http://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&feed=atom&action=historyTeam:BGU Israel/Solution - Revision history2024-03-29T00:45:41ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=357386&oldid=prevNeta.weiss at 01:36, 29 October 20132013-10-29T01:36:52Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h6>Continue the journey: read about <a href="/Team:BGU_Israel/Bricks"><del class="diffchange diffchange-inline">Our </del>Bio Bricks</a>.</h6></br></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h6>Continue the journey: read about <ins class="diffchange diffchange-inline">Our </ins><a href="/Team:BGU_Israel/Bricks"> Bio Bricks</a>.</h6></br></br></div></td></tr>
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</table>Neta.weisshttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=355361&oldid=prevNeta.weiss at 00:25, 29 October 20132013-10-29T00:25:39Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h6>Continue the journey: read about <del class="diffchange diffchange-inline"><li></del><a href="/Team:BGU_Israel/Bricks">Our Bio Bricks</a></<del class="diffchange diffchange-inline">li</del>><del class="diffchange diffchange-inline">.</del></<del class="diffchange diffchange-inline">h6</del>></br></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h6>Continue the journey: read about <a href="/Team:BGU_Israel/Bricks">Our Bio Bricks</a><ins class="diffchange diffchange-inline">.</ins></<ins class="diffchange diffchange-inline">h6</ins>></<ins class="diffchange diffchange-inline">br</ins>></br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>References:</b></br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>References:</b></br></br></div></td></tr>
</table>Neta.weisshttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=355327&oldid=prevNeta.weiss at 00:24, 29 October 20132013-10-29T00:24:09Z<p></p>
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</table>Neta.weisshttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331786&oldid=prevStav shamir at 14:31, 26 October 20132013-10-26T14:31:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>[2]</b> McLoughlin, A. J. (1994). “Plasmid stability and ecological competence in recombinant cultures”. Biotechnology Advances (12), 279–324. </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>[2]</b> McLoughlin, A. J. (1994). “Plasmid stability and ecological competence in recombinant cultures”. Biotechnology Advances (12), 279–324. </br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>[3] </b>Datsenko K. A., Wanner, B.L. (2000). “One-step inactivation of chromosomal genes in Escherichia coliK-12 using PCR products”. PNAS (97), 6640-6645.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <b>[3] </b>Datsenko K. A., Wanner, B.L. (2000). “One-step inactivation of chromosomal genes in Escherichia coliK-12 using PCR products”. PNAS (97), 6640-6645.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </br></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b>[4]</b> J Biotechnol. 2007 Feb 1;128(2):362-75. Epub 2006 Oct 17. Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives. Portle S, Causey TB, Wolf K, Bennett GN, San KY, Mantzaris N.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2013/e/ef/BGU_1-s2.0-S0168165606008613-main_-1-.pdf" target="_blank">View Source</a></br></ins></div></td></tr>
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</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331642&oldid=prevStav shamir at 14:06, 26 October 20132013-10-26T14:06:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a lot of experiments (detailed in the July section of our lab notebook) and further theoretical research we came to two conclusions – the lacI/lacO is very “leaky” and the inducer concentration has no effect on the levels of expression [4], despite what our results have shown. We learned that different levels of fluorescence achieved by different inducer concentration, are the not the result of different expression levels on the single cell basis – they are the outcome of a bimodal distribution. All cells are either “on” (induced) or “off”. The ratio between the cells which are on and the cells which are of is the reason for different fluorescence measured.</br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a lot of experiments (detailed in the July section of our lab notebook) and further theoretical research we came to two conclusions – the lacI/lacO is very “leaky” and the inducer concentration has no effect on the levels of expression [4], despite what our results have shown. We learned that different levels of fluorescence achieved by different inducer concentration, are the not the result of different expression levels on the single cell basis – they are the outcome of a bimodal distribution. All cells are either “on” (induced) or “off”. The ratio between the cells which are on and the cells which are of is the reason for different fluorescence measured.</br></br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We wanted to reproduce the experiments that lead to this conclusion, so we used FACS analysis on our e. coli with pGFPuv. The FACS was done on 3 different cultures: IPTG induced BL21 pGFPuv, uninduced BL21 pGFPuv, and uninduced BL21. </br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We wanted to reproduce the experiments that lead to this conclusion, so we used FACS analysis on our e. coli with pGFPuv. The FACS was done on 3 different cultures: IPTG induced BL21 pGFPuv, uninduced BL21 pGFPuv, and uninduced BL21. </br><<ins class="diffchange diffchange-inline">/br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">center</del>><img src="https://static.igem.org/mediawiki/2013/f/fd/BGU_FACS_RESULTS.JPG" /></<del class="diffchange diffchange-inline">center</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/f/fd/BGU_FACS_RESULTS.JPG" /></<ins class="diffchange diffchange-inline">br></br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">These results we produced show that the conclusion mentioned above is correct (x axis is fluorescence, y axis is cell count). The left graph shows that the induced culture could be divided to two sub populations – one which expressed GFP, and one that didn’t. The middle graph shows that even when uninduced, most of the cells do produce GFP, which supports the fact this regulation system is leaky.