decomposed.start=e.matrix.matrix(l[0].start,l[1].start,l[2].start,l[3].start,l[4].start,l[5].start).decompose();q.decomposed.end=e.matrix.matrix(l[0].end,l[1].end,l[2].end,l[3].end,l[4].end,l[5].end).decompose()}(e.fx.multipleValueStep[q.prop]||e.fx.multipleValueStep._default)(q);o.matrix=[];e.each(q.values,function(s,t){o.matrix.push(t.now)});p.exec(o,{preserve:true})}})})(jQuery,this,this.document);(function(g,h,j,c){var d=180/Math.PI;var k=200/Math.PI;var f=Math.PI/180;var e=2/1.8;var i=0.9;var a=Math.PI/200;var b=/^([+\-]=)?([\d+.\-]+)(.*)$/;g.extend({angle:{runit:/(deg|g?rad)/,radianToDegree:function(l){return l*d},radianToGrad:function(l){return l*k},degreeToRadian:function(l){return l*f},degreeToGrad:function(l){return l*e},gradToDegree:function(l){return l*i},gradToRadian:function(l){return l*a},toDegree:function(n){var l=b.exec(n);if(l){n=parseFloat(l[2]);switch(l[3]||"deg"){case"grad":n=g.angle.gradToDegree(n);break;case"rad":n=g.angle.radianToDegree(n);break}return n}return 0}}})})(jQuery,this,this.document);(function(e,d,b,f){if(typeof(e.matrix)=="undefined"){e.extend({matrix:{}})}e.extend(e.matrix,{V2:function(g,h){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,2)}else{this.elements=[g,h]}this.length=2},V3:function(g,i,h){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,3)}else{this.elements=[g,i,h]}this.length=3},M2x2:function(h,g,j,i){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,4)}else{this.elements=Array.prototype.slice.call(arguments).slice(0,4)}this.rows=2;this.cols=2},M3x3:function(l,k,j,i,h,g,p,o,n){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,9)}else{this.elements=Array.prototype.slice.call(arguments).slice(0,9)}this.rows=3;this.cols=3}});var c={e:function(j,g){var h=this.rows,i=this.cols;if(j>h||g>h||j<1||g<1){return 0}return this.elements[(j-1)*i+g-1]},decompose:function(){var u=this.e(1,1),s=this.e(2,1),p=this.e(1,2),o=this.e(2,2),n=this.e(3,1),l=this.e(3,2);if(Math.abs(u*o-s*p)<0.01){return{rotate:0+"deg",skewX:0+"deg",scaleX:1,scaleY:1,translateX:0+"px",translateY:0+"px"}}var j=n,i=l;var t=Math.sqrt(u*u+s*s);u=u/t;s=s/t;var h=u*p+s*o;p-=u*h;o-=s*h;var q=Math.sqrt(p*p+o*o);p=p/q;o=o/q;h=h/q;if((u*o-s*p)<0){u=-u;s=-s;t=-t}var v=e.angle.radianToDegree;var g=v(Math.atan2(s,u));h=v(Math.atan(h));return{rotate:g+"deg",skewX:h+"deg",scaleX:t,scaleY:q,translateX:j+"px",translateY:i+"px"}}};e.extend(e.matrix.M2x2.prototype,c,{toM3x3:function(){var g=this.elements;return new e.matrix.M3x3(g[0],g[1],0,g[2],g[3],0,0,0,1)},x:function(i){var j=typeof(i.rows)==="undefined";if(!j&&i.rows==3){return this.toM3x3().x(i)}var h=this.elements,g=i.elements;if(j&&g.length==2){return new e.matrix.V2(h[0]*g[0]+h[1]*g[1],h[2]*g[0]+h[3]*g[1])}else{if(g.length==h.length){return new e.matrix.M2x2(h[0]*g[0]+h[1]*g[2],h[0]*g[1]+h[1]*g[3],h[2]*g[0]+h[3]*g[2],h[2]*g[1]+h[3]*g[3])}}return false},inverse:function(){var h=1/this.determinant(),g=this.elements;return new e.matrix.M2x2(h*g[3],h*-g[1],h*-g[2],h*g[0])},determinant:function(){var g=this.elements;return g[0]*g[3]-g[1]*g[2]}});e.extend(e.matrix.M3x3.prototype,c,{x:function(i){var j=typeof(i.rows)==="undefined";if(!j&&i.rows<3){i=i.toM3x3()}var h=this.elements,g=i.elements;if(j&&g.length==3){return new e.matrix.V3(h[0]*g[0]+h[1]*g[1]+h[2]*g[2],h[3]*g[0]+h[4]*g[1]+h[5]*g[2],h[6]*g[0]+h[7]*g[1]+h[8]*g[2])}else{if(g.length==h.length){return new e.matrix.M3x3(h[0]*g[0]+h[1]*g[3]+h[2]*g[6],h[0]*g[1]+h[1]*g[4]+h[2]*g[7],h[0]*g[2]+h[1]*g[5]+h[2]*g[8],h[3]*g[0]+h[4]*g[3]+h[5]*g[6],h[3]*g[1]+h[4]*g[4]+h[5]*g[7],h[3]*g[2]+h[4]*g[5]+h[5]*g[8],h[6]*g[0]+h[7]*g[3]+h[8]*g[6],h[6]*g[1]+h[7]*g[4]+h[8]*g[7],h[6]*g[2]+h[7]*g[5]+h[8]*g[8])}}return false},inverse:function(){var h=1/this.determinant(),g=this.elements;return new e.matrix.M3x3(h*(g[8]*g[4]-g[7]*g[5]),h*(-(g[8]*g[1]-g[7]*g[2])),h*(g[5]*g[1]-g[4]*g[2]),h*(-(g[8]*g[3]-g[6]*g[5])),h*(g[8]*g[0]-g[6]*g[2]),h*(-(g[5]*g[0]-g[3]*g[2])),h*(g[7]*g[3]-g[6]*g[4]),h*(-(g[7]*g[0]-g[6]*g[1])),h*(g[4]*g[0]-g[3]*g[1]))},determinant:function(){var g=this.elements;return g[0]*(g[8]*g[4]-g[7]*g[5])-g[3]*(g[8]*g[1]-g[7]*g[2])+g[6]*(g[5]*g[1]-g[4]*g[2])}});var a={e:function(g){return this.elements[g-1]}};e.extend(e.matrix.V2.prototype,a);e.extend(e.matrix.V3.prototype,a)})(jQuery,this,this.document);(function(c,b,a,d){if(typeof(c.matrix)=="undefined"){c.extend({matrix:{}})}c.extend(c.matrix,{calc:function(e,f,g){this.matrix=e;this.outerHeight=f;this.outerWidth=g}});c.matrix.calc.prototype={coord:function(e,i,h){h=typeof(h)!=="undefined"?h:0;var g=this.matrix,f;switch(g.rows){case 2:f=g.x(new c.matrix.V2(e,i));break;case 3:f=g.x(new c.matrix.V3(e,i,h));break}return f},corners:function(e,h){var f=!(typeof(e)!=="undefined"||typeof(h)!=="undefined"),g;if(!this.c||!f){h=h||this.outerHeight;e=e||this.outerWidth;g={tl:this.coord(0,0),bl:this.coord(0,h),tr:this.coord(e,0),br:this.coord(e,h)}}else{g=this.c}if(f){this.c=g}return g},sides:function(e){var f=e||this.corners();return{top:Math.min(f.tl.e(2),f.tr.e(2),f.br.e(2),f.bl.e(2)),bottom:Math.max(f.tl.e(2),f.tr.e(2),f.br.e(2),f.bl.e(2)),left:Math.min(f.tl.e(1),f.tr.e(1),f.br.e(1),f.bl.e(1)),right:Math.max(f.tl.e(1),f.tr.e(1),f.br.e(1),f.bl.e(1))}},offset:function(e){var f=this.sides(e);return{height:Math.abs(f.bottom-f.top),width:Math.abs(f.right-f.left)}},area:function(e){var h=e||this.corners();var g={x:h.tr.e(1)-h.tl.e(1)+h.br.e(1)-h.bl.e(1),y:h.tr.e(2)-h.tl.e(2)+h.br.e(2)-h.bl.e(2)},f={x:h.bl.e(1)-h.tl.e(1)+h.br.e(1)-h.tr.e(1),y:h.bl.e(2)-h.tl.e(2)+h.br.e(2)-h.tr.e(2)};return 0.25*Math.abs(g.e(1)*f.e(2)-g.e(2)*f.e(1))},nonAffinity:function(){var f=this.sides(),g=f.top-f.bottom,e=f.left-f.right;return parseFloat(parseFloat(Math.abs((Math.pow(g,2)+Math.pow(e,2))/(f.top*f.bottom+f.left*f.right))).toFixed(8))},originOffset:function(h,g){h=h?h:new c.matrix.V2(this.outerWidth*0.5,this.outerHeight*0.5);g=g?g:new c.matrix.V2(0,0);var e=this.coord(h.e(1),h.e(2));var f=this.coord(g.e(1),g.e(2));return{top:(f.e(2)-g.e(2))-(e.e(2)-h.e(2)),left:(f.e(1)-g.e(1))-(e.e(1)-h.e(1))}}}})(jQuery,this,this.document);(function(c,b,a,d){if(typeof(c.matrix)=="undefined"){c.extend({matrix:{}})}c.extend(c.matrix,{identity:function(g){g=g||2;var h=g*g,j=new Array(h),f=g+1;for(var e=0;e<h;e++){j[e]=(e%f)===0?1:0}return new c.matrix["M"+g+"x"+g](j)},matrix:function(){var e=Array.prototype.slice.call(arguments);switch(arguments.length){case 4:return new c.matrix.M2x2(e[0],e[2],e[1],e[3]);case 6:return new c.matrix.M3x3(e[0],e[2],e[4],e[1],e[3],e[5],0,0,1)}},reflect:function(){return new c.matrix.M2x2(-1,0,0,-1)},reflectX:function(){return new c.matrix.M2x2(1,0,0,-1)},reflectXY:function(){return new c.matrix.M2x2(0,1,1,0)},reflectY:function(){return new c.matrix.M2x2(-1,0,0,1)},rotate:function(i){var f=c.angle.degreeToRadian(i),h=Math.cos(f),j=Math.sin(f);var g=h,e=j,l=-j,k=h;return new c.matrix.M2x2(g,l,e,k)},scale:function(f,e){f=f||f===0?f:1;e=e||e===0?e:f;return new c.matrix.M2x2(f,0,0,e)},scaleX:function(e){return c.matrix.scale(e,1)},scaleY:function(e){return c.matrix.scale(1,e)},skew:function(h,f){h=h||0;f=f||0;var i=c.angle.degreeToRadian(h),g=c.angle.degreeToRadian(f),e=Math.tan(i),j=Math.tan(g);return new c.matrix.M2x2(1,e,j,1)},skewX:function(e){return c.matrix.skew(e)},skewY:function(e){return c.matrix.skew(0,e)},translate:function(f,e){f=f||0;e=e||0;return new c.matrix.M3x3(1,0,f,0,1,e,0,0,1)},translateX:function(e){return c.matrix.translate(e)},translateY:function(e){return c.matrix.translate(0,e)}})})(jQuery,this,this.document);</div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.transform-0.9.1.min.js
Team:Hong Kong CUHK/Templates/jquery.transform-0.9.1.min.js
2013-10-28T12:32:18Z
<p>Shengry0716: Created page with "/* * jQuery 2d Transform v0.9.1 * http://wiki.github.com/heygrady/transform/ * * Copyright 2010, Grady Kuhnline * Dual licensed under the MIT or GPL Version 2 licenses. * h..."</p>
<hr />
<div>/*<br />
* jQuery 2d Transform v0.9.1<br />
* http://wiki.github.com/heygrady/transform/<br />
*<br />
* Copyright 2010, Grady Kuhnline<br />
* Dual licensed under the MIT or GPL Version 2 licenses.<br />
* http://jquery.org/license<br />
* <br />
* Date: Fri Nov 26 23:43:06 2010 -0800<br />
*/<br />
