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• Jsher
  ... to a gate that had 15 of the baseline (which was 38 or our cells with our plasmid) ...tures of 64, 65, 66 in LB Kan (Kan is 1000x) for MAGE. These all have our plasmid already
  64 KB (10,685 words) - 04:42, 16 January 2014
• J Sher
  ... to a gate that had 15 of the baseline (which was 38 or our cells with our plasmid) ...tures of 64, 65, 66 in LB Kan (Kan is 1000x) for MAGE. These all have our plasmid already
  64 KB (10,685 words) - 04:40, 16 January 2014
• Team:UChicago/Notebook
  :*Plasmid Design *The plasmid design team decided on gibson assembly to produce the kerA biobrick with ge
  68 KB (10,479 words) - 02:05, 28 September 2013
• Team:Waterloo
  ... packaged itself due to a missing packaging sequence is called a “helper plasmid”. </p> ... phagemid” and will be transmitted from sender cells that carry a helper plasmid</p>
  182 KB (26,875 words) - 03:50, 29 October 2013
• Team:BYU Provo/Cholera - Detection
  One possible question is how to get a plasmid to form with medicine or something inside. Today I looked over existing plasmid constructs. Apparently iGEMers from last year did clone many genes into pla
  54 KB (8,926 words) - 20:11, 29 July 2013
• Team:ITB Indonesia/Notebook/WetLab
  Transform the plasmid to DH5α competent cell. We compared number of colony from CaCl2 method an <li>Preparing solutions for plasmid isolation by alkali lysis method</li>
  25 KB (3,936 words) - 03:05, 28 September 2013
• Cholera - Detection
  One possible question is how to get a plasmid to form with medicine or something inside. Today I looked over existing plasmid constructs. Apparently iGEMers from last year did clone many genes into pla
  48 KB (8,065 words) - 22:53, 22 May 2013
• Team:Leicester/Notebook
  <h3 class="nb">Transformation of RFP control plasmid</h3> Negative control (No plasmid)<br />
  117 KB (15,598 words) - 00:32, 5 October 2013
• Team:Heidelberg/Templates/DelH week22
  ===Generation of DelH Plasmid pHM04 18-09=== ===Generation of DelH Plasmid pHM04 20-09===
  21 KB (3,010 words) - 21:36, 25 October 2013
• Team:Frankfurt/Project/Methods
  ... transformation. Also only one restriction enzyme for linearization of the plasmid is needed. <br><br> ...a new way of assembling BioBrick devices in a desired order to a targeting plasmid using homologue recombination system of yeast. It is called ''Yeast BioBric
  23 KB (3,829 words) - 01:55, 5 October 2013
• Team:Freiburg/Notebook/lab hormon
  <li>1 µl of plasmid (~ 20 ng/µl) was added to 25 µl of chemically competent <i>E. coli</i> ce using <i>Roti-Prep Plasmid Mini</i> from Roth
  64 KB (10,342 words) - 05:16, 28 October 2013
• Team:Goettingen/NoteBook w6
  <p class="timeline-title goe-rt">Mini Plasmid Preparation, Test Digest of Clone 12 RBS-GFP-Terminator (see gel 11.07.) wi ...-bidi-font-weight:normal"><span lang="EN-US" style="font-size:11.0pt">Mini Plasmid Preparation<o:p></o:p></span></b></p>
  387 KB (51,437 words) - 21:20, 30 September 2013
• Team:Duke/Notebook/Lab Notebook
  *Matt Farnitano built plasmid iGEM.1 with Golden Gate reaction from backbone, ACT1 promoter, YEVenus repo *CC built plasmid iGEM.2 with Golden Gate reaction using truncated ACT1 promoter
  66 KB (11,234 words) - 01:40, 28 September 2013
• Team:Heidelberg/Project/Delftibactin
  ...thereby avoid the intricacies expected for cloning of a single 59&nbsp;kbp plasmid as well as to allow for faster trouble shooting in case issues with the clo <li>Plasmid: methylmalonyl-CoA pathway, PPTase sfp & permeability device <a href='http:
  66 KB (9,751 words) - 03:53, 29 October 2013
• Team:UGent/Labjournal
  <li> General techniques: plasmid/PCR purification, inoculation, gel extraction, restriction & ligation. </li <li> <b>Purify plasmid pTGD-<i>ccdA</i>-Pmb1GFP-CmFRT</b> using Qiagen spin mini kit: Nanodrop --
  45 KB (7,829 words) - 01:35, 5 October 2013
• Team:KU Leuven/Journal/EBF/wetlab
  ... for the unsuccessfully transformed biobricks. Later we also performed the plasmid extraction on all the successfully transformed biobricks.<br/> ... 3 ml LB medium, then isolated the plasmid and cut the backbone out of the plasmid. To make sure that we have enough backbone we did this in quadruple. Before
  64 KB (10,721 words) - 03:34, 29 October 2013
• Team:Freiburg/Project/induction
  ...//2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a></b>. This plasmid codes for the RNAs that are necessary for the binding ability of dCas9. <td> <b>Figure 1: Plasmid-cards of red-light effector-control plasmids </b><br>
  46 KB (7,044 words) - 03:50, 29 October 2013
• Team:Cornell/notebook
  |tech=From the plasmid pUCATPH (pAh13G), we amplified the ''trpC'' promoter (P''trpC'', a constitu ... author flyouts I implemented yesterday, as well as other keywords such as plasmid names and protocols.
  179 KB (31,118 words) - 03:34, 29 October 2013
• Team:Freiburg/Project/crrna
  ... to be cotransfected with the dCas9-plasmids. Therefore we designed an RNA plasmid, termed <b><a id="link" href="https://2013.igem.org/Team:Freiburg/Project/c ...ed using the iGEM BioBrick cloning strategy thereby allowing for a RNAimer plasmid carrying multiple crRNAs.<br>
  35 KB (5,041 words) - 03:59, 29 October 2013
• Team:Macquarie Australia/Notebook
  ...esults indicated that all the genes had similar intensities to the control plasmid and vectors, except for ChlM, which was slightly lower. ...ed. The results indicated that the gene was successfully inserted into the plasmid vector.
  46 KB (7,422 words) - 03:48, 28 September 2013