</br></br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We had to return to the drawing table. After a thorough searching session we found an interesting promoter that might suit our 2 goals (tight & programmable) – lac/ara-1 promoter – part BBa_K354000. This promoter is activated by induction with IPTG and/or L - arabinose. [1] This promoter functions as a fourway switch - using different variation of inducers (IPTG/Arabinsoe/IPTG+Arabinose/no inducer) allows the user to adjust the induction strength and levels of expression. We also thought since this promoter has binding sites for two repressors instead of just one, it would be tightly regulated.</br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We also found a toxin and the suitable repressor/promoter, as detailed below.</br</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td></tr>
</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331595&oldid=prevStav shamir at 14:00, 26 October 20132013-10-26T14:00:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a lot of experiments (detailed in the July section of our lab notebook) and further theoretical research we came to two conclusions – the lacI/lacO is very “leaky” and the inducer concentration has no effect on the levels of expression [4], despite what our results have shown. We learned that different levels of fluorescence achieved by different inducer concentration, are the not the result of different expression levels on the single cell basis – they are the outcome of a bimodal distribution. All cells are either “on” (induced) or “off”. The ratio between the cells which are on and the cells which are of is the reason for different fluorescence measured.</br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a lot of experiments (detailed in the July section of our lab notebook) and further theoretical research we came to two conclusions – the lacI/lacO is very “leaky” and the inducer concentration has no effect on the levels of expression [4], despite what our results have shown. We learned that different levels of fluorescence achieved by different inducer concentration, are the not the result of different expression levels on the single cell basis – they are the outcome of a bimodal distribution. All cells are either “on” (induced) or “off”. The ratio between the cells which are on and the cells which are of is the reason for different fluorescence measured.</br></br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We wanted to reproduce the experiments that lead to this conclusion, so we used FACS analysis on our e. coli with pGFPuv. The FACS was done on 3 different cultures: IPTG induced BL21 pGFPuv, uninduced BL21 pGFPuv, and uninduced BL21. </br></<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We wanted to reproduce the experiments that lead to this conclusion, so we used FACS analysis on our e. coli with pGFPuv. The FACS was done on 3 different cultures: IPTG induced BL21 pGFPuv, uninduced BL21 pGFPuv, and uninduced BL21. </br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">center><img src="https://static.igem.org/mediawiki/2013/f/fd/BGU_FACS_RESULTS.JPG" </ins>/><ins class="diffchange diffchange-inline"></center></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td></tr>
</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331517&oldid=prevStav shamir at 13:43, 26 October 20132013-10-26T13:43:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>While we had to look for the first two parts, it was rather obvious for us what we should use for the third – the famous lacI/lacO from the lac operon we all learned about in Microbiology 101. We were eager to work, and it happened so that the lab we worked in had a stock of pGFPuv – a plasmid containing GFP gene under the regulation of lacO. So, we started conducting a long series of experiments with two goals:</br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>While we had to look for the first two parts, it was rather obvious for us what we should use for the third – the famous lacI/lacO from the lac operon we all learned about in Microbiology 101. We were eager to work, and it happened so that the lab we worked in had a stock of pGFPuv – a plasmid containing GFP gene under the regulation of lacO. So, we started conducting a long series of experiments with two goals:</br></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Verifying that lacI/lacO is tightly regulating the gene under its control. </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Verifying that lacI/lacO is tightly regulating the gene under its control. </br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. <ins class="diffchange diffchange-inline"></br></br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">After a lot of experiments (detailed in the July section of our lab notebook) and further theoretical research we came to two conclusions – the lacI/lacO is very “leaky” and the inducer concentration has no effect on the levels of expression [4], despite what our results have shown. We learned that different levels of fluorescence achieved by different inducer concentration, are the not the result of different expression levels on the single cell basis – they are the outcome of a bimodal distribution. All cells are either “on” (induced) or “off”. The ratio between the cells which are on and the cells which are of is the reason for different fluorescence measured.