(function(f,g,j,b){var h=/progid:DXImageTransform\.Microsoft\.Matrix\(.*?\)/,c=/^([\+\-]=)?([\d+.\-]+)(.*)$/,q=/%/;var d=j.createElement("modernizr"),e=d.style;function n(s){return parseFloat(s)}function l(){var s={transformProperty:"",MozTransform:"-moz-",WebkitTransform:"-webkit-",OTransform:"-o-",msTransform:"-ms-"};for(var t in s){if(typeof e[t]!="undefined"){return s[t]}}return null}function r(){if(typeof(g.Modernizr)!=="undefined"){return Modernizr.csstransforms}var t=["transformProperty","WebkitTransform","MozTransform","OTransform","msTransform"];for(var s in t){if(e[t[s]]!==b){return true}}}var a=l(),i=a!==null?a+"transform":false,k=a!==null?a+"transform-origin":false;f.support.csstransforms=r();if(a=="-ms-"){i="msTransform";k="msTransformOrigin"}f.extend({transform:function(s){s.transform=this;this.$elem=f(s);this.applyingMatrix=false;this.matrix=null;this.height=null;this.width=null;this.outerHeight=null;this.outerWidth=null;this.boxSizingValue=null;this.boxSizingProperty=null;this.attr=null;this.transformProperty=i;this.transformOriginProperty=k}});f.extend(f.transform,{funcs:["matrix","origin","reflect","reflectX","reflectXY","reflectY","rotate","scale","scaleX","scaleY","skew","skewX","skewY","translate","translateX","translateY"]});f.fn.transform=function(s,t){return this.each(function(){var u=this.transform||new f.transform(this);if(s){u.exec(s,t)}})};f.transform.prototype={exec:function(s,t){t=f.extend(true,{forceMatrix:false,preserve:false},t);this.attr=null;if(t.preserve){s=f.extend(true,this.getAttrs(true,true),s)}else{s=f.extend(true,{},s)}this.setAttrs(s);if(f.support.csstransforms&&!t.forceMatrix){return this.execFuncs(s)}else{if(f.browser.msie||(f.support.csstransforms&&t.forceMatrix)){return this.execMatrix(s)}}return false},execFuncs:function(t){var s=[];for(var u in t){if(u=="origin"){this[u].apply(this,f.isArray(t[u])?t[u]:[t[u]])}else{if(f.inArray(u,f.transform.funcs)!==-1){s.push(this.createTransformFunc(u,t[u]))}}}this.$elem.css(i,s.join(" "));return true},execMatrix:function(z){var C,x,t;var F=this.$elem[0],B=this;function A(N,M){if(q.test(N)){return parseFloat(N)/100*B["safeOuter"+(M?"Height":"Width")]()}return o(F,N)}var s=/translate[X|Y]?/,u=[];for(var v in z){switch(f.type(z[v])){case"array":t=z[v];break;case"string":t=f.map(z[v].split(","),f.trim);break;default:t=[z[v]]}if(f.matrix[v]){if(f.cssAngle[v]){t=f.map(t,f.angle.toDegree)}else{if(!f.cssNumber[v]){t=f.map(t,A)}else{t=f.map(t,n)}}x=f.matrix[v].apply(this,t);if(s.test(v)){u.push(x)}else{C=C?C.x(x):x}}else{if(v=="origin"){this[v].apply(this,t)}}}C=C||f.matrix.identity();f.each(u,function(M,N){C=C.x(N)});var K=parseFloat(C.e(1,1).toFixed(6)),I=parseFloat(C.e(2,1).toFixed(6)),H=parseFloat(C.e(1,2).toFixed(6)),G=parseFloat(C.e(2,2).toFixed(6)),L=C.rows===3?parseFloat(C.e(1,3).toFixed(6)):0,J=C.rows===3?parseFloat(C.e(2,3).toFixed(6)):0;if(f.support.csstransforms&&a==="-moz-"){this.$elem.css(i,"matrix("+K+", "+I+", "+H+", "+G+", "+L+"px, "+J+"px)")}else{if(f.support.csstransforms){this.$elem.css(i,"matrix("+K+", "+I+", "+H+", "+G+", "+L+", "+J+")")}else{if(f.browser.msie){var w=", FilterType='nearest neighbor'";var D=this.$elem[0].style;var E="progid:DXImageTransform.Microsoft.Matrix(M11="+K+", M12="+H+", M21="+I+", M22="+G+", sizingMethod='auto expand'"+w+")";var y=D.filter||f.curCSS(this.$elem[0],"filter")||"";D.filter=h.test(y)?y.replace(h,E):y?y+" "+E:E;this.applyingMatrix=true;this.matrix=C;this.fixPosition(C,L,J);this.applyingMatrix=false;this.matrix=null}}}return true},origin:function(s,t){if(f.support.csstransforms){if(typeof t==="undefined"){this.$elem.css(k,s)}else{this.$elem.css(k,s+" "+t)}return true}switch(s){case"left":s="0";break;case"right":s="100%";break;case"center":case b:s="50%"}switch(t){case"top":t="0";break;case"bottom":t="100%";break;case"center":case b:t="50%"}this.setAttr("origin",[q.test(s)?s:o(this.$elem[0],s)+"px",q.test(t)?t:o(this.$elem[0],t)+"px"]);return true},createTransformFunc:function(t,u){if(t.substr(0,7)==="reflect"){var s=u?f.matrix[t]():f.matrix.identity();return"matrix("+s.e(1,1)+", "+s.e(2,1)+", "+s.e(1,2)+", "+s.e(2,2)+", 0, 0)"}if(t=="matrix"){if(a==="-moz-"&&u[4]){u[4]=u[4]?u[4]+"px":0;u[5]=u[5]?u[5]+"px":0}}return t+"("+(f.isArray(u)?u.join(", "):u)+")"},fixPosition:function(B,y,x,D,s){var w=new f.matrix.calc(B,this.safeOuterHeight(),this.safeOuterWidth()),C=this.getAttr("origin");var v=w.originOffset(new f.matrix.V2(q.test(C[0])?parseFloat(C[0])/100*w.outerWidth:parseFloat(C[0]),q.test(C[1])?parseFloat(C[1])/100*w.outerHeight:parseFloat(C[1])));var t=w.sides();var u=this.$elem.css("position");if(u=="static"){u="relative"}var A={top:0,left:0};var z={position:u,top:(v.top+x+t.top+A.top)+"px",left:(v.left+y+t.left+A.left)+"px",zoom:1};this.$elem.css(z)}};function o(s,u){var t=c.exec(f.trim(u));if(t[3]&&t[3]!=="px"){var w="paddingBottom",v=f.style(s,w);f.style(s,w,u);u=p(s,w);f.style(s,w,v);return u}return parseFloat(u)}function p(t,u){if(t[u]!=null&&(!t.style||t.style[u]==null)){return t[u]}var s=parseFloat(f.css(t,u));return s&&s>-10000?s:0}})(jQuery,this,this.document);(function(d,c,a,f){d.extend(d.transform.prototype,{safeOuterHeight:function(){return this.safeOuterLength("height")},safeOuterWidth:function(){return this.safeOuterLength("width")},safeOuterLength:function(l){var p="outer"+(l=="width"?"Width":"Height");if(!d.support.csstransforms&&d.browser.msie){l=l=="width"?"width":"height";if(this.applyingMatrix&&!this[p]&&this.matrix){var k=new d.matrix.calc(this.matrix,1,1),n=k.offset(),g=this.$elem[p]()/n[l];this[p]=g;return g}else{if(this.applyingMatrix&&this[p]){return this[p]}}var o={height:["top","bottom"],width:["left","right"]};var h=this.$elem[0],j=parseFloat(d.curCSS(h,l,true)),q=this.boxSizingProperty,i=this.boxSizingValue;if(!this.boxSizingProperty){q=this.boxSizingProperty=e()||"box-sizing";i=this.boxSizingValue=this.$elem.css(q)||"content-box"}if(this[p]&&this[l]==j){return 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j=this.attr||this.$elem.attr(a);if(!j||j.indexOf(k)==-1){this.attr=g.trim(j+" "+k+"("+l+")");this.$elem.attr(a,this.attr)}else{var i=[],n;d.lastIndex=0;while(n=d.exec(j)){if(k==n[1]){i.push(k+"("+l+")")}else{i.push(n[0])}}this.attr=i.join(" ");this.$elem.attr(a,this.attr)}},getAttrs:function(){var j=this.attr||this.$elem.attr(a);if(!j){return{}}var i={},l,k;d.lastIndex=0;while((l=d.exec(j))!==null){if(l){k=l[2].split(c);i[l[1]]=k.length==1?k[0]:k}}return i},getAttr:function(j){var i=this.getAttrs();if(typeof i[j]!=="undefined"){return i[j]}if(j==="origin"&&g.support.csstransforms){return this.$elem.css(this.transformOriginProperty).split(e)}else{if(j==="origin"){return["50%","50%"]}}return g.cssDefault[j]||0}});if(typeof(g.cssAngle)=="undefined"){g.cssAngle={}}g.extend(g.cssAngle,{rotate:true,skew:true,skewX:true,skewY:true});if(typeof(g.cssDefault)=="undefined"){g.cssDefault={}}g.extend(g.cssDefault,{scale:[1,1],scaleX:1,scaleY:1,matrix:[1,0,0,1,0,0],origin:["50%","50%"],reflect:[1,0,0,1,0,0],reflectX:[1,0,0,1,0,0],reflectXY:[1,0,0,1,0,0],reflectY:[1,0,0,1,0,0]});if(typeof(g.cssMultipleValues)=="undefined"){g.cssMultipleValues={}}g.extend(g.cssMultipleValues,{matrix:6,origin:{length:2,duplicate:true},reflect:6,reflectX:6,reflectXY:6,reflectY:6,scale:{length:2,duplicate:true},skew:2,translate:2});g.extend(g.cssNumber,{matrix:true,reflect:true,reflectX:true,reflectXY:true,reflectY:true,scale:true,scaleX:true,scaleY:true});g.each(g.transform.funcs,function(j,k){g.cssHooks[k]={set:function(n,o){var l=n.transform||new g.transform(n),i={};i[k]=o;l.exec(i,{preserve:true})},get:function(n,l){var i=n.transform||new g.transform(n);return i.getAttr(k)}}});g.each(["reflect","reflectX","reflectXY","reflectY"],function(j,k){g.cssHooks[k].get=function(n,l){var i=n.transform||new g.transform(n);return i.getAttr("matrix")||g.cssDefault[k]}})})(jQuery,this,this.document);(function(e,g,h,c){var d=/^([+\-]=)?([\d+.\-]+)(.*)$/;var a=e.fn.animate;e.fn.animate=function(p,l,o,n){var k=e.speed(l,o,n),j=e.cssMultipleValues;k.complete=k.old;if(!e.isEmptyObject(p)){if(typeof k.original==="undefined"){k.original={}}e.each(p,function(s,u){if(j[s]||e.cssAngle[s]||(!e.cssNumber[s]&&e.inArray(s,e.transform.funcs)!==-1)){var t=null;if(jQuery.isArray(p[s])){var r=1,q=u.length;if(j[s]){r=(typeof j[s].length==="undefined"?j[s]:j[s].length)}if(q>r||(q<r&&q==2)||(q==2&&r==2&&isNaN(parseFloat(u[q-1])))){t=u[q-1];u.splice(q-1,1)}}k.original[s]=u.toString();p[s]=parseFloat(u)}})}return a.apply(this,[arguments[0],k])};var b="paddingBottom";function i(k,l){if(k[l]!=null&&(!k.style||k.style[l]==null)){}var j=parseFloat(e.css(k,l));return j&&j>-10000?j:0}var f=e.fx.prototype.custom;e.fx.prototype.custom=function(u,v,w){var y=e.cssMultipleValues[this.prop],p=e.cssAngle[this.prop];if(y||(!e.cssNumber[this.prop]&&e.inArray(this.prop,e.transform.funcs)!==-1)){this.values=[];if(!y){y=1}var x=this.options.original[this.prop],t=e(this.elem).css(this.prop),j=e.cssDefault[this.prop]||0;if(!e.isArray(t)){t=[t]}if(!e.isArray(x)){if(e.type(x)==="string"){x=x.split(",")}else{x=[x]}}var l=y.length||y,s=0;while(x.length<l){x.push(y.duplicate?x[0]:j[s]||0);s++}var k,r,q,o=this,n=o.elem.transform;orig=e.style(o.elem,b);e.each(x,function(z,A){if(t[z]){k=t[z]}else{if(j[z]&&!y.duplicate){k=j[z]}else{if(y.duplicate){k=t[0]}else{k=0}}}if(p){k=e.angle.toDegree(k)}else{if(!e.cssNumber[o.prop]){r=d.exec(e.trim(k));if(r[3]&&r[3]!=="px"){if(r[3]==="%"){k=parseFloat(r[2])/100*n["safeOuter"+(z?"Height":"Width")]()}else{e.style(o.elem,b,k);k=i(o.elem,b);e.style(o.elem,b,orig)}}}}k=parseFloat(k);r=d.exec(e.trim(A));if(r){q=parseFloat(r[2]);w=r[3]||"px";if(p){q=e.angle.toDegree(q+w);w="deg"}else{if(!e.cssNumber[o.prop]&&w==="%"){k=(k/n["safeOuter"+(z?"Height":"Width")]())*100}else{if(!e.cssNumber[o.prop]&&w!=="px"){e.style(o.elem,b,(q||1)+w);k=((q||1)/i(o.elem,b))*k;e.style(o.elem,b,orig)}}}if(r[1]){q=((r[1]==="-="?-1:1)*q)+k}}else{q=A;w=""}o.values.push({start:k,end:q,unit:w})})}return f.apply(this,arguments)};e.fx.multipleValueStep={_default:function(j){e.each(j.values,function(k,l){j.values[k].now=l.start+((l.end-l.start)*j.pos)})}};e.each(["matrix","reflect","reflectX","reflectXY","reflectY"],function(j,k){e.fx.multipleValueStep[k]=function(n){var p=n.decomposed,l=e.matrix;m=l.identity();p.now={};e.each(p.start,function(q){p.now[q]=parseFloat(p.start[q])+((parseFloat(p.end[q])-parseFloat(p.start[q]))*n.pos);if(((q==="scaleX"||q==="scaleY")&&p.now[q]===1)||((q!=="scaleX"||q!=="scaleY")&&p.now[q]===0)){return true}m=m.x(l[q](p.now[q]))});var o;e.each(n.values,function(q){switch(q){case 0:o=parseFloat(m.e(1,1).toFixed(6));break;case 1:o=parseFloat(m.e(1,2).toFixed(6));break;case 2:o=parseFloat(m.e(2,1).toFixed(6));break;case 3:o=parseFloat(m.e(2,2).toFixed(6));break;case 4:o=parseFloat(m.e(3,1).toFixed(6));break;case 5:o=parseFloat(m.e(3,2).toFixed(6));break}n.values[q].now=o})}});e.each(e.transform.funcs,function(j,k){e.fx.step[k]=function(o){var n=o.elem.transform||new e.transform(o.elem),l={};if(e.cssMultipleValues[k]||(!e.cssNumber[k]&&e.inArray(k,e.transform.funcs)!