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From 2013.igem.org

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• 03:25, 28 September 2013 Tkalkus (Talk | contribs) uploaded "File:Trento HisC Growth Curve.pdf" ‎ (We used our knockout cells to take a growth curve of the Trento 2012 CysE gene in a pUC19 vector in knockout cells induced by IPTG and grown in LB for one day.)
• 03:22, 28 September 2013 Tkalkus (Talk | contribs) uploaded "File:CysE Positive Control.jpg" ‎ (CysE knockout strains have been rescued by the Trento 2012 CysE part in a pUC19 expression vector on M9 minimal media plates as shown by this close up picture of the sheet growth.)
• 00:00, 28 September 2013 Tkalkus (Talk | contribs) uploaded "File:HisC CysE Growth Curves.png" ‎ (These graphs represent the growth curves in LB of our rescued HisC positive control cells (top left), rescued CysE positive control cells with the Trento2012 gene (top right), rescued HisC ancestral gene (bottom left), and rescued CysE ancestral gene (bot)
• 23:52, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:DeX process.png" ‎ (The cells on the right all have the gene of interest. DeExtinction is turning back the clock to find the gene that belonged to a common ancestor of these cells.)
• 23:31, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:Negative CysE Control.jpg" ‎ (CysE knockout cells were transformed using an empty pUC19 expression vector and plated on M9 minimal media with IPTG. Nothing grew because the cells could not produce cysteine. This is a negative control for our ancestral CysE gene.)
• 23:19, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:HisC Positive Control.jpg" ‎ (HisC knockout cells were transformed with the pUC19 plasmid with the extant E. coli HisC gene insert and were induced with IPTG. We allowed these cells to grow on M9 minimal media plates, so that only transformed cells would be rescued. This is a positive)
• 22:02, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:CasA Foldit Model.png" ‎ (Using Foldit and the CasA protein from Thermus thermophilus as a baseline framework, we modeled our ancestral CasA protein to get a physical look at the conserved active sites.)
• 09:31, 27 September 2013 Tkalkus (Talk | contribs) uploaded a new version of "File:EvolutionOfLife.png" ‎ (From the formation of organic proteins on a hostile planet to modern man seeking out the stars, this graphic shows the evolution of life as we understand it. )
• 09:29, 27 September 2013 Tkalkus (Talk | contribs) uploaded a new version of "File:EvolutionOfLife.png" ‎ (From the formation of organic proteins on a hostile planet to modern man seeking out the stars, this graphic shows the evolution of life as we understand it.)
• 09:22, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:EvolutionOfLife.png" ‎ (From the formation of organic proteins on a hostile planet to modern man seeking out the stars, this graphic shows the evolution of life as we understand it.)
• 08:10, 27 September 2013 Tkalkus (Talk | contribs) uploaded a new version of "File:Conserved region.png" ‎ (The top large-letter sequence is from the Protein Family Data base with the colored circles showing regions that correspond to CasA protein binding sites. The other three sequences, from bottom to top respectively, are the sequences for our ancestral reco)
• 08:09, 27 September 2013 Tkalkus (Talk | contribs) uploaded "File:Conserved region.png" ‎ (The top large-letter sequence is from the Protein Family Data base with the colored circles showing regions that correspond to CasA protein binding sites. The other three sequences, from bottom to top respectively, are the sequences for our ancestral reco)