</br></br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We wanted to reproduce the experiments that lead to this conclusion, so we used FACS analysis on our e. coli with pGFPuv. The FACS was done on 3 different cultures: IPTG induced BL21 pGFPuv, uninduced BL21 pGFPuv, and uninduced BL21. </br></ins></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331504&oldid=prevStav shamir at 13:40, 26 October 20132013-10-26T13:40:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br></br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We knew what kind of parts we need:</br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We knew what kind of parts we need:<ins class="diffchange diffchange-inline"></br></ins></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. A toxin </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. A toxin </br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. A repressor/promoter couple to regulate the toxin expression </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. A repressor/promoter couple to regulate the toxin expression </br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. A regulation system to control the expression of the toxin regulating repressor </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. A regulation system to control the expression of the toxin regulating repressor </br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">While we had to look for the first two parts, it was rather obvious for us what we should use for the third – the famous lacI/lacO from the lac operon we all learned about in Microbiology 101. We were eager to work, and it happened so that the lab we worked in had a stock of pGFPuv – a plasmid containing GFP gene under the regulation of lacO. So, we started conducting a long series of experiments with two goals:</br></br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Verifying that lacI/lacO is tightly regulating the gene under its control. </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Quantifying the different level of expression under different inducer (IPTG in this case) concentrations – in order to show our system could be potentially “programmable”. </br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td></tr>
</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331494&oldid=prevStav shamir at 13:38, 26 October 20132013-10-26T13:38:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br></br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We knew what kind of parts we need:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We knew what kind of parts we need:<<ins class="diffchange diffchange-inline">/br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><<del class="diffchange diffchange-inline">ol class="ablist"</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. </ins>A toxin </<ins class="diffchange diffchange-inline">br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><li class="ablist"></del>A toxin</<del class="diffchange diffchange-inline">li</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. </ins>A repressor/promoter couple to regulate the toxin expression </<ins class="diffchange diffchange-inline">br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><li class="ablist"></del>A repressor/promoter couple to regulate the toxin expression</<del class="diffchange diffchange-inline">li</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. </ins>A regulation system to control the expression of the toxin regulating repressor </br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><li class="ablist"></del>A regulation system to control the expression of the toxin regulating <del class="diffchange diffchange-inline"> </del>repressor<del class="diffchange diffchange-inline"></li></del></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"></ol></del></br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Detailed design </h4><hr/></br></div></td></tr>
</table>Stav shamirhttp://2013.igem.org/wiki/index.php?title=Team:BGU_Israel/Solution&diff=331426&oldid=prevStav shamir at 13:22, 26 October 20132013-10-26T13:22:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Behind the Design</h4><hr/></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h4>Behind the Design</h4><hr/></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br> <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> After having decided what our project will be, we sat down and devised a general mechanism to make it work, as described in the overview above. It seemed to us, at that point, that we have done a great deal of the planning. But as we started looking for actual parts and biobricks that we could use to make our plan a reality, we realized the real challenge was still ahead of us – the registry is so big, that there is simply too much to choose from!</br><ins class="diffchange diffchange-inline"></br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We knew what kind of parts we need: <del class="diffchange diffchange-inline"></br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We knew what kind of parts we need:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ol class="ablist"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ol class="ablist"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class="ablist">A toxin</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class="ablist">A toxin</li></div></td></tr>
</table>Stav shamir