==-1)){(e.fx.multipleValueStep[o.prop]||e.fx.multipleValueStep._default)(o);l[o.prop]=[];e.each(o.values,function(p,q){l[o.prop].push(q.now+(e.cssNumber[o.prop]?"":q.unit))})}else{l[o.prop]=o.now+(e.cssNumber[o.prop]?"":o.unit)}n.exec(l,{preserve:true})}});e.each(["matrix","reflect","reflectX","reflectXY","reflectY"],function(j,k){e.fx.step[k]=function(q){var p=q.elem.transform||new e.transform(q.elem),o={};if(!q.initialized){q.initialized=true;if(k!=="matrix"){var n=e.matrix[k]().elements;var r;e.each(q.values,function(s){switch(s){case 0:r=n[0];break;case 1:r=n[2];break;case 2:r=n[1];break;case 3:r=n[3];break;default:r=0}q.values[s].end=r})}q.decomposed={};var l=q.values;q.decomposed.start=e.matrix.matrix(l[0].start,l[1].start,l[2].start,l[3].start,l[4].start,l[5].start).decompose();q.decomposed.end=e.matrix.matrix(l[0].end,l[1].end,l[2].end,l[3].end,l[4].end,l[5].end).decompose()}(e.fx.multipleValueStep[q.prop]||e.fx.multipleValueStep._default)(q);o.matrix=[];e.each(q.values,function(s,t){o.matrix.push(t.now)});p.exec(o,{preserve:true})}})})(jQuery,this,this.document);(function(g,h,j,c){var d=180/Math.PI;var k=200/Math.PI;var f=Math.PI/180;var e=2/1.8;var i=0.9;var a=Math.PI/200;var b=/^([+\-]=)?([\d+.\-]+)(.*)$/;g.extend({angle:{runit:/(deg|g?rad)/,radianToDegree:function(l){return l*d},radianToGrad:function(l){return l*k},degreeToRadian:function(l){return l*f},degreeToGrad:function(l){return l*e},gradToDegree:function(l){return l*i},gradToRadian:function(l){return l*a},toDegree:function(n){var l=b.exec(n);if(l){n=parseFloat(l[2]);switch(l[3]||"deg"){case"grad":n=g.angle.gradToDegree(n);break;case"rad":n=g.angle.radianToDegree(n);break}return n}return 0}}})})(jQuery,this,this.document);(function(e,d,b,f){if(typeof(e.matrix)=="undefined"){e.extend({matrix:{}})}e.extend(e.matrix,{V2:function(g,h){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,2)}else{this.elements=[g,h]}this.length=2},V3:function(g,i,h){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,3)}else{this.elements=[g,i,h]}this.length=3},M2x2:function(h,g,j,i){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,4)}else{this.elements=Array.prototype.slice.call(arguments).slice(0,4)}this.rows=2;this.cols=2},M3x3:function(l,k,j,i,h,g,p,o,n){if(e.isArray(arguments[0])){this.elements=arguments[0].slice(0,9)}else{this.elements=Array.prototype.slice.call(arguments).slice(0,9)}this.rows=3;this.cols=3}});var c={e:function(j,g){var h=this.rows,i=this.cols;if(j>h||g>h||j<1||g<1){return 0}return this.elements[(j-1)*i+g-1]},decompose:function(){var u=this.e(1,1),s=this.e(2,1),p=this.e(1,2),o=this.e(2,2),n=this.e(3,1),l=this.e(3,2);if(Math.abs(u*o-s*p)<0.01){return{rotate:0+"deg",skewX:0+"deg",scaleX:1,scaleY:1,translateX:0+"px",translateY:0+"px"}}var j=n,i=l;var t=Math.sqrt(u*u+s*s);u=u/t;s=s/t;var h=u*p+s*o;p-=u*h;o-=s*h;var q=Math.sqrt(p*p+o*o);p=p/q;o=o/q;h=h/q;if((u*o-s*p)<0){u=-u;s=-s;t=-t}var v=e.angle.radianToDegree;var g=v(Math.atan2(s,u));h=v(Math.atan(h));return{rotate:g+"deg",skewX:h+"deg",scaleX:t,scaleY:q,translateX:j+"px",translateY:i+"px"}}};e.extend(e.matrix.M2x2.prototype,c,{toM3x3:function(){var g=this.elements;return new e.matrix.M3x3(g[0],g[1],0,g[2],g[3],0,0,0,1)},x:function(i){var j=typeof(i.rows)==="undefined";if(!j&&i.rows==3){return this.toM3x3().x(i)}var h=this.elements,g=i.elements;if(j&&g.length==2){return new e.matrix.V2(h[0]*g[0]+h[1]*g[1],h[2]*g[0]+h[3]*g[1])}else{if(g.length==h.length){return new e.matrix.M2x2(h[0]*g[0]+h[1]*g[2],h[0]*g[1]+h[1]*g[3],h[2]*g[0]+h[3]*g[2],h[2]*g[1]+h[3]*g[3])}}return false},inverse:function(){var h=1/this.determinant(),g=this.elements;return new e.matrix.M2x2(h*g[3],h*-g[1],h*-g[2],h*g[0])},determinant:function(){var g=this.elements;return g[0]*g[3]-g[1]*g[2]}});e.extend(e.matrix.M3x3.prototype,c,{x:function(i){var j=typeof(i.rows)==="undefined";if(!j&&i.rows<3){i=i.toM3x3()}var h=this.elements,g=i.elements;if(j&&g.length==3){return new e.matrix.V3(h[0]*g[0]+h[1]*g[1]+h[2]*g[2],h[3]*g[0]+h[4]*g[1]+h[5]*g[2],h[6]*g[0]+h[7]*g[1]+h[8]*g[2])}else{if(g.length==h.length){return new e.matrix.M3x3(h[0]*g[0]+h[1]*g[3]+h[2]*g[6],h[0]*g[1]+h[1]*g[4]+h[2]*g[7],h[0]*g[2]+h[1]*g[5]+h[2]*g[8],h[3]*g[0]+h[4]*g[3]+h[5]*g[6],h[3]*g[1]+h[4]*g[4]+h[5]*g[7],h[3]*g[2]+h[4]*g[5]+h[5]*g[8],h[6]*g[0]+h[7]*g[3]+h[8]*g[6],h[6]*g[1]+h[7]*g[4]+h[8]*g[7],h[6]*g[2]+h[7]*g[5]+h[8]*g[8])}}return false},inverse:function(){var h=1/this.determinant(),g=this.elements;return new e.matrix.M3x3(h*(g[8]*g[4]-g[7]*g[5]),h*(-(g[8]*g[1]-g[7]*g[2])),h*(g[5]*g[1]-g[4]*g[2]),h*(-(g[8]*g[3]-g[6]*g[5])),h*(g[8]*g[0]-g[6]*g[2]),h*(-(g[5]*g[0]-g[3]*g[2])),h*(g[7]*g[3]-g[6]*g[4]),h*(-(g[7]*g[0]-g[6]*g[1])),h*(g[4]*g[0]-g[3]*g[1]))},determinant:function(){var g=this.elements;return g[0]*(g[8]*g[4]-g[7]*g[5])-g[3]*(g[8]*g[1]-g[7]*g[2])+g[6]*(g[5]*g[1]-g[4]*g[2])}});var a={e:function(g){return this.elements[g-1]}};e.extend(e.matrix.V2.prototype,a);e.extend(e.matrix.V3.prototype,a)})(jQuery,this,this.document);(function(c,b,a,d){if(typeof(c.matrix)=="undefined"){c.extend({matrix:{}})}c.extend(c.matrix,{calc:function(e,f,g){this.matrix=e;this.outerHeight=f;this.outerWidth=g}});c.matrix.calc.prototype={coord:function(e,i,h){h=typeof(h)!=="undefined"?h:0;var g=this.matrix,f;switch(g.rows){case 2:f=g.x(new c.matrix.V2(e,i));break;case 3:f=g.x(new c.matrix.V3(e,i,h));break}return f},corners:function(e,h){var f=!(typeof(e)!=="undefined"||typeof(h)!=="undefined"),g;if(!this.c||!f){h=h||this.outerHeight;e=e||this.outerWidth;g={tl:this.coord(0,0),bl:this.coord(0,h),tr:this.coord(e,0),br:this.coord(e,h)}}else{g=this.c}if(f){this.c=g}return g},sides:function(e){var f=e||this.corners();return{top:Math.min(f.tl.e(2),f.tr.e(2),f.br.e(2),f.bl.e(2)),bottom:Math.max(f.tl.e(2),f.tr.e(2),f.br.e(2),f.bl.e(2)),left:Math.min(f.tl.e(1),f.tr.e(1),f.br.e(1),f.bl.e(1)),right:Math.max(f.tl.e(1),f.tr.e(1),f.br.e(1),f.bl.e(1))}},offset:function(e){var f=this.sides(e);return{height:Math.abs(f.bottom-f.top),width:Math.abs(f.right-f.left)}},area:function(e){var h=e||this.corners();var g={x:h.tr.e(1)-h.tl.e(1)+h.br.e(1)-h.bl.e(1),y:h.tr.e(2)-h.tl.e(2)+h.br.e(2)-h.bl.e(2)},f={x:h.bl.e(1)-h.tl.e(1)+h.br.e(1)-h.tr.e(1),y:h.bl.e(2)-h.tl.e(2)+h.br.e(2)-h.tr.e(2)};return 0.25*Math.abs(g.e(1)*f.e(2)-g.e(2)*f.e(1))},nonAffinity:function(){var f=this.sides(),g=f.top-f.bottom,e=f.left-f.right;return parseFloat(parseFloat(Math.abs((Math.pow(g,2)+Math.pow(e,2))/(f.top*f.bottom+f.left*f.right))).toFixed(8))},originOffset:function(h,g){h=h?h:new c.matrix.V2(this.outerWidth*0.5,this.outerHeight*0.5);g=g?g:new c.matrix.V2(0,0);var e=this.coord(h.e(1),h.e(2));var f=this.coord(g.e(1),g.e(2));return{top:(f.e(2)-g.e(2))-(e.e(2)-h.e(2)),left:(f.e(1)-g.e(1))-(e.e(1)-h.e(1))}}}})(jQuery,this,this.document);(function(c,b,a,d){if(typeof(c.matrix)=="undefined"){c.extend({matrix:{}})}c.extend(c.matrix,{identity:function(g){g=g||2;var h=g*g,j=new Array(h),f=g+1;for(var e=0;e<h;e++){j[e]=(e%f)===0?1:0}return new c.matrix["M"+g+"x"+g](j)},matrix:function(){var e=Array.prototype.slice.call(arguments);switch(arguments.length){case 4:return new c.matrix.M2x2(e[0],e[2],e[1],e[3]);case 6:return new c.matrix.M3x3(e[0],e[2],e[4],e[1],e[3],e[5],0,0,1)}},reflect:function(){return new c.matrix.M2x2(-1,0,0,-1)},reflectX:function(){return new c.matrix.M2x2(1,0,0,-1)},reflectXY:function(){return new c.matrix.M2x2(0,1,1,0)},reflectY:function(){return new c.matrix.M2x2(-1,0,0,1)},rotate:function(i){var f=c.angle.degreeToRadian(i),h=Math.cos(f),j=Math.sin(f);var g=h,e=j,l=-j,k=h;return new c.matrix.M2x2(g,l,e,k)},scale:function(f,e){f=f||f===0?f:1;e=e||e===0?e:f;return new c.matrix.M2x2(f,0,0,e)},scaleX:function(e){return c.matrix.scale(e,1)},scaleY:function(e){return c.matrix.scale(1,e)},skew:function(h,f){h=h||0;f=f||0;var i=c.angle.degreeToRadian(h),g=c.angle.degreeToRadian(f),e=Math.tan(i),j=Math.tan(g);return new c.matrix.M2x2(1,e,j,1)},skewX:function(e){return c.matrix.skew(e)},skewY:function(e){return c.matrix.skew(0,e)},translate:function(f,e){f=f||0;e=e||0;return new c.matrix.M3x3(1,0,f,0,1,e,0,0,1)},translateX:function(e){return c.matrix.translate(e)},translateY:function(e){return c.matrix.translate(0,e)}})})(jQuery,this,this.document);</div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.min.js
Team:Hong Kong CUHK/Templates/jquery.min.js
2013-10-28T12:28:51Z
<p>Shengry0716: Created page with "/*! * jQuery JavaScript Library v1.4.3 * http://jquery.com/ * * Copyright 2010, John Resig * Dual licensed under the MIT or GPL Version 2 licenses. * http://jquery.org/lice..."</p>
<hr />
<div>/*!<br />
* jQuery JavaScript Library v1.4.3<br />
* http://jquery.com/<br />
*<br />
* Copyright 2010, John Resig<br />
* Dual licensed under the MIT or GPL Version 2 licenses.<br />
* http://jquery.org/license<br />
*<br />
* Includes Sizzle.js<br />
* http://sizzlejs.com/<br />
* Copyright 2010, The Dojo Foundation<br />
* Released under the MIT, BSD, and GPL Licenses.<br />
*<br />
* Date: Thu Oct 14 23:10:06 2010 -0400<br />
*/<br />
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b],f.body["scroll"+b],f.documentElement["scroll"+b],f.body["offset"+b],f.documentElement["offset"+b]):e===A?parseFloat(c.css(f,d)):this.css(d,typeof e==="string"?e:e+"px")}})})(window);</div>
Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/ianda
Team:Hong Kong CUHK/ianda
2013-10-26T00:34:45Z
