Team:BGU Israel/Achievements

Achievements

Achievements

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Biobricks

The following biobricks were designed, sent to the registry and characterized:

1. Bba_K1223002 – P.A.S.E 2 cassette
2. Bba_K1223003 - KanR (promoter+CDS)
3. Bba_K1223005 - cI translational unit
4. Bba_K1223006 - HisTag + stop codon
5. Bba_K1223007 - cI translational unit with His-tag
6. Bba_K1223011 - ampR translational unit (ampicilin resistance CDS+promoter)

P.A.S.E 1

1. The system’s parts have been designed and synthesized.
2. 2 out of 3 of the system’s parts have been assembled in e. coli – pkd78 (recombination system) and pUC57 cI (protective element)
3. Since we haven’t succeeded yet in proving the recombination system works, an alternative design was conceived for purposes of proof of concept, relying on the same principles as the original design. Instead of using a linear DNA with recombination sites for the toxin cassette, we used a plasmid as a cloning vector. The completed system was assembled and it is comprised of an e. coli transformed with pUC57 cI and pUC57 Toxin (toxin regulated by cI binding promoter). Characterizing the system is undergoing.

P.A.S.E 2

1. The system’s parts have been designed and synthesized.
2. A recombination system (pkd78) with different antibiotic resistance have been constructed (we replaced the chloramphenicol resistance of pkd78 with carbenicillin resistance).