<p>Shengry0716: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>iGEM CUHK</title><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/slideshow.css&action=raw&ctype=text/css"><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/indexuse.css&action=raw&ctype=text/css"><br />
<br />
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/search.css&action=raw&ctype=text/css"><br />
<br />
<script src="https://2013.igem.org/wiki/index.php?title=Team:Hong_Kong_CUHK/Templates/jquery-2.0.2.min.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<br />
<script src="https://2013.igem.org/Team:Hong_Kong_CUHK/Templates/jquery.cycle.all.js&action=raw&ctype=text/javascript" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
$('#slider').cycle({<br />
fx: 'scrollHorz', /* Change the fx 'fade' to 'scrollHorz' if you want*/<br />
speed: 'slow',<br />
timeout: 3000,<br />
next: '#next',<br />
prev: '#prev'<br />
});<br />
</script><br />
<script type="text/javascript"><br />
<!--<br />
function MM_showHideLayers() { //v9.0<br />
var i,p,v,obj,args=MM_showHideLayers.arguments;<br />
for (i=0; i<(args.length-2); i+=3) <br />
with (document) if (getElementById && ((obj=getElementById(args[i]))!=null)) { v=args[i+2];<br />
if (obj.style) { obj=obj.style; v=(v=='show')?'visible':(v=='hide')?'hidden':v; }<br />
obj.visibility=v; }<br />
}<br />
//--><br />
</script><br />
<br />
<style type="text/css"><br />
<!--<br />
#p-logo<br />
{visibility:hidden;}<br />
.firstHeading<br />
{visibility:hidden;}<br />
#top-section<br />
{height:2px;border:none;background-color:#660099;}<br />
body{background-color:#660099}<br />
#globalWrapper {<br />
width: 1210px;<br />
height:100%;<br />
}<br />
#search-controls {<br />
display:none;<br />
}<br />
#content<br />
{width: 1000px;border:none;background-color:#660099;padding:0px;margin-top:0px;line-height: 1.5em;color: black;}<br />
#footnote {background-color:#FFFF93;<br />
position:absolute;<br />
left:0;<br />
top:0%;<br />
width:100%;<br />
height:40px;<br />
z-index:4;<br />
text-align:center;<br />
}<br />
#archor {<br />
position:absolute;<br />
right:0%;<br />
top:0%;<br />
width:120px;<br />
height:20px;<br />
z-index:1;<br />
}<br />
body,td,th {<br />
color: #CCC;<br />
}<br />
#containerX2{<br />
position:relative;<br />
width:1210px;<br />
height:100%;<br />
z-index:1;<br />
background-color: #FFF;<br />
}<br />
#partable {<br />
top:75px;<br />
position:absolute;<br />
width:100%;<br />
height:420px;<br />
z-index:11;<br />
}<br />
#heading2{<br />
position:relative;<br />
width:1000px;<br />
height:70px;<br />
z-index:1;<br />
background-color: #FFFFFF;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<div id="banner"><br />
<div id="containerX1" style="margin: 0px auto;"><br />
<div id="logocontainer"><br />
<a name="top" id="top"></a><br />
<div id="swLogo"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/4/4d/Logo_horizontal2.png" width="600" height="170" /></a></div><br />
<div id="iGEMlogo"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png" width="100%" height="100%" /></a></div><br />
<div id="CUHKlogo"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/6/64/Right_logo2.png" width="100%" height="100%" /></a></div><br />
</div> <br />
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<div id="buttons"><br />
<div class="menubar" id="homeUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/e/ee/Bpurple_home.png" width="120" height="76"/></a></div><br />
<div class="menubar" id="teamUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/team"><img src="https://static.igem.org/mediawiki/2013/d/d8/Bpurple_team.png" width="120" height="76" /></a></div><br />
<div class="menubar" id="projectUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/project"><img src="https://static.igem.org/mediawiki/2013/2/2d/Bpurple_project.png" width="120" height="76"/></a></div><br />
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<div class="menubar" id="modelUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/modelling"><img src="https://static.igem.org/mediawiki/2013/3/3f/Bpurple_modelling.png" width="120" height="76"/></a></div><br />
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<div class="menubar" id="ackUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/acknowledgement"><img src="https://static.igem.org/mediawiki/2013/4/4b/Bpurple_ack.png" width="120" height="76"/></a></div><br />
</div><br />
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<div id="buttonsExt"><br />
<div class="buttonbar" id="teamEXT"><a href="member.html" class="linkEXT">Team Members</a> | <a href="ianda.html" class="linkEXT">Instructors and Advisors</a></div><br />
<div class="buttonbar" id="projectEXT"><a href="overview.html" class="linkEXT">Overview</a> | <a href="pah.html" class="linkEXT">PAH Degradtion</a> | <a href="vs.html" class="linkEXT">Voltage Switch</a></div><br />
<div class="buttonbar" id="biobrickEXT"><a href="parts.html" class="linkEXT">Parts</a> | <a href="construction.html" class="linkEXT">Construction Notes</a> | <a href="character.html" class="linkEXT">Characterizations</a></div><br />
<div class="buttonbar" id="docEXT"><a href="protocol.html" class="linkEXT">Protocols</a> | <a href="notebook.html" class="linkEXT">Notebooks</a></div><br />
</div><br />
<br />
</div><br />
<br />
<div id="middle"><br />
<div id="containerX2" style="margin: 0px auto;"><br />
<div id="heading2" style="margin: 0px auto;"><br />
<h2>Instructors and Advisors</h2><br />
</div><br />
<div id="member" style="margin: 0px auto;"><br />
<div id="photo"><img src="https://static.igem.org/mediawiki/2013/4/4e/KT_Chan.JPG" width="271" height="360" hspace="50"></div><br />
<div id="description"><br />
<h1>Prof. CHAN King Ming</h1><br />
<p2><br />
<p>Dr. King Ming Chan is an Associate Professor of School of Life Sciences and Director of Environmental Science Program, Faculty of Science, Chinese University. His research focuses on Aquatic Toxicology and Molecular Endocrinology using fish models. Recent studies are mainly on transgenic fish over-expressing somatolactin hormone for functional study and use of biomarkers of exposures to study the risk of environmental contaminants including pesticides, trace organics, nanoparticles, and metal ions in waters. Dr. Chan also studies the regulation of eukaryotic gene expression and transcription, focusing on understanding how metal-regulatory-element-binding transcription factors (MTFs) control metallothionein genes and how Pit-1s control pituitary polypeptide hormone genes.</p><br />
</p2><br />
</div><br />
</div><br />
<div id="member" style="margin: 0px auto;"><br />
<div id="photo"><img src="https://static.igem.org/mediawiki/2013/c/cc/CHAN_Ting_Fung.JPG" height="100%" hspace="50"/></div><br />
<div id="description"><br />
<h1>Prof. CHAN Ting Fung</h1><br />
<p2><br />
<p>Dr. Ting-Fung Chan is an Assistant Professor in the School of Life Sciences, and the Deputy Director of the Centre for Microbial Genomics and Proteomics at the Chinese University of Hong Kong. He is the Coordinator of the CUHK iGEM team. His researches mainly focus on genomics and bioinformatics of microbial pathogens and complex human phenotypes. He enjoys talking about science, but even more so for toys: Mindstorms NXT, RX-178, 5D Mk-II, LX200-ACF, YAS-875EX, and iGEM. He wishes his students would comprehend the fun and excitement of all aforementioned, but if that is not possible then at the very least, iGEM.</p></p2><br />
</div><br />
</div><br />
<br />
<div id="member" style="margin: 0px auto;"><br />
<div id="photo"><img src="https://static.igem.org/mediawiki/2013/1/1b/Kevin_Yip.JPG" height="100%" hspace="50" vspace="0" /></div><br />
<div id="description"><br />
<h1>Prof. Kevin Yuk-Lap YIP</h1><br />
<p2><br />
<p>Dr. Kevin Yio is an assistant professor in the Deprtment of Computer Science and Engineering of the Chinese University of Hong Kong. He has been researching in the field of bioinformatics for 9 years, since he was a graduate student first in Hong Kong and later at Yale University. He is particularly interested in modeling and studying the interactions of biological objects in large-scale networks, such as protein-protein interaction, gene regulatory and metabolic networks. His major role in the CUHK iGEM team is to introduce the competition to engineering students, recruit interested and committed individuals, and help them to enrich the engineering aspects of the project.</p><br />
</p2><br />
</div><br />
</div><br />
<br />
<br />
<br />
<div id="member" style="margin: 0px auto;"><br />
<div id="photo"><img src="https://static.igem.org/mediawiki/2013/c/cf/Douglas.JPG" height="100%" hspace="50"></div><br />
<div id="description"><br />
<h1>Prof. Pun To, Douglas YUNG</h1><br />
<p2><p>Dr. Douglas Yung is an Assistant Professor in the CUHK Biomedical Engineering Program. He has been intrigued by biosensing and electronic interfacing of living cells since graduate school. On the biosensing side, his research group is developing technologies for rapid viability assessment of superbugs using a combination of electrochemical, optical techniques and MEMS devices. His team is also developing methods to interface living microbes with electronics at a micro- and nano-scale. As for this year&rsquo;s iGEM, he primarily provides support on the device and engineering side.</p><br />
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<h1>Stephen</h1><br />
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<p>Full name: Leung King Pong, Stephen</p><br />
<p><br />
Programme of Study: Life Science PG1</p><br />
<p>Hi! I'm Stephen, currently a graduate student focusing on plant cell biology research. Apart from having a taste of synthetic biology, which constantly surprises me with fascinating ideas, I'm honoured to guide a team of enthusiastic students with great perseverance. I hope the team can enjoy themselves and I'm looking forward to seeing other new ideas in the coming competition!</p><br />
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<h1>Mary</h1><br />
<p2><br />
<p>Full name: ZHU Lin</p><br />
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<p><br />
<p2>Hi, I am a 2nd year PhD student studying Bioinformatics at CUHK. My research interests focus on cancer genetics and genomics, next generation sequencing analysis, and computational biology. I am very glad to join this team. I appreciate the opportunity to participate in the iGEM competition, which is an icon of innovation. In my spare time, I enjoy reading, travelling, playing badminton, dancing and hiking. <br />
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<h1>Jacky</h1><br />
<p2><br />
<p>Full name: Jacky, Fong Chuen, Loo</p><br />
<p>Programme of study: Life Science PG2</p><br />
<p>Jacky is a third-year PhD student in School of Life Science at CUHK. His research focus is aptamer technology and its integration on bio-sensors development for downstream applications. He also studies the mechanism of multi-drug resistant cancer, focusing on the organelle-specific targeted drug action.</p><br />
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Address: Rm. 184, Science Centre, CUHK <br/><br />
Email: kingchan@cuhk.edu.hk Tel: (852)-39434420 Fax: (852)-26037246<br />
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Shengry0716
http://2013.igem.org/File:Logo_horizontal2.png
File:Logo horizontal2.png
2013-10-22T08:04:15Z
<p>Shengry0716: uploaded a new version of &quot;File:Logo horizontal2.png&quot;</p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK
Team:Hong Kong CUHK
2013-10-22T07:30:53Z
<p>Shengry0716: </p>
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<p2>Polycyclic aromatic hydrocarbons (PAHs) are harmful to both environment and human health. We proposed the PAHs degradation system, which contains codon-optimized laccase from Bacillus sp. HR03 and catechol 1,2-dioxygenase from Pseudomonas putida KT2440 for Escherichia coli. The...</p2><br />
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<p2>Interest and mindset formation should start at young age for permanence and continuity. High school and university students are great audiences to be approached to break the blurred paradigm of synthetic biology, which term is rarely known by the general public...</p2><br />
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<h1>Voltage Switch</h1><br />
<p2>The voltage-dependent ion channels family are a group of ion channel proteins found to have permeability to ions dependent on the voltage in the external environment. This family of ion channels are widely found in neuronal cells...</p2><br />
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<p2>Our project initiated from dealing with toxic chemicals found in second-hand smoke (or environmental tobacco smoke, ETS) and cooking fume. After carefully examining the composition of pollutes, we noticed that there was one group of highly toxic...</p2><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK
Team:Hong Kong CUHK
2013-10-22T07:28:36Z
<p>Shengry0716: </p>
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<p2>Interest and mindset formation should start at young age for permanence and continuity. High school and university students are great audiences to be approached to break the blurred paradigm of synthetic biology, which term is rarely known by the general public...</p2><br />
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<p2>The voltage-dependent ion channels family are a group of ion channel proteins found to have permeability to ions dependent on the voltage in the external environment. This family of ion channels are widely found in neuronal cells...</p2><br />
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<h1>PAH Degradation</h1><br />
<p2>Our project initiated from dealing with toxic chemicals found in second-hand smoke (or environmental tobacco smoke, ETS) and cooking fume. After carefully examining the composition of pollutes, we noticed that there was one group of highly toxic...</p2><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK
Team:Hong Kong CUHK
2013-10-22T07:26:48Z
<p>Shengry0716: </p>
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<p2>Polycyclic aromatic hydrocarbons (PAHs) are harmful to both environment and human health. We proposed the PAHs degradation system, which contains codon-optimized laccase from Bacillus sp. HR03 and catechol 1,2-dioxygenase from Pseudomonas putida KT2440 for Escherichia coli. The...</p2><br />
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<p2>Interest and mindset formation should start at young age for permanence and continuity. High school and university students are great audiences to be approached to break the blurred paradigm of synthetic biology, which term is rarely known by the general public...</p2><br />
</a><br />
</div><br />
<div id="Voltage"><br />
<a href="https://2013.igem.org/Team:Hong_Kong_CUHK/backgroundVS"><br />
<h1>Voltage Switch</h1><br />
<p2>The voltage-dependent ion channels family are a group of ion channel proteins found to have permeability to ions dependent on the voltage in the external environment. This family of ion channels are widely found in neuronal cells...</p2><br />
<p>&nbsp;</p><br />
</a><br />
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<h1>PAH Degradation</h1><br />
<p2>Our project initiated from dealing with toxic chemicals found in second-hand smoke (or environmental tobacco smoke, ETS) and cooking fume. After carefully examining the composition of pollutes, we noticed that there was one group of highly toxic...</p2><br />
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Shengry0716
http://2013.igem.org/Hong_Kong_CUHK/hp
Hong Kong CUHK/hp
2013-10-22T07:25:44Z
<p>Shengry0716: </p>
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/august
Team:Hong Kong CUHK/august
2013-10-22T07:24:12Z
<p>Shengry0716: </p>
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<h2>August</h2><br />
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<br />
<p2><p>Aug 1 </p><br />
<p>Transformation: M2, M3, QsrR, 23100 </p><br />
<p>Pick clones: Dioxgenase, Laccase from yesterday plates. </p><br />
<p>Miniprep of Laccase and dioxgenase and gel electrophoresis (no result). </p><br />
<p>PCR: PolyProline linker; run gel, seemed to be successful, but ladder didn’t look right since bands were missing. </p><br />
<p>&nbsp;</p><br />
<p>Aug 2 </p><br />
<p>1. Miniprep of Laccase and Dioxgenase </p><br />
<p>2. PCR of GGGGS linkers and Polyproline linkers </p><br />
<p>&nbsp;</p><br />
<p>Aug 3 </p><br />
<p>1. Restriction digestion of Laccase and Dioxygenase </p><br />
<p>2. Run gel: Dioxgenase, Laccase, PCR products </p><br />
<p>&nbsp;</p><br />
<p>Aug 4 </p><br />
<p>1. Picked colonies from M2, M3, 23100 and QsrR. </p><br />
<p>2. Miniprep. </p><br />
<p>3. Run gel, no result. </p><br />
<p>&nbsp;</p><br />
<p>Aug 5 </p><br />
<p>1. PCR of SsDsbA, TM Helix, Voltage sensor peptide. </p><br />
<p>2. Run gel </p><br />
<p>3. Gel clean </p><br />
<p>4. Ligation </p><br />
<p>5. Transformation </p><br />
<p>&nbsp;</p><br />
<p>Aug 6 </p><br />
<p>Pick clones from SsDsbA, TM Helix, Voltage sensor peptide </p><br />
<p>Transformation of M2, M3, QsrR, 23100 </p><br />
<p>&nbsp;</p><br />
<p>Aug 7 </p><br />
<p>T4 ligation of Dioxygenase, laccase, QsrR, T7+RBS+Dioxygenase, laccase, QsrR. </p><br />
<p>DNA clean </p><br />
<p>Transformation </p><br />
<p>Pick M2, M3, QsrR, 23100 </p><br />
<p>&nbsp;</p><br />
<p>Aug 8 </p><br />
<p>Miniprep of M2, M3, QsrR, 23100 -&gt; run gel -&gt; fail. </p><br />
<p>Colony-PCR: Dioxygenase, laccase, QsrR -&gt; run gel -&gt; fail. </p><br />
<p>Transformation of Dioxygenase, laccase, QsrR, T7+RBS+Dioxygenase, laccase, QsrR. </p><br />
<p>Lab Workshop and talk. </p><br />
<p>&nbsp;</p><br />
<p>Aug 9 </p><br />
<p>Restriction digestion of PSB1C3, PSB1C3-T7-RBS, Laccase, Dioxgenase, QsrR </p><br />
<p>Purification with gel purification kit </p><br />
<p>T4 Ligation </p><br />
<p>Transformation </p><br />
<p>&nbsp;</p><br />
<p>Aug 10 </p><br />
<p>Colony PCR on T7-RBS- {dioxgenase, QsrR, PP, GG, VS, TM}. </p><br />
<p>Run gel </p><br />
<p>&nbsp;</p><br />
<p>Aug 11 </p><br />
<p>Colony PCR on T7-RBS-{PP, GG, Laccase}, TM </p><br />
<p>Run gel </p><br />
<p>Pick colonies from all suspected result colonies </p><br />
<p>&nbsp;</p><br />
<p>Aug 12 </p><br />
<p>Miniprep on T7-RBS-{PP,GG, QsrR, VS, TM} </p><br />
<p>Result: QsrR with T7-RBS. </p><br />
<p>è PSB1C3-T7RBS-QsrR attained. </p><br />
<p>New round of Biobrick: </p><br />
<p>Digestion, Ligation, Transformation of PSB1C3+T7RBC+Laccase/Dioxgenase/QsrR </p><br />
<p>PSB1C3+ PP/GG/VS/TM/Lac/Dioxy/QsrR </p><br />
<p>&nbsp;</p><br />
<p>Aug 13 </p><br />
<p>Colony PCR on PSB1C3-T70RBS- Lac/Dioxy/QsrR and PSB1C3- VS/PP/GGGGS/TM/Lac/Dioxy/QsrR (no result) </p><br />
<p>&nbsp;</p><br />
<p>Aug 15 </p><br />
<p>Colony PCR on PSB1C3-T7-RBS-Lac/Dioxy; PSB1C3- VS/TM/Lac/Dioxy/QsrR </p><br />
<p>è PSB1C3-TM/QsrR got results. </p><br />
<p>&nbsp;</p><br />
<p>Aug 16 </p><br />
<p>Miniprep: TM+QsrR </p><br />
<p>Run gel+ digestion. </p><br />
<p>PCR on Dioxy, Lac, SsDsBa, PDZ domain, SSDsBa PDZ Ligand, SSDsBa PDZ domain+ FL3-Lgt-FL3, SSDsBa PDZ Ligand+FL3-Lgt-FL3 </p><br />
<p>&nbsp;</p><br />
<p>Aug 18 </p><br />
<p>PCR for Dioxygenase, Voltage sensor, TM Helix, PP, GGGGS, PDZ domain+Lgt, +Laccase and PDZ Ligand+ Lgt </p><br />
<p>Purification, cut, purification, ligation, transformation to PSB1C3 or PSB1C3-T7-RBS </p><br />
<p>No result </p><br />
<p>&nbsp;</p><br />
<p>Aug 20 </p><br />
<p>PCR for Dioxygenase, PDZ Domain (With and without Lgt), PDZ Ligand </p><br />
<p>Purification </p><br />
<p>Cut Dioxygenase, Domain +/- Lgt, Ligand +/- Lgt, Laccase, Voltage Sensor, TM Helix, PP, GGGGS </p><br />
<p>Gel Purification for Dioxgenase. </p><br />
<p>Miniprep M2+M3 for purification. </p><br />
<p>&nbsp;</p><br />
<p>Aug 21 </p><br />
<p>PCR, cut, ligation, transformation of Di-C, Di-7, La-C, La-7, LL-7, LL-C, V-C, T-C, P-C, G-C: no clone. </p><br />
<p>&nbsp;</p><br />
<p>Aug 22 </p><br />
<p>PCR DL, D, L with KAPA HiFi Pol </p><br />
<p>Cut, ligation, transformation of Di-C, Di-7, La-C, La-7, LL-7, LL-C, V-C, T-C, P-C</p><br />
</p2><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/july
Team:Hong Kong CUHK/july
2013-10-22T07:23:25Z
<p>Shengry0716: </p>
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<h2>July</h2><br />
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<p2><p>July 2 </p><br />
<p>1. Restriction digestion of T7RBS plasmid with E and P in buffer 2. Prepared by BL21 and DH5 </p><br />
<p>2. Run gel in the following order: </p><br />
<p>Ladder (100-6000bp), new T7-RBS complete, new T7-RBS cut, old T7-RBS complete, old T7-RBS cut, psB1K3+RFP complete. </p><br />
<p>Seems like it fails as well. We may need to redo transformation protocol for T7-RBS. </p><br />
<p>&gt; Gel photo 2 </p><br />
<p>&nbsp;</p><br />
<p>July 3 </p><br />
<p>1. Transformation of T7-RBS into DH5 </p><br />
<p>2. Transformation of pSB1C3-RFP into DH5 and BL21 to test for competency. </p><br />
<p>&nbsp;</p><br />
<p>July 4 </p><br />
<p>1. pSB1C3-RFP in DH5 gave false clone, and there were no colony in BL21 plate, so the cells were bad. We need to redo competent cells again. </p><br />
<p>2. T7-RBS in DH5 shows colonies with a lot of satellites. Large colonies were picked out of each plate and shaked with C antibiotic. </p><br />
<p>3. DGS students came to lab for lab visit. They saw demonstration of picking colonies. </p><br />
<p>&nbsp;</p><br />
<p>July 5 </p><br />
<p>1. Finished preparation of T7-RBS. </p><br />
<p>2. CCMB80 prepared. </p><br />
<p>3. Ran gel with restriction digestion with EcoRI and PstI confirmed presence of T7-RBS. </p><br />
<p>&gt; Gel photo 3 </p><br />
<p>&nbsp;</p><br />
<p>July 8 </p><br />
<p>Streak plates with DH5 and BL21. </p><br />
<p>&nbsp;</p><br />
<p>July 9 </p><br />
<p>Pick colonies from DH5 and BL21 plates. 2 flasks of LB(each 275ml) is being autoclaved for tomorrow use. </p><br />
<p>&nbsp;</p><br />
<p>July 10 </p><br />
<p>Competent cells (BL21 + DH5 ) made. </p><br />
<p>Transformation with pSB1C3+RFP. </p><br />
<p>July 11 </p><br />
<p>Picked colonies and shaked overnight from pSB1c3_RFP in old DH5 . There was no result from the old BL21 and the new completent cells will try again on monday. </p><br />
<p>&nbsp;</p><br />
<p>July 12 </p><br />
<p>Miniprep and ran gel with pSB1C3_RFP. Correct bandings were observed with an unknown band at 6000 or higher. </p><br />
<p>&gt;Gel photo 4 </p><br />
<p>&nbsp;</p><br />
<p>July 15 </p><br />
<p>Transformation on 23100 nto DH5 . </p><br />
<p>&nbsp;</p><br />
<p>July 16 </p><br />
<p>PCR with primers of Lgt. </p><br />
<p>Ran gel, a band of 100bp obtained which is not the desired band. </p><br />
<p>Slight band smaller than 100bp is from primers, bright band of around 100bp is from self annealing of the reverse single strand of around 1000bp probably. </p><br />
<p>&gt;Gel photo 5 </p><br />
<p>&nbsp;</p><br />
<p>July 17 </p><br />
<p>Human practice: Lab workshop and talk. </p><br />
<p>&gt;Gel photo 6 </p><br />
<p>&gt;Gel photo 7 </p><br />
<p>&nbsp;</p><br />
<p>July 19 </p><br />
<p>Restriction digestion and run gel, no bands showed up. It was later found that C18 from plate 1 is used, and the J23100 is in plate 5. </p><br />
<p>&gt;Gel photo 8 </p><br />
<p>&nbsp;</p><br />
<p>July 22 </p><br />
<p>Transformation with J23100. </p><br />
<p>Prepare for lab workshop. </p><br />
<p>&nbsp;</p><br />
<p>July 23 </p><br />
<p>The plate was full of bacteria, probably because antibiotic is added too early when the agar is still too hot. Rehearsal for lab workshop. </p><br />
<p>&nbsp;</p><br />
<p>July 24 </p><br />
<p>Prepare for workshop. </p><br />
<p>Picking colonies, only 5 colonies are found in old plate. </p><br />
<p>&nbsp;</p><br />
<p>July 25 </p><br />
<p>Human practice: workshop and talk. </p><br />
<p>Miniprep. </p><br />
<p>&nbsp;</p><br />
<p>July 26 </p><br />
<p>Run gel, restriction digestion. Get no result. </p><br />
<p>Move all stuff in BME lab back. Move PCR machine to lab. </p><br />
<p>&nbsp;</p><br />
<p>July 29 </p><br />
<p>Transformation: </p><br />
<p>A: J23100 from plate 5, J23100 provided by Isaac, M2 from SJTU, M3 from SJTU; </p><br />
<p>C: RFP (test competency) </p><br />
<p>Streak plate for making competent cells. </p><br />
<p>&nbsp;</p><br />
<p>July 30 </p><br />
<p>Prepare buffer for making competent cells. </p><br />
<p>Pick colonies. </p><br />
<p>Transformation: </p><br />
<p>A: M2, M3, 23100, QsrR </p><br />
<p>C: RFP (test) </p><br />
<p>K: Laccase, Dioxygenase </p><br />
<p>&nbsp;</p><br />
<p>July 31 </p><br />
<p>Transformation: M2,M3, 23100, QsrR, RFPx2(Test) (failed) </p><br />
<p>Pick colonies: Laccase, Dioxygenase </p><br />
<p>Miniprep of M2x2+23100+QsrR and gel electrophoresis (no result) </p><br />
<p>Make competent cells. </p><br />
</p2><br />
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Shengry0716
http://2013.igem.org/Team:Hong_Kong_CUHK/protocol
Team:Hong Kong CUHK/protocol
2013-10-22T07:21:16Z
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<p3><br />
<p><strong>1. Cloning</strong><br /><br />
<strong><a href="#a1.1">1.1 Gene Amplification with PCR</a></strong><br /><br />
<strong><a href="#a1.2">1.2 PCR Purification ( Using TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0)</a></strong><br /><br />
<strong><a href="#a1.3">1.3 Overlapping PCR</a></strong><br /><br />
<strong><a href="#a1.4">1.4 Agarose Gel Electrophoresis</a></strong><br /><br />
<strong><a href="#a1.5">1.5 Preparation of Competent Cells</a></strong><br /><br />
<strong><a href="#a1.6">1.6 Bacterial Transformation</a></strong><br /><br />
<strong><a href="#a1.7">1.7 Colony PCR</a></strong><br /><br />
<strong><a href="#a1.8">1.8 Inoculation</a></strong><br /><br />
<strong><a href="#a1.9">1.9 Plasmid DNA Extraction (Using TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0)</a></strong><br /><br />
<strong><a href="#a1.10">1.10 Double Digestion</a></strong><br /><br />
<strong><a href="#a1.11">1.11 Gel Extraction</a></strong><br /><br />
<strong><a href="#a1.12">1.12 Ligation</a></strong></p><br />
<p><strong>2. Functional test</strong><br /><br />
<strong><a href="#a2.1">2.1 Cell Viability Test with Voltage Applied</a></strong><br />
<br /><br />
<strong><a href="#a2.2">2.2 Cell Viability Test with BaP</a></strong></p><br />
</p3><br />
<div><br />
<hr size="1" width="100%" noshade="noshade" align="left" /><br />
</div><br />
<p><a name="a1.1" id="a1.1"></a><h4>1.1 Gene Amplification with PCR </h4><br />
<p2><br />
1. Put the reaction tubes on ice.<br /><br />
2. Add the following components into each reaction tube:</p><br />
<ul><br />
<li>5x Phusion HF Buffer* (1X final concentration is recommended)</li><br />
<li>10 mM dNTPs (200 μM final concentration is recommended)</li><br />
<li>Primer solution (0.5 μM final concentration of each is recommended)</li><br />
<li>DNA Template</li><br />
<li>Phusion DNA Polymerase (0.02 U/μl final concentration is recommended)</li><br />
</ul><br />
<p>3. Mix well and run the following program:</p><br />
<p>2-step PCR Cycling Program<br /><br />
Initial denaturation:<br /><br />
98°C&nbsp;&nbsp; 30 seconds<br /><br />
25–35 cycles<br /><br />
98°C&nbsp; &nbsp;5–10 seconds<br /><br />
72°C&nbsp;&nbsp; 15–30 seconds/kb<br /><br />
Final extension:<br /><br />
72°C&nbsp;&nbsp; 5–10 minutes<br /><br />
Hold:<br /><br />
4°C<br /><br />
</p2><br />
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<p><a name="a1.2" id="a1.2"></a><h4><strong>1.2 PCR Purification</strong><strong> ( Using TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0)</strong></h4><br />
<p2><br />
1. Set a Spin Column into Collection Tube.<br /><br />
2. Add 3X sample volume’s Buffer GM to the eppendorf containing PCR product. Mix it well and transfer the mixture into the Spin Column and centrifuge at 12,000 rpm for 1 min. For improvement the recovery rate of DNA, transfer the flow-through to Spin Column again and centrifuge again. Discard the flow-through. <br /><br />
3. Add 700 μl of Buffer WB into the Spin Column. Centrifuge at 12,000 rpm for 30 seconds.<br /><br />
Discard the flow-through.<br /><br />
Note: Make sure that the amount of 100% ethanol specified on the bottle label has<br /><br />
been added to the Buffer WB.<br /><br />
4. Repeat Step 10.<br /><br />
5. Place the Spin Column back into Collection Tube. Centrifuge at 12,000 rpm for 1 minute.<br /><br />
6. Place the Spin Column into a new 1.5 ml tube. Add 20 μl of 60℃ sterile distilled water to the center of the membrane. Let it stand for 1 minute at room temperature and then centrifuge.<br /><br />
7. Take back the flow-through to the center of the membrane. Let it stand for 1 minute at room temperature and then centrifuge again.<br /><br />
</p2><br />
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<a name="a1.3" id="a1.3"></a><h4><strong>1.3 Overlapping PCR</strong></h4><br /><br />
<p2><br />
1. Put the reaction tubes on ice.<br /><br />
2. Add the following components into each reaction tube:</p><br />
<ul><br />
<li>5x Phusion HF Buffer* (1X final concentration is recommended)</li><br />
<li>10 mM dNTPs (200 μM final concentration is recommended)</li><br />
<li>Primer solution (0.5 μM final concentration of each is recommended)</li><br />
<li>DNA Template</li><br />
<li>Phusion DNA Polymerase (0.02 U/μl final concentration is recommended)</li><br />
</ul><br />
<p>3. Mix well and run the following program:<br /><br />
2-step PCR Cycling Program<br /><br />
Initial denaturation:<br /><br />
98°C&nbsp;&nbsp; 30 seconds<br /><br />
25–35 cycles:<br /><br />
98°C&nbsp; &nbsp;10 seconds (denaturation)<br /><br />
60°C&nbsp; 10 seconds (annealing)<br /><br />
72°C&nbsp;&nbsp; 15–30 seconds/kb (extension)<br /><br />
Hold:<br /><br />
4°C<br /><br />
</p2> <br />
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<p><a name="a1.4" id="a1.4"></a><h4><strong>1.4 Agarose Gel Electrophoresis</strong></h4><br /><br />
<p2><br />
<strong>A.&nbsp;</strong>Cast gel<br /><br />
1. Dissolve 0.55 g agarose into 55 ml 0.5X TBE buffer.<br /><br />
2. Microwave (high power, 800W) for 1 min.<br /><br />
3. Cool it down using running water for 1 min.<br /><br />
4. Add 1 μl GelRed<br /><br />
5. Pour the solution to tightened tank with gates and gel comb and allow it to solidify.<br /><br />
6. Transfer the gel to gel tank once it becomes solid.<br /><br />
<strong>B.&nbsp;</strong>Run gel<br /><br />
1. Orient the gel with wells facing the black negative electrode. Check if the gel is covered by TBE buffer in the tank. If not, add TBE buffer to cover it to about 1mm.<br /><br />
2. Mix loading dye and the insert/plasmid before adding to the wells. For example, if the DNA we have got is 45μl, and the loading dye we have got is 10X, then add 5μl of loading dye to the samples. Mixture should be in blue.<br /><br />
3. To run the gel, add all samples to the wells of gel. Then add 1kb DNA ladder to a separate well. 1μl should be enough for detection under UV.<br /><br />
4. Connect the electrodes to the power supply with correct colour. Set the power supply to 120V. Check if there are bubbles on the negative electrodes.<br /><br />
5. Allow it to run for about 15-30min. To avoid running the band off the gel, the yellow band (position of the smallest fragments) should stay on the gel.</p><br />
</p2><br />
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<p><a name="a1.5" id="a1.5"></a><h4><strong>1.5 Preparation of Competent Cells</strong></h4><br /><br />
<p2><br />
<strong>A.&nbsp;</strong>Preparation of detergent-free glassware and media<br /><br />
1. Autoclave glassware filled with 3/4 dd-H2O to remove most residual detergent.<br /><br />
2. Autoclave media and buffers in detergent-free glassware<br /><br />
<strong>B.</strong>&nbsp;Preparation of the competent cells<br /><br />
Reagents:</p><br />
<ul><br />
<li>Glycerol stock</li><br />
<li>LB plate</li><br />
<li>MgCl2-CaCl2 solution</li><br />
<ul><br />
<li>MgCl2‧6H2O&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 3.25g</li><br />
<li>CaCl2‧2H2O&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 0.6g</li><br />
<li>Add H2O to 200 ml</li><br />
</ul><br />
<li>100mM CaCl2</li><br />
<ul><br />
<li>CaCl2‧2H2O&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2.95g</li><br />
<li>Add H2O to 200 ml</li><br />
</ul><br />
<li>80% glycerol</li><br />
<li>Liquid nitrogen</li><br />
</ul><br />
<p>Procedure:<br /><br />
<strong>Day 1</strong><br /><br />
1. Flame the metal inoculating loop until it is red got and then cools it down.<br /><br />
2. Scrape off a portion from the top of the frozen glycerol stock [DO NOT THAW].<br /><br />
3. Streak it onto the LB plate.<br /><br />
4. Put the stock back to -80 oC immediately.<br /><br />
5. Leave the plates for 5 min and place them upside down in the 37oC incubator for 16-20 h.<br /><br />
<strong>Day 2</strong> <br /><br />
6. Pick a single colony into 5 ml of LB medium.<br /><br />
7. Inoculate the culture overnight at 37oC with shaking at 250 rpm.<br /><br />
<strong>Day 3</strong><br /><br />
8. Inoculate 100 ml LB medium with 1 ml of saturated overnight culture.<br /><br />
9. Shake at 37oC until OD600 = 0.4 (usually 2-3 h).<br /><br />
10. Place in an ice bath for 10 min.<br /><br />
[After this point, the cells must be placed on ice!]<br /><br />
11. Pre-cool solution, centrifuge, pipette tips, falcon, and eppendorf.<br /><br />
12. Transfer the culture into two pre-chilled 50ml falcon.<br /><br />
13. Centrifuge at 2700 x g for 10 min at 4oC<br /><br />
14. Remove the medium, and resuspend the cell pellet with 1.6 ml ice-cold 100 mM CaCl2 by swirling on ice gently.<br /><br />
15. Incubate on ice for 30 min.<br /><br />
16. Centrifuge at 2700 x g for 10 minutes at 4oC.<br /><br />
17. Remove the medium, and resuspend the cell pellet with 1.6 ml ice-cold 100 mM CaCl2 by swirling on ice gently.<br /><br />
18. Incubate on ice for 20 min.<br /><br />
19. Pool all cells into one tube and add 0.5 ml ice-cold 80% glycerol and swirl to mix.<br /><br />
20. Freeze 100 μl aliquots in liquid nitrogen.<br /><br />
21. Store in -80oC.</p><br />
</p2><br />
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<p><a name="a1.6" id="a1.6"></a><h4><strong>1.6 Bacterial Transformation</strong></h4><br /><br />
<p2><br />
1. Thaw competent cell on ice.<br /><br />
2. Add 50 - 100 ng DNA to competent cell culture.<br /><br />
3. Put in ice for 10min<br /><br />
4. Heat shock at 42oC for 1.5-2 min<br /><br />
5. Put in ice for 2 min.<br /><br />
6. Add 1 ml LB medium.<br /><br />
7. Incubate at 37oC for 30-90 min with shaking (~ 250 rpm).<br /><br />
8. Spread plate (with suitable antibiotics)<br /><br />
9. Spin down the remaining cells and discard large amount supernatant (1 ml).<br /><br />
10. Resuspend the cell pellet and spread plate.<br /><br />
11. Incubate at 37oC overnight (preferably ~16 – 24h).</p><br />
<p>&nbsp;</p><br />
</p2><br />
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<p><a name="a1.7" id="a1.7"></a><h4><strong>1.7Colony PCR</strong></h4><br /><br />
<p2><br />
1. Put the reaction tubes on ice.<br /><br />
2. Add the following components into each PCR tube:<br /><br />
Acutoclaved water 2.3μl<br /><br />
rTaq 2.5μl<br /><br />
Primers(BBa-G1004F+BBa-G1005R) 0.1μl +0.1μl<br /><br />
3. Pick Colonies into each PCR tube. Be careful to label it well.<br /><br />
3. Mix well and run the following program:<br /><br />
<br /><br />
Heatlid <br /><br />
104°C<br /><br />
Initial denaturation:<br /><br />
94°C 5min<br /><br />
20–25 cycles<br /><br />
94°C 30 seconds<br /><br />
63°C 30 seconds<br /><br />
Final extension:<br /><br />
72°C 1min10sec<br /><br />
Hold:<br /><br />
4°C</p><br />
</p2><br />
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<p><a name="a1.8" id="a1.8"></a><h4><strong>1.8 Inoculation</strong></h4><br />
<p><br /><br />
<p2><br />
1. Pick colonies and culture in 3-5 ml LB broth with antibiotics. (1μl/1ml) <br /><br />
2. Incubate at 37oC for 12 - 16 h with shaking at 250 rpm.</p><br />
</p2><br />
</p><br />
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</p><br />
</p><br />
<p><a name="a1.9" id="a1.9"></a><h4><strong>1.9 Plasmid DNA Preparation (using TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0)</strong></h4></p><br />
<p2><br />
<p>1. Use 1 - 4 ml oft heE. coli culture. Centrifuge at 12,000 rpm for 2 minutes to harvest<br /><br />
the cell. Discard the supernatant.<br /><br />
2. Add 250μl Solution l (containing RNaseA). Resuspend the bacterial cell pellet completely by vortexing or pipetting up and down.<br /><br />
Note: Be sure thatthe bacteria are completely resuspended by vortexing and no<br /><br />
cell clumps remain before addition of Solution ll.<br /><br />
3. Add 250μl Solution ll, and mix gently by inverting the tube 5 - 6 times to completely lysis the cell untilthe solution becomes viscous and slightly clear.<br /><br />
Note: Do not allowthe lysis reaction to proceed more than 5 minutes.<br /><br />
4. Add 350μl of 4℃precooling Solution lll, and mix immediately and thoroughly by<br /><br />
inverting the tube 5 - 6 times until a compactwhite pellet has been formed. Incubate atroom temperature for 2 minutes.<br /><br />
5. Centrifuge at 12,000 rpm atroom temperature for 10 minutes.<br /><br />
Note: Centrifuging at 4℃is notrecommended for precipitation.<br /><br />
6. Place a Spin Column in a Collection Tube.<br /><br />
7. Apply the supernatantfrom Step 6 onto the Spin Column by decant or pipetting.<br /><br />
Centrifuge at 12,000 rpm for 1 minute. Discard the flow-through.<br /><br />
8. Pipette 500μl of Buffer WAonto the Spin Column. Centrifuge at 12,000 rpm for 30<br /><br />
seconds. Discard the flow-through.<br /><br />
9. Pipette 700μl of Buffer WB onto the Spin Column. Centrifuge at 12,000 rpm for 30<br /><br />
seconds. Discard the flow-through.<br /><br />
Note: Make sure thatthe amount of 100% ethanol indicated on the bottle label has<br /><br />
been added to Buffer WB.<br /><br />
10. Repeat Step 10.<br /><br />
11. Place the Spin Column back into the Collection Tube. Centrifuge at 12,000 rpm for<br /><br />
an additional 1 minute to remove residual wash Buffer WB.<br /><br />
Note: Residual ethanol from Buffer WB may inhibit subsequent enzymatic reaction.<br /><br />
12. Place the Spin Column in a new clean 1.5 ml tube.<br /><br />
Add 25μl Elution Buffer or sterile 60℃distilled water to the center of the Spin Column<br /><br />
membrane. Incubate for 1 minute atroom temperature. Centrifuge at 12,000 rpm for 1 minute to elute DNA<br /><br />
13. Repeat step 12.</p><br />
</p2><br />
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<p><a name="a1.10" id="a1.10"></a><h4><strong>1.10 Restriction Digestion</strong></h4><br /><br />
<p2><br />
1. Mix the components as follows to prepare a 50 μl reaction mixture:<br /><br />
15μl Insert/Vector# + ddH2O*<br /><br />
2 μl 10X NEB Buffer2<br /><br />
2 μl 10X BSA<br /><br />
0.5 μl Enzyme 1<br /><br />
0.5 μl Enzyme 2<br /><br />
# At least 200ng DNA should be added<br /><br />
* Water is added first and the template the last<br /><br />
2. Incubate the reaction mixture at 37oC for 1 h.<br /><br />
Buffer Chart<br /><br />
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp</p><br />
</p2><br />
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<p><a name="a1.11" id="a1.11"></a><h4><strong>1.11 Gel Extraction ( Using TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0)</strong></h4><br /><br />
<p2><br />
<p>1.Excise the DNA fragment from the agarose gel with a clean, sharp scalpel. <br /><br />
2. Cut the gel into small pieces by cutting the gel<br /><br />
3. Weigh the gel pieces and calculate the volume of gel. 1 mg of gel is equivalent to a<br /><br />
1 μl volume.<br /><br />
4. Add Buffer GM to gel for melting. The amount of Buffer GM is shown in the table<br /><br />
below.<br /><br />
gel concentration Buffer GM<br /><br />
1.0% 3X sample volume<br /><br />
1.0 - 1.5% 4X sample volume<br /><br />
1.5 - 2.0% 5X sample volume<br /><br />
5. Mix well and melt the gel at room temperature (15 - 25℃). If the concentration of<br /><br />
gel is too high or the gel is hard to melt, warm at 37℃. Intermittent vortexing will<br /><br />
accelerate gel solubilization.<br /><br />
Note: Gel must be completely dissolved, or the DNA fragment recovery will be<br /><br />
reduced. Extend the melting time when the gel concentration is high.<br /><br />
6. Set a Spin Column into Collection Tube.<br /><br />
7. Transfer the solubilized agarose from Step 7 into the column. Centrifuge at 12,000 rpm<br /><br />
for 1 minute. Discard the flow-through.For improvement the recovery rate of DNA, transfer the flow-through to SpinColumn again and centrifuge again.<br /><br />
8. Add 700 μl of Buffer WB into the Spin Column. Centrifuge at 12,000 rpm for 30 seconds.<br /><br />
Discard the flow-through.<br /><br />
Note: Make sure that the amount of 100% ethanol specified on the bottle label has<br /><br />
been added to the Buffer WB.<br /><br />
9. Repeat Step 10.<br /><br />
10. Place the Spin Column back into Collection Tube. Centrifuge at 12,000 rpm for 1 minute.<br /><br />
11. Place the Spin Column into a new 1.5 ml tube. Add 20 μl of 60℃ sterile<br /><br />
distilled water to the center of the membrane. Let it stand for 1 minute at room temperature and then centrifuge.<br /><br />
12.Take back the flow-through to the center of the membrane. Let it stand for 1 minute at room temperature and then centrifuge again. </p><br />
</p2><br />
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</p><br />
<p><a name="a1.12" id="a1.12"></a><h4><strong>1.12 Ligation</strong></h4><br /><br />
<p2><br />
<p>1. Mix the components as follows to prepare a 10 μl reaction mixture:<br /><br />
0.5 μl Water<br /><br />
1 μl 10X ligation buffer<br /><br />
0.5 μl T4 DNA ligase<br /><br />
2 μl Vector<br /><br />
6 μl Insert<br /><br />
2. Incubate the reactions at 16oC overnight, 22oC for 1 h or stand in RT for 10min.</p><br />
</p2><br />
</p><br />
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<p><a name="a2.1" id="a2.1"></a><br />
<h4><strong>2.1 Cell Viability Test with Voltage applied</strong></h4><br />
<p2><br />
<ul><br />
<li>Experimental Groups</li><br />
</ul><br />
<p> Cells used: BL21 transformed with 2µL PSB1C3 + RFP<br />
Spread on Chloramphenicol agar plate and incubate overnight in 37 °C <br /><br />
</p><br />
<ul><br />
<li>Control group</li><br />
</ul><br />
<p> Cells used: BL21 transformed with 2µL water.<br />
Spread on Blank agar plate and incubate overnight in 37 °C </p><br />
<br />
<p> 1)Pick 4 clones from each group into tubes with 10 mL of LB solution. Add 10 µL of Chloramphenicol to experimental group tubes. Do overnight shake in incubator for 12 hours to make the cells saturated.After 12 hours, take out the tubes from the incubator. </p><br />
<p>2)Prepare the following tubes for each control and experimental groups.</p><br />
<table border="1" cellspacing="0" cellpadding="0" width="624"><br />
<tr><br />
<td width="92" valign="top"><p>Test Set</p></td><br />
<td width="70" valign="top"><p>27 V</p></td><br />
<td width="70" valign="top"><p>18 V</p></td><br />
<td width="70" valign="top"><p>9 V</p></td><br />
<td width="70" valign="top"><p>-9V</p></td><br />
<td width="71" valign="top"><p>-18V</p></td><br />
<td width="71" valign="top"><p>-27V</p></td><br />
<td width="109" valign="top"><p>OV( control)</p></td><br />
</tr><br />
<tr><br />
<td width="92" valign="top"><p>1</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="109" valign="top"><p>0.8 mL</p></td><br />
</tr><br />
<tr><br />
<td width="92" valign="top"><p>2</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="109" valign="top"><p>0.8 mL</p></td><br />
</tr><br />
<tr><br />
<td width="92" valign="top"><p>3</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="70" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="71" valign="top"><p>0.8 mL</p></td><br />
<td width="109" valign="top"><p>0.8 mL</p></td><br />
</tr><br />
</table><br />
<p>Then, we add 3.2 mL of LB and 4 µL of Chloramphenicol (35 mg/L) to each of the experimental group tubes. For control group, we only add 3.2 mL of LB. </p><br />
<br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br />
3)Before connecting the voltage to each sample, we take 0.8 mL of each sample to test for initial Optical Density measurement using Spectrophotometry.<br />
<br />
<p>4)Next, we perform the experiment with voltage applied to each tubes. We use one 9V battery for 9V, two 9V batteries in series for 18 V and three 9V batteries in series for 27 V. Tubes will be shaken under 250 rpm and 25°C for 16 hours. </p></p2><br />
</div><br />
<br />
<div id="circuitcontainer"><br />
<div id="Cone" ><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/c/c5/Oneplusnine.jpg" width="361" height="260" /></p><br />
<p><br />
<p2>Fig. 1 +9V circuit</p2><br />
</p><br />
</div><br />
<div id="Ctwo"><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/e/eb/Twominusnine.jpg" width="330" height="242" /></p><br />
<p><br />
<p2> Fig. 2 -9V circuit</p2><br />
</p><br />
</div><br />
<div id="Cfive"><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/3/30/Fiveplustwosev.jpg" width="322" height="424" /></p><br />
<p2>Fig. 5 +27V circuit</p2><br />
</div><br />
<div id="Cfour"><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/6/6c/Fourminuseteen.jpg" width="318" height="347" /></p><br />
<p><br />
<p2>Fig. 4 -18V circuit</p2><br />
</p><br />
</div><br />
<div id="Cthree"><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/5/59/Threepluseteen.jpg" width="322" height="335" /></p><br />
<p><br />
<p2>Fig. 3 +18V circuit</p2><br />
</p><br />
</div><br />
<div id="Csix"><br />
<p><img src="https://static.igem.org/mediawiki/igem.org/e/e2/Sixminustwosev.jpg" width="341" height="439" /></p><br />
<p2>Fig. 6 -27V circuit</p2><br />
</div><br />
</div><br />
<div id="partable2"><br />
<p2><br />
<p><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br><br></br>5)After 16 hours, we take the tubes out. Then we take 0.8 mL of each sample to perform final Optical Density measurement using Spectrophotometry.</p><br />
<p>6)Calculate Changes in Optical Density= Final Optical Density – Initial Optical Density</p><br />
<p>7)Plot the graph for three test sets. </p><br />
<p><a href="#top">Back To Top</a></p><br />
<p><a name="a2.2" id="a2.2"></a><h4><strong>2.2 Cell Viability Test with BaP</strong></h4><br />
<p2><br />
<ol><br />
<li> Transform BL21 cells with pSB1C3-RFP<br /><br />
2. Pick a clone for overnight culture at 37oC<br /><br />
3. Label in triplicate 12 5ml centrifuge tubes, 6 tubes of different concentrations of BaP, the other 6 tubes of different concentrations of quinone.</li><br />
<li> To each of the tubes, add in 750ul of LB solution, 150ul of cells, and 100ul of BaP/Quinone solutions in 10X stock corresponding to the labels<br /><br />
5. Mix well and take an OD600 reading using a microplate reader.</li><br />
<li> Shake the cells in 25oC for 12 hours.</li><br />
<li> Take another reading of OD600.</li><br />
<li> Repeat Step 6-7.</li><br />
<li> Plot a growth curve of cells under different concentrations of BaP/Quinone</li><br />
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2013-10-22T07:19:55Z
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